Expression and purification of a secreted functional mouse/human chimaeric antibody against bacterial endotoxin in baculovirus-infected insect cells.

abstract：:Human erythrocytes from ten patients with chronic obstructive pulmonary disease (COPD) were loaded with increasing amounts of dexamethasone 21-phosphate and were re-infused into the original donors. Drug-loaded erythrocytes acted as circulating bioreactors, converting the non-diffusible dexamethasone 21-phosphate into...

journal_title：Biotechnology and applied biochemistry

authors： Rossi L,Serafini S,Cenerini L,Picardi F,Bigi L,Panzani I,Magnani M

更新日期：2001-04-01 00:00:00

abstract：:Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for bo...

journal_title：Biotechnology and applied biochemistry

authors： Parascandola P,de Alteriis E

更新日期：1996-02-01 00:00:00

abstract：:Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new ti...

journal_title：Biotechnology and applied biochemistry

authors： Tien HT,Salamon Z

更新日期：1990-10-01 00:00:00

abstract：:Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, the molecular cause of the disease is poorly elucidated. In the present study, we primarily found that translationally controlled tumor protein (TCTP) was overexpressed in human OS tissues and cell lines. To investigate t...

journal_title：Biotechnology and applied biochemistry

authors： Shen JH,Qu CB,Chu HK,Cui MY,Wang YL,Sun YX,Song YD,Li G,Shi FJ

更新日期：2016-01-01 00:00:00

abstract：:Gene doping can be easily concealed since its product is similar to endogenous protein, making its effective detection very challenging. In this study, we selected insulin-like growth factor I (IGF-I) exogenous gene for gene doping detection. First, the synthetic IGF-I gene was subcloned to recombinant adeno-associate...

journal_title：Biotechnology and applied biochemistry

authors： Zhang JJ,Xu JF,Shen YW,Ma SJ,Zhang TT,Meng QL,Lan WJ,Zhang C,Liu XM

更新日期：2017-07-01 00:00:00

abstract：:Most insulin-dependent diabetes mellitus patients gen-erate conformational autoantibodies to the islet-cell 65-kDa variant of human glutamate decarboxylase (GAD65), and several immunochemical tests for the early detection of type-1 diabetes rely on GAD65 antibody (GADA) assessment using properly folded recombinant GAD...

journal_title：Biotechnology and applied biochemistry

authors： Santos J,Antón EA,Buslje CM,Valdez SN,Villanueva AL,Sica M,Iacono R,Maffia P,Poskus E,Ermácora MR

更新日期：2000-06-01 00:00:00

abstract：:Entrapped cells and entrapped cells grown inside of a calcium alginate matrix as well as free cells have been investigated using Saccharomyces cerevisiae mutant strains with regard to their pattern of growth and invertase activity. The repression of invertase by glucose and glucose-consumption ability were considered ...

journal_title：Biotechnology and applied biochemistry

authors： Rossi-Alva JC,Rocha-Leão MH

更新日期：2003-08-01 00:00:00

abstract：:An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferul...

journal_title：Biotechnology and applied biochemistry

authors： Kroon PA,Faulds CB,Williamson G

更新日期：1996-06-01 00:00:00

abstract：:Higher thermostability or acid resistance for fungal α-amylase will help to improve the sugar-making process and cut down the production costs. Here, the thermostability or acid resistance of Rhizopus oryzae α-amylase (ROAmy) was significantly enhanced by site-directed evolution based on multiple sequence alignment (M...

journal_title：Biotechnology and applied biochemistry

authors： Li S,Yang Q,Tang B

更新日期：2020-07-01 00:00:00

abstract：:The main metabolic properties of human red blood cells (RBC) overloaded with glucose catabolizing enzymes such as hexokinase and glucose oxidase were evaluated. Human erythrocytes loaded with human hexokinase metabolized 3.1 +/- 0.2 mumol/h/ml RBC of glucose, an amount double that consumed by normal and unloaded cells...

journal_title：Biotechnology and applied biochemistry

authors： Rossi L,Bianchi M,Magnani M

更新日期：1992-04-01 00:00:00

abstract：:Naproxen and bezafibrate fall into the category of pharmaceuticals that have been widely detected in the aquatic environment, and one of the major sources is the effluent discharge from wastewater treatment plants. This study investigated the sorption and degradation kinetics of naproxen and bezafibrate in the presenc...

journal_title：Biotechnology and applied biochemistry

authors： Tang Y,Li XM,Xu ZC,Guo QW,Hong CY,Bing YX

更新日期：2014-05-01 00:00:00

abstract：:A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN-DI) that happened during the long-term storage process. It was found that FMN-DI did not simpl...

journal_title：Biotechnology and applied biochemistry

authors： Huston S,Collins J,Sun F,Zhang T,Vaden TD,Zhang YP,Fu J

更新日期：2018-05-01 00:00:00

abstract：:Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and ...

journal_title：Biotechnology and applied biochemistry

authors： Karuppaiya P,Tsay HS

更新日期：2020-01-01 00:00:00

abstract：:The human placenta is a rich raw material for production of many biopharmaceutical products. Here we describe a co-purification process for the production of four different proteins from haemolysed human placenta blood: IgG, catalase (Cat), superoxide dismutase (Sod) and albumin (Alb). The process can be divided in tw...

journal_title：Biotechnology and applied biochemistry

authors： Grellet S,Martins EA,Maimoni Gonçalves V,Yague Lopes AP,Raw I,Cabrera-Crespo J

更新日期：2001-12-01 00:00:00

abstract：:Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (E...

journal_title：Biotechnology and applied biochemistry

authors： Lau MF,Chua KH,Sabaratnam V,Kuppusamy UR

更新日期：2020-08-28 00:00:00

abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...

journal_title：Biotechnology and applied biochemistry

authors： Ramström M,Zuberovic A,Grönwall C,Hanrieder J,Bergquist J,Hober S

更新日期：2009-02-01 00:00:00

abstract：:To reduce the degradation pathway of rhIFN-gamma (recombinant human interferon-gamma), the susceptibility against oxidative stress during fermentation in Escherichia coli cells and in purification process were investigated. Fermentations of recombinant E. coli were performed at 5, 30 and 60% DO (dissolved oxygen) conc...

journal_title：Biotechnology and applied biochemistry

authors： Mohammadian-Mosaabadi J,Naderi-Manesh H,Maghsoudi N,Khalilzadeh R,Shojaosadati SA,Ebrahimi M

更新日期：2005-02-01 00:00:00

abstract：:A novel iron superoxide dismutase (Fe-SOD) gene from Jatropha curcas was cloned and expressed in Escherichia coli BL21 (DE3). This recombinant enzyme was isolated by a combined procedure involving immobilized metal-ion affinity chromatography and ion-exchange chromatography. The apparent molecular mass of this purifie...

journal_title：Biotechnology and applied biochemistry

authors： Ou-Yang C,Cai F,Gao S,Niu B,Wang S,Chen F

更新日期：2012-09-01 00:00:00

abstract：:We studied, for the first time, characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of enzyme conformational stability and structural behaviour induced by temperature as a function of pH using enzymic assays, differential scanning calorimetry, fluo...

journal_title：Biotechnology and applied biochemistry

authors： Acosta N,Beldarraín A,Rodríguez L,Alonso Y

更新日期：2000-12-01 00:00:00

abstract：:The survival of inoculated microbes is critical for successful bioaugmentation in wastewater treatment. The influence of readily available nutrients (RANs) on the colonization of two functional bacteria, Pseudomonas putida M9, a strong biofilm-forming strain, and Comamonas testosteroni A3, a 3,5-dinitrobenzoic acid (3...

journal_title：Biotechnology and applied biochemistry

authors： Cheng Z,Meng X,Xie L,Xu H,Li M

更新日期：2015-09-01 00:00:00

abstract：:A new microbial transglutaminase (EC 2.3.2.13) from a Bacillus circulans strain isolated from the aquatic Amazonian environment was purified and characterized. Enzyme purification started with (NH(4))(2)SO(4) 'salting out' and proceeded with liquid chromatography on Q-Sepharose FF and octyl-Sepharose 4 FF. The purific...

journal_title：Biotechnology and applied biochemistry

authors： de Barros Soares LH,Assmann F,Záchia Ayub MA

更新日期：2003-06-01 00:00:00

abstract：:Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in de...

journal_title：Biotechnology and applied biochemistry

authors： Chen L,Guo S,Wu L,Hao C,Xu W,Zhang J

更新日期：2015-05-01 00:00:00

abstract：:As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...

journal_title：Biotechnology and applied biochemistry

authors： Hasegawa M,Kaku T,Kuroda M,Ise N,Kitano H

更新日期：1992-02-01 00:00:00

abstract：:The urinary C-terminal telopeptide fragment of type II collagen (uCTX-II) has been reported as the efficient blood-based biomarker for osteoarthritis, which affects knees, hands, spine, and hips. This study reports a sensing strategy with antibody-conjugated gold nanoparticles (GNP) on an interdigitated electrode (IDE...

journal_title：Biotechnology and applied biochemistry

authors： Wang S,Su S,Yu C,Gopinath SCB,Yang Z

更新日期：2020-07-04 00:00:00

abstract：:A sensitive sandwich enzyme immunoassay (e.i.a.) for serrapeptase (TSP), an orally available anti-inflammatory proteinase, was established using affinity-purified anti-TSP rabbit IgG and its Fab' fragment conjugated with horseradish peroxidase as the first and the second antibodies respectively. TSP in the plasma was ...

journal_title：Biotechnology and applied biochemistry

authors： Moriya N,Nakata M,Nakamura M,Takaoka M,Iwasa S,Kato K,Kakinuma A

更新日期：1994-08-01 00:00:00

abstract：:This review article focuses on the use of the methylotrophic yeast Pichia pastoris as a recombinant protein-expression system. P. pastoris is a useful system for the expression of milligram-to-gram quantities of a protein, which can be scaled up to fermentation to meet greater demands. Compared with mammalian cells, P...

journal_title：Biotechnology and applied biochemistry

authors： Fischer R,Drossard J,Emans N,Commandeur U,Hellwig S

更新日期：1999-10-01 00:00:00

abstract：:Future developments in gene therapy and DNA vaccination depend on cost-effective large-scale production of pharmaceutical-grade pDNA (plasmid DNA). Given the large amount of impurities present in the feedstock, purification processes that have high specificity and capacity at a moderate cost are required. In the prese...

journal_title：Biotechnology and applied biochemistry

authors： Frerix A,Müller M,Kula MR,Hubbuch J

更新日期：2005-08-01 00:00:00

abstract：:In the present study, recombinant HCV (hepatitis C virus) core proteins enhanced the immune response elicited by a co-delivered DNA vaccine encoding HCV core and envelope proteins. A mixture of the plasmid pIDKE2 and Co.173, a protein comprising the first 173 amino acids of HCV core, in particular induces a strong hum...

journal_title：Biotechnology and applied biochemistry

authors： Alvarez-Lajonchere L,González M,Alvarez-Obregón JC,Guerra I,Viña A,Acosta-Rivero N,Musacchio A,Morales J,Dueñas-Carrera S

更新日期：2006-04-01 00:00:00

abstract：:Diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase has been isolated previously using classical protein isolation techniques [A. Guranowski and S. Blanquet (1985) J. Biol. Chem. 260, 3542-3547]. A protein A-Sepharose immunoaffinity column was prepared to simplify the purification procedure. The immunoaffini...

journal_title：Biotechnology and applied biochemistry

authors： Avila DM,Kaushal V,Barnes LD

更新日期：1990-06-01 00:00:00

abstract：:The arrival of novel health crisis by a novel member of coronavirus group named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by World Health Organization took the whole world in global emergency by affecting 206 countries. The virus infects 206 countries with 86,839,226 confirmed cases, 61,565,949 r...

journal_title：Biotechnology and applied biochemistry

authors： Shivangi S,Meena LS

更新日期：2021-01-12 00:00:00