Abstract:
:Yeast cytochrome c peroxidase (CcP) was purified from baker's yeast and immobilised onto a nylon membrane. The kinetics of the soluble and immobilised forms of the enzyme were investigated for the catalysed oxidation of potassium ferrocyanide in the presence of H2O2 and m-chloroperoxybenzoic acid. The pH dependence of the two forms of the enzyme differed. Although both the soluble and the immobilised enzymes showed optimal activity at pH 6.2, a different kinetic behaviour was demonstrated. Both forms of the enzyme showed similar activity toward H2O2, although when m-chloroperoxybenzoic acid was replaced as the electron acceptor, the immobilised form of the enzyme had a reduced turnover number and an increased Km. The activation energy of immobilised CcP was greater in the presence of both H2O2 [16.6 kJ mol-1] and m-chloroperoxybenzoic acid [37.9 kJ mol-1] than for soluble CcP [11.4 and 23.4 kJ mol-1, respectively]. The activities of both soluble and immobilised CcP were greatly reduced above 45 degrees C, although at higher temperatures the immobilised enzyme retained a relatively greater percentage of its maximum activity.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Cooper JM,McNeil CJ,Bannister JVsubject
Has Abstractpub_date
1991-08-01 00:00:00pages
93-103issue
1eissn
0885-4513issn
1470-8744journal_volume
14pub_type
杂志文章abstract::Glycoprotein D (gD2) is the most important candidate antigen for herpes simplex virus type 2 (HSV-2) vaccine development. Establishment of a stable eukaryotic cell line to overexpress gD2 and an efficient purification process to purify is essential for the development of subunit vaccine against HSV-2. The DNA sequence...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1386
更新日期:2016-05-01 00:00:00
abstract::Biological mineralization is a highly programmed process in which inorganic minerals reassociate under the strict control of the extracellular matrix to form minerals with special functions and patterns. Shells are biominerals, and their synthesis is finely regulated by organic matrix including matrix proteins, polysa...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1752
更新日期:2019-07-01 00:00:00
abstract::HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/ba20020027
更新日期:2002-12-01 00:00:00
abstract::Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new ti...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:
更新日期:1990-10-01 00:00:00
abstract::The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::Bovine serum albumin (BSA) microspheres with an average diameter of 12.5 micron were prepared by crosslinking of BSA molecules with glutaraldehyde in the presence of polymethylmethacrylate dissolved in chloroform-toluene. Trypsin and anti-human IgG antibody were immobilized onto their surfaces by the glutaraldehyde-ac...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::The human placenta is a rich raw material for production of many biopharmaceutical products. Here we describe a co-purification process for the production of four different proteins from haemolysed human placenta blood: IgG, catalase (Cat), superoxide dismutase (Sod) and albumin (Alb). The process can be divided in tw...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/ba20010023
更新日期:2001-12-01 00:00:00
abstract::Human erythrocytes from ten patients with chronic obstructive pulmonary disease (COPD) were loaded with increasing amounts of dexamethasone 21-phosphate and were re-infused into the original donors. Drug-loaded erythrocytes acted as circulating bioreactors, converting the non-diffusible dexamethasone 21-phosphate into...
journal_title:Biotechnology and applied biochemistry
pub_type: 临床试验,杂志文章
doi:10.1042/ba20000087
更新日期:2001-04-01 00:00:00
abstract::Acid phosphatase (EC 3.1.3.2) from Vigna aconitifolia seeds was purified to apparent homogeneity by using ammonium sulfate fractionation and cation-exchange chromatography [carboxymethyl (CM) cellulose]. The enzyme was 228-fold purified with 14.6% recovery. Analytical gel filtration chromatography on Sephadex G-200 co...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1131
更新日期:2014-03-01 00:00:00
abstract::Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1188
更新日期:2014-07-01 00:00:00
abstract::This review article focuses on the use of the methylotrophic yeast Pichia pastoris as a recombinant protein-expression system. P. pastoris is a useful system for the expression of milligram-to-gram quantities of a protein, which can be scaled up to fermentation to meet greater demands. Compared with mammalian cells, P...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:
更新日期:1999-10-01 00:00:00
abstract::The conformation of a protein refers to the three-dimensional arrangement of its constituent atoms. Since expression of the biological activity of a protein depends on its conformation, it is clear that full characterization of a protein involves an understanding of its three-dimensional structure. This review outline...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:10.1042/ba19990102
更新日期:2000-02-01 00:00:00
abstract::The use of glutaraldehyde (GTA) for cross-linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross-linking agent for tissue implants, we have evaluated a number of cross-linking procedures shown previousl...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::We tested a variety of liposomes for parameters such as DNA binding capacity and DNase I protection of incorporated and attached DNA to elucidate their use as vehicles for DNA transfer into cells and animals. The results were compared to other potential DNA vehicles, empty viral capsids, and nanoparticles. Maximal bin...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::The aim of the present study was to find disruption methods that allow fast and reproducible measurement of intracellular recombinant proteins with potential for on-line application. Production of rhSOD (recombinant human superoxide dismutase) by Escherichia coli was used as a model. Three methods of cell disruption, ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20030143
更新日期:2004-08-01 00:00:00
abstract::Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:10.1002/bab.1714
更新日期:2019-03-01 00:00:00
abstract::Colloidal carriers based on silica (Si) matrices are an innovative approach within the context of therapeutic drug delivery systems. These carriers are emerging as a great promise for diagnosis and treatment of a wide range of injuries, particularly in cancer and infectious diseases. In addition, bioencapsulation for ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:10.1002/bab.1322
更新日期:2015-11-01 00:00:00
abstract::Complex-formation between a truncated staphylococcal Protein A produced in Escherichia coli and a native E coli molecular chaperone, DnaK, can be used for the purification of DnaK by IgG-affinity chromatography. The half-time constant for in vitro formation of the Protein A-DnaK complex is about 14 min. Complex-format...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::We have constructed a hybrid protein (ATFHI) consisting of an N-terminal fragment from urokinase (ATF) and HI-8, which is the C-terminal domain of urinary trypsin inhibitor. The fusion genes for the hybrid proteins were engineered by PCR and cloned into expression plasmids. Under the control of the tac promoter, fusio...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Auricularia polytricha belonging to Basidiomycota has the ability to degrade lignocellulose. However, there has been no resource in public databases examining the transcriptome of A. polytricha. In this study, high-throughput sequencing platform BGISEQ-500 was used to generate large amount of transcript sequences from...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.2005
更新日期:2020-08-12 00:00:00
abstract::Second-generation bioethanol production process was developed using pretreated empty fruit bunches (EFB). Consecutive acid/alkali EFB pretreatment was performed, first with HCl and then with NaOH with final washing steps for phenolic compounds elimination. Scanning electron microscopy images showed that EFB chemical t...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1826
更新日期:2020-09-01 00:00:00
abstract::The main metabolic properties of human red blood cells (RBC) overloaded with glucose catabolizing enzymes such as hexokinase and glucose oxidase were evaluated. Human erythrocytes loaded with human hexokinase metabolized 3.1 +/- 0.2 mumol/h/ml RBC of glucose, an amount double that consumed by normal and unloaded cells...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1111/j.1470-8744.1992.tb00195.x
更新日期:1992-02-01 00:00:00
abstract::The membranotropic properties of block co-polymers and their protein conjugates were studied by their effect on the rate of oxygen consumption by isolated liver mitochondria and on thymus-derived lymphocytes. The block co-polymers consisted of poly(ethylene oxide) (PoE) [poly(ethylene glycol)] and poly(propylene oxide...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Cardiolipin, the primary lipid hapten in the antigen suspension used for the detection of antitreponemal antibodies in the sera of syphilitic patients, was successfully coupled to glucose oxidase, peroxidase, and some other enzymes using different crosslinking agents. These complexes were used to replace the pure unco...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::Human urokinase was immobilized on an ethylene vinyl acetate copolymer surface. Soluble urokinase showed its maximum activity at pH 8.5, while the immobilized enzyme was most active at pH 9.0. Apparently, the shift in optimal pH was due to the polyanionic nature of the carrier surface on which the enzyme was immobiliz...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
abstract::Tuberculosis has long been the scourge of humanity, claiming millions of lives. The family of PE_PGRS gene has been attributed to the Mycobacterium tuberculosis pathogenesis over the past few decades. The gene of PE_PGRS family proteins are most often clustered in a region of the genome often as overlapping genes and ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:10.1002/bab.1266
更新日期:2015-03-01 00:00:00
abstract::Some metabolic inhibitors, mevastatin (MVS), chlorocholine chloride (CCC), sodium pyrophosphate (NaPP) and D,L-glyceraldehyde (DLG), were respectively added into the suspension cultures of the yew Taxus chinensis var. mairei at the late phase of cell growth to study isopentenyl pyrophosphate (IPP) biosynthesis. The co...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/ba20020061
更新日期:2003-02-01 00:00:00
abstract::Penicillium ochrochloron Biourge, which was isolated from rotten sweet potato, can produce plant cell wall-degrading enzymes (PCWDEs) with high viscosity reducing capability for ethanol production using fresh sweet potato tubers as feedstock. The enzyme preparation was characterized by a broad enzyme spectrum includin...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1190
更新日期:2014-07-01 00:00:00
abstract::Oxidative DNA damage is involved in mutagenesis, carcinogenesis, aging, radiation effects and also in the action of several anticancer drugs. Accumulated evidence indicates that iron may play an important role in these processes. The conversion of the closed circular double-stranded supercoiled plasmid pcDNA3 into the...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20030047
更新日期:2003-12-01 00:00:00