Covalent binding of enzymes to synthetic membranes containing acrylamide units, using formaldehyde.

abstract：:Two monoclonal antibodies against human myeloperoxidase, designated 3-2H3 and 4-2C11, were produced and characterized. Both bound to the native enzyme, but neither bound to the denatured enzyme or to its two denatured subunits. 4-2C11 bound to the three types of leukocyte myeloperoxidase, I, II, and III, as well as to...

journal_title：Biotechnology and applied biochemistry

authors： Hur SJ,Yamada M

更新日期：1986-12-01 00:00:00

abstract：:In the present study, the effect of the neuroleptics chlorpromazine (2-chloro-N,N-dimethyl-10H-phenothiazine-10-propanamine) and trifluoperazine {10-[3-(4-methylpiperazin-l-yl)-propyl]-2-trifluoromethyl)-10-H- [phenothiazine}-10H-phenothiazine¿ on the EPR-spectroscopic properties of ferrous human adult nitrosylated ha...

journal_title：Biotechnology and applied biochemistry

authors： Ascenzi P,Bertollini A,Coletta M,Lucacchini A

更新日期：1999-10-01 00:00:00

abstract：:In the erythrocyte membrane, the mobility of band 3 protein, the receptor for concanavalin A (Con A), is drastically reduced by the membrane skeleton. Yet, the vesicles free of membrane skeletal proteins, isolated from the highly agglutinable proteinase-treated cells, are found to be devoid of Con A agglutinability. T...

journal_title：Biotechnology and applied biochemistry

authors： Pestonjamasp KN,Gokhale SM,Mehta NG

更新日期：1990-10-01 00:00:00

abstract：:Cholesterol esterase modified with polyethylene glycol was able to dissolve in some highly hydrophobic solvents such as benzene and toluene, and catalyze the synthesis of cholesterol linoleate with time dependency in the reverse of the usual reaction in aqueous solvents. Enzymatic cholesterol linoleate synthesis follo...

journal_title：Biotechnology and applied biochemistry

authors： Mori S,Nakata Y,Endo H

更新日期：1992-06-01 00:00:00

abstract：:The interaction of four lectins from crops of the legume family with Saccharomyces cerevisiae alpha-mannan, and also with two glycoenzymes containing mainly alpha-mannan moieties, has been studied. The interaction was characterized by a quantitative precipitation assay. The results of precipitation differ with respect...

journal_title：Biotechnology and applied biochemistry

authors： Mislovicová D,Gemeiner P,Sandula J,Masárová J,Vikartovská A,Docolomanský P

更新日期：2000-04-01 00:00:00

abstract：:An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferul...

journal_title：Biotechnology and applied biochemistry

authors： Kroon PA,Faulds CB,Williamson G

更新日期：1996-06-01 00:00:00

abstract：:Cell proliferation is a complex and important event in atherosclerosis, aging and cancer, and is under the control of signalling pathways. These signalling pathways in turn are effected by the presence of a number of chemicals. For this purpose, we have checked the effect of two chemicals on the proliferation of skin ...

journal_title：Biotechnology and applied biochemistry

authors： Onat D,Boscoboinik D,Azzi A,Basaga H

更新日期：1999-06-01 00:00:00

abstract：:The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was ...

journal_title：Biotechnology and applied biochemistry

authors： Somkuti GA,Solaiman DK,Johnson TL,Steinberg DH

更新日期：1991-04-01 00:00:00

abstract：:Single base mismatched oligonucleotides related to the rpoB gene of Mycobacterium tuberculosis, the mutations of which cause drug resistance of the infectious agent, were detected and discriminated using a surface plasmon resonance biosensor system. Thiol-modified oligonucleotides of the selected sequence (the probe) ...

journal_title：Biotechnology and applied biochemistry

authors： Rachkov A,Patskovsky S,Soldatkin A,Meunier M

更新日期：2013-07-01 00:00:00

abstract：:Talpha1 (thymosin alpha 1) is important in treating immunodeficiency and other diseases. In order to study the feasibility of expressing Talpha1 in plants, as the first attempt, we designed and synthesized the Talpha1 gene according to the plant codon usage preference and constructed the 4xTalpha1 concatemer (four cop...

journal_title：Biotechnology and applied biochemistry

authors： Chen Y,Zhao L,Shen G,Cui L,Ren W,Zhang H,Qian H,Tang K

更新日期：2008-01-01 00:00:00

abstract：:The number of approaches to recombinant protein production in plants is greater than ever before. Development of these new and improved technologies as production platforms for plant-made pharmaceuticals has and will continue to create new commercial opportunities in the pharmaceutical sector. However, it is inevitabl...

journal_title：Biotechnology and applied biochemistry

authors： Paul M,Ma JK

更新日期：2011-01-01 00:00:00

abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...

journal_title：Biotechnology and applied biochemistry

authors： Wen CM,Tseng CS,Cheng CY,Li YK

更新日期：2002-06-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is widely used for the production of recombinant proteins for a variety of applications; however, a number of challenges are typically encountered by researchers depending on the properties of the specific proteins in question. Here, we describe technical issues we have...

journal_title：Biotechnology and applied biochemistry

authors： Belak ZR,Nair M,Ovsenek N

更新日期：2011-05-01 00:00:00

abstract：:A new α-neoagarobiose hydrolase (NABH) called AgaWH117 was cloned from Agarivorans gilvus WH0801. The gene encoding this hydrolase consists of 1,086 bp and encodes a protein containing 361 amino acids. This new NABH showed 74% amino acid sequence identity with other known NABHs. The molecular mass of the recombinant A...

journal_title：Biotechnology and applied biochemistry

authors： Liu N,Yang M,Mao X,Mu B,Wei D

更新日期：2016-03-01 00:00:00

abstract：:The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc....

journal_title：Biotechnology and applied biochemistry

authors： Takegawa K,Tohda H,Sasaki M,Idiris A,Ohashi T,Mukaiyama H,Giga-Hama Y,Kumagai H

更新日期：2009-06-22 00:00:00

abstract：:Bacillus subtilis strain DM-03, which is isolated from starter culture used for the production of alcohol by local Assam tribes, grows optimally at 52-55 degrees C and secretes a significant amount of alpha-amylase at pH 8.0 into the culture media. This alpha-amylase, purified by ion-exchange, gel-filtration and rever...

journal_title：Biotechnology and applied biochemistry

authors： Das K,Doley R,Mukherjee AK

更新日期：2004-12-01 00:00:00

abstract：:The use of glutaraldehyde (GTA) for cross-linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross-linking agent for tissue implants, we have evaluated a number of cross-linking procedures shown previousl...

journal_title：Biotechnology and applied biochemistry

authors： Simmons DM,Kearney JN

更新日期：1993-02-01 00:00:00

abstract：:Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our p...

journal_title：Biotechnology and applied biochemistry

authors： Chen Z,Zhuo W,Wang Y,Ao X,An J

更新日期：2008-06-01 00:00:00

abstract：:Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in de...

journal_title：Biotechnology and applied biochemistry

authors： Chen L,Guo S,Wu L,Hao C,Xu W,Zhang J

更新日期：2015-05-01 00:00:00

abstract：:As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...

journal_title：Biotechnology and applied biochemistry

authors： Hasegawa M,Kaku T,Kuroda M,Ise N,Kitano H

更新日期：1992-02-01 00:00:00

abstract：:The dynamics of dissolved oxygen (DO) and shear stress in the vasculature microenvironment play a major role in determining the fate of stem cells in adults and during early embryonic development. In this study, we present a microbioreactor (MBR) that provides independent control over oxygen tension and shear stress i...

journal_title：Biotechnology and applied biochemistry

authors： Abaci HE,Devendra R,Soman R,Drazer G,Gerecht S

更新日期：2012-03-01 00:00:00

abstract：:The therapeutic potential of microRNA-21 (miR-21) small-molecule inhibitors has been of particular interest to medicinal chemists. Moreover, the development of more facile screening methods is lacking. In the present study, two potential screening strategies for miR-21 small-molecule inhibitor including the stem-loop ...

journal_title：Biotechnology and applied biochemistry

authors： Hei YY,Guo YX,Jiang CS,Wang S,Lu SM,Zhang SQ

更新日期：2019-09-01 00:00:00

abstract：:Tissue engineering has been conducted in the study of cardiovascular grafts for many years. Many obstacles have been overcome in this rapidly changing field, but one difficulty has remained until now: the large number of endothelial cells (ECs) needed for seeding the inner layer of bypass graft. Recent advances in end...

journal_title：Biotechnology and applied biochemistry

authors： Punshon G,Vara DS,Sales KM,Seifalian AM

更新日期：2011-09-01 00:00:00

abstract：:A truncated precursor form of prostate-specific antigen (PSA), [-2]proPSA, is a well-known biomarker for prostate cancer. To develop a biomarker assay, highly purified [-2]proPSA is required as a standard reference and for generation of a specific antibody. In this study, we generated an efficient mammalian expression...

journal_title：Biotechnology and applied biochemistry

authors： Hwang D,Yoon A,Kim S,Kim H,Chung J

更新日期：2017-05-01 00:00:00

abstract：:Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...

journal_title：Biotechnology and applied biochemistry

authors： Li Q,Ng WT,Puah SM,Bhaskar RV,Soh LS,MacBeath C,Parakattil P,Green P,Wu JC

更新日期：2014-07-01 00:00:00

abstract：:[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of ...

journal_title：Biotechnology and applied biochemistry

authors： Abiko T,Shishido H,Sekino H

更新日期：1986-04-01 00:00:00

abstract：:Marine biomass, especially the algal biomass, is currently considered to be one of the most potential candidates for biofuels conversion during the development of biomass utilization. In this study, a diluted sulfuric acid pretreatment method was established for biobutanol from red algal biomass Gelidium amansii using...

journal_title：Biotechnology and applied biochemistry

authors： Hong Y,Chen C,Wu YR

更新日期：2020-09-01 00:00:00

abstract：:hES (human embryonic stem) cells hold tremendous potential in the newly emerging field of regenerative medicine, in addition to being a useful tool in basic scientific research and for pharmacological and cytotoxicity screening. However, an essential prerequisite for the future widespread application of hES cells are ...

journal_title：Biotechnology and applied biochemistry

authors： Heng BC,Kuleshova LL,Bested SM,Liu H,Cao T

更新日期：2005-04-01 00:00:00

abstract：:Medulloblastoma is the most common malignant pediatric brain tumor in children. GPR137 is a ubiquitously expressed gene in the central nervous system. It has been reported that GPR137 modulates malignant proliferation of glioma cells. However, the relationship between GPR137 and medulloblastoma is still unknown. In th...

journal_title：Biotechnology and applied biochemistry

authors： Wang C,Liang Q,Chen G,Jing J,Wang S

更新日期：2015-11-01 00:00:00

abstract：:A novel iron superoxide dismutase (Fe-SOD) gene from Jatropha curcas was cloned and expressed in Escherichia coli BL21 (DE3). This recombinant enzyme was isolated by a combined procedure involving immobilized metal-ion affinity chromatography and ion-exchange chromatography. The apparent molecular mass of this purifie...

journal_title：Biotechnology and applied biochemistry

authors： Ou-Yang C,Cai F,Gao S,Niu B,Wang S,Chen F

更新日期：2012-09-01 00:00:00