An activity transition from NADH dehydrogenase to NADH oxidase during protein denaturation.

abstract：:The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatog...

journal_title：Biotechnology and applied biochemistry

authors： Rigi G,Ghaedmohammadi S,Ahmadian G

更新日期：2019-05-01 00:00:00

abstract：:In the present study, recombinant HCV (hepatitis C virus) core proteins enhanced the immune response elicited by a co-delivered DNA vaccine encoding HCV core and envelope proteins. A mixture of the plasmid pIDKE2 and Co.173, a protein comprising the first 173 amino acids of HCV core, in particular induces a strong hum...

journal_title：Biotechnology and applied biochemistry

authors： Alvarez-Lajonchere L,González M,Alvarez-Obregón JC,Guerra I,Viña A,Acosta-Rivero N,Musacchio A,Morales J,Dueñas-Carrera S

更新日期：2006-04-01 00:00:00

abstract：:Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and ...

journal_title：Biotechnology and applied biochemistry

authors： Karuppaiya P,Tsay HS

更新日期：2020-01-01 00:00:00

abstract：:Cell proliferation is a complex and important event in atherosclerosis, aging and cancer, and is under the control of signalling pathways. These signalling pathways in turn are effected by the presence of a number of chemicals. For this purpose, we have checked the effect of two chemicals on the proliferation of skin ...

journal_title：Biotechnology and applied biochemistry

authors： Onat D,Boscoboinik D,Azzi A,Basaga H

更新日期：1999-06-01 00:00:00

abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...

journal_title：Biotechnology and applied biochemistry

authors： Wen CM,Tseng CS,Cheng CY,Li YK

更新日期：2002-06-01 00:00:00

abstract：:Lys and Leu were generally considered as the key amino acids for brewer's yeast during beer brewing. In the present study, peptide Lys-Leu and a free amino acid (FAA) mixture of Lys and Leu (Lys + Leu) were supplemented in 24 °P wort to examine their effects on physiological activity and fermentation performance of br...

journal_title：Biotechnology and applied biochemistry

authors： Yang H,Zong X,Cui C,Mu L,Zhao H

更新日期：2018-07-01 00:00:00

abstract：:Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...

journal_title：Biotechnology and applied biochemistry

authors： Li Q,Ng WT,Puah SM,Bhaskar RV,Soh LS,MacBeath C,Parakattil P,Green P,Wu JC

更新日期：2014-07-01 00:00:00

abstract：:A deletion htpR mutant of Escherichia coli has been constructed on the basis of site-directed mutagenesis. To this end, the chromosomal allele of htpR gene was substituted by a mutant allele introduced into the cell with a recombinant plasmid. The htpR mutant is characterized by a reduced level of proteolysis and ther...

journal_title：Biotechnology and applied biochemistry

authors： Kiselev VI

更新日期：1988-10-01 00:00:00

abstract：:The urinary C-terminal telopeptide fragment of type II collagen (uCTX-II) has been reported as the efficient blood-based biomarker for osteoarthritis, which affects knees, hands, spine, and hips. This study reports a sensing strategy with antibody-conjugated gold nanoparticles (GNP) on an interdigitated electrode (IDE...

journal_title：Biotechnology and applied biochemistry

authors： Wang S,Su S,Yu C,Gopinath SCB,Yang Z

更新日期：2020-07-04 00:00:00

abstract：:An amylase-producing psychrotroph bacterium was isolated from soil and identified as belonging to the genus Exiguobacterium. A novel cold-adapted α-amylase, Amy SH3, was purified from culture medium of this bacterium using acetone precipitation and DEAE-Sepharose anion-exchange chromatography. The molecular mass of th...

journal_title：Biotechnology and applied biochemistry

authors： Mojallali L,Shahbani Zahiri H,Rajaei S,Akbari Noghabi K,Haghbeen K

更新日期：2014-03-01 00:00:00

abstract：:Three peptides complementary to exposed regions of two low-M(r) phosphotyrosine protein phosphatase isoenzymes and of the acylphosphatase muscle isoenzyme have been synthesized. Each peptide was synthesized on two different types of resins; the peptides were anchored to the resins by amide linkages. The peptide resins...

journal_title：Biotechnology and applied biochemistry

authors： Stefani M,Bucciantini M,Taddei N,Mincione G,Manao G,Ramponi G

更新日期：1994-10-01 00:00:00

abstract：:Amyloglucosidase from Rhizopus mould and beta-glucosidase from sweet almond were employed for the preparation of phenolic and vitamin glycosides of vanillin, N-vanillylnonanamide, DL-dopa, dopamine, curcumin, alpha-tocopherol (vitamin E), pyridoxine (vitamin B(6)), ergocalciferol (vitamin D(2)), thiamin (vitamin B(1))...

journal_title：Biotechnology and applied biochemistry

authors： Charles RE,Ponrasu T,Sivakumar R,Divakar S

更新日期：2009-03-01 00:00:00

abstract：:We tested a variety of liposomes for parameters such as DNA binding capacity and DNase I protection of incorporated and attached DNA to elucidate their use as vehicles for DNA transfer into cells and animals. The results were compared to other potential DNA vehicles, empty viral capsids, and nanoparticles. Maximal bin...

journal_title：Biotechnology and applied biochemistry

authors： Bertling WM,Gareis M,Paspaleeva V,Zimmer A,Kreuter J,Nürnberg E,Harrer P

更新日期：1991-06-01 00:00:00

abstract：:Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H2 , organic acids, ethanol, and cellulase has been explo...

journal_title：Biotechnology and applied biochemistry

authors： Kumar P,Maharjan A,Jun HB,Kim BS

更新日期：2019-03-01 00:00:00

abstract：:Amylin has been implicated in type II diabetes because of its inherent property to misfold into toxic aggregates. Although it has been shown that amylin interacts with cell membranes, no study to date has monitored the association process using a direct approach. The present study uses confocal microscopy to identify ...

journal_title：Biotechnology and applied biochemistry

authors： Pillay K,Govender P

更新日期：2013-07-01 00:00:00

abstract：:Recently, hPNAS-4 [human PNAS-4; also known as C1orf121 (Genbank accession number NP-057160)] was reported to be a novel pro-apoptotic protein in mammalian cells. However, at present there is little information about hPNAS-4 and its molecular mechanisms. In our present studies, the hPNAS-4 gene was first cloned into t...

journal_title：Biotechnology and applied biochemistry

authors： Deng H,Jiang Q,Liang S,Yan F,Hou S,Qian Z,Li J,Wen Y,Yang J,Wei Y

更新日期：2010-02-11 00:00:00

abstract：:The conformation of a protein refers to the three-dimensional arrangement of its constituent atoms. Since expression of the biological activity of a protein depends on its conformation, it is clear that full characterization of a protein involves an understanding of its three-dimensional structure. This review outline...

journal_title：Biotechnology and applied biochemistry

authors： Price NC

更新日期：2000-02-01 00:00:00

abstract：:Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcinoembryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in th...

journal_title：Biotechnology and applied biochemistry

authors： Mao Q,Liu X,Chen C,Ye J,Liang H,Li B,Sun X,Sun L,Sun Y,Mu X,Li L

更新日期：2018-11-01 00:00:00

abstract：:Oxidative DNA damage is involved in mutagenesis, carcinogenesis, aging, radiation effects and also in the action of several anticancer drugs. Accumulated evidence indicates that iron may play an important role in these processes. The conversion of the closed circular double-stranded supercoiled plasmid pcDNA3 into the...

journal_title：Biotechnology and applied biochemistry

authors： El-Sayed IH,El-Masry SA

更新日期：2003-12-01 00:00:00

abstract：:The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...

journal_title：Biotechnology and applied biochemistry

authors： Son HJ,Heo MS,Kim YG,Lee SJ

更新日期：2001-02-01 00:00:00

abstract：:Mouse band-3 crosslinked carrier erythrocytes have been prepared. [125I]Carbonic anhydrase (CA) has been encapsulated into mouse erythrocytes. Then, loaded erythrocytes were labelled with 51Cr. Eventually, these doubly labelled cells were crosslinked with band-3 crosslinking reagents. [125I]CA was shown to have cytoso...

journal_title：Biotechnology and applied biochemistry

authors： Jordán JA,Alvarez FJ,Tejedor MC,Díez JC

更新日期：1999-02-01 00:00:00

abstract：:We have analysed the status and transcriptional activity of the bovine papillomavirus-I (complete BPV-I genome)-based expression vector pCES in CI27i-cell-line-derived 3TI cells used for the industrial production of recombinant human erythropoietin (rhuEpo). Complete tandem head-to-tail integration of about 600 vector...

journal_title：Biotechnology and applied biochemistry

authors： Fibi MR,Kunz J,Weimer T,Schulz R,Teifke P,Grzeschik KH,Johannsen R,Zettlmeissl G

更新日期：1996-02-01 00:00:00

abstract：:High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...

journal_title：Biotechnology and applied biochemistry

authors： Eriksson C,Schwenk JM,Sjöberg A,Hober S

更新日期：2010-06-09 00:00:00

abstract：:A three-layered simulation methodology is described that rapidly evaluates biomanufacturing process options. In each layer, inferior options are screened out, while more promising candidates are evaluated further in the subsequent, more refined layer, which uses more rigorous models that require more data from time-co...

journal_title：Biotechnology and applied biochemistry

authors： Chhatre S,Francis R,O'Donovan K,Titchener-Hooker NJ,Newcombe AR,Keshavarz-Moore E

更新日期：2007-10-01 00:00:00

abstract：:Feeding a basal diet free of vitamins E and C to weanling male rats for 8 months resulted in biochemical changes characteristic of vitamin E deficiency. These included increased liver thiobarbituric acid values; decreased blood GSH levels, plasma vitamin E levels, and glutathione peroxidase activities; and increased a...

journal_title：Biotechnology and applied biochemistry

authors： Chen LH,Thacker RR

更新日期：1986-02-01 00:00:00

abstract：:The introduction of different methodologies for the construction and screening of complex protein libraries has provided powerful means in protein engineering for the development of molecules with desired traits. A challenge faced in many situations is to adapt a given methodology for efficient and rapid identificatio...

journal_title：Biotechnology and applied biochemistry

authors： Löfdahl PA,Nygren PA

更新日期：2010-03-05 00:00:00

abstract：:To improve the thermostability of (R)-selective amine transaminase from Aspergillus terreus (AT-ATA), we used computer software Disulfide by Design and Modelling of Disulfide Bonds in Proteins to identify mutation sites where the disulfide bonds were most likely to form. We obtained three stabilized mutants (N25C-A28C...

journal_title：Biotechnology and applied biochemistry

authors： Xie DF,Fang H,Mei JQ,Gong JY,Wang HP,Shen XY,Huang J,Mei LH

更新日期：2018-03-01 00:00:00

abstract：:The α-amylase from soya bean seeds was purified by affinity precipitation, resulting in approx. 20-fold purification with approx. 84% recovery. The purified α-amylase had an optimum pH of 5.5, optimum temperature of 75 °C, Arrhenius energy of activation of 6.03 kcal/mol (1 kcal≈4.184 kJ) and a Km of 2.427 mg/ml (starc...

journal_title：Biotechnology and applied biochemistry

authors： Prakash O,Jaiswal N

更新日期：2010-12-01 00:00:00

abstract：:HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...

journal_title：Biotechnology and applied biochemistry

authors： Vázquez-Blomquist D,González S,Duarte CA

更新日期：2002-12-01 00:00:00

abstract：:Cholesterol esterase modified with polyethylene glycol was able to dissolve in some highly hydrophobic solvents such as benzene and toluene, and catalyze the synthesis of cholesterol linoleate with time dependency in the reverse of the usual reaction in aqueous solvents. Enzymatic cholesterol linoleate synthesis follo...

journal_title：Biotechnology and applied biochemistry

authors： Mori S,Nakata Y,Endo H

更新日期：1992-06-01 00:00:00