Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544.

abstract：:Talpha1 (thymosin alpha 1) is important in treating immunodeficiency and other diseases. In order to study the feasibility of expressing Talpha1 in plants, as the first attempt, we designed and synthesized the Talpha1 gene according to the plant codon usage preference and constructed the 4xTalpha1 concatemer (four cop...

journal_title：Biotechnology and applied biochemistry

authors： Chen Y,Zhao L,Shen G,Cui L,Ren W,Zhang H,Qian H,Tang K

更新日期：2008-01-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs), the largest family of cell-surface molecules involved in a number of biological and pathological processes, have recently emerged as key players in carcinogenesis and cancer progression. Orphan G protein-coupled receptors (oGPCRs) are a group of proteins lacking endogenous ligands....

journal_title：Biotechnology and applied biochemistry

authors： Cui X,Liu Y,Wang B,Xian G,Liu X,Tian X,Qin C

更新日期：2015-11-01 00:00:00

abstract：:Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H2 , organic acids, ethanol, and cellulase has been explo...

journal_title：Biotechnology and applied biochemistry

authors： Kumar P,Maharjan A,Jun HB,Kim BS

更新日期：2019-03-01 00:00:00

abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...

journal_title：Biotechnology and applied biochemistry

authors： Banks JG,Board RG,Sparks NH

更新日期：1986-04-01 00:00:00

abstract：:Recently, the development of "green" methods for fabrication of silver nanoparticles (Ag-NPs) has been emphasized, in view of their environmental safety, feasibility, and low cost. In this study, a serine protease, EuP-82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl-NPs) i...

journal_title：Biotechnology and applied biochemistry

authors： Siritapetawee J,Limphirat W,Nantapong N,Songthamwat D

更新日期：2018-07-01 00:00:00

abstract：:In this study, the whole proteins from a Chinese three-striped box turtle (Cuora trifasciata) were extracted and hydrolyzed using three proteases (alcalase, papain, and protamex). By orthogonal experiments, the optimal hydrolysis conditions for producing peptides with the highest cancer cells growth inhibition activit...

journal_title：Biotechnology and applied biochemistry

authors： Mao X,He S,Zhang T,Guo X,Ge Y,Ma C,Zhang X

更新日期：2017-11-01 00:00:00

abstract：:The effect of silica nanoparticles and conventional silica sources on the changes in microbial biomass and silica availability to pure soil and maize rhizosphere was studied. Nanosilica (20-40 nm) was synthesized from rice husk and comprehensively characterized. The efficiency of nanosilica was evaluated in terms of i...

journal_title：Biotechnology and applied biochemistry

authors： Rangaraj S,Gopalu K,Rathinam Y,Periasamy P,Venkatachalam R,Narayanasamy K

更新日期：2014-11-01 00:00:00

abstract：:Monoclonal antibodies specific to human prolactin were generated by an improved hybridoma technique. Following immunization with hormone conjugated with hemocyanin and cell fusion, hybrid cells were first cultured in a semisolid medium containing methylcellulose and later transferred to liquid medium for subculture. O...

journal_title：Biotechnology and applied biochemistry

authors： Lee CY,Chow SN,Coleman P,DeTuri MP

更新日期：1987-02-01 00:00:00

abstract：:We have explored the adaptation of the cytochromes P450 (P450) of deep-sea bacteria to high hydrostatic pressures. Strict conservation of the protein fold and functional importance of protein-bound water make P450 a unique subject for the studies of high-pressure adaptation. Earlier, we expressed and purified a fatty-...

journal_title：Biotechnology and applied biochemistry

authors： Davydov DR,Sineva EV,Davydova NY,Bartlett DH,Halpert JR

更新日期：2013-01-01 00:00:00

abstract：:We report a novel surface plasmon resonance (SPR) biosensor that uses the full-length Det7 phage tail protein (Det7T) to rapidly and selectively detect Salmonella enterica serovar Typhimurium (S. Typhimurium). Det7T, which was obtained using recombinant protein expression and purification in Escherichia coli, demonstr...

journal_title：Biotechnology and applied biochemistry

authors： Hyeon SH,Lim WK,Shin HJ

更新日期：2020-01-08 00:00:00

abstract：:A deletion htpR mutant of Escherichia coli has been constructed on the basis of site-directed mutagenesis. To this end, the chromosomal allele of htpR gene was substituted by a mutant allele introduced into the cell with a recombinant plasmid. The htpR mutant is characterized by a reduced level of proteolysis and ther...

journal_title：Biotechnology and applied biochemistry

authors： Kiselev VI

更新日期：1988-10-01 00:00:00

abstract：:The pH optimum, temperature-dependence, thermal stability, substrate specificity and subsite affinities of the 66 kDa, pI 4.0 glucoamylase of the filamentous fungus Trichoderma reesei were determined. It had a pH optimum of 5.5 and a temperature optimum (5 min reaction time) of 70 degrees C with soluble starch as subs...

journal_title：Biotechnology and applied biochemistry

authors： Fagerström R,Kalkkinen N

更新日期：1995-04-01 00:00:00

abstract：:Nicotine is a potent parasympathomimetic stimulant and an important natural alkaloid mainly found in the Nicotiana genus of plants. It can directly threaten ecological security and human health in tobacco waste, wastewater, and other forms of tobacco production. Therefore, it is the basis of nicotine pollution prevent...

journal_title：Biotechnology and applied biochemistry

authors： Ruan A,Gao Y,Fang C,Xu Y

更新日期：2018-11-01 00:00:00

abstract：:Solubilization of 2',3',4',5'-tetrabenzoyl-5-acetyl-1,5-dihydroriboflavin (benzaflavin) by conjugates of BSA with poly(alkylene oxide)s [poly(ethylene glycol) and block co-polymers of ethylene oxide and propylene oxide (pluronics)] was investigated. Conjugates of BSA with pluronics were shown to have more solubilizing...

journal_title：Biotechnology and applied biochemistry

authors： Topchieva IN,Kurganov BI,Teplova MV,Gorskaya IA,Rudakova IP

更新日期：1993-06-01 00:00:00

abstract：:In the erythrocyte membrane, the mobility of band 3 protein, the receptor for concanavalin A (Con A), is drastically reduced by the membrane skeleton. Yet, the vesicles free of membrane skeletal proteins, isolated from the highly agglutinable proteinase-treated cells, are found to be devoid of Con A agglutinability. T...

journal_title：Biotechnology and applied biochemistry

authors： Pestonjamasp KN,Gokhale SM,Mehta NG

更新日期：1990-10-01 00:00:00

abstract：:To improve the thermostability of (R)-selective amine transaminase from Aspergillus terreus (AT-ATA), we used computer software Disulfide by Design and Modelling of Disulfide Bonds in Proteins to identify mutation sites where the disulfide bonds were most likely to form. We obtained three stabilized mutants (N25C-A28C...

journal_title：Biotechnology and applied biochemistry

authors： Xie DF,Fang H,Mei JQ,Gong JY,Wang HP,Shen XY,Huang J,Mei LH

更新日期：2018-03-01 00:00:00

abstract：:The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as...

journal_title：Biotechnology and applied biochemistry

authors： Cannio R,de Pascale D,Rossi M,Bartolucci S

更新日期：1994-04-01 00:00:00

abstract：:Parkinson's disease (PD) is the most common neurodegenerative movement disorder and is characterized by the loss of dopaminergic neurons and the formation of eosinophilic intracytoplasmic inclusion bodies known as Lewy bodies. Although alpha-synuclein is known to be a pivotal factor implicated in the pathogenesis of P...

journal_title：Biotechnology and applied biochemistry

authors： Choi JY,Sung YM,Park HJ,Hur EH,Lee SJ,Hahn C,Min BR,Kim IK,Kang S,Rhim H

更新日期：2002-08-01 00:00:00

abstract：:Single base mismatched oligonucleotides related to the rpoB gene of Mycobacterium tuberculosis, the mutations of which cause drug resistance of the infectious agent, were detected and discriminated using a surface plasmon resonance biosensor system. Thiol-modified oligonucleotides of the selected sequence (the probe) ...

journal_title：Biotechnology and applied biochemistry

authors： Rachkov A,Patskovsky S,Soldatkin A,Meunier M

更新日期：2013-07-01 00:00:00

abstract：:The combination of IL-2 (interleukin-2) and GM-CSF (granulocyte/macrophage colony-stimulating factor) has been broadly studied in antitumour immune therapy, but its efficacy is uncertain. To better exert the activities of the two cytokines and study them in a mouse model, we have constructed a bifunctional protein, hI...

journal_title：Biotechnology and applied biochemistry

authors： Wen Q,Ma L,Luo W,Zhou MQ,He D,Lin Y,Wu ZQ,He XW,Wang JF,Wang XN

更新日期：2008-05-01 00:00:00

abstract：:The dynamic gassing-out method was used to assess the effect of stirrer speed, air flow rate and alginate concentration on the overall volumetric oxygen mass transfer coefficient (K(L)a) in a bench-top stirred fermenter equipped with two Rushton-type turbines. As the alginate concentration in the medium was increased ...

journal_title：Biotechnology and applied biochemistry

authors： Parente E,Piraino P,Fidaleo M,Moresi M

更新日期：2004-10-01 00:00:00

abstract：:A pathway for the synthesis of the repeating tetrasaccharide units in gellan gum from Pseudomonas elodea is proposed. The enzymes presumed to be involved in the synthesis of the activated precursors UDP-glucose, TDP-rhamnose, and UDP-glucuronic acid were detected and assayed in crude cell extracts of the gellan-produc...

journal_title：Biotechnology and applied biochemistry

authors： Martins LO,Sá-Correia I

更新日期：1991-12-01 00:00:00

abstract：:St. John's wort ( Hypericum perforatum L.) is a herbaceous perennial distributed throughout the World that has been widely used in traditional medicine. H. perforatum produces several types of biologically active compound, including the hypericins--a family of light-activated anthraquinones, localized within specializ...

journal_title：Biotechnology and applied biochemistry

authors： Kirakosyan A,Sirvent TM,Gibson DM,Kaufman PB

更新日期：2004-02-01 00:00:00

abstract：:A new α-neoagarobiose hydrolase (NABH) called AgaWH117 was cloned from Agarivorans gilvus WH0801. The gene encoding this hydrolase consists of 1,086 bp and encodes a protein containing 361 amino acids. This new NABH showed 74% amino acid sequence identity with other known NABHs. The molecular mass of the recombinant A...

journal_title：Biotechnology and applied biochemistry

authors： Liu N,Yang M,Mao X,Mu B,Wei D

更新日期：2016-03-01 00:00:00

abstract：:As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...

journal_title：Biotechnology and applied biochemistry

authors： Hasegawa M,Kaku T,Kuroda M,Ise N,Kitano H

更新日期：1992-02-01 00:00:00

abstract：:Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...

journal_title：Biotechnology and applied biochemistry

authors： Li Q,Ng WT,Puah SM,Bhaskar RV,Soh LS,MacBeath C,Parakattil P,Green P,Wu JC

更新日期：2014-07-01 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is a hematologic malignancy characterized by excessive growth of myeloid cells and their progenitors. The proportion of spectrin dimers compared to tetramers extracted from membranes at 4 degrees C, under low ionic strength conditions, increased in CML erythrocytes. These also displa...

journal_title：Biotechnology and applied biochemistry

authors： Basu J,Kundu M,Rakshit MM,Chakrabarti P

更新日期：1990-10-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) still represents a major problem to the swine industry worldwide, causing high mortality rates in infected animals. Virus-like particles (VLPs) have gained attention for vaccine development, serving both as scaffolds for epitope expression and immune response enhancers. The commercial ...

journal_title：Biotechnology and applied biochemistry

authors： Aguilera BE,Chávez-Calvillo G,Elizondo-Quiroga D,Jimenez-García MN,Carrillo-Tripp M,Silva-Rosales L,Hernández-Gutiérrez R,Gutiérrez-Ortega A

更新日期：2017-05-01 00:00:00

abstract：:Entrapped cells and entrapped cells grown inside of a calcium alginate matrix as well as free cells have been investigated using Saccharomyces cerevisiae mutant strains with regard to their pattern of growth and invertase activity. The repression of invertase by glucose and glucose-consumption ability were considered ...

journal_title：Biotechnology and applied biochemistry

authors： Rossi-Alva JC,Rocha-Leão MH

更新日期：2003-08-01 00:00:00

abstract：:Among the heat-shock proteins (HSPs) induced in response to heat-shock (HS) in all organisms, the low-molecular-mass (LMM) HSPs are the most abundant and unique in plants. The HSP genes at the transcriptional and the translational levels have been well-characterized; however, relatively little is known about the physi...

journal_title：Biotechnology and applied biochemistry

authors： Yeh KW,Jinn TL,Yeh CH,Chen YM,Lin CY

更新日期：1994-02-01 00:00:00