Production of thymosin alpha1 via non-enzymatic acetylation of the recombinant precursor.

abstract：:Nicotine is a potent parasympathomimetic stimulant and an important natural alkaloid mainly found in the Nicotiana genus of plants. It can directly threaten ecological security and human health in tobacco waste, wastewater, and other forms of tobacco production. Therefore, it is the basis of nicotine pollution prevent...

journal_title：Biotechnology and applied biochemistry

authors： Ruan A,Gao Y,Fang C,Xu Y

更新日期：2018-11-01 00:00:00

abstract：:The objective of this work is to develop structured, segregated stochastic models for bioprocesses using time-series flow cytometric (FC) data. To this end, mammalian CHO cells were grown in both batch and fed-batch cultures, and their viable cell numbers (VCDs), monoclonal antibody (MAb), cell cycle phases, mitochond...

journal_title：Biotechnology and applied biochemistry

authors： Shirsat N,Avesh M,English NJ,Glennon B,Al-Rubeai M

更新日期：2013-09-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:The phosphatase and tensin homolog (PTEN) gene plays a crucial role in signal transduction by negatively regulating the PI3K signaling pathway. It is the most frequent mutated gene in many human-related cancers. Considering its critical role, a functional analysis of missense mutations of PTEN gene was undertaken in t...

journal_title：Biotechnology and applied biochemistry

authors： Khan I,Ansari IA,Singh P,Dass J FP

更新日期：2017-09-01 00:00:00

abstract：:Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin inj...

journal_title：Biotechnology and applied biochemistry

authors： Calcabrini C,Mancini U,De Bellis R,Diaz AR,Martinelli M,Cucchiarini L,Sestili P,Stocchi V,Potenza L

更新日期：2017-05-01 00:00:00

abstract：:The present study elaborates a cost-effective and transfectant-free method for generating recombinant Bombyx mori (silkworm) nucleopolyhedrovirus in silkworm larvae and pupae by injecting invasive Escherichia coli carrying BmBacmid [BmNPV (B. mori nucleopolyhedrovirus)-Bacmid] into larval haemocoel. Up to 109 PFU (pla...

journal_title：Biotechnology and applied biochemistry

authors： Sun J,Yao L,Yao N,Xu H,Jin P,Kan Y

更新日期：2010-12-01 00:00:00

abstract：:High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...

journal_title：Biotechnology and applied biochemistry

authors： Eriksson C,Schwenk JM,Sjöberg A,Hober S

更新日期：2010-06-09 00:00:00

abstract：:Carbamate kinase has been prepared from Lactobacillus buchneri NCDO110. An approximately 91-fold increase in the specific activity of the enzyme was achieved. The purified extract exhibited a single band following polyacrylamide gel electrophoresis. The apparent molecular weight as determined by gel electrophoresis wa...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Nadra Chaud CA,Pesce de Ruiz Holgado A,Oliver G

更新日期：1986-02-01 00:00:00

abstract：:A novel iron superoxide dismutase (Fe-SOD) gene from Jatropha curcas was cloned and expressed in Escherichia coli BL21 (DE3). This recombinant enzyme was isolated by a combined procedure involving immobilized metal-ion affinity chromatography and ion-exchange chromatography. The apparent molecular mass of this purifie...

journal_title：Biotechnology and applied biochemistry

authors： Ou-Yang C,Cai F,Gao S,Niu B,Wang S,Chen F

更新日期：2012-09-01 00:00:00

abstract：:We have explored the adaptation of the cytochromes P450 (P450) of deep-sea bacteria to high hydrostatic pressures. Strict conservation of the protein fold and functional importance of protein-bound water make P450 a unique subject for the studies of high-pressure adaptation. Earlier, we expressed and purified a fatty-...

journal_title：Biotechnology and applied biochemistry

authors： Davydov DR,Sineva EV,Davydova NY,Bartlett DH,Halpert JR

更新日期：2013-01-01 00:00:00

abstract：:Rapid progress has been made in the design and synthesis of oligomers and polymers that emulate the properties of natural proteins. Molecular bioengineering offers the chance to design and produce artificial polymeric proteins with tailored polymeric properties. The elastin-like polypeptides are a well-defined family ...

journal_title：Biotechnology and applied biochemistry

authors： Bandiera A,Taglienti A,Micali F,Pani B,Tamaro M,Crescenzi V,Manzini G

更新日期：2005-12-01 00:00:00

abstract：:Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to...

journal_title：Biotechnology and applied biochemistry

authors： Guzmán-Rodríguez F,Alatorre-Santamaría S,Gómez-Ruiz L,Rodríguez-Serrano G,García-Garibay M,Cruz-Guerrero A

更新日期：2019-03-01 00:00:00

abstract：:Tissue engineering has been conducted in the study of cardiovascular grafts for many years. Many obstacles have been overcome in this rapidly changing field, but one difficulty has remained until now: the large number of endothelial cells (ECs) needed for seeding the inner layer of bypass graft. Recent advances in end...

journal_title：Biotechnology and applied biochemistry

authors： Punshon G,Vara DS,Sales KM,Seifalian AM

更新日期：2011-09-01 00:00:00

abstract：:The interpretation of the circular dichroism (c.d.) spectra of proteins to date requires additional secondary structural information of the proteins to be analysed (e.g., X-ray or n.m.r. data). Therefore these methods are inappropriate for a c.d. database whose secondary structures are unknown, as in the case of the m...

journal_title：Biotechnology and applied biochemistry

authors： Fasman GD

更新日期：1993-10-01 00:00:00

abstract：:The use of glutaraldehyde (GTA) for cross-linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross-linking agent for tissue implants, we have evaluated a number of cross-linking procedures shown previousl...

journal_title：Biotechnology and applied biochemistry

authors： Simmons DM,Kearney JN

更新日期：1993-02-01 00:00:00

abstract：:Cordyceps sinensis (Chinese caterpillar fungus) is a fungus parasitic on the larvae of Lepidoptera and has been considered to be a precious tonic food and herbal medicine since ancient times in China. Recently, some fungal strains have been isolated from the fruiting bodies of wild C. sinensis, and some of them have b...

journal_title：Biotechnology and applied biochemistry

authors： Zhang W,Yang J,Chen J,Hou Y,Han X

更新日期：2005-08-01 00:00:00

abstract：:The dynamic gassing-out method was used to assess the effect of stirrer speed, air flow rate and alginate concentration on the overall volumetric oxygen mass transfer coefficient (K(L)a) in a bench-top stirred fermenter equipped with two Rushton-type turbines. As the alginate concentration in the medium was increased ...

journal_title：Biotechnology and applied biochemistry

authors： Parente E,Piraino P,Fidaleo M,Moresi M

更新日期：2004-10-01 00:00:00

abstract：:Amyloglucosidase from Rhizopus mould and beta-glucosidase from sweet almond were employed for the preparation of phenolic and vitamin glycosides of vanillin, N-vanillylnonanamide, DL-dopa, dopamine, curcumin, alpha-tocopherol (vitamin E), pyridoxine (vitamin B(6)), ergocalciferol (vitamin D(2)), thiamin (vitamin B(1))...

journal_title：Biotechnology and applied biochemistry

authors： Charles RE,Ponrasu T,Sivakumar R,Divakar S

更新日期：2009-03-01 00:00:00

abstract：:HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...

journal_title：Biotechnology and applied biochemistry

authors： Vázquez-Blomquist D,González S,Duarte CA

更新日期：2002-12-01 00:00:00

abstract：:St. John's wort ( Hypericum perforatum L.) is a herbaceous perennial distributed throughout the World that has been widely used in traditional medicine. H. perforatum produces several types of biologically active compound, including the hypericins--a family of light-activated anthraquinones, localized within specializ...

journal_title：Biotechnology and applied biochemistry

authors： Kirakosyan A,Sirvent TM,Gibson DM,Kaufman PB

更新日期：2004-02-01 00:00:00

abstract：:In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carra...

journal_title：Biotechnology and applied biochemistry

authors： Koklukaya SZ,Sezer S,Aksoy S,Hasirci N

更新日期：2016-09-01 00:00:00

abstract：:We have analysed the status and transcriptional activity of the bovine papillomavirus-I (complete BPV-I genome)-based expression vector pCES in CI27i-cell-line-derived 3TI cells used for the industrial production of recombinant human erythropoietin (rhuEpo). Complete tandem head-to-tail integration of about 600 vector...

journal_title：Biotechnology and applied biochemistry

authors： Fibi MR,Kunz J,Weimer T,Schulz R,Teifke P,Grzeschik KH,Johannsen R,Zettlmeissl G

更新日期：1996-02-01 00:00:00

abstract：:The arrival of novel health crisis by a novel member of coronavirus group named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by World Health Organization took the whole world in global emergency by affecting 206 countries. The virus infects 206 countries with 86,839,226 confirmed cases, 61,565,949 r...

journal_title：Biotechnology and applied biochemistry

authors： Shivangi S,Meena LS

更新日期：2021-01-12 00:00:00

abstract：:Higher thermostability or acid resistance for fungal α-amylase will help to improve the sugar-making process and cut down the production costs. Here, the thermostability or acid resistance of Rhizopus oryzae α-amylase (ROAmy) was significantly enhanced by site-directed evolution based on multiple sequence alignment (M...

journal_title：Biotechnology and applied biochemistry

authors： Li S,Yang Q,Tang B

更新日期：2020-07-01 00:00:00

abstract：:Most insulin-dependent diabetes mellitus patients gen-erate conformational autoantibodies to the islet-cell 65-kDa variant of human glutamate decarboxylase (GAD65), and several immunochemical tests for the early detection of type-1 diabetes rely on GAD65 antibody (GADA) assessment using properly folded recombinant GAD...

journal_title：Biotechnology and applied biochemistry

authors： Santos J,Antón EA,Buslje CM,Valdez SN,Villanueva AL,Sica M,Iacono R,Maffia P,Poskus E,Ermácora MR

更新日期：2000-06-01 00:00:00

abstract：:The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as...

journal_title：Biotechnology and applied biochemistry

authors： Cannio R,de Pascale D,Rossi M,Bartolucci S

更新日期：1994-04-01 00:00:00

abstract：:So far, many studies have focused on biodegradable scaffolds for tissue engineering purposes. Herein, a starch-based biodegradable scaffold was fabricated by the freeze-drying method and cross-linked using a different concentration of 3-glycidoxypropyl-trimethoxysilane (GPTMS). Field emission scanning electron microsc...

journal_title：Biotechnology and applied biochemistry

authors： Aidun A,Zamanian A,Ghorbani F

更新日期：2019-01-01 00:00:00

abstract：:The participation of Mg2+ or Mn2+ nucleoside diphosphates in the reverse reaction catalyzed by purified carbamate kinase (ATP:carbamate phosphotransferase, EC 2.7.2.2) of Lactobacillus buchneri NCDO110 was studied. The results of initial velocity studies have indicated that Mn2+ ADP is as effective as a substrate as M...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Pesce de Ruiz Holgado AA,Oliver G

更新日期：1987-04-01 00:00:00

abstract：:A new system equipped with a computer-controlled multiple activity analyzer has been developed for the efficient purification of multiple enzymes. The system consists of the following units: conventional enzyme fractionation system with a peristaltic pump, liquid chromatographic column, fraction collector, and uv moni...

journal_title：Biotechnology and applied biochemistry

authors： Tokushige M,Sasaki S

更新日期：1986-12-01 00:00:00

abstract：:Restoration of insulin secretion by transplantation of isolated islets is a treatment for type Ι diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate e...

journal_title：Biotechnology and applied biochemistry

authors： Li N,Zhang Y,Xiu Z,Wang Y,Chen L,Wang S,Li S,Guo X,Ma X

更新日期：2015-07-01 00:00:00