Purification and properties of a transglutaminase produced by a Bacillus circulans strain isolated from the Amazon environment.

abstract：:Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new ti...

journal_title：Biotechnology and applied biochemistry

authors： Tien HT,Salamon Z

更新日期：1990-10-01 00:00:00

abstract：:Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to...

journal_title：Biotechnology and applied biochemistry

authors： Guzmán-Rodríguez F,Alatorre-Santamaría S,Gómez-Ruiz L,Rodríguez-Serrano G,García-Garibay M,Cruz-Guerrero A

更新日期：2019-03-01 00:00:00

abstract：:Human urokinase was immobilized on an ethylene vinyl acetate copolymer surface. Soluble urokinase showed its maximum activity at pH 8.5, while the immobilized enzyme was most active at pH 9.0. Apparently, the shift in optimal pH was due to the polyanionic nature of the carrier surface on which the enzyme was immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Yabushita Y

更新日期：1988-06-01 00:00:00

abstract：:Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (E...

journal_title：Biotechnology and applied biochemistry

authors： Lau MF,Chua KH,Sabaratnam V,Kuppusamy UR

更新日期：2020-08-28 00:00:00

abstract：:Acid phosphatase (EC 3.1.3.2) from Vigna aconitifolia seeds was purified to apparent homogeneity by using ammonium sulfate fractionation and cation-exchange chromatography [carboxymethyl (CM) cellulose]. The enzyme was 228-fold purified with 14.6% recovery. Analytical gel filtration chromatography on Sephadex G-200 co...

journal_title：Biotechnology and applied biochemistry

authors： Anand A,Srivastava PK

更新日期：2014-03-01 00:00:00

abstract：:Biologically active membrane proteins are difficult to isolate. Very often the isolated membrane proteins have low binding affinity or no biological integrity at all. Despite some success in isolation, one has to overcome the hurdles of obtaining sufficient quantity of the proteins and maintaining biological activity ...

journal_title：Biotechnology and applied biochemistry

authors： Wong RB,Lee AH,Rangan VS,Cheng KC

更新日期：2009-05-01 00:00:00

abstract：:In this study, various constructs and hosts were used to produce high levels of cecropin B2 (cecB2). To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin-binding domain and an intein self-cleavage motif in front of cecropin B2, without...

journal_title：Biotechnology and applied biochemistry

authors： Fang YT,Lai WS,Liu JH,Liu YC

更新日期：2019-03-01 00:00:00

abstract：:Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and ...

journal_title：Biotechnology and applied biochemistry

authors： Karuppaiya P,Tsay HS

更新日期：2020-01-01 00:00:00

abstract：:The present study elaborates a cost-effective and transfectant-free method for generating recombinant Bombyx mori (silkworm) nucleopolyhedrovirus in silkworm larvae and pupae by injecting invasive Escherichia coli carrying BmBacmid [BmNPV (B. mori nucleopolyhedrovirus)-Bacmid] into larval haemocoel. Up to 109 PFU (pla...

journal_title：Biotechnology and applied biochemistry

authors： Sun J,Yao L,Yao N,Xu H,Jin P,Kan Y

更新日期：2010-12-01 00:00:00

abstract：:The hydrolysis kinetics of native and denatured haemoglobin, using pepsin immobilized on aluminium oxide, was studied in order to produce hydrolysates containing bioactive peptides. Pepsin was immobilized on acidic alumina and on 2-ethanolamine- O -phosphate (2-EAOP)-modified acidic alumina. Surface charge of the two ...

journal_title：Biotechnology and applied biochemistry

authors： Ticu EL,Vercaigne-Marko D,Huma A,Artenie V,Toma O,Guillochon D

更新日期：2004-04-01 00:00:00

abstract：:The structural underpinnings of O₂ activation in P450s have been limited by the inability to trap unstable oxy complexes in the crystalline state for structure determination. To date there are only two known oxy P450 structures. Even so, much is known about O₂ activation in P450cam and the role that redox partner bind...

journal_title：Biotechnology and applied biochemistry

authors： Poulos TL,Madrona Y

更新日期：2013-01-01 00:00:00

abstract：:We studied, for the first time, characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of enzyme conformational stability and structural behaviour induced by temperature as a function of pH using enzymic assays, differential scanning calorimetry, fluo...

journal_title：Biotechnology and applied biochemistry

authors： Acosta N,Beldarraín A,Rodríguez L,Alonso Y

更新日期：2000-12-01 00:00:00

abstract：:The participation of Mg2+ or Mn2+ nucleoside diphosphates in the reverse reaction catalyzed by purified carbamate kinase (ATP:carbamate phosphotransferase, EC 2.7.2.2) of Lactobacillus buchneri NCDO110 was studied. The results of initial velocity studies have indicated that Mn2+ ADP is as effective as a substrate as M...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Pesce de Ruiz Holgado AA,Oliver G

更新日期：1987-04-01 00:00:00

abstract：:The ability of Acidithiobacillus ferrooxidans to oxidize ferrous iron has been extensively studied in bioleaching to recover metal resources. Although immobilization of A. ferrooxidans is of great importance to achieve high bioleaching performance in practical application, the reported approaches of immobilization of ...

journal_title：Biotechnology and applied biochemistry

authors： Zhu N,Shi C,Shang R,Yang C,Xu Z,Wu P

更新日期：2017-09-01 00:00:00

abstract：:The arrival of novel health crisis by a novel member of coronavirus group named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by World Health Organization took the whole world in global emergency by affecting 206 countries. The virus infects 206 countries with 86,839,226 confirmed cases, 61,565,949 r...

journal_title：Biotechnology and applied biochemistry

authors： Shivangi S,Meena LS

更新日期：2021-01-12 00:00:00

abstract：:This review article focuses on the use of the methylotrophic yeast Pichia pastoris as a recombinant protein-expression system. P. pastoris is a useful system for the expression of milligram-to-gram quantities of a protein, which can be scaled up to fermentation to meet greater demands. Compared with mammalian cells, P...

journal_title：Biotechnology and applied biochemistry

authors： Fischer R,Drossard J,Emans N,Commandeur U,Hellwig S

更新日期：1999-10-01 00:00:00

abstract：:In the erythrocyte membrane, the mobility of band 3 protein, the receptor for concanavalin A (Con A), is drastically reduced by the membrane skeleton. Yet, the vesicles free of membrane skeletal proteins, isolated from the highly agglutinable proteinase-treated cells, are found to be devoid of Con A agglutinability. T...

journal_title：Biotechnology and applied biochemistry

authors： Pestonjamasp KN,Gokhale SM,Mehta NG

更新日期：1990-10-01 00:00:00

abstract：:Mustard tuber wastewater is characterized by high salinity and high organic content that is potentially detrimental to the biological treatment system and affects the treatment efficiency accordingly. The experiment used the integrated bioreactor to reduce much of the organics in mustard tuber wastewater, and found th...

journal_title：Biotechnology and applied biochemistry

authors： Kang W,Chai H,Yang S,Du G,Zhou J,He Q

更新日期：2016-07-01 00:00:00

abstract：:Higher thermostability or acid resistance for fungal α-amylase will help to improve the sugar-making process and cut down the production costs. Here, the thermostability or acid resistance of Rhizopus oryzae α-amylase (ROAmy) was significantly enhanced by site-directed evolution based on multiple sequence alignment (M...

journal_title：Biotechnology and applied biochemistry

authors： Li S,Yang Q,Tang B

更新日期：2020-07-01 00:00:00

abstract：:Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our p...

journal_title：Biotechnology and applied biochemistry

authors： Chen Z,Zhuo W,Wang Y,Ao X,An J

更新日期：2008-06-01 00:00:00

abstract：:Tuberculosis has long been the scourge of humanity, claiming millions of lives. The family of PE_PGRS gene has been attributed to the Mycobacterium tuberculosis pathogenesis over the past few decades. The gene of PE_PGRS family proteins are most often clustered in a region of the genome often as overlapping genes and ...

journal_title：Biotechnology and applied biochemistry

authors： Meena LS

更新日期：2015-03-01 00:00:00

abstract：:The aim of the present study was to find disruption methods that allow fast and reproducible measurement of intracellular recombinant proteins with potential for on-line application. Production of rhSOD (recombinant human superoxide dismutase) by Escherichia coli was used as a model. Three methods of cell disruption, ...

journal_title：Biotechnology and applied biochemistry

authors： Tkac J,Vostiar I,Mandenius CF

更新日期：2004-08-01 00:00:00

abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...

journal_title：Biotechnology and applied biochemistry

authors： Hiragami F,Akiyama J,Koike Y,Kano Y

更新日期：2007-05-01 00:00:00

abstract：:The main metabolic properties of human red blood cells (RBC) overloaded with glucose catabolizing enzymes such as hexokinase and glucose oxidase were evaluated. Human erythrocytes loaded with human hexokinase metabolized 3.1 +/- 0.2 mumol/h/ml RBC of glucose, an amount double that consumed by normal and unloaded cells...

journal_title：Biotechnology and applied biochemistry

authors： Rossi L,Bianchi M,Magnani M

更新日期：1992-04-01 00:00:00

abstract：:Diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase has been isolated previously using classical protein isolation techniques [A. Guranowski and S. Blanquet (1985) J. Biol. Chem. 260, 3542-3547]. A protein A-Sepharose immunoaffinity column was prepared to simplify the purification procedure. The immunoaffini...

journal_title：Biotechnology and applied biochemistry

authors： Avila DM,Kaushal V,Barnes LD

更新日期：1990-06-01 00:00:00

abstract：:c-Jun is a highly oncogenic transcription factor involved in the development of different types of cancer. In the present study we have generated c-Jun-binding-affinity proteins from a phage-displayed library of so-called 'Affibody ligands', developed by combinatorial engineering of a non-immunoglobulin-based scaffold...

journal_title：Biotechnology and applied biochemistry

authors： Lundberg E,Brismar H,Gräslund T

更新日期：2009-01-01 00:00:00

abstract：:The development of lytic bacteriophages as therapeutic products is an attractive alternative to antibiotics. In this study, we evaluated the potential of phage tails for lysing Gram-positive bacteria. Phage P954, a well-characterized temperate staphylococcal phage, was found to adsorb to a large number of Staphylococc...

journal_title：Biotechnology and applied biochemistry

authors： Biddappa AC,Sundarrajan S,Ramalinga AB,Sriram B,Padmanabhan S

更新日期：2012-11-01 00:00:00

abstract：:A kinetic model was developed to describe recombinant human protein disulphide isomerase (rhPDI)-assisted folding of a substrate protein, C125A recombinant human interleukin-2 (C125A rhIL-2). A series of progress curves showing native C125A rhIL-2 formation under different reaction conditions were generated. Non-linea...

journal_title：Biotechnology and applied biochemistry

authors： Du C,Wolfe JL

更新日期：2003-04-01 00:00:00

abstract：:Aspergillus niger and Rhizopus sp. glucoamylases were purified on an affinity chromatography column from commercially available, impure enzyme preparations. Up to 2 mg of glucoamylase protein was bound without leakage to a 1-ml affinity gel column (0.7 X 2.5 cm) possessing a covalently linked acarbose ligand (1 mg aca...

journal_title：Biotechnology and applied biochemistry

authors： Ono K,Smith EE

更新日期：1986-04-01 00:00:00

abstract：:The interpretation of the circular dichroism (c.d.) spectra of proteins to date requires additional secondary structural information of the proteins to be analysed (e.g., X-ray or n.m.r. data). Therefore these methods are inappropriate for a c.d. database whose secondary structures are unknown, as in the case of the m...

journal_title：Biotechnology and applied biochemistry

authors： Fasman GD

更新日期：1993-10-01 00:00:00