Enhanced production of 2,3-butanediol by overexpressing acetolactate synthase and acetoin reductase in Klebsiella pneumoniae.

abstract：:Gene doping can be easily concealed since its product is similar to endogenous protein, making its effective detection very challenging. In this study, we selected insulin-like growth factor I (IGF-I) exogenous gene for gene doping detection. First, the synthetic IGF-I gene was subcloned to recombinant adeno-associate...

journal_title：Biotechnology and applied biochemistry

authors： Zhang JJ,Xu JF,Shen YW,Ma SJ,Zhang TT,Meng QL,Lan WJ,Zhang C,Liu XM

更新日期：2017-07-01 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is a hematologic malignancy characterized by excessive growth of myeloid cells and their progenitors. The proportion of spectrin dimers compared to tetramers extracted from membranes at 4 degrees C, under low ionic strength conditions, increased in CML erythrocytes. These also displa...

journal_title：Biotechnology and applied biochemistry

authors： Basu J,Kundu M,Rakshit MM,Chakrabarti P

更新日期：1990-10-01 00:00:00

abstract：:Marine biomass, especially the algal biomass, is currently considered to be one of the most potential candidates for biofuels conversion during the development of biomass utilization. In this study, a diluted sulfuric acid pretreatment method was established for biobutanol from red algal biomass Gelidium amansii using...

journal_title：Biotechnology and applied biochemistry

authors： Hong Y,Chen C,Wu YR

更新日期：2020-09-01 00:00:00

abstract：:We report a novel surface plasmon resonance (SPR) biosensor that uses the full-length Det7 phage tail protein (Det7T) to rapidly and selectively detect Salmonella enterica serovar Typhimurium (S. Typhimurium). Det7T, which was obtained using recombinant protein expression and purification in Escherichia coli, demonstr...

journal_title：Biotechnology and applied biochemistry

authors： Hyeon SH,Lim WK,Shin HJ

更新日期：2020-01-08 00:00:00

abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...

journal_title：Biotechnology and applied biochemistry

authors： Qiu C,Wang X,Zhang X,Li Z,Zhou Y,Kang J

更新日期：2020-08-15 00:00:00

abstract：:The immobilization of F(0)F(1)-ATPase in uniform orientation is reported. The biotinylated and histidine-tagged subunits of the bacterial F(0)F(1)-ATPase complex were used for immobilization of the complex on artificial semi-permeable membranes resulting in 88+/-7.8 and 72+/-5.2% coupling of the enzymes. The immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2004-06-01 00:00:00

abstract：:A new system equipped with a computer-controlled multiple activity analyzer has been developed for the efficient purification of multiple enzymes. The system consists of the following units: conventional enzyme fractionation system with a peristaltic pump, liquid chromatographic column, fraction collector, and uv moni...

journal_title：Biotechnology and applied biochemistry

authors： Tokushige M,Sasaki S

更新日期：1986-12-01 00:00:00

abstract：:Human thymosin alpha1 is an effective immune system enhancer for the treatment of cancer and viral diseases. Therefore the development of new methods for its synthesis is an urgent problem. In the present work, we propose an efficient scalable scheme for the production of recombinant thymosin alpha1. We used an expres...

journal_title：Biotechnology and applied biochemistry

authors： Esipov RS,Stepanenko VN,Beyrakhova KA,Muravjeva TI,Miroshnikov AI

更新日期：2010-05-19 00:00:00

abstract：:When crude soya-bean oil was used as the source for biodiesel production, the yield of methyl ester was significantly lower than that from refined soya-bean oil. The major difference between refined and crude soya-bean oils was found to be due to the contents of phospholipids, free acid and water, which have varied in...

journal_title：Biotechnology and applied biochemistry

authors： Du W,Xu YY,Zeng J,Liu DH

更新日期：2004-10-01 00:00:00

abstract：:Acid phosphatase (EC 3.1.3.2) from Vigna aconitifolia seeds was purified to apparent homogeneity by using ammonium sulfate fractionation and cation-exchange chromatography [carboxymethyl (CM) cellulose]. The enzyme was 228-fold purified with 14.6% recovery. Analytical gel filtration chromatography on Sephadex G-200 co...

journal_title：Biotechnology and applied biochemistry

authors： Anand A,Srivastava PK

更新日期：2014-03-01 00:00:00

abstract：:This study was designed to investigate the protective effects of Acanthus ilicifolius alkaloid A [4-hydroxy-2-benzoxazolone (HBOA)] and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride (CCl4 )-induced liver fibrosis in rats. Spr...

journal_title：Biotechnology and applied biochemistry

authors： Wai KK,Liang Y,Zhou L,Cai L,Liang C,Liu L,Lin X,Wu H,Lin J

更新日期：2015-07-01 00:00:00

abstract：:Production of His-BbST [hexahistidine-tagged BbST (bubaline somatotropin)] by auto-induction in high-density shake-flask cultures coupled with a single-step, on-column purification and refolding strategy is described here. To optimize expression of BbST, different media and expression conditions were tested. The highe...

journal_title：Biotechnology and applied biochemistry

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2007-05-01 00:00:00

abstract：:Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our p...

journal_title：Biotechnology and applied biochemistry

authors： Chen Z,Zhuo W,Wang Y,Ao X,An J

更新日期：2008-06-01 00:00:00

abstract：:Monoclonal antibodies specific to human prolactin were generated by an improved hybridoma technique. Following immunization with hormone conjugated with hemocyanin and cell fusion, hybrid cells were first cultured in a semisolid medium containing methylcellulose and later transferred to liquid medium for subculture. O...

journal_title：Biotechnology and applied biochemistry

authors： Lee CY,Chow SN,Coleman P,DeTuri MP

更新日期：1987-02-01 00:00:00

abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...

journal_title：Biotechnology and applied biochemistry

authors： Wen CM,Tseng CS,Cheng CY,Li YK

更新日期：2002-06-01 00:00:00

abstract：:We have explored the adaptation of the cytochromes P450 (P450) of deep-sea bacteria to high hydrostatic pressures. Strict conservation of the protein fold and functional importance of protein-bound water make P450 a unique subject for the studies of high-pressure adaptation. Earlier, we expressed and purified a fatty-...

journal_title：Biotechnology and applied biochemistry

authors： Davydov DR,Sineva EV,Davydova NY,Bartlett DH,Halpert JR

更新日期：2013-01-01 00:00:00

abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...

journal_title：Biotechnology and applied biochemistry

authors： Hiragami F,Akiyama J,Koike Y,Kano Y

更新日期：2007-05-01 00:00:00

abstract：:High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...

journal_title：Biotechnology and applied biochemistry

authors： Eriksson C,Schwenk JM,Sjöberg A,Hober S

更新日期：2010-06-09 00:00:00

abstract：:Higher thermostability or acid resistance for fungal α-amylase will help to improve the sugar-making process and cut down the production costs. Here, the thermostability or acid resistance of Rhizopus oryzae α-amylase (ROAmy) was significantly enhanced by site-directed evolution based on multiple sequence alignment (M...

journal_title：Biotechnology and applied biochemistry

authors： Li S,Yang Q,Tang B

更新日期：2020-07-01 00:00:00

abstract：:Among the heat-shock proteins (HSPs) induced in response to heat-shock (HS) in all organisms, the low-molecular-mass (LMM) HSPs are the most abundant and unique in plants. The HSP genes at the transcriptional and the translational levels have been well-characterized; however, relatively little is known about the physi...

journal_title：Biotechnology and applied biochemistry

authors： Yeh KW,Jinn TL,Yeh CH,Chen YM,Lin CY

更新日期：1994-02-01 00:00:00

abstract：:Melamine adulteration of food is a public health concern. It has been seen that melamine causes disease in many organs. Melamine-induced kidney disease is a well-recognized clinicopathological entity. Inflammation is thought to be important in melamine-induced pathology. Melamine is expected to bind with albumin becau...

journal_title：Biotechnology and applied biochemistry

authors： Rajpoot M,Bhattacharyya R,Banerjee D,Sharma A

更新日期：2017-07-01 00:00:00

abstract：:An extremely thermostable UDP-GlcNAc pyrophosphorylase has been purified from Thermus caldophilus GK24 by chromatographic methods including ion-exchange, hydrophobic interaction, and affinity chromatographies. The specific activity of the enzyme was enriched 41.8-fold, with a recovery of 2%. The molecular mass of the ...

journal_title：Biotechnology and applied biochemistry

authors： Kim JS,Koh S,Shin HJ,Lee DS,Lee SY

更新日期：1999-02-01 00:00:00

abstract：:Bacillus subtilis strain DM-03, which is isolated from starter culture used for the production of alcohol by local Assam tribes, grows optimally at 52-55 degrees C and secretes a significant amount of alpha-amylase at pH 8.0 into the culture media. This alpha-amylase, purified by ion-exchange, gel-filtration and rever...

journal_title：Biotechnology and applied biochemistry

authors： Das K,Doley R,Mukherjee AK

更新日期：2004-12-01 00:00:00

abstract：:The aim of this paper is to make the point on the fortieth years study on the β-glycosidase from Sulfolobus solfataricus. This enzyme represents one of the thermophilic biocatalysts, which is more extensively studied as witnessed by the numerous literature reports available since 1980. Comprehensive biochemical studie...

journal_title：Biotechnology and applied biochemistry

authors： Febbraio F,Ionata E,Marcolongo L

更新日期：2020-07-01 00:00:00

abstract：:HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...

journal_title：Biotechnology and applied biochemistry

authors： Vázquez-Blomquist D,González S,Duarte CA

更新日期：2002-12-01 00:00:00

abstract：:To reduce the degradation pathway of rhIFN-gamma (recombinant human interferon-gamma), the susceptibility against oxidative stress during fermentation in Escherichia coli cells and in purification process were investigated. Fermentations of recombinant E. coli were performed at 5, 30 and 60% DO (dissolved oxygen) conc...

journal_title：Biotechnology and applied biochemistry

authors： Mohammadian-Mosaabadi J,Naderi-Manesh H,Maghsoudi N,Khalilzadeh R,Shojaosadati SA,Ebrahimi M

更新日期：2005-02-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:alpha-Cyclodextrin (CD) was converted by Bacillus macerans cyclodextrin glucanotransferase into highly insoluble high-amylose starch with a low average degree of polymerization (56-73), in yields as high as 78% over a wide range of temperatures (25-70 degreesC). Ability to undergo this conversion was highly concentrat...

journal_title：Biotechnology and applied biochemistry

authors： Rendleman JA Jr,Knutson CA Jr

更新日期：1998-12-01 00:00:00

abstract：:A pathway for the synthesis of the repeating tetrasaccharide units in gellan gum from Pseudomonas elodea is proposed. The enzymes presumed to be involved in the synthesis of the activated precursors UDP-glucose, TDP-rhamnose, and UDP-glucuronic acid were detected and assayed in crude cell extracts of the gellan-produc...

journal_title：Biotechnology and applied biochemistry

authors： Martins LO,Sá-Correia I

更新日期：1991-12-01 00:00:00

abstract：:Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (E...

journal_title：Biotechnology and applied biochemistry

authors： Lau MF,Chua KH,Sabaratnam V,Kuppusamy UR

更新日期：2020-08-28 00:00:00