Abstract:
:Oxidative DNA damage is involved in mutagenesis, carcinogenesis, aging, radiation effects and also in the action of several anticancer drugs. Accumulated evidence indicates that iron may play an important role in these processes. The conversion of the closed circular double-stranded supercoiled plasmid pcDNA3 into the nicked circular and linear forms was used to investigate DNA nicking induced by the reactions of an iron complex of 2-methylaminopyridine (L), which exhibited a pronounced superoxide dismutase-mimetic activity and antitumour activity with H2O2. Hence the dose-response curve for the [FeL2Cl2]Cl.H2O-mediated H2O2-dependent DNA nicking was studied. For a fixed concentration of [FeL2Cl2]Cl.H2O (25 microM), the concentration of H2O2 producing a maximum extent of DNA nicking was 100 microM. The effects of these two constituents are synergistic. The biological antioxidants such as glycerol, sodium azide and superoxide dismutase significantly inhibited DNA breakage induced by [FeL2Cl2]Cl.H2O and hydrogen peroxide.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
El-Sayed IH,El-Masry SAdoi
10.1042/BA20030047subject
Has Abstractpub_date
2003-12-01 00:00:00pages
253-6issue
Pt 3eissn
0885-4513issn
1470-8744pii
BA20030047journal_volume
38pub_type
杂志文章abstract::We studied, for the first time, characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of enzyme conformational stability and structural behaviour induced by temperature as a function of pH using enzymic assays, differential scanning calorimetry, fluo...
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