Identification of nacre matrix protein genes hic14 and hic19 and their roles in crystal growth and pearl formation in the mussel Hyriopsis cumingii.

abstract：:Second-generation bioethanol production process was developed using pretreated empty fruit bunches (EFB). Consecutive acid/alkali EFB pretreatment was performed, first with HCl and then with NaOH with final washing steps for phenolic compounds elimination. Scanning electron microscopy images showed that EFB chemical t...

journal_title：Biotechnology and applied biochemistry

authors： Campioni TS,Soccol CR,Libardi Junior N,Rodrigues C,Woiciechowski AL,Letti LAJ,Vandenberghe LPS

更新日期：2020-09-01 00:00:00

abstract：:We report a novel surface plasmon resonance (SPR) biosensor that uses the full-length Det7 phage tail protein (Det7T) to rapidly and selectively detect Salmonella enterica serovar Typhimurium (S. Typhimurium). Det7T, which was obtained using recombinant protein expression and purification in Escherichia coli, demonstr...

journal_title：Biotechnology and applied biochemistry

authors： Hyeon SH,Lim WK,Shin HJ

更新日期：2020-01-08 00:00:00

abstract：:Glutamate dehydrogenase (GDH) and alcohol dehydrogenase (ADH) have been encapsulated in sheep and human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At a fixed enzyme level in the dialysis bag (100 units/ml of RBCs), a significant encapsulation yield was observed for ADH, both in human ...

journal_title：Biotechnology and applied biochemistry

authors： Sanz S,Pinilla M,Garín M,Tipton KF,Luque J

更新日期：1995-10-01 00:00:00

abstract：:The present study was aimed to explore the molecular and structural features of UDP-N-acetylglucosamine pyrophosphorylase of Bombyx mori (BmUAP), an essential enzyme for chitin synthesis in insects. The BmUAP cDNA sequence was cloned and expression profiles were monitored during the molting and feeding stages of silkw...

journal_title：Biotechnology and applied biochemistry

authors： Palaka BK,Velmurugan Ilavarasi A,Sapam TD,Kotapati KV,Nallala VS,Khan MB,Ampasala DR

更新日期：2019-09-01 00:00:00

abstract：:In this study, various constructs and hosts were used to produce high levels of cecropin B2 (cecB2). To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin-binding domain and an intein self-cleavage motif in front of cecropin B2, without...

journal_title：Biotechnology and applied biochemistry

authors： Fang YT,Lai WS,Liu JH,Liu YC

更新日期：2019-03-01 00:00:00

abstract：:The number of approaches to recombinant protein production in plants is greater than ever before. Development of these new and improved technologies as production platforms for plant-made pharmaceuticals has and will continue to create new commercial opportunities in the pharmaceutical sector. However, it is inevitabl...

journal_title：Biotechnology and applied biochemistry

authors： Paul M,Ma JK

更新日期：2011-01-01 00:00:00

abstract：:The antifungal activity of iturin A and its interaction with erythrocyte membranes were enhanced in the presence of surfactin. The modification of the properties of iturin A was explained by the formation of mixed iturin A-surfactin micelles. Such mixed micelles were easily generated when both lipopeptides were in aqu...

journal_title：Biotechnology and applied biochemistry

authors： Thimon L,Peypoux F,Maget-Dana R,Roux B,Michel G

更新日期：1992-10-01 00:00:00

abstract：:Two different enzymes that metabolize lactate in the presence of oxygen, either to acetate plus CO2 (lactate 2-mono-oxygenase; Lmox) or to pyruvate plus H2O2 (lactate oxidase; Lox) were encapsulated in human and murine red blood cells (RBCs). Lmox shows a low affinity for lactate (Km 22 mM) and thus works at a low rat...

journal_title：Biotechnology and applied biochemistry

authors： Garin M,Rossi L,Luque J,Magnani M

更新日期：1995-12-01 00:00:00

abstract：:Ethylene glycol bis (beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA)-calcium buffer is widely used in various calcium-dependent reactions where free calcium concentrations of 1 microM or less are desirable. The free calcium concentration is calculated from the association constant of EGTA . Ca2- and serves as the ...

journal_title：Biotechnology and applied biochemistry

authors： Segal J

更新日期：1986-10-01 00:00:00

abstract：:Mouse band-3 crosslinked carrier erythrocytes have been prepared. [125I]Carbonic anhydrase (CA) has been encapsulated into mouse erythrocytes. Then, loaded erythrocytes were labelled with 51Cr. Eventually, these doubly labelled cells were crosslinked with band-3 crosslinking reagents. [125I]CA was shown to have cytoso...

journal_title：Biotechnology and applied biochemistry

authors： Jordán JA,Alvarez FJ,Tejedor MC,Díez JC

更新日期：1999-02-01 00:00:00

abstract：:Cell proliferation is a complex and important event in atherosclerosis, aging and cancer, and is under the control of signalling pathways. These signalling pathways in turn are effected by the presence of a number of chemicals. For this purpose, we have checked the effect of two chemicals on the proliferation of skin ...

journal_title：Biotechnology and applied biochemistry

authors： Onat D,Boscoboinik D,Azzi A,Basaga H

更新日期：1999-06-01 00:00:00

abstract：:In our work, one-step electro-deposition method was adopted to produce polyaniline (PANI) and functional multiwalled carbon nanotubes (f-MWCNTs) films on glass carbon electrodes, and the modified electrodes were applied as an electrochemical sensor for determination of 10-hydroxycamptothecine (10-HCPT). The f-MWCNTs w...

journal_title：Biotechnology and applied biochemistry

authors： Meng L,Dai J,Rong S,Gao H,Zhang Z,Zhang Y,Chang D,Pan H

更新日期：2020-09-24 00:00:00

abstract：:Tissue engineering has been conducted in the study of cardiovascular grafts for many years. Many obstacles have been overcome in this rapidly changing field, but one difficulty has remained until now: the large number of endothelial cells (ECs) needed for seeding the inner layer of bypass graft. Recent advances in end...

journal_title：Biotechnology and applied biochemistry

authors： Punshon G,Vara DS,Sales KM,Seifalian AM

更新日期：2011-09-01 00:00:00

abstract：:Microparticles have been used for delivery of chemo- and radio-therapeutic agents to tumours in patients for more than a decade, whereas cationic liposomes have been used for introducing nucleic acids into mammalian cells for the same period of time. This minireview discusses the potential of delivering therapeutic ON...

journal_title：Biotechnology and applied biochemistry

authors： Dass CR

更新日期：2004-10-01 00:00:00

abstract：:Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin inj...

journal_title：Biotechnology and applied biochemistry

authors： Calcabrini C,Mancini U,De Bellis R,Diaz AR,Martinelli M,Cucchiarini L,Sestili P,Stocchi V,Potenza L

更新日期：2017-05-01 00:00:00

abstract：:In the present study, the growth and key metabolic features of a gene-transfected Drosophila melanogaster (fruitfly) S2 (Schneider 2) cell population (S2AcRVGP cells), cultured in Sf900-II medium, have been evaluated to provide substantial support for the development of a bioprocess to produce RVGP (rabies-virus glyco...

journal_title：Biotechnology and applied biochemistry

authors： Swiech K,da Silva CS,Arantes MK,dos Santos AS,Astray RM,Pereira CA,Suazo CA

更新日期：2008-01-01 00:00:00

abstract：:A new system equipped with a computer-controlled multiple activity analyzer has been developed for the efficient purification of multiple enzymes. The system consists of the following units: conventional enzyme fractionation system with a peristaltic pump, liquid chromatographic column, fraction collector, and uv moni...

journal_title：Biotechnology and applied biochemistry

authors： Tokushige M,Sasaki S

更新日期：1986-12-01 00:00:00

abstract：:The urinary C-terminal telopeptide fragment of type II collagen (uCTX-II) has been reported as the efficient blood-based biomarker for osteoarthritis, which affects knees, hands, spine, and hips. This study reports a sensing strategy with antibody-conjugated gold nanoparticles (GNP) on an interdigitated electrode (IDE...

journal_title：Biotechnology and applied biochemistry

authors： Wang S,Su S,Yu C,Gopinath SCB,Yang Z

更新日期：2020-07-04 00:00:00

abstract：:Recently, hPNAS-4 [human PNAS-4; also known as C1orf121 (Genbank accession number NP-057160)] was reported to be a novel pro-apoptotic protein in mammalian cells. However, at present there is little information about hPNAS-4 and its molecular mechanisms. In our present studies, the hPNAS-4 gene was first cloned into t...

journal_title：Biotechnology and applied biochemistry

authors： Deng H,Jiang Q,Liang S,Yan F,Hou S,Qian Z,Li J,Wen Y,Yang J,Wei Y

更新日期：2010-02-11 00:00:00

abstract：:The human placenta is a rich raw material for production of many biopharmaceutical products. Here we describe a co-purification process for the production of four different proteins from haemolysed human placenta blood: IgG, catalase (Cat), superoxide dismutase (Sod) and albumin (Alb). The process can be divided in tw...

journal_title：Biotechnology and applied biochemistry

authors： Grellet S,Martins EA,Maimoni Gonçalves V,Yague Lopes AP,Raw I,Cabrera-Crespo J

更新日期：2001-12-01 00:00:00

abstract：:The conformation of a protein refers to the three-dimensional arrangement of its constituent atoms. Since expression of the biological activity of a protein depends on its conformation, it is clear that full characterization of a protein involves an understanding of its three-dimensional structure. This review outline...

journal_title：Biotechnology and applied biochemistry

authors： Price NC

更新日期：2000-02-01 00:00:00

abstract：:The development of lytic bacteriophages as therapeutic products is an attractive alternative to antibiotics. In this study, we evaluated the potential of phage tails for lysing Gram-positive bacteria. Phage P954, a well-characterized temperate staphylococcal phage, was found to adsorb to a large number of Staphylococc...

journal_title：Biotechnology and applied biochemistry

authors： Biddappa AC,Sundarrajan S,Ramalinga AB,Sriram B,Padmanabhan S

更新日期：2012-11-01 00:00:00

abstract：:Renal fibrosis results in the progressive renal dysfunction and leads to chronic kidney disease (CKD) and ultimately end-stage renal disease. Asiaticoside was reported to regulate synaptopodin, desmin, nephrin and podocin levels in adriamycin-induced nephropathy of rats. In this study, we found out that asiaticoside i...

journal_title：Biotechnology and applied biochemistry

authors： Zhang M,Liu S,Fang L,Wang G

更新日期：2021-01-14 00:00:00

abstract：:Three peptides complementary to exposed regions of two low-M(r) phosphotyrosine protein phosphatase isoenzymes and of the acylphosphatase muscle isoenzyme have been synthesized. Each peptide was synthesized on two different types of resins; the peptides were anchored to the resins by amide linkages. The peptide resins...

journal_title：Biotechnology and applied biochemistry

authors： Stefani M,Bucciantini M,Taddei N,Mincione G,Manao G,Ramponi G

更新日期：1994-10-01 00:00:00

abstract：:The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With ...

journal_title：Biotechnology and applied biochemistry

authors： Hou KC,Zaniewski R

更新日期：1990-06-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...

journal_title：Biotechnology and applied biochemistry

authors： Banks JG,Board RG,Sparks NH

更新日期：1986-04-01 00:00:00

abstract：:The hybridization of the native and chemically inactivated aspartase from Escherichia coli was studied. Preparations of the tetrameric enzyme obtained by mixing the native and N-ethylmaleimide (NEM)-inactivated aspartase in 4 M guanidine-HCl followed by 51-fold dilution at room temperature retained catalytic activity....

journal_title：Biotechnology and applied biochemistry

authors： Imaishi H,Yumoto N,Tokushige M

更新日期：1990-04-01 00:00:00

abstract：:The development of subunit vaccines is presently the main strategy being evaluated for prevention of infectious diseases. The use of recombinant-DNA techniques has facilitated the development of new principles for design and production of subunit vaccines. First of all, the properties of a target protein immunogen can...

journal_title：Biotechnology and applied biochemistry

authors： Hansson M,Nygren PA,Ståhl S

更新日期：2000-10-01 00:00:00

abstract：:The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatog...

journal_title：Biotechnology and applied biochemistry

authors： Rigi G,Ghaedmohammadi S,Ahmadian G

更新日期：2019-05-01 00:00:00