Cloning, overexpression, purification, and characterization of a new iron superoxide dismutase from Jatropha curcas.

abstract：:A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN-DI) that happened during the long-term storage process. It was found that FMN-DI did not simpl...

journal_title：Biotechnology and applied biochemistry

authors： Huston S,Collins J,Sun F,Zhang T,Vaden TD,Zhang YP,Fu J

更新日期：2018-05-01 00:00:00

abstract：:The development of subunit vaccines is presently the main strategy being evaluated for prevention of infectious diseases. The use of recombinant-DNA techniques has facilitated the development of new principles for design and production of subunit vaccines. First of all, the properties of a target protein immunogen can...

journal_title：Biotechnology and applied biochemistry

authors： Hansson M,Nygren PA,Ståhl S

更新日期：2000-10-01 00:00:00

abstract：:Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by bacterial fermentation and chemical and enzymatic processing is currently under development. This stu...

journal_title：Biotechnology and applied biochemistry

authors： Suwan J,Torelli A,Onishi A,Dordick JS,Linhardt RJ

更新日期：2012-11-01 00:00:00

abstract：:The introduction of different methodologies for the construction and screening of complex protein libraries has provided powerful means in protein engineering for the development of molecules with desired traits. A challenge faced in many situations is to adapt a given methodology for efficient and rapid identificatio...

journal_title：Biotechnology and applied biochemistry

authors： Löfdahl PA,Nygren PA

更新日期：2010-03-05 00:00:00

abstract：:Carbamate kinase has been prepared from Lactobacillus buchneri NCDO110. An approximately 91-fold increase in the specific activity of the enzyme was achieved. The purified extract exhibited a single band following polyacrylamide gel electrophoresis. The apparent molecular weight as determined by gel electrophoresis wa...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Nadra Chaud CA,Pesce de Ruiz Holgado A,Oliver G

更新日期：1986-02-01 00:00:00

abstract：:Penicillium ochrochloron Biourge, which was isolated from rotten sweet potato, can produce plant cell wall-degrading enzymes (PCWDEs) with high viscosity reducing capability for ethanol production using fresh sweet potato tubers as feedstock. The enzyme preparation was characterized by a broad enzyme spectrum includin...

journal_title：Biotechnology and applied biochemistry

authors： Huang Y,Jin Y,Shen W,Fang Y,Zhang G,Zhao H

更新日期：2014-07-01 00:00:00

abstract：:The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With ...

journal_title：Biotechnology and applied biochemistry

authors： Hou KC,Zaniewski R

更新日期：1990-06-01 00:00:00

abstract：:Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in de...

journal_title：Biotechnology and applied biochemistry

authors： Chen L,Guo S,Wu L,Hao C,Xu W,Zhang J

更新日期：2015-05-01 00:00:00

abstract：:The objective of this work is to develop structured, segregated stochastic models for bioprocesses using time-series flow cytometric (FC) data. To this end, mammalian CHO cells were grown in both batch and fed-batch cultures, and their viable cell numbers (VCDs), monoclonal antibody (MAb), cell cycle phases, mitochond...

journal_title：Biotechnology and applied biochemistry

authors： Shirsat N,Avesh M,English NJ,Glennon B,Al-Rubeai M

更新日期：2013-09-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:The survival of inoculated microbes is critical for successful bioaugmentation in wastewater treatment. The influence of readily available nutrients (RANs) on the colonization of two functional bacteria, Pseudomonas putida M9, a strong biofilm-forming strain, and Comamonas testosteroni A3, a 3,5-dinitrobenzoic acid (3...

journal_title：Biotechnology and applied biochemistry

authors： Cheng Z,Meng X,Xie L,Xu H,Li M

更新日期：2015-09-01 00:00:00

abstract：:The aim of this paper is to make the point on the fortieth years study on the β-glycosidase from Sulfolobus solfataricus. This enzyme represents one of the thermophilic biocatalysts, which is more extensively studied as witnessed by the numerous literature reports available since 1980. Comprehensive biochemical studie...

journal_title：Biotechnology and applied biochemistry

authors： Febbraio F,Ionata E,Marcolongo L

更新日期：2020-07-01 00:00:00

abstract：:The interaction of four lectins from crops of the legume family with Saccharomyces cerevisiae alpha-mannan, and also with two glycoenzymes containing mainly alpha-mannan moieties, has been studied. The interaction was characterized by a quantitative precipitation assay. The results of precipitation differ with respect...

journal_title：Biotechnology and applied biochemistry

authors： Mislovicová D,Gemeiner P,Sandula J,Masárová J,Vikartovská A,Docolomanský P

更新日期：2000-04-01 00:00:00

abstract：:This paper is concerned with the optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Auricularia polytricha by one-factor-at-a-time and uniform design (UD) methods. First, the one-factor-at-a-time method was adopted to investigate the effects of environmental factors (i.e....

journal_title：Biotechnology and applied biochemistry

authors： Xu CP,Yun JW

更新日期：2003-10-01 00:00:00

abstract：:The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a plant species known for its production of TIAs (terpenoid indole alkaloids), many of which are pharmaceutically important. Ajmalicine and serpentine are prescribed for the treatment of hypertension, whereas the bisindoles vinblastine, vincristine and 3',...

journal_title：Biotechnology and applied biochemistry

authors： Zhou ML,Shao JR,Tang YX

更新日期：2009-04-01 00:00:00

abstract：:Complex-formation between a truncated staphylococcal Protein A produced in Escherichia coli and a native E coli molecular chaperone, DnaK, can be used for the purification of DnaK by IgG-affinity chromatography. The half-time constant for in vitro formation of the Protein A-DnaK complex is about 14 min. Complex-format...

journal_title：Biotechnology and applied biochemistry

authors： Gustavsson K,Bergman T,Veide A,Enfors SO

更新日期：1997-04-01 00:00:00

abstract：:The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...

journal_title：Biotechnology and applied biochemistry

authors： Son HJ,Heo MS,Kim YG,Lee SJ

更新日期：2001-02-01 00:00:00

abstract：:A novel, improved dual bacterial-expression system, designed for large-scale generation of high-quality polyclonal antibody preparations intended for proteomics research, is presented. The concept involves parallel expression of cDNA-encoded proteins, as a fusion with two different tags in two separate vector systems....

journal_title：Biotechnology and applied biochemistry

authors： Falk R,Agaton C,Kiesler E,Jin S,Wieslander L,Visa N,Hober S,Ståhl S

更新日期：2003-12-01 00:00:00

abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...

journal_title：Biotechnology and applied biochemistry

authors： Wen CM,Tseng CS,Cheng CY,Li YK

更新日期：2002-06-01 00:00:00

abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...

journal_title：Biotechnology and applied biochemistry

authors： Hiragami F,Akiyama J,Koike Y,Kano Y

更新日期：2007-05-01 00:00:00

abstract：:c-Jun is a highly oncogenic transcription factor involved in the development of different types of cancer. In the present study we have generated c-Jun-binding-affinity proteins from a phage-displayed library of so-called 'Affibody ligands', developed by combinatorial engineering of a non-immunoglobulin-based scaffold...

journal_title：Biotechnology and applied biochemistry

authors： Lundberg E,Brismar H,Gräslund T

更新日期：2009-01-01 00:00:00

abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...

journal_title：Biotechnology and applied biochemistry

authors： Banks JG,Board RG,Sparks NH

更新日期：1986-04-01 00:00:00

abstract：:Human erythrocytes from ten patients with chronic obstructive pulmonary disease (COPD) were loaded with increasing amounts of dexamethasone 21-phosphate and were re-infused into the original donors. Drug-loaded erythrocytes acted as circulating bioreactors, converting the non-diffusible dexamethasone 21-phosphate into...

journal_title：Biotechnology and applied biochemistry

authors： Rossi L,Serafini S,Cenerini L,Picardi F,Bigi L,Panzani I,Magnani M

更新日期：2001-04-01 00:00:00

abstract：:Cardiolipin, the primary lipid hapten in the antigen suspension used for the detection of antitreponemal antibodies in the sera of syphilitic patients, was successfully coupled to glucose oxidase, peroxidase, and some other enzymes using different crosslinking agents. These complexes were used to replace the pure unco...

journal_title：Biotechnology and applied biochemistry

authors： Ngeh-Ngwainbi J,Kuan SS,Steinman F,Guilbault GG

更新日期：1986-12-01 00:00:00

abstract：:Arylacetonitrile-hydrolyzing nitrilase (E.C. 3.5.5.5) of Alcaligenes sp. MTCC 10675 has been purified by up to 46-fold to homogeneity and 32% yield using ammonium sulfate fractionation, Sephacryl S-300 gel permeation, and anion exchange chromatography. The molecular weight of the native enzyme was estimated to be 520 ...

journal_title：Biotechnology and applied biochemistry

authors： Bhatia SK,Mehta PK,Bhatia RK,Bhalla TC

更新日期：2014-07-01 00:00:00

abstract：:A sensitive sandwich enzyme immunoassay (e.i.a.) for serrapeptase (TSP), an orally available anti-inflammatory proteinase, was established using affinity-purified anti-TSP rabbit IgG and its Fab' fragment conjugated with horseradish peroxidase as the first and the second antibodies respectively. TSP in the plasma was ...

journal_title：Biotechnology and applied biochemistry

authors： Moriya N,Nakata M,Nakamura M,Takaoka M,Iwasa S,Kato K,Kakinuma A

更新日期：1994-08-01 00:00:00

abstract：:It is now a well-known fact that alpha-CD (alpha-cyclodextrin) and Ca(2+) are two important folding agents in the re-activation and refolding processes of many denatured enzyme solutions. Bacillus sp. alpha-amylase was extensively denatured in a 6 M guanidinium chloride solution overnight. Under suitable renaturation ...

journal_title：Biotechnology and applied biochemistry

authors： Yazdanparast R,Khodarahmi R

更新日期：2005-04-01 00:00:00

abstract：:The pH optimum, temperature-dependence, thermal stability, substrate specificity and subsite affinities of the 66 kDa, pI 4.0 glucoamylase of the filamentous fungus Trichoderma reesei were determined. It had a pH optimum of 5.5 and a temperature optimum (5 min reaction time) of 70 degrees C with soluble starch as subs...

journal_title：Biotechnology and applied biochemistry

authors： Fagerström R,Kalkkinen N

更新日期：1995-04-01 00:00:00

abstract：:Cordyceps sinensis (Chinese caterpillar fungus) is a fungus parasitic on the larvae of Lepidoptera and has been considered to be a precious tonic food and herbal medicine since ancient times in China. Recently, some fungal strains have been isolated from the fruiting bodies of wild C. sinensis, and some of them have b...

journal_title：Biotechnology and applied biochemistry

authors： Zhang W,Yang J,Chen J,Hou Y,Han X

更新日期：2005-08-01 00:00:00

abstract：:Mechano-growth factor (MGF), an alternative splicing variant of insulin-like growth factor-1 (IGF-1) gene, promotes cell proliferation and inhibits cell differentiation. It also plays an important role in tumor development. It is important to optimize the production process and achieve MGF protein because there is no ...

journal_title：Biotechnology and applied biochemistry

authors： Feng J,Wan R,Yi Q,He L,Yang L,Tang L

更新日期：2015-09-01 00:00:00