Abstract:
:The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was confirmed with Southern blot analysis using a biotinylated probe. Thin-layer chromatographic analysis showed the expression of the Streptomyces cholesterol oxidase gene resulting in the oxidation of cholesterol to 4-cholesten-3-one. S. thermophilus may be a suitable host for the expression of other genes regulating prokaryotic cholesterol metabolism.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Somkuti GA,Solaiman DK,Johnson TL,Steinberg DHsubject
Has Abstractpub_date
1991-04-01 00:00:00pages
238-45issue
2eissn
0885-4513issn
1470-8744journal_volume
13pub_type
杂志文章abstract::The antifungal activity of iturin A and its interaction with erythrocyte membranes were enhanced in the presence of surfactin. The modification of the properties of iturin A was explained by the formation of mixed iturin A-surfactin micelles. Such mixed micelles were easily generated when both lipopeptides were in aqu...
journal_title:Biotechnology and applied biochemistry
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doi:
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:
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journal_title:Biotechnology and applied biochemistry
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doi:
更新日期:1996-02-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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