Abstract:
:Rapid progress has been made in the design and synthesis of oligomers and polymers that emulate the properties of natural proteins. Molecular bioengineering offers the chance to design and produce artificial polymeric proteins with tailored polymeric properties. The elastin-like polypeptides are a well-defined family of polymers with noteworthy characteristic based on the VPGVG repeated motif of bovine elastin. In the human homologue, the most regular sequence is represented by the repetition of the VAPGVG hexapeptidic motif. On the basis of this sequence, a synthetic gene has been designed, cloned and expressed in Escherichia coli to obtain artificial protein polymers. The rapid one-step in-frame cloning of any biologically active sequence can be achieved directly in the expression vector, allowing further improvement of the potential of the resulting product.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Bandiera A,Taglienti A,Micali F,Pani B,Tamaro M,Crescenzi V,Manzini Gdoi
10.1042/BA20050114subject
Has Abstractpub_date
2005-12-01 00:00:00pages
247-56issue
Pt 3eissn
0885-4513issn
1470-8744pii
BA20050114journal_volume
42pub_type
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