Studies on the properties of immobilized urokinase: effects of pH and temperature.

abstract：:Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an au...

journal_title：Biotechnology and applied biochemistry

authors： Jantra J,Zór K,Sanders M,De Saeger S,Hedström M,Mattiasson B

更新日期：2020-05-01 00:00:00

abstract：:The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently, we reported that a de novo carbene transferase, a tetra-α-helical c-type heme-containing protein, C45, is proficient at N-H insertion reactions, proceeding via the intermolecular transfer of a metallocarben...

journal_title：Biotechnology and applied biochemistry

authors： Stenner R,Anderson JLR

更新日期：2020-07-01 00:00:00

abstract：:Expression of the VP2 gene of autonomous parvoviruses in insect cells with the use of the baculovirus system has led to the production of virus-like particles (VLPs) formed by the self-assembly of VP2. These VLPs are expressed at high levels and can easily be purified by salt fractionation. They are highly immunogenic...

journal_title：Biotechnology and applied biochemistry

authors： Casal JI

更新日期：1999-04-01 00:00:00

abstract：:Human thymosin alpha1 is an effective immune system enhancer for the treatment of cancer and viral diseases. Therefore the development of new methods for its synthesis is an urgent problem. In the present work, we propose an efficient scalable scheme for the production of recombinant thymosin alpha1. We used an expres...

journal_title：Biotechnology and applied biochemistry

authors： Esipov RS,Stepanenko VN,Beyrakhova KA,Muravjeva TI,Miroshnikov AI

更新日期：2010-05-19 00:00:00

abstract：:The application of the sulfhydryl protease ficin as biocatalyst is proposed as a novel method for enzyme-catalyzed synthesis of dipeptides. The negligible peptidase but considerable esterase activity at alkaline pH facilitated the kinetically controlled formation of peptide bonds by coupling the ester substrates Z-Ala...

journal_title：Biotechnology and applied biochemistry

authors： Monter B,Herzog B,Stehle P,Fürst P

更新日期：1991-10-01 00:00:00

abstract：:The aim of this paper is to make the point on the fortieth years study on the β-glycosidase from Sulfolobus solfataricus. This enzyme represents one of the thermophilic biocatalysts, which is more extensively studied as witnessed by the numerous literature reports available since 1980. Comprehensive biochemical studie...

journal_title：Biotechnology and applied biochemistry

authors： Febbraio F,Ionata E,Marcolongo L

更新日期：2020-07-01 00:00:00

abstract：:This study was designed to investigate the protective effects of Acanthus ilicifolius alkaloid A [4-hydroxy-2-benzoxazolone (HBOA)] and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride (CCl4 )-induced liver fibrosis in rats. Spr...

journal_title：Biotechnology and applied biochemistry

authors： Wai KK,Liang Y,Zhou L,Cai L,Liang C,Liu L,Lin X,Wu H,Lin J

更新日期：2015-07-01 00:00:00

abstract：:Carbamate kinase has been prepared from Lactobacillus buchneri NCDO110. An approximately 91-fold increase in the specific activity of the enzyme was achieved. The purified extract exhibited a single band following polyacrylamide gel electrophoresis. The apparent molecular weight as determined by gel electrophoresis wa...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Nadra Chaud CA,Pesce de Ruiz Holgado A,Oliver G

更新日期：1986-02-01 00:00:00

abstract：:Nicotine is a potent parasympathomimetic stimulant and an important natural alkaloid mainly found in the Nicotiana genus of plants. It can directly threaten ecological security and human health in tobacco waste, wastewater, and other forms of tobacco production. Therefore, it is the basis of nicotine pollution prevent...

journal_title：Biotechnology and applied biochemistry

authors： Ruan A,Gao Y,Fang C,Xu Y

更新日期：2018-11-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of garlic (Allium sativum L.) on the diabetic nephropathy and oxidative stress induced by STZ (streptozotocin) in rats. Diabetes was induced in Male Sprague-Dawley rats by administering a single intraperitoneal injection of STZ (60 mg/kg of body weight). ...

journal_title：Biotechnology and applied biochemistry

authors： Mariee AD,Abd-Allah GM,El-Yamany MF

更新日期：2009-03-01 00:00:00

abstract：:Cholesterol esterase modified with polyethylene glycol was able to dissolve in some highly hydrophobic solvents such as benzene and toluene, and catalyze the synthesis of cholesterol linoleate with time dependency in the reverse of the usual reaction in aqueous solvents. Enzymatic cholesterol linoleate synthesis follo...

journal_title：Biotechnology and applied biochemistry

authors： Mori S,Nakata Y,Endo H

更新日期：1992-06-01 00:00:00

abstract：:The dynamic gassing-out method was used to assess the effect of stirrer speed, air flow rate and alginate concentration on the overall volumetric oxygen mass transfer coefficient (K(L)a) in a bench-top stirred fermenter equipped with two Rushton-type turbines. As the alginate concentration in the medium was increased ...

journal_title：Biotechnology and applied biochemistry

authors： Parente E,Piraino P,Fidaleo M,Moresi M

更新日期：2004-10-01 00:00:00

abstract：:An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferul...

journal_title：Biotechnology and applied biochemistry

authors： Kroon PA,Faulds CB,Williamson G

更新日期：1996-06-01 00:00:00

abstract：:Fetuin is a plasma glycoprotein widely distributed in mammals. It has been used as a model protein for structural analyses and investigations into the biological properties of glycoproteins. A convenient one-step procedure for biospecific isolation of fetuin from fetal bovine serum was developed on the basis of wheatg...

journal_title：Biotechnology and applied biochemistry

authors： Cartellieri S,Hamer O,Helmholz H,Niemeyer B

更新日期：2002-04-01 00:00:00

abstract：:The participation of Mg2+ or Mn2+ nucleoside diphosphates in the reverse reaction catalyzed by purified carbamate kinase (ATP:carbamate phosphotransferase, EC 2.7.2.2) of Lactobacillus buchneri NCDO110 was studied. The results of initial velocity studies have indicated that Mn2+ ADP is as effective as a substrate as M...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Pesce de Ruiz Holgado AA,Oliver G

更新日期：1987-04-01 00:00:00

abstract：:The production of rabies virus from baby hamster kidney-Ankara66 (BHK-An(66), HUKUK 99050302) monolayer cells was examinedin a packed-bed reactor containing non-woven polyester fabric (NWPF)discs. At first, growth characteristics of the cells were determined instatic culture. The suspension culture studies were realiz...

journal_title：Biotechnology and applied biochemistry

authors： Gümüşderelioğlu M,Aslankaraoğlu E,Gürhan SI

更新日期：2001-06-01 00:00:00

abstract：:Higher thermostability or acid resistance for fungal α-amylase will help to improve the sugar-making process and cut down the production costs. Here, the thermostability or acid resistance of Rhizopus oryzae α-amylase (ROAmy) was significantly enhanced by site-directed evolution based on multiple sequence alignment (M...

journal_title：Biotechnology and applied biochemistry

authors： Li S,Yang Q,Tang B

更新日期：2020-07-01 00:00:00

abstract：:Biological mineralization is a highly programmed process in which inorganic minerals reassociate under the strict control of the extracellular matrix to form minerals with special functions and patterns. Shells are biominerals, and their synthesis is finely regulated by organic matrix including matrix proteins, polysa...

journal_title：Biotechnology and applied biochemistry

authors： Jin C,Ren HY,Pu JW,Liu XJ,Li JL

更新日期：2019-07-01 00:00:00

abstract：:Catalytic properties of Escherichia coli polyphosphate kinase (EC 2.7.4.1), a promising enzyme for use in ATP regeneration (Hoffman, et al., 1988, Biotechnol. Appl. Biochem. 10, 107-117), are reported here. E. coli polyphosphate kinase (PPK) is broadly active in the pH range 5.5 to 8.5, having an optimal Vmax at pH 7....

journal_title：Biotechnology and applied biochemistry

authors： Haeusler PA,Dieter L,Rittle KJ,Shepler LS,Paszkowski AL,Moe OA

更新日期：1992-04-01 00:00:00

abstract：:The development of lytic bacteriophages as therapeutic products is an attractive alternative to antibiotics. In this study, we evaluated the potential of phage tails for lysing Gram-positive bacteria. Phage P954, a well-characterized temperate staphylococcal phage, was found to adsorb to a large number of Staphylococc...

journal_title：Biotechnology and applied biochemistry

authors： Biddappa AC,Sundarrajan S,Ramalinga AB,Sriram B,Padmanabhan S

更新日期：2012-11-01 00:00:00

abstract：:Melamine adulteration of food is a public health concern. It has been seen that melamine causes disease in many organs. Melamine-induced kidney disease is a well-recognized clinicopathological entity. Inflammation is thought to be important in melamine-induced pathology. Melamine is expected to bind with albumin becau...

journal_title：Biotechnology and applied biochemistry

authors： Rajpoot M,Bhattacharyya R,Banerjee D,Sharma A

更新日期：2017-07-01 00:00:00

abstract：:The dynamics of dissolved oxygen (DO) and shear stress in the vasculature microenvironment play a major role in determining the fate of stem cells in adults and during early embryonic development. In this study, we present a microbioreactor (MBR) that provides independent control over oxygen tension and shear stress i...

journal_title：Biotechnology and applied biochemistry

authors： Abaci HE,Devendra R,Soman R,Drazer G,Gerecht S

更新日期：2012-03-01 00:00:00

abstract：:Bioreactor process changes can have a profound effect on the yield and quality of biotechnology products. Mannose-6-phosphate (M6P) glycan content and the enzymatic catalytic kinetic parameters are critical quality attributes (CQAs) of many therapeutic enzymes used to treat lysosomal storage diseases (LSDs). Here, we ...

journal_title：Biotechnology and applied biochemistry

authors： Madhavarao CN,Agarabi CD,Wong L,Müller-Loennies S,Braulke T,Khan M,Anderson H,Johnson GR

更新日期：2014-03-01 00:00:00

abstract：:Glutamate dehydrogenase (GDH) and alcohol dehydrogenase (ADH) have been encapsulated in sheep and human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At a fixed enzyme level in the dialysis bag (100 units/ml of RBCs), a significant encapsulation yield was observed for ADH, both in human ...

journal_title：Biotechnology and applied biochemistry

authors： Sanz S,Pinilla M,Garín M,Tipton KF,Luque J

更新日期：1995-10-01 00:00:00

abstract：:The combination of IL-2 (interleukin-2) and GM-CSF (granulocyte/macrophage colony-stimulating factor) has been broadly studied in antitumour immune therapy, but its efficacy is uncertain. To better exert the activities of the two cytokines and study them in a mouse model, we have constructed a bifunctional protein, hI...

journal_title：Biotechnology and applied biochemistry

authors： Wen Q,Ma L,Luo W,Zhou MQ,He D,Lin Y,Wu ZQ,He XW,Wang JF,Wang XN

更新日期：2008-05-01 00:00:00

abstract：:Second-generation bioethanol production process was developed using pretreated empty fruit bunches (EFB). Consecutive acid/alkali EFB pretreatment was performed, first with HCl and then with NaOH with final washing steps for phenolic compounds elimination. Scanning electron microscopy images showed that EFB chemical t...

journal_title：Biotechnology and applied biochemistry

authors： Campioni TS,Soccol CR,Libardi Junior N,Rodrigues C,Woiciechowski AL,Letti LAJ,Vandenberghe LPS

更新日期：2020-09-01 00:00:00

abstract：:This paper is concerned with the optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Auricularia polytricha by one-factor-at-a-time and uniform design (UD) methods. First, the one-factor-at-a-time method was adopted to investigate the effects of environmental factors (i.e....

journal_title：Biotechnology and applied biochemistry

authors： Xu CP,Yun JW

更新日期：2003-10-01 00:00:00

abstract：:The use of the recombinant BoNT/A-Hc (carboxylic domain of the Clostridium botulinum neurotoxin heavy chain) has been proposed as a vaccine candidate for botulism. This fragment contains the principle protective antigenic determinant. In the present study, in order to maximize recombinant protein expression, after ver...

journal_title：Biotechnology and applied biochemistry

authors： Yari K,Fatemi SS,Tavallaei M

更新日期：2010-05-24 00:00:00

abstract：:The present study was aimed to explore the molecular and structural features of UDP-N-acetylglucosamine pyrophosphorylase of Bombyx mori (BmUAP), an essential enzyme for chitin synthesis in insects. The BmUAP cDNA sequence was cloned and expression profiles were monitored during the molting and feeding stages of silkw...

journal_title：Biotechnology and applied biochemistry

authors： Palaka BK,Velmurugan Ilavarasi A,Sapam TD,Kotapati KV,Nallala VS,Khan MB,Ampasala DR

更新日期：2019-09-01 00:00:00

abstract：:The immobilization of F(0)F(1)-ATPase in uniform orientation is reported. The biotinylated and histidine-tagged subunits of the bacterial F(0)F(1)-ATPase complex were used for immobilization of the complex on artificial semi-permeable membranes resulting in 88+/-7.8 and 72+/-5.2% coupling of the enzymes. The immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2004-06-01 00:00:00