Abstract:
:In this study, various constructs and hosts were used to produce high levels of cecropin B2 (cecB2). To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin-binding domain and an intein self-cleavage motif in front of cecropin B2, without a His-tag, was best for cecB2 expression. E. coli ER2566 was the best host, and 2YT was the best medium for cultivation. Under these conditions, a cecB2 yield of 98.2 mg/L could be obtained after purification. The purified cecB2 expressed a wide antimicrobial effect on most Gram-negative strains, including multidrug-resistant Acinetobactor baumannii and Staphylococcus aureus. This study provides a systematic approach to the efficient production of the antimicrobial peptide (AMP) cecB2 via the recombinant E. coli process, which is expected to be an efficient way for the production of other AMPs.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Fang YT,Lai WS,Liu JH,Liu YCdoi
10.1002/bab.1716subject
Has Abstractpub_date
2019-03-01 00:00:00pages
209-215issue
2eissn
0885-4513issn
1470-8744journal_volume
66pub_type
杂志文章abstract::Two different enzymes that metabolize lactate in the presence of oxygen, either to acetate plus CO2 (lactate 2-mono-oxygenase; Lmox) or to pyruvate plus H2O2 (lactate oxidase; Lox) were encapsulated in human and murine red blood cells (RBCs). Lmox shows a low affinity for lactate (Km 22 mM) and thus works at a low rat...
journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1023
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1188
更新日期:2014-07-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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更新日期:2017-09-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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