Cloning, expression, and characterization of a novel sialidase from Brevibacterium casei.

abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...

journal_title：Biotechnology and applied biochemistry

authors： Ramström M,Zuberovic A,Grönwall C,Hanrieder J,Bergquist J,Hober S

更新日期：2009-02-01 00:00:00

abstract：:A microbial fuel cell is a sustainable and environmental-friendly device that combines electricity generation and wastewater treatment through metabolic activities of microorganisms. However, low power output from inadequate electron transfer to the anode electrode hampers its practical implementation. Nanocomposites ...

journal_title：Biotechnology and applied biochemistry

authors： Sirajudeen AAO,Annuar MSM,Subramaniam R

更新日期：2020-04-21 00:00:00

abstract：:Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (E...

journal_title：Biotechnology and applied biochemistry

authors： Lau MF,Chua KH,Sabaratnam V,Kuppusamy UR

更新日期：2020-08-28 00:00:00

abstract：:The survival of inoculated microbes is critical for successful bioaugmentation in wastewater treatment. The influence of readily available nutrients (RANs) on the colonization of two functional bacteria, Pseudomonas putida M9, a strong biofilm-forming strain, and Comamonas testosteroni A3, a 3,5-dinitrobenzoic acid (3...

journal_title：Biotechnology and applied biochemistry

authors： Cheng Z,Meng X,Xie L,Xu H,Li M

更新日期：2015-09-01 00:00:00

abstract：:A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with platinum nanoparticles (Pt) and blocking reagent horseradish peroxidase (HRP) as enhancers. A Nafion®-graphene-coated electrode was first modified with an electrochemical probe of methylene blue (MB) through elect...

journal_title：Biotechnology and applied biochemistry

authors： Sun T,Wang L,Li N,Gan X

更新日期：2011-07-01 00:00:00

abstract：:The antifungal activity of iturin A and its interaction with erythrocyte membranes were enhanced in the presence of surfactin. The modification of the properties of iturin A was explained by the formation of mixed iturin A-surfactin micelles. Such mixed micelles were easily generated when both lipopeptides were in aqu...

journal_title：Biotechnology and applied biochemistry

authors： Thimon L,Peypoux F,Maget-Dana R,Roux B,Michel G

更新日期：1992-10-01 00:00:00

abstract：:Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrog...

journal_title：Biotechnology and applied biochemistry

authors： Chirila TV,Suzuki S,Papolla C

更新日期：2017-11-01 00:00:00

abstract：:Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in de...

journal_title：Biotechnology and applied biochemistry

authors： Chen L,Guo S,Wu L,Hao C,Xu W,Zhang J

更新日期：2015-05-01 00:00:00

abstract：:The effect of silica nanoparticles and conventional silica sources on the changes in microbial biomass and silica availability to pure soil and maize rhizosphere was studied. Nanosilica (20-40 nm) was synthesized from rice husk and comprehensively characterized. The efficiency of nanosilica was evaluated in terms of i...

journal_title：Biotechnology and applied biochemistry

authors： Rangaraj S,Gopalu K,Rathinam Y,Periasamy P,Venkatachalam R,Narayanasamy K

更新日期：2014-11-01 00:00:00

abstract：:Bovine serum albumin (BSA) microspheres with an average diameter of 12.5 micron were prepared by crosslinking of BSA molecules with glutaraldehyde in the presence of polymethylmethacrylate dissolved in chloroform-toluene. Trypsin and anti-human IgG antibody were immobilized onto their surfaces by the glutaraldehyde-ac...

journal_title：Biotechnology and applied biochemistry

authors： Yan CH,Kitano H,Ise N

更新日期：1988-02-01 00:00:00

abstract：:Mechano-growth factor (MGF), an alternative splicing variant of insulin-like growth factor-1 (IGF-1) gene, promotes cell proliferation and inhibits cell differentiation. It also plays an important role in tumor development. It is important to optimize the production process and achieve MGF protein because there is no ...

journal_title：Biotechnology and applied biochemistry

authors： Feng J,Wan R,Yi Q,He L,Yang L,Tang L

更新日期：2015-09-01 00:00:00

abstract：:Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for bo...

journal_title：Biotechnology and applied biochemistry

authors： Parascandola P,de Alteriis E

更新日期：1996-02-01 00:00:00

abstract：:Metal prostheses of artificial joints undergo wear, producing numerous metal particles and ions, such as Cr3+ . Cr3+ is considered a key factor leading to aseptic loosening. Many studies focus on the effect of Cr3+ on osteoblasts; however, little is known about the effect of Cr3+ on the B-cell maturation antigen (BCMA...

journal_title：Biotechnology and applied biochemistry

authors： Li DH,Yang Q,Zhou JS,Zhang ZW,Miao MY,Yang SS,Xu WD

更新日期：2017-09-01 00:00:00

abstract：:Glutamate dehydrogenase (GDH) and alcohol dehydrogenase (ADH) have been encapsulated in sheep and human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At a fixed enzyme level in the dialysis bag (100 units/ml of RBCs), a significant encapsulation yield was observed for ADH, both in human ...

journal_title：Biotechnology and applied biochemistry

authors： Sanz S,Pinilla M,Garín M,Tipton KF,Luque J

更新日期：1995-10-01 00:00:00

abstract：:Bioreactor process changes can have a profound effect on the yield and quality of biotechnology products. Mannose-6-phosphate (M6P) glycan content and the enzymatic catalytic kinetic parameters are critical quality attributes (CQAs) of many therapeutic enzymes used to treat lysosomal storage diseases (LSDs). Here, we ...

journal_title：Biotechnology and applied biochemistry

authors： Madhavarao CN,Agarabi CD,Wong L,Müller-Loennies S,Braulke T,Khan M,Anderson H,Johnson GR

更新日期：2014-03-01 00:00:00

abstract：:Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, the molecular cause of the disease is poorly elucidated. In the present study, we primarily found that translationally controlled tumor protein (TCTP) was overexpressed in human OS tissues and cell lines. To investigate t...

journal_title：Biotechnology and applied biochemistry

authors： Shen JH,Qu CB,Chu HK,Cui MY,Wang YL,Sun YX,Song YD,Li G,Shi FJ

更新日期：2016-01-01 00:00:00

abstract：:Restoration of insulin secretion by transplantation of isolated islets is a treatment for type Ι diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate e...

journal_title：Biotechnology and applied biochemistry

authors： Li N,Zhang Y,Xiu Z,Wang Y,Chen L,Wang S,Li S,Guo X,Ma X

更新日期：2015-07-01 00:00:00

abstract：:Some metabolic inhibitors, mevastatin (MVS), chlorocholine chloride (CCC), sodium pyrophosphate (NaPP) and D,L-glyceraldehyde (DLG), were respectively added into the suspension cultures of the yew Taxus chinensis var. mairei at the late phase of cell growth to study isopentenyl pyrophosphate (IPP) biosynthesis. The co...

journal_title：Biotechnology and applied biochemistry

authors： Wang YD,Yuan YJ,Lu M,Wu JC,Jiang JL

更新日期：2003-02-01 00:00:00

abstract：:The α-amylase from soya bean seeds was purified by affinity precipitation, resulting in approx. 20-fold purification with approx. 84% recovery. The purified α-amylase had an optimum pH of 5.5, optimum temperature of 75 °C, Arrhenius energy of activation of 6.03 kcal/mol (1 kcal≈4.184 kJ) and a Km of 2.427 mg/ml (starc...

journal_title：Biotechnology and applied biochemistry

authors： Prakash O,Jaiswal N

更新日期：2010-12-01 00:00:00

abstract：:Human urokinase was immobilized on an ethylene vinyl acetate copolymer surface. Soluble urokinase showed its maximum activity at pH 8.5, while the immobilized enzyme was most active at pH 9.0. Apparently, the shift in optimal pH was due to the polyanionic nature of the carrier surface on which the enzyme was immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Yabushita Y

更新日期：1988-06-01 00:00:00

abstract：:A simple and cost-effective method is described to sequence double-stranded DNA (< 100 ng) using a very small amount of fluorescent sequencing mix (one-tenth of manufacturer-recommended amount). The modification has significant advantages over conventional protocols and can be used to sequence, using a single sequenci...

journal_title：Biotechnology and applied biochemistry

authors： Beg OU,Holt RG

更新日期：1997-08-01 00:00:00

abstract：:Aspergillus niger and Rhizopus sp. glucoamylases were purified on an affinity chromatography column from commercially available, impure enzyme preparations. Up to 2 mg of glucoamylase protein was bound without leakage to a 1-ml affinity gel column (0.7 X 2.5 cm) possessing a covalently linked acarbose ligand (1 mg aca...

journal_title：Biotechnology and applied biochemistry

authors： Ono K,Smith EE

更新日期：1986-04-01 00:00:00

abstract：:The variation with pH of the kinetic parameters of penicillin acylase from Kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. The pH-dependence of log (V/Km) for penicillin G showed that a group with a pK value over 4.7 must be deprotonated and t...

journal_title：Biotechnology and applied biochemistry

authors： Martín J,Prieto I,Mancheño JM,Barbero JL,Arche R

更新日期：1993-06-01 00:00:00

abstract：:Sephadex was derivatized consecutively with Staphylococcus Protein A (SpA) and cell-specific antibodies, and the binding of cells to the resulting material was examined. For comparison, cell binding to commercially obtained SpA-Sepharose was determined. Sephadex G-10, carboxylated by reaction with glycine and activate...

journal_title：Biotechnology and applied biochemistry

authors： Besselink G,de Korte D

更新日期：2002-02-01 00:00:00

abstract：:Two different enzymes that metabolize lactate in the presence of oxygen, either to acetate plus CO2 (lactate 2-mono-oxygenase; Lmox) or to pyruvate plus H2O2 (lactate oxidase; Lox) were encapsulated in human and murine red blood cells (RBCs). Lmox shows a low affinity for lactate (Km 22 mM) and thus works at a low rat...

journal_title：Biotechnology and applied biochemistry

authors： Garin M,Rossi L,Luque J,Magnani M

更新日期：1995-12-01 00:00:00

abstract：:Cholesterol esterase modified with polyethylene glycol was able to dissolve in some highly hydrophobic solvents such as benzene and toluene, and catalyze the synthesis of cholesterol linoleate with time dependency in the reverse of the usual reaction in aqueous solvents. Enzymatic cholesterol linoleate synthesis follo...

journal_title：Biotechnology and applied biochemistry

authors： Mori S,Nakata Y,Endo H

更新日期：1992-06-01 00:00:00

abstract：:The structural underpinnings of O₂ activation in P450s have been limited by the inability to trap unstable oxy complexes in the crystalline state for structure determination. To date there are only two known oxy P450 structures. Even so, much is known about O₂ activation in P450cam and the role that redox partner bind...

journal_title：Biotechnology and applied biochemistry

authors： Poulos TL,Madrona Y

更新日期：2013-01-01 00:00:00

abstract：:The protective antigen (PA) component of anthrax toxin mediates delivery of either lethal factor (LF) or oedema factor into the cytosol of mammalian cells. The N-terminal domain of LF(1-254) (amino acids 1-254 of LF) binds to PA and, when fused to heterologous proteins, delivers such proteins into the cytosol. In the ...

journal_title：Biotechnology and applied biochemistry

authors： Sharma M,Khanna H,Arora N,Singh Y

更新日期：2000-08-01 00:00:00

abstract：:Fetuin is a plasma glycoprotein widely distributed in mammals. It has been used as a model protein for structural analyses and investigations into the biological properties of glycoproteins. A convenient one-step procedure for biospecific isolation of fetuin from fetal bovine serum was developed on the basis of wheatg...

journal_title：Biotechnology and applied biochemistry

authors： Cartellieri S,Hamer O,Helmholz H,Niemeyer B

更新日期：2002-04-01 00:00:00

abstract：:Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin inj...

journal_title：Biotechnology and applied biochemistry

authors： Calcabrini C,Mancini U,De Bellis R,Diaz AR,Martinelli M,Cucchiarini L,Sestili P,Stocchi V,Potenza L

更新日期：2017-05-01 00:00:00