Anthrax toxin-mediated delivery of cholera toxin-A subunit into the cytosol of mammalian cells.

abstract：:The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently, we reported that a de novo carbene transferase, a tetra-α-helical c-type heme-containing protein, C45, is proficient at N-H insertion reactions, proceeding via the intermolecular transfer of a metallocarben...

journal_title：Biotechnology and applied biochemistry

authors： Stenner R,Anderson JLR

更新日期：2020-07-01 00:00:00

abstract：:Biologically active membrane proteins are difficult to isolate. Very often the isolated membrane proteins have low binding affinity or no biological integrity at all. Despite some success in isolation, one has to overcome the hurdles of obtaining sufficient quantity of the proteins and maintaining biological activity ...

journal_title：Biotechnology and applied biochemistry

authors： Wong RB,Lee AH,Rangan VS,Cheng KC

更新日期：2009-05-01 00:00:00

abstract：:The L-arginine uptake of resting platelets from type-2 diabetic patients and control subjects was measured and the kinetic parameters defined. The effect of platelet stimulation with agonists was also investigated. Kinetic studies showed that the K(m) value for L-arginine transport was not different in patients compar...

journal_title：Biotechnology and applied biochemistry

authors： Signorello MG,Giovine M,Pascale R,Cataldi L,Bordone C,Benatti U,Leoncini G

更新日期：2001-08-01 00:00:00

abstract：:Cordyceps sinensis (Chinese caterpillar fungus) is a fungus parasitic on the larvae of Lepidoptera and has been considered to be a precious tonic food and herbal medicine since ancient times in China. Recently, some fungal strains have been isolated from the fruiting bodies of wild C. sinensis, and some of them have b...

journal_title：Biotechnology and applied biochemistry

authors： Zhang W,Yang J,Chen J,Hou Y,Han X

更新日期：2005-08-01 00:00:00

abstract：:Fetuin is a plasma glycoprotein widely distributed in mammals. It has been used as a model protein for structural analyses and investigations into the biological properties of glycoproteins. A convenient one-step procedure for biospecific isolation of fetuin from fetal bovine serum was developed on the basis of wheatg...

journal_title：Biotechnology and applied biochemistry

authors： Cartellieri S,Hamer O,Helmholz H,Niemeyer B

更新日期：2002-04-01 00:00:00

abstract：:An extremely thermostable UDP-GlcNAc pyrophosphorylase has been purified from Thermus caldophilus GK24 by chromatographic methods including ion-exchange, hydrophobic interaction, and affinity chromatographies. The specific activity of the enzyme was enriched 41.8-fold, with a recovery of 2%. The molecular mass of the ...

journal_title：Biotechnology and applied biochemistry

authors： Kim JS,Koh S,Shin HJ,Lee DS,Lee SY

更新日期：1999-02-01 00:00:00

abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...

journal_title：Biotechnology and applied biochemistry

authors： Qiu C,Wang X,Zhang X,Li Z,Zhou Y,Kang J

更新日期：2020-08-15 00:00:00

abstract：:Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H2 , organic acids, ethanol, and cellulase has been explo...

journal_title：Biotechnology and applied biochemistry

authors： Kumar P,Maharjan A,Jun HB,Kim BS

更新日期：2019-03-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs), the largest family of cell-surface molecules involved in a number of biological and pathological processes, have recently emerged as key players in carcinogenesis and cancer progression. Orphan G protein-coupled receptors (oGPCRs) are a group of proteins lacking endogenous ligands....

journal_title：Biotechnology and applied biochemistry

authors： Cui X,Liu Y,Wang B,Xian G,Liu X,Tian X,Qin C

更新日期：2015-11-01 00:00:00

abstract：:Most insulin-dependent diabetes mellitus patients gen-erate conformational autoantibodies to the islet-cell 65-kDa variant of human glutamate decarboxylase (GAD65), and several immunochemical tests for the early detection of type-1 diabetes rely on GAD65 antibody (GADA) assessment using properly folded recombinant GAD...

journal_title：Biotechnology and applied biochemistry

authors： Santos J,Antón EA,Buslje CM,Valdez SN,Villanueva AL,Sica M,Iacono R,Maffia P,Poskus E,Ermácora MR

更新日期：2000-06-01 00:00:00

abstract：:St. John's wort ( Hypericum perforatum L.) is a herbaceous perennial distributed throughout the World that has been widely used in traditional medicine. H. perforatum produces several types of biologically active compound, including the hypericins--a family of light-activated anthraquinones, localized within specializ...

journal_title：Biotechnology and applied biochemistry

authors： Kirakosyan A,Sirvent TM,Gibson DM,Kaufman PB

更新日期：2004-02-01 00:00:00

abstract：:Expression of the VP2 gene of autonomous parvoviruses in insect cells with the use of the baculovirus system has led to the production of virus-like particles (VLPs) formed by the self-assembly of VP2. These VLPs are expressed at high levels and can easily be purified by salt fractionation. They are highly immunogenic...

journal_title：Biotechnology and applied biochemistry

authors： Casal JI

更新日期：1999-04-01 00:00:00

abstract：:Cinnamyl acetate was synthesized using immobilized lipase through transesterification between ethyl acetate and cinnamyl alcohol. In the solvent-free system, ethyl acetate acted as not only the acyl donor but also as the solvent of cinnamyl alcohol. Conversion (90.06%) was achieved after 3 H when transesterification w...

journal_title：Biotechnology and applied biochemistry

authors： Geng B,Wang M,Qi W,Su R,He Z

更新日期：2012-07-01 00:00:00

abstract：:The conformation of a protein refers to the three-dimensional arrangement of its constituent atoms. Since expression of the biological activity of a protein depends on its conformation, it is clear that full characterization of a protein involves an understanding of its three-dimensional structure. This review outline...

journal_title：Biotechnology and applied biochemistry

authors： Price NC

更新日期：2000-02-01 00:00:00

abstract：:The arrival of novel health crisis by a novel member of coronavirus group named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by World Health Organization took the whole world in global emergency by affecting 206 countries. The virus infects 206 countries with 86,839,226 confirmed cases, 61,565,949 r...

journal_title：Biotechnology and applied biochemistry

authors： Shivangi S,Meena LS

更新日期：2021-01-12 00:00:00

abstract：:Human urokinase was immobilized on an ethylene vinyl acetate copolymer surface. Soluble urokinase showed its maximum activity at pH 8.5, while the immobilized enzyme was most active at pH 9.0. Apparently, the shift in optimal pH was due to the polyanionic nature of the carrier surface on which the enzyme was immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Yabushita Y

更新日期：1988-06-01 00:00:00

abstract：:The main metabolic properties of human red blood cells (RBC) overloaded with glucose catabolizing enzymes such as hexokinase and glucose oxidase were evaluated. Human erythrocytes loaded with human hexokinase metabolized 3.1 +/- 0.2 mumol/h/ml RBC of glucose, an amount double that consumed by normal and unloaded cells...

journal_title：Biotechnology and applied biochemistry

authors： Rossi L,Bianchi M,Magnani M

更新日期：1992-04-01 00:00:00

abstract：:Succinylcholine apnea happens in cases of null butyrylcholinesterase activity after administration of preintubation succinylcholine. So far, there is no such popular test that can rapidly screen null butyrylcholinesterase activity from plasma. Development of a novel method for rapid screening of null butyrylcholineste...

journal_title：Biotechnology and applied biochemistry

authors： Nagpal N,Chowdhary S,Bhattacharyya R,Banerjee D

更新日期：2015-03-01 00:00:00

abstract：:The number of approaches to recombinant protein production in plants is greater than ever before. Development of these new and improved technologies as production platforms for plant-made pharmaceuticals has and will continue to create new commercial opportunities in the pharmaceutical sector. However, it is inevitabl...

journal_title：Biotechnology and applied biochemistry

authors： Paul M,Ma JK

更新日期：2011-01-01 00:00:00

abstract：:Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our p...

journal_title：Biotechnology and applied biochemistry

authors： Chen Z,Zhuo W,Wang Y,Ao X,An J

更新日期：2008-06-01 00:00:00

abstract：:Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (E...

journal_title：Biotechnology and applied biochemistry

authors： Lau MF,Chua KH,Sabaratnam V,Kuppusamy UR

更新日期：2020-08-28 00:00:00

abstract：:Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin-streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplification is overamplific...

journal_title：Biotechnology and applied biochemistry

authors： Shao K,Shi X,Zhu X,Cui L,Shao Q,Ma D

更新日期：2017-03-01 00:00:00

abstract：:High yields of extracellular alpha-galactosidase from fungal cultures were obtained by inducing enzyme production with guar gum (a galactomannan obtained from the seeds of Cyamopsis tetragonobola) as the sole carbon source. An alpha-galactosidase was isolated from the culture medium of Penicillium ochrochloron culture...

journal_title：Biotechnology and applied biochemistry

authors： Dey PM,Patel S,Brownleader MD

更新日期：1993-06-01 00:00:00

abstract：:The aim of this paper is to make the point on the fortieth years study on the β-glycosidase from Sulfolobus solfataricus. This enzyme represents one of the thermophilic biocatalysts, which is more extensively studied as witnessed by the numerous literature reports available since 1980. Comprehensive biochemical studie...

journal_title：Biotechnology and applied biochemistry

authors： Febbraio F,Ionata E,Marcolongo L

更新日期：2020-07-01 00:00:00

abstract：:This paper is concerned with the optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Auricularia polytricha by one-factor-at-a-time and uniform design (UD) methods. First, the one-factor-at-a-time method was adopted to investigate the effects of environmental factors (i.e....

journal_title：Biotechnology and applied biochemistry

authors： Xu CP,Yun JW

更新日期：2003-10-01 00:00:00

abstract：:Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrog...

journal_title：Biotechnology and applied biochemistry

authors： Chirila TV,Suzuki S,Papolla C

更新日期：2017-11-01 00:00:00

abstract：:Rapid progress has been made in the design and synthesis of oligomers and polymers that emulate the properties of natural proteins. Molecular bioengineering offers the chance to design and produce artificial polymeric proteins with tailored polymeric properties. The elastin-like polypeptides are a well-defined family ...

journal_title：Biotechnology and applied biochemistry

authors： Bandiera A,Taglienti A,Micali F,Pani B,Tamaro M,Crescenzi V,Manzini G

更新日期：2005-12-01 00:00:00

abstract：:Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for bo...

journal_title：Biotechnology and applied biochemistry

authors： Parascandola P,de Alteriis E

更新日期：1996-02-01 00:00:00

abstract：:The interpretation of the circular dichroism (c.d.) spectra of proteins to date requires additional secondary structural information of the proteins to be analysed (e.g., X-ray or n.m.r. data). Therefore these methods are inappropriate for a c.d. database whose secondary structures are unknown, as in the case of the m...

journal_title：Biotechnology and applied biochemistry

authors： Fasman GD

更新日期：1993-10-01 00:00:00

abstract：:It is now a well-known fact that alpha-CD (alpha-cyclodextrin) and Ca(2+) are two important folding agents in the re-activation and refolding processes of many denatured enzyme solutions. Bacillus sp. alpha-amylase was extensively denatured in a 6 M guanidinium chloride solution overnight. Under suitable renaturation ...

journal_title：Biotechnology and applied biochemistry

authors： Yazdanparast R,Khodarahmi R

更新日期：2005-04-01 00:00:00