Argatroban-coupled Affi-Gel matrix for the purification of thrombin from plasma.

abstract：:A simple, specific, sensitive and reproducible ELISA has been developed to quantify the level of CPS (capsular polysaccharide) production in supernatants of Streptococcus pneumoniae cell cultures. CPSs from Strep. pneumoniae have been widely used as vaccine antigens. The quantification method is based on two type-23F ...

journal_title：Biotechnology and applied biochemistry

authors： Cruz-Leal Y,Menéndez T,Coizeau E,Espinosa RR,Canaan L,Blanco F,Carmenate T,Chang J,Quiñones D,Tamargo I,Cremata J,Verez-Bencomo V,Guillén G

更新日期：2006-05-01 00:00:00

abstract：:Carbamate kinase has been prepared from Lactobacillus buchneri NCDO110. An approximately 91-fold increase in the specific activity of the enzyme was achieved. The purified extract exhibited a single band following polyacrylamide gel electrophoresis. The apparent molecular weight as determined by gel electrophoresis wa...

journal_title：Biotechnology and applied biochemistry

authors： Manca de Nadra MC,Nadra Chaud CA,Pesce de Ruiz Holgado A,Oliver G

更新日期：1986-02-01 00:00:00

abstract：:Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an au...

journal_title：Biotechnology and applied biochemistry

authors： Jantra J,Zór K,Sanders M,De Saeger S,Hedström M,Mattiasson B

更新日期：2020-05-01 00:00:00

abstract：:Arylacetonitrile-hydrolyzing nitrilase (E.C. 3.5.5.5) of Alcaligenes sp. MTCC 10675 has been purified by up to 46-fold to homogeneity and 32% yield using ammonium sulfate fractionation, Sephacryl S-300 gel permeation, and anion exchange chromatography. The molecular weight of the native enzyme was estimated to be 520 ...

journal_title：Biotechnology and applied biochemistry

authors： Bhatia SK,Mehta PK,Bhatia RK,Bhalla TC

更新日期：2014-07-01 00:00:00

abstract：:This study was designed to investigate the protective effects of Acanthus ilicifolius alkaloid A [4-hydroxy-2-benzoxazolone (HBOA)] and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride (CCl4 )-induced liver fibrosis in rats. Spr...

journal_title：Biotechnology and applied biochemistry

authors： Wai KK,Liang Y,Zhou L,Cai L,Liang C,Liu L,Lin X,Wu H,Lin J

更新日期：2015-07-01 00:00:00

abstract：:In order to impart an ability for receptor-independent transmembrane transfer to water-soluble proteins, it has been suggested that they be hydrophobized by lipid groups (fatty acids, etc.). To this end, systems of reversed micelles of surfactants in organic solvents were used as reaction media for protein modificatio...

journal_title：Biotechnology and applied biochemistry

authors： Alakhov VYu,Kabanov AV,Batrakova EV,Koromyslova IA,Levashov AV,Severin ES

更新日期：1990-02-01 00:00:00

abstract：:Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...

journal_title：Biotechnology and applied biochemistry

authors： Li Q,Ng WT,Puah SM,Bhaskar RV,Soh LS,MacBeath C,Parakattil P,Green P,Wu JC

更新日期：2014-07-01 00:00:00

abstract：:Nicotine is a potent parasympathomimetic stimulant and an important natural alkaloid mainly found in the Nicotiana genus of plants. It can directly threaten ecological security and human health in tobacco waste, wastewater, and other forms of tobacco production. Therefore, it is the basis of nicotine pollution prevent...

journal_title：Biotechnology and applied biochemistry

authors： Ruan A,Gao Y,Fang C,Xu Y

更新日期：2018-11-01 00:00:00

abstract：:The hydrolysis kinetics of native and denatured haemoglobin, using pepsin immobilized on aluminium oxide, was studied in order to produce hydrolysates containing bioactive peptides. Pepsin was immobilized on acidic alumina and on 2-ethanolamine- O -phosphate (2-EAOP)-modified acidic alumina. Surface charge of the two ...

journal_title：Biotechnology and applied biochemistry

authors： Ticu EL,Vercaigne-Marko D,Huma A,Artenie V,Toma O,Guillochon D

更新日期：2004-04-01 00:00:00

abstract：:The efficient enzyme-catalysed reaction of morpholines as model structures for bioactive compounds with para-dihydroxylated aromatic systems was carried out using the oxidoreductase laccase and atmospheric oxygen to produce eight novel morpholine-substituted aromatics. The laccase of Myceliophthora thermophila was use...

journal_title：Biotechnology and applied biochemistry

authors： Hahn V,Mikolasch A,Wende K,Bartrow H,Lindequist U,Schauer F

更新日期：2009-11-13 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is a hematologic malignancy characterized by excessive growth of myeloid cells and their progenitors. The proportion of spectrin dimers compared to tetramers extracted from membranes at 4 degrees C, under low ionic strength conditions, increased in CML erythrocytes. These also displa...

journal_title：Biotechnology and applied biochemistry

authors： Basu J,Kundu M,Rakshit MM,Chakrabarti P

更新日期：1990-10-01 00:00:00

abstract：:A purple-spore, rhizosphere-inhabiting nematophagous fungus, further identified as Paecilomyces lilacinus, was found to grow on chitosanase-detecting plate. An induced endochitosanase having a molecular mass of 23 kDa was purified from the culture medium by a single cation-exchange column-chromatography step. Its opti...

journal_title：Biotechnology and applied biochemistry

authors： Chen YY,Cheng CY,Haung TL,Li YK

更新日期：2005-04-01 00:00:00

abstract：:St. John's wort ( Hypericum perforatum L.) is a herbaceous perennial distributed throughout the World that has been widely used in traditional medicine. H. perforatum produces several types of biologically active compound, including the hypericins--a family of light-activated anthraquinones, localized within specializ...

journal_title：Biotechnology and applied biochemistry

authors： Kirakosyan A,Sirvent TM,Gibson DM,Kaufman PB

更新日期：2004-02-01 00:00:00

abstract：:Among the heat-shock proteins (HSPs) induced in response to heat-shock (HS) in all organisms, the low-molecular-mass (LMM) HSPs are the most abundant and unique in plants. The HSP genes at the transcriptional and the translational levels have been well-characterized; however, relatively little is known about the physi...

journal_title：Biotechnology and applied biochemistry

authors： Yeh KW,Jinn TL,Yeh CH,Chen YM,Lin CY

更新日期：1994-02-01 00:00:00

abstract：:Mouse band-3 crosslinked carrier erythrocytes have been prepared. [125I]Carbonic anhydrase (CA) has been encapsulated into mouse erythrocytes. Then, loaded erythrocytes were labelled with 51Cr. Eventually, these doubly labelled cells were crosslinked with band-3 crosslinking reagents. [125I]CA was shown to have cytoso...

journal_title：Biotechnology and applied biochemistry

authors： Jordán JA,Alvarez FJ,Tejedor MC,Díez JC

更新日期：1999-02-01 00:00:00

abstract：:The immobilization of F(0)F(1)-ATPase in uniform orientation is reported. The biotinylated and histidine-tagged subunits of the bacterial F(0)F(1)-ATPase complex were used for immobilization of the complex on artificial semi-permeable membranes resulting in 88+/-7.8 and 72+/-5.2% coupling of the enzymes. The immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2004-06-01 00:00:00

abstract：:The human placenta is a rich raw material for production of many biopharmaceutical products. Here we describe a co-purification process for the production of four different proteins from haemolysed human placenta blood: IgG, catalase (Cat), superoxide dismutase (Sod) and albumin (Alb). The process can be divided in tw...

journal_title：Biotechnology and applied biochemistry

authors： Grellet S,Martins EA,Maimoni Gonçalves V,Yague Lopes AP,Raw I,Cabrera-Crespo J

更新日期：2001-12-01 00:00:00

abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...

journal_title：Biotechnology and applied biochemistry

authors： Banks JG,Board RG,Sparks NH

更新日期：1986-04-01 00:00:00

abstract：:[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of ...

journal_title：Biotechnology and applied biochemistry

authors： Abiko T,Shishido H,Sekino H

更新日期：1986-04-01 00:00:00

abstract：:Future developments in gene therapy and DNA vaccination depend on cost-effective large-scale production of pharmaceutical-grade pDNA (plasmid DNA). Given the large amount of impurities present in the feedstock, purification processes that have high specificity and capacity at a moderate cost are required. In the prese...

journal_title：Biotechnology and applied biochemistry

authors： Frerix A,Müller M,Kula MR,Hubbuch J

更新日期：2005-08-01 00:00:00

abstract：:Production of His-BbST [hexahistidine-tagged BbST (bubaline somatotropin)] by auto-induction in high-density shake-flask cultures coupled with a single-step, on-column purification and refolding strategy is described here. To optimize expression of BbST, different media and expression conditions were tested. The highe...

journal_title：Biotechnology and applied biochemistry

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2007-05-01 00:00:00

abstract：:Fermentation of herb Polygonum hydropiper L. (PHL) and cassava pulp (CP) for feed additive production with simultaneous flavonoid dissolution was investigated, and a two-stage response surface methodology (RSM) based on Plackett-Burman factorial design (PB design) was used to optimize the flavonoid dissolution and pro...

journal_title：Biotechnology and applied biochemistry

authors： Song ZT,Zhu MJ

更新日期：2017-03-01 00:00:00

abstract：:Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for bo...

journal_title：Biotechnology and applied biochemistry

authors： Parascandola P,de Alteriis E

更新日期：1996-02-01 00:00:00

abstract：:The α-amylase from soya bean seeds was purified by affinity precipitation, resulting in approx. 20-fold purification with approx. 84% recovery. The purified α-amylase had an optimum pH of 5.5, optimum temperature of 75 °C, Arrhenius energy of activation of 6.03 kcal/mol (1 kcal≈4.184 kJ) and a Km of 2.427 mg/ml (starc...

journal_title：Biotechnology and applied biochemistry

authors： Prakash O,Jaiswal N

更新日期：2010-12-01 00:00:00

abstract：:A sensitive sandwich enzyme immunoassay (e.i.a.) for serrapeptase (TSP), an orally available anti-inflammatory proteinase, was established using affinity-purified anti-TSP rabbit IgG and its Fab' fragment conjugated with horseradish peroxidase as the first and the second antibodies respectively. TSP in the plasma was ...

journal_title：Biotechnology and applied biochemistry

authors： Moriya N,Nakata M,Nakamura M,Takaoka M,Iwasa S,Kato K,Kakinuma A

更新日期：1994-08-01 00:00:00

abstract：:Lipases are surface-active enzymes, acting on their substrates at the polar/nonpolar interface in emulsions. This study was aimed to test whether their activity, specificity, and the rates of formation/degradation of the various hydrolysis intermediates (i.e., mono- and diglycerides of interest as surface-active agent...

journal_title：Biotechnology and applied biochemistry

authors： Carpen A,Bonomi F,Iametti S,Marengo M

更新日期：2019-07-01 00:00:00

abstract：:The variation with pH of the kinetic parameters of penicillin acylase from Kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. The pH-dependence of log (V/Km) for penicillin G showed that a group with a pK value over 4.7 must be deprotonated and t...

journal_title：Biotechnology and applied biochemistry

authors： Martín J,Prieto I,Mancheño JM,Barbero JL,Arche R

更新日期：1993-06-01 00:00:00

abstract：:The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...

journal_title：Biotechnology and applied biochemistry

authors： Son HJ,Heo MS,Kim YG,Lee SJ

更新日期：2001-02-01 00:00:00

abstract：:Sephadex was derivatized consecutively with Staphylococcus Protein A (SpA) and cell-specific antibodies, and the binding of cells to the resulting material was examined. For comparison, cell binding to commercially obtained SpA-Sepharose was determined. Sephadex G-10, carboxylated by reaction with glycine and activate...

journal_title：Biotechnology and applied biochemistry

authors： Besselink G,de Korte D

更新日期：2002-02-01 00:00:00

abstract：:Synthetic membranes containing 10% acrylamide units were subjected to activation with formaldehyde at pH 7.5 and 45 degrees C. Trypsin, invertase, and urease were bound to this activated membrane and the kinetic properties of immobilized enzymes were studied. The permeability of the membrane for distilled water manife...

journal_title：Biotechnology and applied biochemistry

authors： Krysteva MA,Shopova BI,Yotova LY,Karasavova MI

更新日期：1991-02-01 00:00:00