Improving the thermostability and acid resistance of Rhizopus oryzae α-amylase by using multiple sequence alignment based site-directed mutagenesis.

abstract：:The plant polymer lignin is the greatest source of aromatic chemical structures on earth. Hence, the chemically diverse lignin monomers are valuable raw materials for fine chemicals, materials synthesis, and food and flavor industries. However, extensive use of this natural resource is hampered by the large number of ...

journal_title：Biotechnology and applied biochemistry

authors： Jansen F,Gillessen B,Mueller F,Commandeur U,Fischer R,Kreuzaler F

更新日期：2014-11-01 00:00:00

abstract：:High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...

journal_title：Biotechnology and applied biochemistry

authors： Eriksson C,Schwenk JM,Sjöberg A,Hober S

更新日期：2010-06-09 00:00:00

abstract：:Mouse band-3 crosslinked carrier erythrocytes have been prepared. [125I]Carbonic anhydrase (CA) has been encapsulated into mouse erythrocytes. Then, loaded erythrocytes were labelled with 51Cr. Eventually, these doubly labelled cells were crosslinked with band-3 crosslinking reagents. [125I]CA was shown to have cytoso...

journal_title：Biotechnology and applied biochemistry

authors： Jordán JA,Alvarez FJ,Tejedor MC,Díez JC

更新日期：1999-02-01 00:00:00

abstract：:Metal prostheses of artificial joints undergo wear, producing numerous metal particles and ions, such as Cr3+ . Cr3+ is considered a key factor leading to aseptic loosening. Many studies focus on the effect of Cr3+ on osteoblasts; however, little is known about the effect of Cr3+ on the B-cell maturation antigen (BCMA...

journal_title：Biotechnology and applied biochemistry

authors： Li DH,Yang Q,Zhou JS,Zhang ZW,Miao MY,Yang SS,Xu WD

更新日期：2017-09-01 00:00:00

abstract：:Feeding a basal diet free of vitamins E and C to weanling male rats for 8 months resulted in biochemical changes characteristic of vitamin E deficiency. These included increased liver thiobarbituric acid values; decreased blood GSH levels, plasma vitamin E levels, and glutathione peroxidase activities; and increased a...

journal_title：Biotechnology and applied biochemistry

authors： Chen LH,Thacker RR

更新日期：1986-02-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs), the largest family of cell-surface molecules involved in a number of biological and pathological processes, have recently emerged as key players in carcinogenesis and cancer progression. Orphan G protein-coupled receptors (oGPCRs) are a group of proteins lacking endogenous ligands....

journal_title：Biotechnology and applied biochemistry

authors： Cui X,Liu Y,Wang B,Xian G,Liu X,Tian X,Qin C

更新日期：2015-11-01 00:00:00

abstract：:Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by bacterial fermentation and chemical and enzymatic processing is currently under development. This stu...

journal_title：Biotechnology and applied biochemistry

authors： Suwan J,Torelli A,Onishi A,Dordick JS,Linhardt RJ

更新日期：2012-11-01 00:00:00

abstract：:A truncated precursor form of prostate-specific antigen (PSA), [-2]proPSA, is a well-known biomarker for prostate cancer. To develop a biomarker assay, highly purified [-2]proPSA is required as a standard reference and for generation of a specific antibody. In this study, we generated an efficient mammalian expression...

journal_title：Biotechnology and applied biochemistry

authors： Hwang D,Yoon A,Kim S,Kim H,Chung J

更新日期：2017-05-01 00:00:00

abstract：:The development of subunit vaccines is presently the main strategy being evaluated for prevention of infectious diseases. The use of recombinant-DNA techniques has facilitated the development of new principles for design and production of subunit vaccines. First of all, the properties of a target protein immunogen can...

journal_title：Biotechnology and applied biochemistry

authors： Hansson M,Nygren PA,Ståhl S

更新日期：2000-10-01 00:00:00

abstract：:Lipases are surface-active enzymes, acting on their substrates at the polar/nonpolar interface in emulsions. This study was aimed to test whether their activity, specificity, and the rates of formation/degradation of the various hydrolysis intermediates (i.e., mono- and diglycerides of interest as surface-active agent...

journal_title：Biotechnology and applied biochemistry

authors： Carpen A,Bonomi F,Iametti S,Marengo M

更新日期：2019-07-01 00:00:00

abstract：:The interaction of four lectins from crops of the legume family with Saccharomyces cerevisiae alpha-mannan, and also with two glycoenzymes containing mainly alpha-mannan moieties, has been studied. The interaction was characterized by a quantitative precipitation assay. The results of precipitation differ with respect...

journal_title：Biotechnology and applied biochemistry

authors： Mislovicová D,Gemeiner P,Sandula J,Masárová J,Vikartovská A,Docolomanský P

更新日期：2000-04-01 00:00:00

abstract：:The phosphatase and tensin homolog (PTEN) gene plays a crucial role in signal transduction by negatively regulating the PI3K signaling pathway. It is the most frequent mutated gene in many human-related cancers. Considering its critical role, a functional analysis of missense mutations of PTEN gene was undertaken in t...

journal_title：Biotechnology and applied biochemistry

authors： Khan I,Ansari IA,Singh P,Dass J FP

更新日期：2017-09-01 00:00:00

abstract：:Synthetic membranes containing 10% acrylamide units were subjected to activation with formaldehyde at pH 7.5 and 45 degrees C. Trypsin, invertase, and urease were bound to this activated membrane and the kinetic properties of immobilized enzymes were studied. The permeability of the membrane for distilled water manife...

journal_title：Biotechnology and applied biochemistry

authors： Krysteva MA,Shopova BI,Yotova LY,Karasavova MI

更新日期：1991-02-01 00:00:00

abstract：:hES (human embryonic stem) cells hold tremendous potential in the newly emerging field of regenerative medicine, in addition to being a useful tool in basic scientific research and for pharmacological and cytotoxicity screening. However, an essential prerequisite for the future widespread application of hES cells are ...

journal_title：Biotechnology and applied biochemistry

authors： Heng BC,Kuleshova LL,Bested SM,Liu H,Cao T

更新日期：2005-04-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...

journal_title：Biotechnology and applied biochemistry

authors： Ramström M,Zuberovic A,Grönwall C,Hanrieder J,Bergquist J,Hober S

更新日期：2009-02-01 00:00:00

abstract：:To reduce the degradation pathway of rhIFN-gamma (recombinant human interferon-gamma), the susceptibility against oxidative stress during fermentation in Escherichia coli cells and in purification process were investigated. Fermentations of recombinant E. coli were performed at 5, 30 and 60% DO (dissolved oxygen) conc...

journal_title：Biotechnology and applied biochemistry

authors： Mohammadian-Mosaabadi J,Naderi-Manesh H,Maghsoudi N,Khalilzadeh R,Shojaosadati SA,Ebrahimi M

更新日期：2005-02-01 00:00:00

abstract：:Aspergillus niger and Rhizopus sp. glucoamylases were purified on an affinity chromatography column from commercially available, impure enzyme preparations. Up to 2 mg of glucoamylase protein was bound without leakage to a 1-ml affinity gel column (0.7 X 2.5 cm) possessing a covalently linked acarbose ligand (1 mg aca...

journal_title：Biotechnology and applied biochemistry

authors： Ono K,Smith EE

更新日期：1986-04-01 00:00:00

abstract：:Lys and Leu were generally considered as the key amino acids for brewer's yeast during beer brewing. In the present study, peptide Lys-Leu and a free amino acid (FAA) mixture of Lys and Leu (Lys + Leu) were supplemented in 24 °P wort to examine their effects on physiological activity and fermentation performance of br...

journal_title：Biotechnology and applied biochemistry

authors： Yang H,Zong X,Cui C,Mu L,Zhao H

更新日期：2018-07-01 00:00:00

abstract：:In the present study, the effect of the neuroleptics chlorpromazine (2-chloro-N,N-dimethyl-10H-phenothiazine-10-propanamine) and trifluoperazine {10-[3-(4-methylpiperazin-l-yl)-propyl]-2-trifluoromethyl)-10-H- [phenothiazine}-10H-phenothiazine¿ on the EPR-spectroscopic properties of ferrous human adult nitrosylated ha...

journal_title：Biotechnology and applied biochemistry

authors： Ascenzi P,Bertollini A,Coletta M,Lucacchini A

更新日期：1999-10-01 00:00:00

abstract：:A simple and cost-effective method is described to sequence double-stranded DNA (< 100 ng) using a very small amount of fluorescent sequencing mix (one-tenth of manufacturer-recommended amount). The modification has significant advantages over conventional protocols and can be used to sequence, using a single sequenci...

journal_title：Biotechnology and applied biochemistry

authors： Beg OU,Holt RG

更新日期：1997-08-01 00:00:00

abstract：:Entrapped cells and entrapped cells grown inside of a calcium alginate matrix as well as free cells have been investigated using Saccharomyces cerevisiae mutant strains with regard to their pattern of growth and invertase activity. The repression of invertase by glucose and glucose-consumption ability were considered ...

journal_title：Biotechnology and applied biochemistry

authors： Rossi-Alva JC,Rocha-Leão MH

更新日期：2003-08-01 00:00:00

abstract：:The objective of this work is to develop structured, segregated stochastic models for bioprocesses using time-series flow cytometric (FC) data. To this end, mammalian CHO cells were grown in both batch and fed-batch cultures, and their viable cell numbers (VCDs), monoclonal antibody (MAb), cell cycle phases, mitochond...

journal_title：Biotechnology and applied biochemistry

authors： Shirsat N,Avesh M,English NJ,Glennon B,Al-Rubeai M

更新日期：2013-09-01 00:00:00

abstract：:The human placenta is a rich raw material for production of many biopharmaceutical products. Here we describe a co-purification process for the production of four different proteins from haemolysed human placenta blood: IgG, catalase (Cat), superoxide dismutase (Sod) and albumin (Alb). The process can be divided in tw...

journal_title：Biotechnology and applied biochemistry

authors： Grellet S,Martins EA,Maimoni Gonçalves V,Yague Lopes AP,Raw I,Cabrera-Crespo J

更新日期：2001-12-01 00:00:00

abstract：:Flat panels are the most spread type of photobioreactors for studying light effects on a microalgae culture. Their low thickness, usually between 1 and 3 cm, aims at ensuring light homogeneity across the culture. Yet because optical density has to remain very low, studies are still limited to low cell density cultures...

journal_title：Biotechnology and applied biochemistry

authors： Jiang W,Levasseur W,Casalinho J,Martin T,Puel F,Perré P,Pozzobon V

更新日期：2020-02-03 00:00:00

abstract：:The production of rabies virus from baby hamster kidney-Ankara66 (BHK-An(66), HUKUK 99050302) monolayer cells was examinedin a packed-bed reactor containing non-woven polyester fabric (NWPF)discs. At first, growth characteristics of the cells were determined instatic culture. The suspension culture studies were realiz...

journal_title：Biotechnology and applied biochemistry

authors： Gümüşderelioğlu M,Aslankaraoğlu E,Gürhan SI

更新日期：2001-06-01 00:00:00

abstract：:This study was designed to investigate the protective effects of Acanthus ilicifolius alkaloid A [4-hydroxy-2-benzoxazolone (HBOA)] and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride (CCl4 )-induced liver fibrosis in rats. Spr...

journal_title：Biotechnology and applied biochemistry

authors： Wai KK,Liang Y,Zhou L,Cai L,Liang C,Liu L,Lin X,Wu H,Lin J

更新日期：2015-07-01 00:00:00

abstract：:We have constructed a hybrid protein (ATFHI) consisting of an N-terminal fragment from urokinase (ATF) and HI-8, which is the C-terminal domain of urinary trypsin inhibitor. The fusion genes for the hybrid proteins were engineered by PCR and cloned into expression plasmids. Under the control of the tac promoter, fusio...

journal_title：Biotechnology and applied biochemistry

authors： Sugino D,Okushima M,Kobayashi H,Terao T

更新日期：1998-04-01 00:00:00

abstract：:The hybridization of the native and chemically inactivated aspartase from Escherichia coli was studied. Preparations of the tetrameric enzyme obtained by mixing the native and N-ethylmaleimide (NEM)-inactivated aspartase in 4 M guanidine-HCl followed by 51-fold dilution at room temperature retained catalytic activity....

journal_title：Biotechnology and applied biochemistry

authors： Imaishi H,Yumoto N,Tokushige M

更新日期：1990-04-01 00:00:00

abstract：:Naproxen and bezafibrate fall into the category of pharmaceuticals that have been widely detected in the aquatic environment, and one of the major sources is the effluent discharge from wastewater treatment plants. This study investigated the sorption and degradation kinetics of naproxen and bezafibrate in the presenc...

journal_title：Biotechnology and applied biochemistry

authors： Tang Y,Li XM,Xu ZC,Guo QW,Hong CY,Bing YX

更新日期：2014-05-01 00:00:00