Identification of genes for sulfolipid synthesis in primitive red alga Cyanidioschyzon merolae.

abstract：:Using antibodies to the procollagen C-proteinase enhancer of mouse fibroblast culture medium, we have screened by immunoblotting extracts of several post natal mouse and rat tissues for the presence of the enhancer antigen. All rodent connective tissues were relatively rich in enhancer; lower amounts were found in ske...

journal_title：Biochemical and biophysical research communications

authors： Kessler E,Mould AP,Hulmes DJ

更新日期：1990-11-30 00:00:00

abstract：:The arylamide 2-acetylaminofluorene (AAF) is a powerful carcinogen displaying a marked promoting activity, also known to regulate expression of liver detoxifying proteins. In this study we identified CYP3A23, a major inducible cytochrome P-450 (CYP) isoform, as an AAF target in hepatocytes. Indeed, exposure to AAF of ...

journal_title：Biochemical and biophysical research communications

authors： Sparfel L,Payen L,Gilot D,Sidaway J,Morel F,Guillouzo A,Fardel O

更新日期：2003-01-10 00:00:00

abstract：:The production of hydroxyl radicals by tetrachlorohydroquinone (TCHQ, a major metabolite of the widely used biocide pentachlorophenol) in the presence of H(2)O(2) was studied by salicylate hydroxylation method. HPLC with electrochemical detection was used to measure the levels of 2,3- and 2,5-dihydroxybenzoic acid (DH...

journal_title：Biochemical and biophysical research communications

authors： Zhu BZ,Kitrossky N,Chevion M

更新日期：2000-04-21 00:00:00

abstract：:In order to identify the mechanism by which cyclic AMP stimulates expression of the human renin gene (REN), the effect of forskolin was tested in transient expression analyses of REN 5'-flanking DNA-chloramphenicol acetyltransferase (CAT) reporter gene constructs in secondary cultures of human chorio-decidual cells, a...

journal_title：Biochemical and biophysical research communications

authors： Smith DL,Morris BJ,Do YS,Law RE,Shaw KJ,Hseuh WA

更新日期：1994-04-15 00:00:00

abstract：:To delineate the functional importance of the highly conserved triplet amino acid sequence, Asp-Arg-Tyr (DRY) among G protein-coupled receptors in the second intracellular loop, these residues of rat angiotensin II (Ang II) receptor type 1A (AT(1A)) were changed by alanine or glycine by site-directed mutagenesis. Thes...

journal_title：Biochemical and biophysical research communications

authors： Ohyama K,Yamano Y,Sano T,Nakagomi Y,Wada M,Inagami T

更新日期：2002-03-29 00:00:00

abstract：:An ubiquitous biochemical pathway known to synthesize nitric oxide (NO) from L-arginine has been identified in many cell types. Recent studies indicate that besides activating soluble guanylate cyclase NO is likely to have effects unrelated to the known signal transduction pathway. Activation of the soluble NO synthas...

journal_title：Biochemical and biophysical research communications

authors： Dimmeler S,Brüne B

更新日期：1991-08-15 00:00:00

abstract：:We report the usefulness of an impedance-based label-free whole cell assay to identify new ligands for G protein-coupled receptors (GPCRs) involved in microglial cell migration. Authentic GPCR ligands were subjected to the impedance-based cell assay in order to examine the responses of ligands for MG5 mouse microglial...

journal_title：Biochemical and biophysical research communications

authors： Fukano Y,Okino N,Furuya S,Ito M

更新日期：2016-09-16 00:00:00

abstract：:The ubiquitous eukaryotic protein kinase CKII (casein kinase II) has been found to interact with a number of cellular proteins, either through the catalytic subunit or the regulatory subunit. Using the yeast two-hybrid screening method, we found that the catalytic subunit of Drosophila melanogaster CKII (DmCKII) inter...

journal_title：Biochemical and biophysical research communications

authors： Zhao W,Bidwai AP,Glover CV

更新日期：2002-10-18 00:00:00

abstract：:Interaction of P2X7 receptor with P2X4 receptor has recently been suggested, but it remains unclear whether P2X4 receptor is involved in P2X7 receptor-mediated events, such as cell death of macrophages induced by high concentrations of extracellular ATP. Here, we present evidence that P2X4 receptor does play a role in...

journal_title：Biochemical and biophysical research communications

authors： Kawano A,Tsukimoto M,Noguchi T,Hotta N,Harada H,Takenouchi T,Kitani H,Kojima S

更新日期：2012-03-09 00:00:00

abstract：:Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as...

journal_title：Biochemical and biophysical research communications

authors： Van De Parre TJ,Martinet W,Schrijvers DM,Herman AG,De Meyer GR

更新日期：2005-02-04 00:00:00

abstract：:Incubation of rabbit aortic endothelial cells (RAEC) with oxidized low-density lipoprotein (LDL) for 16 h resulted in stimulation of calcium uptake and increased release of nitric oxide (NO) by these cells. Accompanying inhibition of protein tyrosine phosphatase (PTP) activity in these cells was also observed. Convers...

journal_title：Biochemical and biophysical research communications

authors： Fries DM,Penha RG,D'Amico EA,Abdalla DS,Monteiro HP

更新日期：1995-02-06 00:00:00

abstract：:Fracture healing is a complicated regeneration process which to some extent recapitulates bone development. Fibroblast growth factor receptor 3 (FGFR3) has a negative regulatory effect on endochondral ossification, and FGFR3 is also expressed in prehypertrophic and hypertrophic chondrocytes during fracture healing. Ho...

journal_title：Biochemical and biophysical research communications

authors： Su N,Yang J,Xie Y,Du X,Lu X,Yin Z,Yin L,Qi H,Zhao L,Feng J,Chen L

更新日期：2008-11-21 00:00:00

abstract：:The effect of extracellular ATP on intracellular free Ca2+ was characterized in quin2-loaded parotid acinar cells. ATP specifically increased the intracellular Ca2+ concentration six-fold above a basal level of 180 nM. Of other purine nucleotides tested, only adenylylthiodiphosphate (ATP gamma S) had significant activ...

journal_title：Biochemical and biophysical research communications

authors： McMillian MK,Soltoff SP,Cantley LC,Talamo BR

更新日期：1987-12-16 00:00:00

abstract：:The marked impairment of hepatic drug metabolism during inflammation and infections has been known for many years and shown to result from down-regulation of cytochrome P450s (CYP) by cytokines. However, the mechanism of this repression is unknown. Using primary cultures of human hepatocytes, we show here that interle...

journal_title：Biochemical and biophysical research communications

authors： Pascussi JM,Gerbal-Chaloin S,Pichard-Garcia L,Daujat M,Fabre JM,Maurel P,Vilarem MJ

更新日期：2000-08-11 00:00:00

abstract：:Bovine aorta endothelial cells were cultured in medium containing [3H]glucosamine, [35S]sulfate, and various concentrations of chlorate. Cell growth was not affected by 10 mM chlorate, while 30 mM chlorate had a slight inhibitory effect. Chlorate concentrations greater than 10 mM resulted in significant undersulfation...

journal_title：Biochemical and biophysical research communications

authors： Humphries DE,Silbert JE

更新日期：1988-07-15 00:00:00

abstract：:This study aims to investigate the clinical significance and biological function of RASSF6 in human breast cancers. RASSF6 protein was found to be downregulated in 42 of 95 human breast cancer tissues by immunohistochemistry, which was associated with advanced TNM stage and nodal metastasis. The rate of RASSF6 downreg...

journal_title：Biochemical and biophysical research communications

authors： He Z,Zhao TT,Jin F,Li JG,Xu YY,Dong HT,Liu Q,Xing P,Zhu GL,Xu H,Miao ZF

更新日期：2018-09-18 00:00:00

abstract：:Angiotensin II (Ang II) and oxidized LDL (Ox-LDL) are risk factors for atherosclerosis, and both of them contribute to macrophage cholesterol accumulation, the hallmark of early atherosclerosis. As Ang II was shown to increase macrophage uptake of Ox-LDL, we investigated the effect of losartan, an Ang II receptor anta...

journal_title：Biochemical and biophysical research communications

authors： Hayek T,Aviram M,Heinrich R,Sakhnini E,Keidar S

更新日期：2000-07-05 00:00:00

abstract：:A soluble protein that binds malonyl-CoA without requiring cofactors has been purified from rat liver. Until saturated, it competes with fatty acid synthetase for free malonyl-CoA, temporarily reducing the rate of fatty acid synthesis at low levels of malonyl-CoA, as in fatty acid synthetase--coupled assays for acetyl...

journal_title：Biochemical and biophysical research communications

authors： Dugan RE,Osterlund BR,Drong RF,Swenson TL

更新日期：1987-08-31 00:00:00

abstract：:The present study was performed to determine if interleukin-1 (IL-1) acts as a chemical messenger in the central nervous system (CNS) regulation of gastric secretion and ulcer formation, using male Wistar rats. The central injection of IL-1 dose-dependently inhibited gastric acid secretion in pylorus-ligated rats at 1...

journal_title：Biochemical and biophysical research communications

authors： Uehara A,Okumura T,Kitamori S,Takasugi Y,Namiki M

更新日期：1990-12-14 00:00:00

abstract：:Triple negative breast cancer (TNBC) is the hardest breast cancer subtype to treat due to lacking therapeutic target and treatment options. In this study, we found that SLUG expression was much higher in TNBC MDA-MB-231 cells than estrogen receptor alpha (ERα) positive breast cancer MCF7 cells. 4-hydroxytamoxifen (4-O...

journal_title：Biochemical and biophysical research communications

authors： Geng C,Li J,Ding F,Wu G,Yang Q,Sun Y,Zhang Z,Dong T,Tian X

更新日期：2016-04-22 00:00:00

abstract：:Cell adhesion molecules act as tumor suppressors primarily by cell attachment activity, but additional mechanisms modifying signal transduction are suggested in some cases. Cell adhesion molecule 1 (CADM1), a membrane-spanning immunoglobulin superfamily, mediates intercellular adhesion by trans-homophilic interaction ...

journal_title：Biochemical and biophysical research communications

authors： Tsuboi Y,Oyama M,Kozuka-Hata H,Ito A,Matsubara D,Murakami Y

更新日期：2020-08-27 00:00:00

abstract：:H-rev107, which belongs to class II tumor suppressor genes, is ubiquitously expressed in normal cells, but is downregulated in many carcinomas and tumor cell lines. Sequence analysis showed that the murine H-rev107 gene is composed of five exons and four introns. Transfections revealed that 7.6 kb of the H-rev107 prom...

journal_title：Biochemical and biophysical research communications

authors： Roder K,Latasa MJ,Sul HS

更新日期：2002-05-03 00:00:00

abstract：:Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K(+)-ATPase (gastric proton pump). Immunoblotting experiments ind...

journal_title：Biochemical and biophysical research communications

authors： Wang XH,Miyazaki Y,Shinomura Y,Moriyama Y,Nakamoto RK,Matsuzawa Y,Maeda M,Futai M

更新日期：1993-01-15 00:00:00

abstract：:The hspA gene encodes a small heat-shock protein in the thermophilic cyanobacterium Synechococcus vulcanus. To gain insight into the post-transcriptional regulation, hspA was expressed in Escherichia coli. HspA was induced upon a temperature upshift from 30 to 42 degrees C, although the hspA transcription in E. coli o...

journal_title：Biochemical and biophysical research communications

authors： Kojima K,Nakamoto H

更新日期：2005-06-03 00:00:00

abstract：:Two novel allergens, designated Mal f 2 and Mal f 3 according to the WHO/IUIS Allergen Nomenclature Subcommittee recommendation, were isolated from the lipophilic yeast Malassezia furfur cell extracts and the genes coding for those were cloned. Mal f 2 and Mal f 3 had apparent molecular weights of 21 kDa and 20 kDa, r...

journal_title：Biochemical and biophysical research communications

authors： Yasueda H,Hashida-Okado T,Saito A,Uchida K,Kuroda M,Onishi Y,Takahashi K,Yamaguchi H,Takesako K,Akiyama K

更新日期：1998-07-20 00:00:00

abstract：:Histone deacetylase inhibitors (HDACIs) are promising anti-tumor agents that selectively induce cell cycle arrest, differentiation and/or apoptosis of tumor cells. Fundamentally, HDACIs are proposed to function by activating the transcription of genes, including the potent cyclin dependent kinase inhibitor p21(WAF1). ...

journal_title：Biochemical and biophysical research communications

authors： Hirsch CL,Ellis DJ,Bonham K

更新日期：2010-11-26 00:00:00

abstract：:Hexokinase II protein is augmented in denervated skeletal muscle; therefore, we determined if hexokinase II gene transcription rates and mRNA levels are increased with denervation. The right hindlimb skeletal muscles of male rats were denervated while the left hindlimbs were sham operated. Seventy-two h following surg...

journal_title：Biochemical and biophysical research communications

authors： Jones JP,Roberts BR,Tapscott EB,Dohm GL

更新日期：1997-09-08 00:00:00

abstract：:One of the large, unsolved problems in human genetics is the proportion of functional sequences in genomes. Recently, the encyclopedia of DNA elements consortium revealed that the majority of the genome is biochemically active, which were described as biochemical functions. This has been used as evidence to pronounce ...

journal_title：Biochemical and biophysical research communications

authors： Niu DK,Jiang L

更新日期：2013-01-25 00:00:00

abstract：:We report a 39 kDa substrate for cholera and pertussis toxins is present in D. discoideum membranes. This protein did not co-migrate with alpha subunits of either Gs (45 kDa and 52 kDa) or Gi (41 kDa) from control mammalian cells. The presence of GTP or its non-hydrolyzable analogs enhanced the ADP-ribosylation in res...

journal_title：Biochemical and biophysical research communications

authors： Khachatrian L,Klein C,Howlett A

更新日期：1987-12-31 00:00:00

abstract：:Auxins regulate various aspects of plant growth and development. However, the mechanism by which these hormones elicit diverse physiological processes is not clear. We present evidence for the role of auxin in protein phosphorylation and the possible involvement of calmodulin in auxin-induced changes. In the presence ...

journal_title：Biochemical and biophysical research communications

authors： Reddy AS,Chengappa S,Poovaiah BW

更新日期：1987-04-29 00:00:00