Abstract：
:Aspergillus niger and Rhizopus sp. glucoamylases were purified on an affinity chromatography column from commercially available, impure enzyme preparations. Up to 2 mg of glucoamylase protein was bound without leakage to a 1-ml affinity gel column (0.7 X 2.5 cm) possessing a covalently linked acarbose ligand (1 mg acarbose/g wet gel), and the bound enzyme was specifically released by irrigation of the column with a solution of maltose. A complete cycle of purification was accomplished in about 8 h. Glucoamylases were recovered, in more than 80% yield, free of alpha-amylase activity and possessing specific activities comparable to those of preparations obtained by time-consuming, multistep procedures involving several ion-exchange and hydrophobic column fractionations. Thus, acarbose affinity chromatography provides a general method for the rapid and efficient purification of the glucoamylases, and seems to be ideally suited for scale-up for the commercial purification of these enzymes.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Ono K,Smith EEsubject
Has Abstractpub_date
1986-04-01 00:00:00pages
201-9issue
2-3eissn
0885-4513issn
1470-8744journal_volume
8pub_type
杂志文章abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20080015
更新日期：2009-02-01 00:00:00
abstract：:Human thymosin alpha1 is an effective immune system enhancer for the treatment of cancer and viral diseases. Therefore the development of new methods for its synthesis is an urgent problem. In the present work, we propose an efficient scalable scheme for the production of recombinant thymosin alpha1. We used an expres...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20100027
更新日期：2010-05-19 00:00:00
abstract：:Solubilization of 2',3',4',5'-tetrabenzoyl-5-acetyl-1,5-dihydroriboflavin (benzaflavin) by conjugates of BSA with poly(alkylene oxide)s [poly(ethylene glycol) and block co-polymers of ethylene oxide and propylene oxide (pluronics)] was investigated. Conjugates of BSA with pluronics were shown to have more solubilizing...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:alpha-Cyclodextrin (CD) was converted by Bacillus macerans cyclodextrin glucanotransferase into highly insoluble high-amylose starch with a low average degree of polymerization (56-73), in yields as high as 78% over a wide range of temperatures (25-70 degreesC). Ability to undergo this conversion was highly concentrat...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:The aim of this paper is to make the point on the fortieth years study on the β-glycosidase from Sulfolobus solfataricus. This enzyme represents one of the thermophilic biocatalysts, which is more extensively studied as witnessed by the numerous literature reports available since 1980. Comprehensive biochemical studie...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1002/bab.1982
更新日期：2020-07-01 00:00:00
abstract：:Limit of detection (LOD), limit of quantification, and the dynamic range of detection of hepatitis B surface antigen antibody (anti-HBs) using a surface plasmon resonance (SPR) chip-based approach with Pichia pastoris-derived recombinant hepatitis B surface antigen (HBsAg) as recognition element were established throu...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1528
更新日期：2017-09-01 00:00:00
abstract：:We tested a variety of liposomes for parameters such as DNA binding capacity and DNase I protection of incorporated and attached DNA to elucidate their use as vehicles for DNA transfer into cells and animals. The results were compared to other potential DNA vehicles, empty viral capsids, and nanoparticles. Maximal bin...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The application of the hydroperoxidase activity of immobilized lipoxygenase to xenobiotic metabolization is reported in this work. Soya bean lipoxygenase has been immobilized by covalent coupling to oxirane acrylic beads. This immobilized system has been applied to the oxidation of chlorpromazine (CPZ), a phenothiazin...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20020017
更新日期：2002-10-01 00:00:00
abstract：:Sephadex was derivatized consecutively with Staphylococcus Protein A (SpA) and cell-specific antibodies, and the binding of cells to the resulting material was examined. For comparison, cell binding to commercially obtained SpA-Sepharose was determined. Sephadex G-10, carboxylated by reaction with glycine and activate...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20010062
更新日期：2002-02-01 00:00:00
abstract：:Mouse and human erythrocytes are inherently different with respect to slow dialysis encapsulation used in preparing carrier erythrocytes. Fluorescein isothiocyanate (FITC)-dextran was added to five different stages of the encapsulation process to discern when endocytic vesiculation occurred. Mouse erythrocytes were mu...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:A simple and cost-effective method is described to sequence double-stranded DNA (< 100 ng) using a very small amount of fluorescent sequencing mix (one-tenth of manufacturer-recommended amount). The modification has significant advantages over conventional protocols and can be used to sequence, using a single sequenci...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:The arrival of novel health crisis by a novel member of coronavirus group named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by World Health Organization took the whole world in global emergency by affecting 206 countries. The virus infects 206 countries with 86,839,226 confirmed cases, 61,565,949 r...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1002/bab.2101
更新日期：2021-01-12 00:00:00
abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.2006
更新日期：2020-08-15 00:00:00
abstract：:The effects of chitin [(1-->4)-2-acetamido-2-deoxy-beta-D-glucan] and its partially deacetylated derivatives, chitosans, on the human dermal fibroblast-mediated contraction of collagen lattices were examined in vitro as a model for the contraction of cutaneous wounds in vivo. Chitosan CL313A, a short-chain-length 89% ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20020040
更新日期：2002-12-01 00:00:00
abstract：:The main metabolic properties of human red blood cells (RBC) overloaded with glucose catabolizing enzymes such as hexokinase and glucose oxidase were evaluated. Human erythrocytes loaded with human hexokinase metabolized 3.1 +/- 0.2 mumol/h/ml RBC of glucose, an amount double that consumed by normal and unloaded cells...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new ti...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1990-10-01 00:00:00
abstract：:Microparticles have been used for delivery of chemo- and radio-therapeutic agents to tumours in patients for more than a decade, whereas cationic liposomes have been used for introducing nucleic acids into mammalian cells for the same period of time. This minireview discusses the potential of delivering therapeutic ON...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1042/BA20040005
更新日期：2004-10-01 00:00:00
abstract：:The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1986-04-01 00:00:00
abstract：:Arylacetonitrile-hydrolyzing nitrilase (E.C. 3.5.5.5) of Alcaligenes sp. MTCC 10675 has been purified by up to 46-fold to homogeneity and 32% yield using ammonium sulfate fractionation, Sephacryl S-300 gel permeation, and anion exchange chromatography. The molecular weight of the native enzyme was estimated to be 520 ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1192
更新日期：2014-07-01 00:00:00
abstract：:We report a novel surface plasmon resonance (SPR) biosensor that uses the full-length Det7 phage tail protein (Det7T) to rapidly and selectively detect Salmonella enterica serovar Typhimurium (S. Typhimurium). Det7T, which was obtained using recombinant protein expression and purification in Escherichia coli, demonstr...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1883
更新日期：2020-01-08 00:00:00
abstract：:The variation with pH of the kinetic parameters of penicillin acylase from Kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. The pH-dependence of log (V/Km) for penicillin G showed that a group with a pK value over 4.7 must be deprotonated and t...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carra...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1417
更新日期：2016-09-01 00:00:00
abstract：:So far, many studies have focused on biodegradable scaffolds for tissue engineering purposes. Herein, a starch-based biodegradable scaffold was fabricated by the freeze-drying method and cross-linked using a different concentration of 3-glycidoxypropyl-trimethoxysilane (GPTMS). Field emission scanning electron microsc...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1694
更新日期：2019-01-01 00:00:00
abstract：:An amylase-producing psychrotroph bacterium was isolated from soil and identified as belonging to the genus Exiguobacterium. A novel cold-adapted α-amylase, Amy SH3, was purified from culture medium of this bacterium using acetone precipitation and DEAE-Sepharose anion-exchange chromatography. The molecular mass of th...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1140
更新日期：2014-03-01 00:00:00
abstract：:A protein with a molecular mass of 66 kDa was isolated by a simple, rapid, and inexpensive method, using 3-N-morpholinopropanesulfonic acid, potassium thiocyanate, and dithiothreitol, from a mixture of spores, parasporal crystals, and cell debris of Bacillus thuringiensis subsp. kurstaki. The protein was active agains...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:The sialidase gene from Brevibacterium casei was cloned in pET28a and overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3). The histidine-tagged sialidase protein was purified and characterized from the crude cell extracts of isopropyl-β-d-thiogalactopyranoside-induced cells using Ni-NTA agarose c...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1475
更新日期：2017-03-01 00:00:00
abstract：:A new α-neoagarobiose hydrolase (NABH) called AgaWH117 was cloned from Agarivorans gilvus WH0801. The gene encoding this hydrolase consists of 1,086 bp and encodes a protein containing 361 amino acids. This new NABH showed 74% amino acid sequence identity with other known NABHs. The molecular mass of the recombinant A...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1363
更新日期：2016-03-01 00:00:00
abstract：:The participation of Mg2+ or Mn2+ nucleoside diphosphates in the reverse reaction catalyzed by purified carbamate kinase (ATP:carbamate phosphotransferase, EC 2.7.2.2) of Lactobacillus buchneri NCDO110 was studied. The results of initial velocity studies have indicated that Mn2+ ADP is as effective as a substrate as M...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1002/bab.1714
更新日期：2019-03-01 00:00:00
