Abstract:
:Cinnamyl acetate was synthesized using immobilized lipase through transesterification between ethyl acetate and cinnamyl alcohol. In the solvent-free system, ethyl acetate acted as not only the acyl donor but also as the solvent of cinnamyl alcohol. Conversion (90.06%) was achieved after 3 H when transesterification was carried out at ethyl acetate/cinnamyl alcohol 15:1, 2.67 g L of lipase (Novozym 435) loading, and 40°C. Excellent stability and reusability of the enzyme resulted from the moderate reaction system. Kinetic studies showed that the Michaelis constants for ethyl acetate and cinnamyl alcohol and the inhibition constant of cinnamyl alcohol were 2.241, 206.82, and 0.461 mmol L⁻¹, respectively, which indicated that the reaction complied with the Ping-Pong Bi-Bi mechanism, with the inhibition of cinnamyl alcohol on lipase.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Geng B,Wang M,Qi W,Su R,He Zdoi
10.1002/bab.1023subject
Has Abstractpub_date
2012-07-01 00:00:00pages
270-5issue
4eissn
0885-4513issn
1470-8744journal_volume
59pub_type
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