Resveratrol Increases Osteoblast Differentiation In Vitro Independently of Inflammation.

Abstract:

:Low-grade inflammation negatively affects bone. Resveratrol is a natural compound proven to possess both anti-inflammatory and bone protective properties. However, it is uncertain if the bone effects are mediated though anti-inflammatory effects. Firstly, we investigated if resveratrol affects proliferation and differentiation of human bone marrow-derived mesenchymal stem cells. Secondly, we investigated if inflammation negatively affects proliferation and differentiation, and if resveratrol counteracts this through anti-inflammatory effects. Mesenchymal stem cells were obtained from bone marrow aspiration in 13 healthy individuals and cultured towards the osteoblast cell lineage. The cells were stimulated with resveratrol, lipopolysaccharide (LPS), LPS + resveratrol, or vehicle (control) for 21 days. Compared to control, resveratrol decreased cell number by 35 % (p < 0.05) and induced differentiation (a 3-fold increase in alkaline phosphatase (p < 0.002), while P1NP and OPG showed similar trends). LPS induced inflammation with a 44-fold increase in interleukin-6 (p < 0.05) and an extremely prominent increase in interleukin-8 production (p < 0.05) relative to control. In addition, LPS increased cell count (p < 0.05) and decreased differentiation (a reduction in P1NP production (p < 0.02)). Co-stimulation with LPS + resveratrol did not reduce interleukin-6 or interleukin-8, but nonetheless, cell count was reduced (p < 0.05) and alkaline phosphatase, P1NP, and OPG increased (p < 0.05 for all). Thus, resveratrol stimulates osteoblast differentiation independently of inflammation.

journal_name

Calcif Tissue Int

authors

Ornstrup MJ,Harsløf T,Sørensen L,Stenkjær L,Langdahl BL,Pedersen SB

doi

10.1007/s00223-016-0130-x

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

155-63

issue

2

eissn

0171-967X

issn

1432-0827

pii

10.1007/s00223-016-0130-x

journal_volume

99

pub_type

杂志文章
  • Absence of linkage for bone mineral density to chromosome 12q12-14 in the region of the vitamin D receptor gene.

    abstract::Polymorphisms in the region of the gene for the vitamin D receptor (VDR) (chromosome 12q12-14) have been associated with differences in bone mineral density (BMD) in some studies but not in others. Because linkage analysis assesses allele sharing identical-by-descent among relatives instead of the association of a par...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002230001175

    authors: Zee RY,Myers RH,Hannan MT,Wilson PW,Ordovas JM,Schaefer EJ,Lindpaintner K,Kiel DP

    更新日期:2000-12-01 00:00:00

  • Mitogenic action of hydrochlorothiazide on human osteoblasts in vitro: requirement for platelet-derived growth factor.

    abstract::Long-term use of hydrochlorothiazide (HCTZ), a common diuretic agent for hypertension, has been associated with increased bone density and reduced hip fracture rates in patients. In this study, we sought to examine whether HCTZ has an anabolic effect on the proliferation of human osteoblasts (derived from either verte...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00369219

    authors: Lau KH,Song XD,Ochi M,Wergedal JE

    更新日期:1996-12-01 00:00:00

  • Prospects for Therapies in Osteoarthritis.

    abstract::Osteoarthritis (OA) is a chronic, debilitating disease affecting millions of people worldwide. Management of OA involves pharmacological and non-pharmacological approaches. Conventional pharmacological treatments have limited efficacy and are associated with a number of side-effects, restricting the number of patients...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审

    doi:10.1007/s00223-020-00672-9

    authors: Ghouri A,Conaghan PG

    更新日期:2020-02-13 00:00:00

  • Fracture risk associated with different types of oral corticosteroids and effect of termination of corticosteroids on the risk of fractures.

    abstract::We conducted a case-control study on fracture risk associated with the use of orally administered prednisolone/prednisone, budesonide, methylprednisolone, and hydrocortisone to assess if the various preparations were associated with different fracture patterns. Cases were all subjects with any fracture sustained durin...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-008-9124-7

    authors: Vestergaard P,Rejnmark L,Mosekilde L

    更新日期:2008-04-01 00:00:00

  • TGF-beta/extracellular matrix interactions in dentin matrix: a role in regulating sequestration and protection of bioactivity.

    abstract::TGF-beta isoforms sequestrated in dentin matrix potentially provide a reservoir of bioactive molecules that may influence cell behavior in the dentin-pulp complex following tissue injury. The association of these growth factors with dentin matrix and the influence of such associations on the bioactivity of growth fact...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-009-9248-4

    authors: Baker SM,Sugars RV,Wendel M,Smith AJ,Waddington RJ,Cooper PR,Sloan AJ

    更新日期:2009-07-01 00:00:00

  • Incident Fracture Risk in Type 2 Diabetic Postmenopausal Women in Mainland China: Peking Vertebral Fracture Study.

    abstract::Using data from the Peking Vertebral Fracture Study, we conducted a longitudinal cohort study to investigate the association between type 2 diabetes mellitus (T2DM) and the risk of incident fractures, especially of vertebral fractures (VFs), and we also examined the modifying effect of body mass index (BMI) on this as...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-019-00598-x

    authors: Jiajue R,Qi X,Jiang Y,Wang Q,Wang W,Pei Y,Wang X,Huang W,Zheng X,Ning Z,Wang O,Li M,Xing X,Yu W,Xu L,Xia W

    更新日期:2019-11-01 00:00:00

  • Ultrasound and densitometry of the calcaneus correlate with the failure loads of cadaveric femurs.

    abstract::We assessed the bone mineral density (BMD) of 16 matched sets of cadaveric proximal femurs and feet using dual-energy x-ray absorptiometry (DXA). We also estimated the femoral neck length from the DXA scans. Quantitative ultrasound densitometry was used to measure the velocity of sound and broadband ultrasound attenua...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00296338

    authors: Bouxsein ML,Courtney AC,Hayes WC

    更新日期:1995-02-01 00:00:00

  • Effects of aluminum(III), chromium(III), and iron(III) on the rate of dissolution of calcium hydroxyapatite crystals in the absence and presence of the chelating agent desferrioxamine.

    abstract::Aluminum ions (Al) and chromium (III) ions (Cr), as they exist in aqueous solution at neutral pH, adsorb onto calcium hydroxyapatite crystals (HAP) and severely inhibit their dissolution process, when present in concentrations less than 1 microM. Iron (III) ions (Fe), at concentrations up to 10 microM, have no effect ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555127

    authors: Christoffersen MR,Thyregod HC,Christoffersen J

    更新日期:1987-07-01 00:00:00

  • Effects of parathyroid hormone on odontogenesis of the mouse embryonic molar tooth in vitro.

    abstract::Mandibular first molars of 17-day-old mouse embryos were cultured in vitro to examine the histological effects of various concentrations of parathyroid hormone (PTH) on odontogenesis of the molars. PTH did not affect the cytodifferentiation of mesenchymal cells into preodontoblasts but inhibited that of preodontoblast...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555728

    authors: Sakakura Y

    更新日期:1987-01-01 00:00:00

  • Densitometric study of developing femur.

    abstract::The purpose of this work was to extend to long bones the study on the ossification of human fetal skeleton in relation to conceptual age by a quantitative methodological approach. Postero-anterior scans were performed on 29 dried fetal femora (from 11.5 weeks of conceptual age to term) by a Hologic QDR 1000 X-ray dens...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002239900591

    authors: Panattoni GL,D'Amelio P,Di Stefano M,Sciolla A,Isaia GC

    更新日期:1999-02-01 00:00:00

  • Material damping for monitoring of density and strength of bones.

    abstract::The aim of this work was a preliminary assessment of the feasibility of using in vivo measurements of mechanical properties of bones to detect mineral loss and further to relate them to the tendency of the bone to fracture in the case of loss of minerals, such as in osteoporosis. Previous studies of bone strength in v...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00298727

    authors: Dimarogonas AD,Abbasi-Jahromi SH,Avioli LV

    更新日期:1993-03-01 00:00:00

  • Effect of fluoride on parathyroid activity of normal and calcium-deficient rats.

    abstract::Forty 5-week-old rats were randomly divided into 1 control group given a normal diet and water low in fluoride (0.026 mM/1) and experimental group receiving the same basic diet and a fluoride supplement of 2.6 mMol/l in the drinking water. After 30 weeks, half of the fluoride-supplemented and half of the nonsupplement...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02405089

    authors: Rosenquist JB,Lorentzon PR,Boquist LL

    更新日期:1983-07-01 00:00:00

  • Skeletal demineralization in Turner's syndrome.

    abstract::The bone mineral status of 17 girls with Turner's syndrome was evaluated by single photon absorptiometry. Bone mineral content (BMC) was 25.4% below that predicted by normalization for age, sex, height, weight, and bone width. Only 25% of this demineralization could be attributed to delayed skeletal maturation. Bones ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02411296

    authors: Shore RM,Chesney RW,Mazess RB,Rose PG,Bargman GJ

    更新日期:1982-01-01 00:00:00

  • SULT1E1 genetic polymorphisms modified the association between phytoestrogen consumption and bone mineral density in healthy Korean women.

    abstract::Sulfotransferase 1E1 (SULT1E1) catalyze estrogen into sulfate conjugation and is involved in the metabolism of phytoestrogen. A community-based cross-sectional study was conducted on 397 Korean women, to evaluate the association between genetic polymorphisms of SULT1E1 and bone mineral density (BMD) and the combined e...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-006-0008-4

    authors: Lee SA,Choi JY,Shin CS,Hong YC,Chung H,Kang D

    更新日期:2006-09-01 00:00:00

  • Resorption of the mouse incisor after the application of cold to the periodontal attachment apparatus.

    abstract::In order to study in detail the processes leading to the resorption and ankylosis of teeth after trauma, the effects of cold application on the periodontal tissues were studied in the mouse. Liquid nitrogen was applied locally to the outer surface of the lower jaw which resulted in a freezing of the incisor and its su...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02555735

    authors: Wesselink PR,Beertsen W,Everts V

    更新日期:1986-07-01 00:00:00

  • Biochemical differences between dystrophic calcification of cross-linked collagen implants and mineralization during bone induction.

    abstract::Ectopic calcification of diseased tissues or around prosthetic implants can lead to serious disability. Therefore, calcification of implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen was compared with mineralization induced by demineralized bone matrix (DBM). Whereas implants of DBM...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02556366

    authors: Nimni ME,Bernick S,Cheung DT,Ertl DC,Nishimoto SK,Paule WJ,Salka C,Strates BS

    更新日期:1988-05-01 00:00:00

  • Taxol inhibits osteoclastic bone resorption.

    abstract::We have examined the effect of the anti-tumor compound taxol, on osteoclastic bone resorption. In the bone slice assay, taxol (0.1-0.001 microM) dose-dependently inhibited bone resorption with an IC50 of 0.08 microM. Osteoclast survival on bone slices was unaffected by 0.01-1 microM taxol, but 10 microM was cytotoxic....

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00301951

    authors: Hall TJ,Jeker H,Schaueblin M

    更新日期:1995-12-01 00:00:00

  • Reduction in fracture rate and back pain and increased quality of life in postmenopausal women treated with teriparatide: 18-month data from the European Forsteo Observational Study (EFOS).

    abstract::The European Forsteo Observational Study was designed to examine the effectiveness of teriparatide in postmenopausal women with osteoporosis treated for up to 18 months in normal clinical practice in eight European countries. The incidence of clinical vertebral and nonvertebral fragility fractures, back pain, and heal...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-009-9299-6

    authors: Langdahl BL,Rajzbaum G,Jakob F,Karras D,Ljunggren O,Lems WF,Fahrleitner-Pammer A,Walsh JB,Barker C,Kutahov A,Marin F

    更新日期:2009-12-01 00:00:00

  • Opposing changes in osteocalcin levels in bone vs serum during the acquisition of peak bone density in C3H/HeJ and C57BL/6J mice.

    abstract::Our knowledge of the developmental changes in the concentration of serum and bone osteocalcin (OC) is limited. To investigate the interrelationship between skeletal and circulatory OC during acquisition of peak bone density in mice, we examined the temporal changes in the concentration of serum and bone OC from 3 to 1...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-001-1106-y

    authors: Li X,Srivastava AK,Gu W,Masinde G,Mohan S,Baylink DJ

    更新日期:2002-11-01 00:00:00

  • An in vitro and in vivo study of cytokines in the acute-phase response associated with bisphosphonates.

    abstract::We studied the acute phase response, including specific cytokine production, [interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor alpha(TNF alpha)] following a single dose of Aredia (disodium pamidronate) in patients with increased bone turnover and, in vitro, the role played by specific cytokines in the...

    journal_title:Calcified tissue international

    pub_type: 临床试验,杂志文章

    doi:10.1007/s002239900353

    authors: Thiébaud D,Sauty A,Burckhardt P,Leuenberger P,Sitzler L,Green JR,Kandra A,Zieschang J,Ibarra de Palacios P

    更新日期:1997-11-01 00:00:00

  • Restrictive Eating Disorders and Skeletal Health in Adolescent Girls and Young Women.

    abstract::This article reviews the effects of restrictive eating disorders on bone health. The relationship between eating disorders and amenorrhea is discussed in detail. The pathologic impact of malnutrition on bone is explored by examining the results of studies using various available imaging techniques. The multiple hormon...

    journal_title:Calcified tissue international

    pub_type: 杂志文章,评审

    doi:10.1007/s00223-016-0164-0

    authors: Thornton D,Gordon CM

    更新日期:2017-05-01 00:00:00

  • Effect of diphosphonates on glycogen content of rabbit ear cartilage cells in culture.

    abstract::The diphosphonates dichloromethanediphosphonate (Cl2MDP) and to a minor degree 1-hydroxyethane-1,1-diphosphonate (EDHP) increased the glycogen content in cultured cartilage cells of rabbit ear. This effect was not due to different concentrations of glucose in the medium. ...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02408621

    authors: Felix R,Fast DK,Sallis JD,Fleisch H

    更新日期:1980-01-01 00:00:00

  • Hydrogen peroxide induces apoptosis of osteocytes: involvement of calcium ion and caspase activity.

    abstract::We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activatio...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-001-1110-2

    authors: Kikuyama A,Fukuda K,Mori S,Okada M,Yamaguchi H,Hamanishi C

    更新日期:2002-09-01 00:00:00

  • Increase in tartrate-resistant acid phosphatase of bone at the early stage of ascorbic acid deficiency in the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat.

    abstract::The effect of ascorbic acid deficiency on bone metabolism was evaluated using the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat model. Ascorbic acid (Asc)-deficient rats gained body weight in a manner similar to Asc-supplemented rats (control) during 3 weeks, but began to lose weight during the 4th week o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-002-2040-3

    authors: Goto A,Tsukamoto I

    更新日期:2003-08-01 00:00:00

  • Detection of mature collagen in human dental enamel.

    abstract::Mature dental enamel is the most mineralized of all mammalian tissues and considered to be free of collagen. Hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) are two nonreducible cross-links of mature collagen. Hydroxyproline (Hyp) is an amino acid that is believed to be indicative of the presence of collagen....

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-004-0122-0

    authors: Açil Y,Mobasseri AE,Warnke PH,Terheyden H,Wiltfang J,Springer I

    更新日期:2005-02-01 00:00:00

  • A pediatric bone mass scan has poor ability to predict adult bone mass: a 28-year prospective study in 214 children.

    abstract::As the correlation of bone mass from childhood to adulthood is unclear, we conducted a long-term prospective observational study to determine if a pediatric bone mass scan could predict adult bone mass. We measured cortical bone mineral content (BMC [g]), bone mineral density (BMD [g/cm(2)]), and bone width (cm) in th...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-013-9802-y

    authors: Buttazzoni C,Rosengren BE,Tveit M,Landin L,Nilsson JÅ,Karlsson MK

    更新日期:2014-02-01 00:00:00

  • Fourier-transform infrared spectroscopic study of Ca2+-binding to osteocalcin.

    abstract::Osteocalcin, the gamma-carboxyglutamic acid-containing protein, which is the most abundant noncollagenous protein of bone and dentin, is considered to play roles in bone formation and remodeling. It is unclear how the gamma-carboxyglutamic acid side-chains in osteocalcin coordinate to Ca2+, since the X-ray structure o...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s002230010042

    authors: Mizuguchi M,Fujisawa R,Nara M,Nitta K,Kawano K

    更新日期:2001-12-01 00:00:00

  • 1,25-dihydroxyvitamin D3 and the regulation of macrophage function.

    abstract::Vitamin D3 deficient (D-) mice show a depressed inflammatory response and both inflammatory peritoneal macrophages and bone marrow polymorphonuclear leukocytes of D- mice exhibit a decreased spontaneous migration under agarose. The impaired phagocytic response of peritoneal macrophages from D- mice can be corrected by...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF02409507

    authors: Bar-Shavit Z,Noff D,Edelstein S,Meyer M,Shibolet S,Goldman R

    更新日期:1981-01-01 00:00:00

  • The effects of bisphosphonates on the resorption cycle of isolated osteoclasts.

    abstract::Binding sites for wheat germ agglutinin (WGA)-lectin have been shown to become revealed in the demineralized resorption lacunae that osteoclasts excavate on bone substrate. Peroxidase-conjugated WGA-lectin, which binds to bone matrix glycoconjugates and proteoglycans, was used in pit formation assays to assess the act...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/BF00299317

    authors: Selander K,Lehenkari P,Väänänen HK

    更新日期:1994-11-01 00:00:00

  • Anatomical Distribution of Ochronotic Pigment in Alkaptonuric Mice is Associated with Calcified Cartilage Chondrocytes at Osteochondral Interfaces.

    abstract::Alkaptonuria (AKU) is characterised by increased circulating homogentisic acid and deposition of ochronotic pigment in collagen-rich connective tissues (ochronosis), stiffening the tissue. This process over many years leads to a painful and severe osteoarthropathy, particularly affecting the cartilage of the spine and...

    journal_title:Calcified tissue international

    pub_type: 杂志文章

    doi:10.1007/s00223-020-00764-6

    authors: Hughes JH,Keenan CM,Sutherland H,Edwards HR,Wilson PJM,Ranganath LR,Jarvis JC,Bou-Gharios G,Gallagher JA

    更新日期:2021-02-01 00:00:00