The dual luciferase reporter system and RT-qPCR strategies for screening of MicroRNA-21 small-molecule inhibitors.

abstract：:As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...

journal_title：Biotechnology and applied biochemistry

authors： Hasegawa M,Kaku T,Kuroda M,Ise N,Kitano H

更新日期：1992-02-01 00:00:00

abstract：:Colloidal carriers based on silica (Si) matrices are an innovative approach within the context of therapeutic drug delivery systems. These carriers are emerging as a great promise for diagnosis and treatment of a wide range of injuries, particularly in cancer and infectious diseases. In addition, bioencapsulation for ...

journal_title：Biotechnology and applied biochemistry

authors： Andreani T,Silva AM,Souto EB

更新日期：2015-11-01 00:00:00

abstract：:Amylin has been implicated in type II diabetes because of its inherent property to misfold into toxic aggregates. Although it has been shown that amylin interacts with cell membranes, no study to date has monitored the association process using a direct approach. The present study uses confocal microscopy to identify ...

journal_title：Biotechnology and applied biochemistry

authors： Pillay K,Govender P

更新日期：2013-07-01 00:00:00

abstract：:Talpha1 (thymosin alpha 1) is important in treating immunodeficiency and other diseases. In order to study the feasibility of expressing Talpha1 in plants, as the first attempt, we designed and synthesized the Talpha1 gene according to the plant codon usage preference and constructed the 4xTalpha1 concatemer (four cop...

journal_title：Biotechnology and applied biochemistry

authors： Chen Y,Zhao L,Shen G,Cui L,Ren W,Zhang H,Qian H,Tang K

更新日期：2008-01-01 00:00:00

abstract：:Most insulin-dependent diabetes mellitus patients gen-erate conformational autoantibodies to the islet-cell 65-kDa variant of human glutamate decarboxylase (GAD65), and several immunochemical tests for the early detection of type-1 diabetes rely on GAD65 antibody (GADA) assessment using properly folded recombinant GAD...

journal_title：Biotechnology and applied biochemistry

authors： Santos J,Antón EA,Buslje CM,Valdez SN,Villanueva AL,Sica M,Iacono R,Maffia P,Poskus E,Ermácora MR

更新日期：2000-06-01 00:00:00

abstract：:Naproxen and bezafibrate fall into the category of pharmaceuticals that have been widely detected in the aquatic environment, and one of the major sources is the effluent discharge from wastewater treatment plants. This study investigated the sorption and degradation kinetics of naproxen and bezafibrate in the presenc...

journal_title：Biotechnology and applied biochemistry

authors： Tang Y,Li XM,Xu ZC,Guo QW,Hong CY,Bing YX

更新日期：2014-05-01 00:00:00

abstract：:This study was designed to investigate the protective effects of Acanthus ilicifolius alkaloid A [4-hydroxy-2-benzoxazolone (HBOA)] and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride (CCl4 )-induced liver fibrosis in rats. Spr...

journal_title：Biotechnology and applied biochemistry

authors： Wai KK,Liang Y,Zhou L,Cai L,Liang C,Liu L,Lin X,Wu H,Lin J

更新日期：2015-07-01 00:00:00

abstract：:Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in de...

journal_title：Biotechnology and applied biochemistry

authors： Chen L,Guo S,Wu L,Hao C,Xu W,Zhang J

更新日期：2015-05-01 00:00:00

abstract：:The production of rabies virus from baby hamster kidney-Ankara66 (BHK-An(66), HUKUK 99050302) monolayer cells was examinedin a packed-bed reactor containing non-woven polyester fabric (NWPF)discs. At first, growth characteristics of the cells were determined instatic culture. The suspension culture studies were realiz...

journal_title：Biotechnology and applied biochemistry

authors： Gümüşderelioğlu M,Aslankaraoğlu E,Gürhan SI

更新日期：2001-06-01 00:00:00

abstract：:HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...

journal_title：Biotechnology and applied biochemistry

authors： Vázquez-Blomquist D,González S,Duarte CA

更新日期：2002-12-01 00:00:00

abstract：:Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and ...

journal_title：Biotechnology and applied biochemistry

authors： Karuppaiya P,Tsay HS

更新日期：2020-01-01 00:00:00

abstract：:The immobilization of F(0)F(1)-ATPase in uniform orientation is reported. The biotinylated and histidine-tagged subunits of the bacterial F(0)F(1)-ATPase complex were used for immobilization of the complex on artificial semi-permeable membranes resulting in 88+/-7.8 and 72+/-5.2% coupling of the enzymes. The immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2004-06-01 00:00:00

abstract：:The dynamic gassing-out method was used to assess the effect of stirrer speed, air flow rate and alginate concentration on the overall volumetric oxygen mass transfer coefficient (K(L)a) in a bench-top stirred fermenter equipped with two Rushton-type turbines. As the alginate concentration in the medium was increased ...

journal_title：Biotechnology and applied biochemistry

authors： Parente E,Piraino P,Fidaleo M,Moresi M

更新日期：2004-10-01 00:00:00

abstract：:The use of glutaraldehyde (GTA) for cross-linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross-linking agent for tissue implants, we have evaluated a number of cross-linking procedures shown previousl...

journal_title：Biotechnology and applied biochemistry

authors： Simmons DM,Kearney JN

更新日期：1993-02-01 00:00:00

abstract：:Restoration of insulin secretion by transplantation of isolated islets is a treatment for type Ι diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate e...

journal_title：Biotechnology and applied biochemistry

authors： Li N,Zhang Y,Xiu Z,Wang Y,Chen L,Wang S,Li S,Guo X,Ma X

更新日期：2015-07-01 00:00:00

abstract：:Tissue engineering has been conducted in the study of cardiovascular grafts for many years. Many obstacles have been overcome in this rapidly changing field, but one difficulty has remained until now: the large number of endothelial cells (ECs) needed for seeding the inner layer of bypass graft. Recent advances in end...

journal_title：Biotechnology and applied biochemistry

authors： Punshon G,Vara DS,Sales KM,Seifalian AM

更新日期：2011-09-01 00:00:00

abstract：:Monoclonal antibodies specific to human prolactin were generated by an improved hybridoma technique. Following immunization with hormone conjugated with hemocyanin and cell fusion, hybrid cells were first cultured in a semisolid medium containing methylcellulose and later transferred to liquid medium for subculture. O...

journal_title：Biotechnology and applied biochemistry

authors： Lee CY,Chow SN,Coleman P,DeTuri MP

更新日期：1987-02-01 00:00:00

abstract：:Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcinoembryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in th...

journal_title：Biotechnology and applied biochemistry

authors： Mao Q,Liu X,Chen C,Ye J,Liang H,Li B,Sun X,Sun L,Sun Y,Mu X,Li L

更新日期：2018-11-01 00:00:00

abstract：:The aspartic proteinase gene of Mucor pusillus rennin expressed in Pichia pastoris was characterized in terms of structural and conformational stability induced by temperature. This enzyme is 12% glycosylated, with a similar specific activity to the native fungal enzyme. The secondary structure determined by CD is mai...

journal_title：Biotechnology and applied biochemistry

authors： Beldarraín A,Acosta N,Montesinos R,Mata M,Cremata J

更新日期：2000-04-01 00:00:00

abstract：:Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, the molecular cause of the disease is poorly elucidated. In the present study, we primarily found that translationally controlled tumor protein (TCTP) was overexpressed in human OS tissues and cell lines. To investigate t...

journal_title：Biotechnology and applied biochemistry

authors： Shen JH,Qu CB,Chu HK,Cui MY,Wang YL,Sun YX,Song YD,Li G,Shi FJ

更新日期：2016-01-01 00:00:00

abstract：:A sensitive sandwich enzyme immunoassay (e.i.a.) for serrapeptase (TSP), an orally available anti-inflammatory proteinase, was established using affinity-purified anti-TSP rabbit IgG and its Fab' fragment conjugated with horseradish peroxidase as the first and the second antibodies respectively. TSP in the plasma was ...

journal_title：Biotechnology and applied biochemistry

authors： Moriya N,Nakata M,Nakamura M,Takaoka M,Iwasa S,Kato K,Kakinuma A

更新日期：1994-08-01 00:00:00

abstract：:The results from molecular mass determinations and amino acid analyses for prostate-specific antigen (PSA), alpha 1-antichymotrypsin (ACT) and PSA-ACT complexed in vitro are reported. Molecular masses for two separate PSA-ACT lots were determined by matrix-assisted laser desorption ionization mass spectroscopy (MALDI-...

journal_title：Biotechnology and applied biochemistry

authors： Bedzyk WD,Larsen B,Gutteridge S,Ballas RA

更新日期：1998-06-01 00:00:00

abstract：:Penicillium ochrochloron Biourge, which was isolated from rotten sweet potato, can produce plant cell wall-degrading enzymes (PCWDEs) with high viscosity reducing capability for ethanol production using fresh sweet potato tubers as feedstock. The enzyme preparation was characterized by a broad enzyme spectrum includin...

journal_title：Biotechnology and applied biochemistry

authors： Huang Y,Jin Y,Shen W,Fang Y,Zhang G,Zhao H

更新日期：2014-07-01 00:00:00

abstract：:The protective antigen (PA) component of anthrax toxin mediates delivery of either lethal factor (LF) or oedema factor into the cytosol of mammalian cells. The N-terminal domain of LF(1-254) (amino acids 1-254 of LF) binds to PA and, when fused to heterologous proteins, delivers such proteins into the cytosol. In the ...

journal_title：Biotechnology and applied biochemistry

authors： Sharma M,Khanna H,Arora N,Singh Y

更新日期：2000-08-01 00:00:00

abstract：:Human urokinase was immobilized on an ethylene vinyl acetate copolymer surface. Soluble urokinase showed its maximum activity at pH 8.5, while the immobilized enzyme was most active at pH 9.0. Apparently, the shift in optimal pH was due to the polyanionic nature of the carrier surface on which the enzyme was immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Yabushita Y

更新日期：1988-06-01 00:00:00

abstract：:Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H2 , organic acids, ethanol, and cellulase has been explo...

journal_title：Biotechnology and applied biochemistry

authors： Kumar P,Maharjan A,Jun HB,Kim BS

更新日期：2019-03-01 00:00:00

abstract：:In order to impart an ability for receptor-independent transmembrane transfer to water-soluble proteins, it has been suggested that they be hydrophobized by lipid groups (fatty acids, etc.). To this end, systems of reversed micelles of surfactants in organic solvents were used as reaction media for protein modificatio...

journal_title：Biotechnology and applied biochemistry

authors： Alakhov VYu,Kabanov AV,Batrakova EV,Koromyslova IA,Levashov AV,Severin ES

更新日期：1990-02-01 00:00:00

abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...

journal_title：Biotechnology and applied biochemistry

authors： Qiu C,Wang X,Zhang X,Li Z,Zhou Y,Kang J

更新日期：2020-08-15 00:00:00

abstract：:Cholesterol esterase modified with polyethylene glycol was able to dissolve in some highly hydrophobic solvents such as benzene and toluene, and catalyze the synthesis of cholesterol linoleate with time dependency in the reverse of the usual reaction in aqueous solvents. Enzymatic cholesterol linoleate synthesis follo...

journal_title：Biotechnology and applied biochemistry

authors： Mori S,Nakata Y,Endo H

更新日期：1992-06-01 00:00:00

abstract：:The plant polymer lignin is the greatest source of aromatic chemical structures on earth. Hence, the chemically diverse lignin monomers are valuable raw materials for fine chemicals, materials synthesis, and food and flavor industries. However, extensive use of this natural resource is hampered by the large number of ...

journal_title：Biotechnology and applied biochemistry

authors： Jansen F,Gillessen B,Mueller F,Commandeur U,Fischer R,Kreuzaler F

更新日期：2014-11-01 00:00:00