One-step affinity purification of fetuin from fetal bovine serum.

abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...

journal_title：Biotechnology and applied biochemistry

authors： Hiragami F,Akiyama J,Koike Y,Kano Y

更新日期：2007-05-01 00:00:00

abstract：:An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferul...

journal_title：Biotechnology and applied biochemistry

authors： Kroon PA,Faulds CB,Williamson G

更新日期：1996-06-01 00:00:00

abstract：:Sometimes it is necessary to obtain thrombin from limited amounts of human plasma for laboratory assay. None of the available purification methods easily deals with this subject. The procedure described in the present paper uses a readily available pharmaceutical agent, argatroban, to construct an affinity matrix. Arg...

journal_title：Biotechnology and applied biochemistry

authors： Lefkowitz JB

更新日期：2005-10-01 00:00:00

abstract：:Fermentation of herb Polygonum hydropiper L. (PHL) and cassava pulp (CP) for feed additive production with simultaneous flavonoid dissolution was investigated, and a two-stage response surface methodology (RSM) based on Plackett-Burman factorial design (PB design) was used to optimize the flavonoid dissolution and pro...

journal_title：Biotechnology and applied biochemistry

authors： Song ZT,Zhu MJ

更新日期：2017-03-01 00:00:00

abstract：:In the present study, the growth and key metabolic features of a gene-transfected Drosophila melanogaster (fruitfly) S2 (Schneider 2) cell population (S2AcRVGP cells), cultured in Sf900-II medium, have been evaluated to provide substantial support for the development of a bioprocess to produce RVGP (rabies-virus glyco...

journal_title：Biotechnology and applied biochemistry

authors： Swiech K,da Silva CS,Arantes MK,dos Santos AS,Astray RM,Pereira CA,Suazo CA

更新日期：2008-01-01 00:00:00

abstract：:Human urokinase was immobilized on an ethylene vinyl acetate copolymer surface. Soluble urokinase showed its maximum activity at pH 8.5, while the immobilized enzyme was most active at pH 9.0. Apparently, the shift in optimal pH was due to the polyanionic nature of the carrier surface on which the enzyme was immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Yabushita Y

更新日期：1988-06-01 00:00:00

abstract：:The structural underpinnings of O₂ activation in P450s have been limited by the inability to trap unstable oxy complexes in the crystalline state for structure determination. To date there are only two known oxy P450 structures. Even so, much is known about O₂ activation in P450cam and the role that redox partner bind...

journal_title：Biotechnology and applied biochemistry

authors： Poulos TL,Madrona Y

更新日期：2013-01-01 00:00:00

abstract：:The interpretation of the circular dichroism (c.d.) spectra of proteins to date requires additional secondary structural information of the proteins to be analysed (e.g., X-ray or n.m.r. data). Therefore these methods are inappropriate for a c.d. database whose secondary structures are unknown, as in the case of the m...

journal_title：Biotechnology and applied biochemistry

authors： Fasman GD

更新日期：1993-10-01 00:00:00

abstract：:The effects of chitin [(1-->4)-2-acetamido-2-deoxy-beta-D-glucan] and its partially deacetylated derivatives, chitosans, on the human dermal fibroblast-mediated contraction of collagen lattices were examined in vitro as a model for the contraction of cutaneous wounds in vivo. Chitosan CL313A, a short-chain-length 89% ...

journal_title：Biotechnology and applied biochemistry

authors： Howling GI,Dettmar PW,Goddard PA,Hampson FC,Dornish M,Wood EJ

更新日期：2002-12-01 00:00:00

abstract：:Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...

journal_title：Biotechnology and applied biochemistry

authors： Li Q,Ng WT,Puah SM,Bhaskar RV,Soh LS,MacBeath C,Parakattil P,Green P,Wu JC

更新日期：2014-07-01 00:00:00

abstract：:Restoration of insulin secretion by transplantation of isolated islets is a treatment for type Ι diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate e...

journal_title：Biotechnology and applied biochemistry

authors： Li N,Zhang Y,Xiu Z,Wang Y,Chen L,Wang S,Li S,Guo X,Ma X

更新日期：2015-07-01 00:00:00

abstract：:Carbamate kinase has been prepared from Lactobacillus buchneri NCDO110. An approximately 91-fold increase in the specific activity of the enzyme was achieved. The purified extract exhibited a single band following polyacrylamide gel electrophoresis. The apparent molecular weight as determined by gel electrophoresis wa...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Nadra Chaud CA,Pesce de Ruiz Holgado A,Oliver G

更新日期：1986-02-01 00:00:00

abstract：:Acid phosphatase (EC 3.1.3.2) from Vigna aconitifolia seeds was purified to apparent homogeneity by using ammonium sulfate fractionation and cation-exchange chromatography [carboxymethyl (CM) cellulose]. The enzyme was 228-fold purified with 14.6% recovery. Analytical gel filtration chromatography on Sephadex G-200 co...

journal_title：Biotechnology and applied biochemistry

authors： Anand A,Srivastava PK

更新日期：2014-03-01 00:00:00

abstract：:Melamine adulteration of food is a public health concern. It has been seen that melamine causes disease in many organs. Melamine-induced kidney disease is a well-recognized clinicopathological entity. Inflammation is thought to be important in melamine-induced pathology. Melamine is expected to bind with albumin becau...

journal_title：Biotechnology and applied biochemistry

authors： Rajpoot M,Bhattacharyya R,Banerjee D,Sharma A

更新日期：2017-07-01 00:00:00

abstract：:A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN-DI) that happened during the long-term storage process. It was found that FMN-DI did not simpl...

journal_title：Biotechnology and applied biochemistry

authors： Huston S,Collins J,Sun F,Zhang T,Vaden TD,Zhang YP,Fu J

更新日期：2018-05-01 00:00:00

abstract：:Rapid progress has been made in the design and synthesis of oligomers and polymers that emulate the properties of natural proteins. Molecular bioengineering offers the chance to design and produce artificial polymeric proteins with tailored polymeric properties. The elastin-like polypeptides are a well-defined family ...

journal_title：Biotechnology and applied biochemistry

authors： Bandiera A,Taglienti A,Micali F,Pani B,Tamaro M,Crescenzi V,Manzini G

更新日期：2005-12-01 00:00:00

abstract：:A purple-spore, rhizosphere-inhabiting nematophagous fungus, further identified as Paecilomyces lilacinus, was found to grow on chitosanase-detecting plate. An induced endochitosanase having a molecular mass of 23 kDa was purified from the culture medium by a single cation-exchange column-chromatography step. Its opti...

journal_title：Biotechnology and applied biochemistry

authors： Chen YY,Cheng CY,Haung TL,Li YK

更新日期：2005-04-01 00:00:00

abstract：:The hydrolysis kinetics of native and denatured haemoglobin, using pepsin immobilized on aluminium oxide, was studied in order to produce hydrolysates containing bioactive peptides. Pepsin was immobilized on acidic alumina and on 2-ethanolamine- O -phosphate (2-EAOP)-modified acidic alumina. Surface charge of the two ...

journal_title：Biotechnology and applied biochemistry

authors： Ticu EL,Vercaigne-Marko D,Huma A,Artenie V,Toma O,Guillochon D

更新日期：2004-04-01 00:00:00

abstract：:alpha-Cyclodextrin (CD) was converted by Bacillus macerans cyclodextrin glucanotransferase into highly insoluble high-amylose starch with a low average degree of polymerization (56-73), in yields as high as 78% over a wide range of temperatures (25-70 degreesC). Ability to undergo this conversion was highly concentrat...

journal_title：Biotechnology and applied biochemistry

authors： Rendleman JA Jr,Knutson CA Jr

更新日期：1998-12-01 00:00:00

abstract：:Production of His-BbST [hexahistidine-tagged BbST (bubaline somatotropin)] by auto-induction in high-density shake-flask cultures coupled with a single-step, on-column purification and refolding strategy is described here. To optimize expression of BbST, different media and expression conditions were tested. The highe...

journal_title：Biotechnology and applied biochemistry

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2007-05-01 00:00:00

abstract：:Cordyceps sinensis (Chinese caterpillar fungus) is a fungus parasitic on the larvae of Lepidoptera and has been considered to be a precious tonic food and herbal medicine since ancient times in China. Recently, some fungal strains have been isolated from the fruiting bodies of wild C. sinensis, and some of them have b...

journal_title：Biotechnology and applied biochemistry

authors： Zhang W,Yang J,Chen J,Hou Y,Han X

更新日期：2005-08-01 00:00:00

abstract：:Cholesterol esterase modified with polyethylene glycol was able to dissolve in some highly hydrophobic solvents such as benzene and toluene, and catalyze the synthesis of cholesterol linoleate with time dependency in the reverse of the usual reaction in aqueous solvents. Enzymatic cholesterol linoleate synthesis follo...

journal_title：Biotechnology and applied biochemistry

authors： Mori S,Nakata Y,Endo H

更新日期：1992-06-01 00:00:00

abstract：:The introduction of different methodologies for the construction and screening of complex protein libraries has provided powerful means in protein engineering for the development of molecules with desired traits. A challenge faced in many situations is to adapt a given methodology for efficient and rapid identificatio...

journal_title：Biotechnology and applied biochemistry

authors： Löfdahl PA,Nygren PA

更新日期：2010-03-05 00:00:00

abstract：:Glutaraldehyde crosslinked, immunoprecipitated gulonolactone oxidase, injected intraperitoneally, has significant catalytic activity and is capable of providing long-term therapeutic benefit for the enzyme deficiency disease scurvy. The enzyme is tolerated even in repetitive doses. In the present study, however, we ha...

journal_title：Biotechnology and applied biochemistry

authors： Sato P,Lindemann D

更新日期：1986-04-01 00:00:00

abstract：:Arylacetonitrile-hydrolyzing nitrilase (E.C. 3.5.5.5) of Alcaligenes sp. MTCC 10675 has been purified by up to 46-fold to homogeneity and 32% yield using ammonium sulfate fractionation, Sephacryl S-300 gel permeation, and anion exchange chromatography. The molecular weight of the native enzyme was estimated to be 520 ...

journal_title：Biotechnology and applied biochemistry

authors： Bhatia SK,Mehta PK,Bhatia RK,Bhalla TC

更新日期：2014-07-01 00:00:00

abstract：:The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatog...

journal_title：Biotechnology and applied biochemistry

authors： Rigi G,Ghaedmohammadi S,Ahmadian G

更新日期：2019-05-01 00:00:00

abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...

journal_title：Biotechnology and applied biochemistry

authors： Banks JG,Board RG,Sparks NH

更新日期：1986-04-01 00:00:00

abstract：:The antifungal activity of iturin A and its interaction with erythrocyte membranes were enhanced in the presence of surfactin. The modification of the properties of iturin A was explained by the formation of mixed iturin A-surfactin micelles. Such mixed micelles were easily generated when both lipopeptides were in aqu...

journal_title：Biotechnology and applied biochemistry

authors： Thimon L,Peypoux F,Maget-Dana R,Roux B,Michel G

更新日期：1992-10-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of garlic (Allium sativum L.) on the diabetic nephropathy and oxidative stress induced by STZ (streptozotocin) in rats. Diabetes was induced in Male Sprague-Dawley rats by administering a single intraperitoneal injection of STZ (60 mg/kg of body weight). ...

journal_title：Biotechnology and applied biochemistry

authors： Mariee AD,Abd-Allah GM,El-Yamany MF

更新日期：2009-03-01 00:00:00

abstract：:In this study, the whole proteins from a Chinese three-striped box turtle (Cuora trifasciata) were extracted and hydrolyzed using three proteases (alcalase, papain, and protamex). By orthogonal experiments, the optimal hydrolysis conditions for producing peptides with the highest cancer cells growth inhibition activit...

journal_title：Biotechnology and applied biochemistry

authors： Mao X,He S,Zhang T,Guo X,Ge Y,Ma C,Zhang X

更新日期：2017-11-01 00:00:00