A kinetic study of bovine haemoglobin hydrolysis by pepsin immobilized on a functionalized alumina to prepare hydrolysates containing bioactive peptides.

abstract：:The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...

journal_title：Biotechnology and applied biochemistry

authors： Son HJ,Heo MS,Kim YG,Lee SJ

更新日期：2001-02-01 00:00:00

abstract：:Metal prostheses of artificial joints undergo wear, producing numerous metal particles and ions, such as Cr3+ . Cr3+ is considered a key factor leading to aseptic loosening. Many studies focus on the effect of Cr3+ on osteoblasts; however, little is known about the effect of Cr3+ on the B-cell maturation antigen (BCMA...

journal_title：Biotechnology and applied biochemistry

authors： Li DH,Yang Q,Zhou JS,Zhang ZW,Miao MY,Yang SS,Xu WD

更新日期：2017-09-01 00:00:00

abstract：:The objective of this work is to develop structured, segregated stochastic models for bioprocesses using time-series flow cytometric (FC) data. To this end, mammalian CHO cells were grown in both batch and fed-batch cultures, and their viable cell numbers (VCDs), monoclonal antibody (MAb), cell cycle phases, mitochond...

journal_title：Biotechnology and applied biochemistry

authors： Shirsat N,Avesh M,English NJ,Glennon B,Al-Rubeai M

更新日期：2013-09-01 00:00:00

abstract：:To reduce the degradation pathway of rhIFN-gamma (recombinant human interferon-gamma), the susceptibility against oxidative stress during fermentation in Escherichia coli cells and in purification process were investigated. Fermentations of recombinant E. coli were performed at 5, 30 and 60% DO (dissolved oxygen) conc...

journal_title：Biotechnology and applied biochemistry

authors： Mohammadian-Mosaabadi J,Naderi-Manesh H,Maghsoudi N,Khalilzadeh R,Shojaosadati SA,Ebrahimi M

更新日期：2005-02-01 00:00:00

abstract：:Talpha1 (thymosin alpha 1) is important in treating immunodeficiency and other diseases. In order to study the feasibility of expressing Talpha1 in plants, as the first attempt, we designed and synthesized the Talpha1 gene according to the plant codon usage preference and constructed the 4xTalpha1 concatemer (four cop...

journal_title：Biotechnology and applied biochemistry

authors： Chen Y,Zhao L,Shen G,Cui L,Ren W,Zhang H,Qian H,Tang K

更新日期：2008-01-01 00:00:00

abstract：:Oxidative DNA damage is involved in mutagenesis, carcinogenesis, aging, radiation effects and also in the action of several anticancer drugs. Accumulated evidence indicates that iron may play an important role in these processes. The conversion of the closed circular double-stranded supercoiled plasmid pcDNA3 into the...

journal_title：Biotechnology and applied biochemistry

authors： El-Sayed IH,El-Masry SA

更新日期：2003-12-01 00:00:00

abstract：:Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, the molecular cause of the disease is poorly elucidated. In the present study, we primarily found that translationally controlled tumor protein (TCTP) was overexpressed in human OS tissues and cell lines. To investigate t...

journal_title：Biotechnology and applied biochemistry

authors： Shen JH,Qu CB,Chu HK,Cui MY,Wang YL,Sun YX,Song YD,Li G,Shi FJ

更新日期：2016-01-01 00:00:00

abstract：:The application of the hydroperoxidase activity of immobilized lipoxygenase to xenobiotic metabolization is reported in this work. Soya bean lipoxygenase has been immobilized by covalent coupling to oxirane acrylic beads. This immobilized system has been applied to the oxidation of chlorpromazine (CPZ), a phenothiazin...

journal_title：Biotechnology and applied biochemistry

authors： Santano E,Pinto Mdel C,Macías P

更新日期：2002-10-01 00:00:00

abstract：:2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a ...

journal_title：Biotechnology and applied biochemistry

authors： Lautié E,Fliniaux MA,Villarreal ML

更新日期：2010-03-12 00:00:00

abstract：:In this study, various constructs and hosts were used to produce high levels of cecropin B2 (cecB2). To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin-binding domain and an intein self-cleavage motif in front of cecropin B2, without...

journal_title：Biotechnology and applied biochemistry

authors： Fang YT,Lai WS,Liu JH,Liu YC

更新日期：2019-03-01 00:00:00

abstract：:HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...

journal_title：Biotechnology and applied biochemistry

authors： Vázquez-Blomquist D,González S,Duarte CA

更新日期：2002-12-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) still represents a major problem to the swine industry worldwide, causing high mortality rates in infected animals. Virus-like particles (VLPs) have gained attention for vaccine development, serving both as scaffolds for epitope expression and immune response enhancers. The commercial ...

journal_title：Biotechnology and applied biochemistry

authors： Aguilera BE,Chávez-Calvillo G,Elizondo-Quiroga D,Jimenez-García MN,Carrillo-Tripp M,Silva-Rosales L,Hernández-Gutiérrez R,Gutiérrez-Ortega A

更新日期：2017-05-01 00:00:00

abstract：:The conformation of a protein refers to the three-dimensional arrangement of its constituent atoms. Since expression of the biological activity of a protein depends on its conformation, it is clear that full characterization of a protein involves an understanding of its three-dimensional structure. This review outline...

journal_title：Biotechnology and applied biochemistry

authors： Price NC

更新日期：2000-02-01 00:00:00

abstract：:An extremely thermostable UDP-GlcNAc pyrophosphorylase has been purified from Thermus caldophilus GK24 by chromatographic methods including ion-exchange, hydrophobic interaction, and affinity chromatographies. The specific activity of the enzyme was enriched 41.8-fold, with a recovery of 2%. The molecular mass of the ...

journal_title：Biotechnology and applied biochemistry

authors： Kim JS,Koh S,Shin HJ,Lee DS,Lee SY

更新日期：1999-02-01 00:00:00

abstract：:Mouse and human erythrocytes are inherently different with respect to slow dialysis encapsulation used in preparing carrier erythrocytes. Fluorescein isothiocyanate (FITC)-dextran was added to five different stages of the encapsulation process to discern when endocytic vesiculation occurred. Mouse erythrocytes were mu...

journal_title：Biotechnology and applied biochemistry

authors： DeLoach JR,Droleskey RE

更新日期：1993-08-01 00:00:00

abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...

journal_title：Biotechnology and applied biochemistry

authors： Ramström M,Zuberovic A,Grönwall C,Hanrieder J,Bergquist J,Hober S

更新日期：2009-02-01 00:00:00

abstract：:A new α-neoagarobiose hydrolase (NABH) called AgaWH117 was cloned from Agarivorans gilvus WH0801. The gene encoding this hydrolase consists of 1,086 bp and encodes a protein containing 361 amino acids. This new NABH showed 74% amino acid sequence identity with other known NABHs. The molecular mass of the recombinant A...

journal_title：Biotechnology and applied biochemistry

authors： Liu N,Yang M,Mao X,Mu B,Wei D

更新日期：2016-03-01 00:00:00

abstract：:Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (E...

journal_title：Biotechnology and applied biochemistry

authors： Lau MF,Chua KH,Sabaratnam V,Kuppusamy UR

更新日期：2020-08-28 00:00:00

abstract：:Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin-streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplification is overamplific...

journal_title：Biotechnology and applied biochemistry

authors： Shao K,Shi X,Zhu X,Cui L,Shao Q,Ma D

更新日期：2017-03-01 00:00:00

abstract：:Mouse band-3 crosslinked carrier erythrocytes have been prepared. [125I]Carbonic anhydrase (CA) has been encapsulated into mouse erythrocytes. Then, loaded erythrocytes were labelled with 51Cr. Eventually, these doubly labelled cells were crosslinked with band-3 crosslinking reagents. [125I]CA was shown to have cytoso...

journal_title：Biotechnology and applied biochemistry

authors： Jordán JA,Alvarez FJ,Tejedor MC,Díez JC

更新日期：1999-02-01 00:00:00

abstract：:Fermentation of herb Polygonum hydropiper L. (PHL) and cassava pulp (CP) for feed additive production with simultaneous flavonoid dissolution was investigated, and a two-stage response surface methodology (RSM) based on Plackett-Burman factorial design (PB design) was used to optimize the flavonoid dissolution and pro...

journal_title：Biotechnology and applied biochemistry

authors： Song ZT,Zhu MJ

更新日期：2017-03-01 00:00:00

abstract：:Cardiolipin, the primary lipid hapten in the antigen suspension used for the detection of antitreponemal antibodies in the sera of syphilitic patients, was successfully coupled to glucose oxidase, peroxidase, and some other enzymes using different crosslinking agents. These complexes were used to replace the pure unco...

journal_title：Biotechnology and applied biochemistry

authors： Ngeh-Ngwainbi J,Kuan SS,Steinman F,Guilbault GG

更新日期：1986-12-01 00:00:00

abstract：:A new system equipped with a computer-controlled multiple activity analyzer has been developed for the efficient purification of multiple enzymes. The system consists of the following units: conventional enzyme fractionation system with a peristaltic pump, liquid chromatographic column, fraction collector, and uv moni...

journal_title：Biotechnology and applied biochemistry

authors： Tokushige M,Sasaki S

更新日期：1986-12-01 00:00:00

abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...

journal_title：Biotechnology and applied biochemistry

authors： Qiu C,Wang X,Zhang X,Li Z,Zhou Y,Kang J

更新日期：2020-08-15 00:00:00

abstract：:Production of His-BbST [hexahistidine-tagged BbST (bubaline somatotropin)] by auto-induction in high-density shake-flask cultures coupled with a single-step, on-column purification and refolding strategy is described here. To optimize expression of BbST, different media and expression conditions were tested. The highe...

journal_title：Biotechnology and applied biochemistry

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2007-05-01 00:00:00

abstract：:The therapeutic potential of microRNA-21 (miR-21) small-molecule inhibitors has been of particular interest to medicinal chemists. Moreover, the development of more facile screening methods is lacking. In the present study, two potential screening strategies for miR-21 small-molecule inhibitor including the stem-loop ...

journal_title：Biotechnology and applied biochemistry

authors： Hei YY,Guo YX,Jiang CS,Wang S,Lu SM,Zhang SQ

更新日期：2019-09-01 00:00:00

abstract：:Auricularia polytricha belonging to Basidiomycota has the ability to degrade lignocellulose. However, there has been no resource in public databases examining the transcriptome of A. polytricha. In this study, high-throughput sequencing platform BGISEQ-500 was used to generate large amount of transcript sequences from...

journal_title：Biotechnology and applied biochemistry

authors： Jiang S,Zheng X,Li L

更新日期：2020-08-12 00:00:00

abstract：:The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as...

journal_title：Biotechnology and applied biochemistry

authors： Cannio R,de Pascale D,Rossi M,Bartolucci S

更新日期：1994-04-01 00:00:00

abstract：:A simple and cost-effective method is described to sequence double-stranded DNA (< 100 ng) using a very small amount of fluorescent sequencing mix (one-tenth of manufacturer-recommended amount). The modification has significant advantages over conventional protocols and can be used to sequence, using a single sequenci...

journal_title：Biotechnology and applied biochemistry

authors： Beg OU,Holt RG

更新日期：1997-08-01 00:00:00

abstract：:[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of ...

journal_title：Biotechnology and applied biochemistry

authors： Abiko T,Shishido H,Sekino H

更新日期：1986-04-01 00:00:00