Abstract：
:In our work, one-step electro-deposition method was adopted to produce polyaniline (PANI) and functional multiwalled carbon nanotubes (f-MWCNTs) films on glass carbon electrodes, and the modified electrodes were applied as an electrochemical sensor for determination of 10-hydroxycamptothecine (10-HCPT). The f-MWCNTs were handled by ultrasound processing in concentrated oxidizing acid solution, which can obtain a wonderful dissolution in water and attach new functional groups, such as -COOH and -OH. Then, aniline monomer could polymerize on the surface easily. The surface characterization was investigated using various techniques including scanning electron microscope, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and X-ray diffraction, and electro-catalytic properties were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimal conditions, the resulting of PANI/f-MWCNTs sensor showed a wide linear range (3 × 10-9 to 7 × 10-7 mol L-1 ) and a low detection limit (1 × 10-9 mol L-1 ), which is attributing to its large special surface area and good conductivity. Moreover, the modified electrodes are convenient to fabricate, which can be used to detect 10-HCPT in urine samples successfully.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Meng L,Dai J,Rong S,Gao H,Zhang Z,Zhang Y,Chang D,Pan Hdoi
10.1002/bab.2041subject
Has Abstractpub_date
2020-09-24 00:00:00eissn
0885-4513issn
1470-8744pub_type
杂志文章abstract：:Glutaraldehyde crosslinked, immunoprecipitated gulonolactone oxidase, injected intraperitoneally, has significant catalytic activity and is capable of providing long-term therapeutic benefit for the enzyme deficiency disease scurvy. The enzyme is tolerated even in repetitive doses. In the present study, however, we ha...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:G-protein-coupled receptors (GPCRs), the largest family of cell-surface molecules involved in a number of biological and pathological processes, have recently emerged as key players in carcinogenesis and cancer progression. Orphan G protein-coupled receptors (oGPCRs) are a group of proteins lacking endogenous ligands....
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1326
更新日期：2015-11-01 00:00:00
abstract：:alpha-Cyclodextrin (CD) was converted by Bacillus macerans cyclodextrin glucanotransferase into highly insoluble high-amylose starch with a low average degree of polymerization (56-73), in yields as high as 78% over a wide range of temperatures (25-70 degreesC). Ability to undergo this conversion was highly concentrat...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with platinum nanoparticles (Pt) and blocking reagent horseradish peroxidase (HRP) as enhancers. A Nafion®-graphene-coated electrode was first modified with an electrochemical probe of methylene blue (MB) through elect...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.33
更新日期：2011-07-01 00:00:00
abstract：:We studied, for the first time, characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of enzyme conformational stability and structural behaviour induced by temperature as a function of pH using enzymic assays, differential scanning calorimetry, fluo...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20000064
更新日期：2000-12-01 00:00:00
abstract：:The present study elaborates a cost-effective and transfectant-free method for generating recombinant Bombyx mori (silkworm) nucleopolyhedrovirus in silkworm larvae and pupae by injecting invasive Escherichia coli carrying BmBacmid [BmNPV (B. mori nucleopolyhedrovirus)-Bacmid] into larval haemocoel. Up to 109 PFU (pla...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20100148
更新日期：2010-12-01 00:00:00
abstract：:A new system equipped with a computer-controlled multiple activity analyzer has been developed for the efficient purification of multiple enzymes. The system consists of the following units: conventional enzyme fractionation system with a peristaltic pump, liquid chromatographic column, fraction collector, and uv moni...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Chronic myelogenous leukemia (CML) is a hematologic malignancy characterized by excessive growth of myeloid cells and their progenitors. The proportion of spectrin dimers compared to tetramers extracted from membranes at 4 degrees C, under low ionic strength conditions, increased in CML erythrocytes. These also displa...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1990-10-01 00:00:00
abstract：:Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrog...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1552
更新日期：2017-11-01 00:00:00
abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20020001
更新日期：2002-06-01 00:00:00
abstract：:High yields of extracellular alpha-galactosidase from fungal cultures were obtained by inducing enzyme production with guar gum (a galactomannan obtained from the seeds of Cyamopsis tetragonobola) as the sole carbon source. An alpha-galactosidase was isolated from the culture medium of Penicillium ochrochloron culture...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:A pathway for the synthesis of the repeating tetrasaccharide units in gellan gum from Pseudomonas elodea is proposed. The enzymes presumed to be involved in the synthesis of the activated precursors UDP-glucose, TDP-rhamnose, and UDP-glucuronic acid were detected and assayed in crude cell extracts of the gellan-produc...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:In the present study, the effect of the neuroleptics chlorpromazine (2-chloro-N,N-dimethyl-10H-phenothiazine-10-propanamine) and trifluoperazine {10-[3-(4-methylpiperazin-l-yl)-propyl]-2-trifluoromethyl)-10-H- [phenothiazine}-10H-phenothiazine¿ on the EPR-spectroscopic properties of ferrous human adult nitrosylated ha...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:A truncated precursor form of prostate-specific antigen (PSA), [-2]proPSA, is a well-known biomarker for prostate cancer. To develop a biomarker assay, highly purified [-2]proPSA is required as a standard reference and for generation of a specific antibody. In this study, we generated an efficient mammalian expression...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1481
更新日期：2017-05-01 00:00:00
abstract：:The use of glutaraldehyde (GTA) for cross-linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross-linking agent for tissue implants, we have evaluated a number of cross-linking procedures shown previousl...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:Pancreatic duodenum homeobox protein-1 (PDX1) is a master regulatory gene in pancreatic development. Reprogramming of mesenchymal stem cells (MSCs) is a promising tool for producing insulin-producing cells. In this study, lentivirus harboring PDX1 (LV-PDX1) has been used for persistence gene expression in MSCs. The ob...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1013
更新日期：2012-05-01 00:00:00
abstract：:The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc....
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1042/BA20090048
更新日期：2009-06-22 00:00:00
abstract：:Yeast cytochrome c peroxidase (CcP) was purified from baker's yeast and immobilised onto a nylon membrane. The kinetics of the soluble and immobilised forms of the enzyme were investigated for the catalysed oxidation of potassium ferrocyanide in the presence of H2O2 and m-chloroperoxybenzoic acid. The pH dependence of...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:The use of the recombinant BoNT/A-Hc (carboxylic domain of the Clostridium botulinum neurotoxin heavy chain) has been proposed as a vaccine candidate for botulism. This fragment contains the principle protective antigenic determinant. In the present study, in order to maximize recombinant protein expression, after ver...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20090315
更新日期：2010-05-24 00:00:00
abstract：:Restoration of insulin secretion by transplantation of isolated islets is a treatment for type Ι diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate e...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1295
更新日期：2015-07-01 00:00:00
abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:The phosphatase and tensin homolog (PTEN) gene plays a crucial role in signal transduction by negatively regulating the PI3K signaling pathway. It is the most frequent mutated gene in many human-related cancers. Considering its critical role, a functional analysis of missense mutations of PTEN gene was undertaken in t...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1483
更新日期：2017-09-01 00:00:00
abstract：:Cordyceps sinensis (Chinese caterpillar fungus) is a fungus parasitic on the larvae of Lepidoptera and has been considered to be a precious tonic food and herbal medicine since ancient times in China. Recently, some fungal strains have been isolated from the fruiting bodies of wild C. sinensis, and some of them have b...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20040183
更新日期：2005-08-01 00:00:00
abstract：:Single base mismatched oligonucleotides related to the rpoB gene of Mycobacterium tuberculosis, the mutations of which cause drug resistance of the infectious agent, were detected and discriminated using a surface plasmon resonance biosensor system. Thiol-modified oligonucleotides of the selected sequence (the probe) ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1101
更新日期：2013-07-01 00:00:00
abstract：:A new α-neoagarobiose hydrolase (NABH) called AgaWH117 was cloned from Agarivorans gilvus WH0801. The gene encoding this hydrolase consists of 1,086 bp and encodes a protein containing 361 amino acids. This new NABH showed 74% amino acid sequence identity with other known NABHs. The molecular mass of the recombinant A...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1363
更新日期：2016-03-01 00:00:00
abstract：:So far, many studies have focused on biodegradable scaffolds for tissue engineering purposes. Herein, a starch-based biodegradable scaffold was fabricated by the freeze-drying method and cross-linked using a different concentration of 3-glycidoxypropyl-trimethoxysilane (GPTMS). Field emission scanning electron microsc...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1694
更新日期：2019-01-01 00:00:00
abstract：:We have analysed the status and transcriptional activity of the bovine papillomavirus-I (complete BPV-I genome)-based expression vector pCES in CI27i-cell-line-derived 3TI cells used for the industrial production of recombinant human erythropoietin (rhuEpo). Complete tandem head-to-tail integration of about 600 vector...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:The dynamics of dissolved oxygen (DO) and shear stress in the vasculature microenvironment play a major role in determining the fate of stem cells in adults and during early embryonic development. In this study, we present a microbioreactor (MBR) that provides independent control over oxygen tension and shear stress i...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1010
更新日期：2012-03-01 00:00:00
abstract：:Glutamate dehydrogenase (GDH) and alcohol dehydrogenase (ADH) have been encapsulated in sheep and human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At a fixed enzyme level in the dialysis bag (100 units/ml of RBCs), a significant encapsulation yield was observed for ADH, both in human ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.2006
更新日期：2020-08-15 00:00:00
