Abstract：
:The results from molecular mass determinations and amino acid analyses for prostate-specific antigen (PSA), alpha 1-antichymotrypsin (ACT) and PSA-ACT complexed in vitro are reported. Molecular masses for two separate PSA-ACT lots were determined by matrix-assisted laser desorption ionization mass spectroscopy (MALDI-MS) coupled to a time-of-flight (TOF) detector. Interestingly, both PSA-ACT lots contained two predominant protein species: 78,095 +/- 138 Da (approx. 67% of total protein) and 82,519 +/- 104 Da. Because a heterogneous population was observed and the masses were less than expected on the basis of less sensitive techniques, molecular masses for the individual PSA and ACT components were determined. As expected, PSA possessed a single molecular mass (27,755.8 Da). Each ACT raw material lot, however, also contained two predominant species: 55,106 +/- 111 and 51,414 +/- 32 Da. To assess the amino acid composition, each PSA, ACT and PSA-ACT lot was subjected to acid hydrolysis for 24, 48 and 72 h followed by amino acid analysis. Experimental results, expressed as mole percentages for each measurable residue and the number of residues per mole of protein, were compared with both the predicted and previously published values and were as expected. These more accurate molecular mass values for ACT and purified PSA-ACT complex should be considered in preparing and characterizing international standard preparations.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Bedzyk WD,Larsen B,Gutteridge S,Ballas RAsubject
Has Abstractpub_date
1998-06-01 00:00:00pages
249-57issue
3eissn
0885-4513issn
1470-8744journal_volume
27pub_type
杂志文章abstract：:The sialidase gene from Brevibacterium casei was cloned in pET28a and overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3). The histidine-tagged sialidase protein was purified and characterized from the crude cell extracts of isopropyl-β-d-thiogalactopyranoside-induced cells using Ni-NTA agarose c...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1475
更新日期：2017-03-01 00:00:00
abstract：:In the present study, the effect of the neuroleptics chlorpromazine (2-chloro-N,N-dimethyl-10H-phenothiazine-10-propanamine) and trifluoperazine {10-[3-(4-methylpiperazin-l-yl)-propyl]-2-trifluoromethyl)-10-H- [phenothiazine}-10H-phenothiazine¿ on the EPR-spectroscopic properties of ferrous human adult nitrosylated ha...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcinoembryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in th...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1663
更新日期：2018-11-01 00:00:00
abstract：:This review article focuses on the use of the methylotrophic yeast Pichia pastoris as a recombinant protein-expression system. P. pastoris is a useful system for the expression of milligram-to-gram quantities of a protein, which can be scaled up to fermentation to meet greater demands. Compared with mammalian cells, P...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1999-10-01 00:00:00
abstract：:Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin inj...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1495
更新日期：2017-05-01 00:00:00
abstract：:Immobilized metal affinity chromatography (IMAC) is widely used for the production of recombinant proteins for a variety of applications; however, a number of challenges are typically encountered by researchers depending on the properties of the specific proteins in question. Here, we describe technical issues we have...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.24
更新日期：2011-05-01 00:00:00
abstract：:[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:In the present study, recombinant HCV (hepatitis C virus) core proteins enhanced the immune response elicited by a co-delivered DNA vaccine encoding HCV core and envelope proteins. A mixture of the plasmid pIDKE2 and Co.173, a protein comprising the first 173 amino acids of HCV core, in particular induces a strong hum...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20050202
更新日期：2006-04-01 00:00:00
abstract：:The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a plant species known for its production of TIAs (terpenoid indole alkaloids), many of which are pharmaceutically important. Ajmalicine and serpentine are prescribed for the treatment of hypertension, whereas the bisindoles vinblastine, vincristine and 3',...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1042/BA20080239
更新日期：2009-04-01 00:00:00
abstract：:The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferul...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:Succinylcholine apnea happens in cases of null butyrylcholinesterase activity after administration of preintubation succinylcholine. So far, there is no such popular test that can rapidly screen null butyrylcholinesterase activity from plasma. Development of a novel method for rapid screening of null butyrylcholineste...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1252
更新日期：2015-03-01 00:00:00
abstract：:Melamine adulteration of food is a public health concern. It has been seen that melamine causes disease in many organs. Melamine-induced kidney disease is a well-recognized clinicopathological entity. Inflammation is thought to be important in melamine-induced pathology. Melamine is expected to bind with albumin becau...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1512
更新日期：2017-07-01 00:00:00
abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20020001
更新日期：2002-06-01 00:00:00
abstract：:This study was designed to investigate the protective effects of Acanthus ilicifolius alkaloid A [4-hydroxy-2-benzoxazolone (HBOA)] and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride (CCl4 )-induced liver fibrosis in rats. Spr...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1292
更新日期：2015-07-01 00:00:00
abstract：:Chronic myelogenous leukemia (CML) is a hematologic malignancy characterized by excessive growth of myeloid cells and their progenitors. The proportion of spectrin dimers compared to tetramers extracted from membranes at 4 degrees C, under low ionic strength conditions, increased in CML erythrocytes. These also displa...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1990-10-01 00:00:00
abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.2006
更新日期：2020-08-15 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1002/bab.1056
更新日期：2013-01-01 00:00:00
abstract：:Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new ti...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1990-10-01 00:00:00
abstract：:We evaluated a new protocol for measurement of cyclosporine A (CsA) 2 H after dose (C2) on the V-Twin® analyzer. Imprecision, recovery, and linearity were determined using CsA-spiked blood pools. Accuracy was evaluated using specimens from renal, cardiac, and liver transplant patients, and results were compared with t...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.43
更新日期：2011-11-01 00:00:00
abstract：:The hydrolysis kinetics of native and denatured haemoglobin, using pepsin immobilized on aluminium oxide, was studied in order to produce hydrolysates containing bioactive peptides. Pepsin was immobilized on acidic alumina and on 2-ethanolamine- O -phosphate (2-EAOP)-modified acidic alumina. Surface charge of the two ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20030131
更新日期：2004-04-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1810
更新日期：2020-01-01 00:00:00
abstract：:2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1042/BA20090253
更新日期：2010-03-12 00:00:00
abstract：:Cardiolipin, the primary lipid hapten in the antigen suspension used for the detection of antitreponemal antibodies in the sera of syphilitic patients, was successfully coupled to glucose oxidase, peroxidase, and some other enzymes using different crosslinking agents. These complexes were used to replace the pure unco...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Penicillium ochrochloron Biourge, which was isolated from rotten sweet potato, can produce plant cell wall-degrading enzymes (PCWDEs) with high viscosity reducing capability for ethanol production using fresh sweet potato tubers as feedstock. The enzyme preparation was characterized by a broad enzyme spectrum includin...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1190
更新日期：2014-07-01 00:00:00
abstract：:It is now a well-known fact that alpha-CD (alpha-cyclodextrin) and Ca(2+) are two important folding agents in the re-activation and refolding processes of many denatured enzyme solutions. Bacillus sp. alpha-amylase was extensively denatured in a 6 M guanidinium chloride solution overnight. Under suitable renaturation ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20040070
更新日期：2005-04-01 00:00:00
abstract：:The plant polymer lignin is the greatest source of aromatic chemical structures on earth. Hence, the chemically diverse lignin monomers are valuable raw materials for fine chemicals, materials synthesis, and food and flavor industries. However, extensive use of this natural resource is hampered by the large number of ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1222
更新日期：2014-11-01 00:00:00
abstract：:Neuroblastoma (NB) is an embryonic solid tumor derived from precursor cells of the sympathetic nervous system, and accounts for 11% of childhood cancers and around 15% of cancer deaths in children. SUMOylation and deSUMOylation are dynamic mechanisms regulating a spectrum of protein activities. The SUMO proteases (SEN...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1375
更新日期：2016-05-01 00:00:00
