Natural antimicrobial systems and their potential in food preservation of the future.

abstract：:Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and ...

journal_title：Biotechnology and applied biochemistry

authors： Karuppaiya P,Tsay HS

更新日期：2020-01-01 00:00:00

abstract：:We have analysed the status and transcriptional activity of the bovine papillomavirus-I (complete BPV-I genome)-based expression vector pCES in CI27i-cell-line-derived 3TI cells used for the industrial production of recombinant human erythropoietin (rhuEpo). Complete tandem head-to-tail integration of about 600 vector...

journal_title：Biotechnology and applied biochemistry

authors： Fibi MR,Kunz J,Weimer T,Schulz R,Teifke P,Grzeschik KH,Johannsen R,Zettlmeissl G

更新日期：1996-02-01 00:00:00

abstract：:The efficient enzyme-catalysed reaction of morpholines as model structures for bioactive compounds with para-dihydroxylated aromatic systems was carried out using the oxidoreductase laccase and atmospheric oxygen to produce eight novel morpholine-substituted aromatics. The laccase of Myceliophthora thermophila was use...

journal_title：Biotechnology and applied biochemistry

authors： Hahn V,Mikolasch A,Wende K,Bartrow H,Lindequist U,Schauer F

更新日期：2009-11-13 00:00:00

abstract：:HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...

journal_title：Biotechnology and applied biochemistry

authors： Vázquez-Blomquist D,González S,Duarte CA

更新日期：2002-12-01 00:00:00

abstract：:High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...

journal_title：Biotechnology and applied biochemistry

authors： Eriksson C,Schwenk JM,Sjöberg A,Hober S

更新日期：2010-06-09 00:00:00

abstract：:Biologically active membrane proteins are difficult to isolate. Very often the isolated membrane proteins have low binding affinity or no biological integrity at all. Despite some success in isolation, one has to overcome the hurdles of obtaining sufficient quantity of the proteins and maintaining biological activity ...

journal_title：Biotechnology and applied biochemistry

authors： Wong RB,Lee AH,Rangan VS,Cheng KC

更新日期：2009-05-01 00:00:00

abstract：:Cell proliferation is a complex and important event in atherosclerosis, aging and cancer, and is under the control of signalling pathways. These signalling pathways in turn are effected by the presence of a number of chemicals. For this purpose, we have checked the effect of two chemicals on the proliferation of skin ...

journal_title：Biotechnology and applied biochemistry

authors： Onat D,Boscoboinik D,Azzi A,Basaga H

更新日期：1999-06-01 00:00:00

abstract：:In our work, one-step electro-deposition method was adopted to produce polyaniline (PANI) and functional multiwalled carbon nanotubes (f-MWCNTs) films on glass carbon electrodes, and the modified electrodes were applied as an electrochemical sensor for determination of 10-hydroxycamptothecine (10-HCPT). The f-MWCNTs w...

journal_title：Biotechnology and applied biochemistry

authors： Meng L,Dai J,Rong S,Gao H,Zhang Z,Zhang Y,Chang D,Pan H

更新日期：2020-09-24 00:00:00

abstract：:The phosphatase and tensin homolog (PTEN) gene plays a crucial role in signal transduction by negatively regulating the PI3K signaling pathway. It is the most frequent mutated gene in many human-related cancers. Considering its critical role, a functional analysis of missense mutations of PTEN gene was undertaken in t...

journal_title：Biotechnology and applied biochemistry

authors： Khan I,Ansari IA,Singh P,Dass J FP

更新日期：2017-09-01 00:00:00

abstract：:A sensitive sandwich enzyme immunoassay (e.i.a.) for serrapeptase (TSP), an orally available anti-inflammatory proteinase, was established using affinity-purified anti-TSP rabbit IgG and its Fab' fragment conjugated with horseradish peroxidase as the first and the second antibodies respectively. TSP in the plasma was ...

journal_title：Biotechnology and applied biochemistry

authors： Moriya N,Nakata M,Nakamura M,Takaoka M,Iwasa S,Kato K,Kakinuma A

更新日期：1994-08-01 00:00:00

abstract：:Lipases are surface-active enzymes, acting on their substrates at the polar/nonpolar interface in emulsions. This study was aimed to test whether their activity, specificity, and the rates of formation/degradation of the various hydrolysis intermediates (i.e., mono- and diglycerides of interest as surface-active agent...

journal_title：Biotechnology and applied biochemistry

authors： Carpen A,Bonomi F,Iametti S,Marengo M

更新日期：2019-07-01 00:00:00

abstract：:2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a ...

journal_title：Biotechnology and applied biochemistry

authors： Lautié E,Fliniaux MA,Villarreal ML

更新日期：2010-03-12 00:00:00

abstract：:Some metabolic inhibitors, mevastatin (MVS), chlorocholine chloride (CCC), sodium pyrophosphate (NaPP) and D,L-glyceraldehyde (DLG), were respectively added into the suspension cultures of the yew Taxus chinensis var. mairei at the late phase of cell growth to study isopentenyl pyrophosphate (IPP) biosynthesis. The co...

journal_title：Biotechnology and applied biochemistry

authors： Wang YD,Yuan YJ,Lu M,Wu JC,Jiang JL

更新日期：2003-02-01 00:00:00

abstract：:The structural underpinnings of O₂ activation in P450s have been limited by the inability to trap unstable oxy complexes in the crystalline state for structure determination. To date there are only two known oxy P450 structures. Even so, much is known about O₂ activation in P450cam and the role that redox partner bind...

journal_title：Biotechnology and applied biochemistry

authors： Poulos TL,Madrona Y

更新日期：2013-01-01 00:00:00

abstract：:A simple, specific, sensitive and reproducible ELISA has been developed to quantify the level of CPS (capsular polysaccharide) production in supernatants of Streptococcus pneumoniae cell cultures. CPSs from Strep. pneumoniae have been widely used as vaccine antigens. The quantification method is based on two type-23F ...

journal_title：Biotechnology and applied biochemistry

authors： Cruz-Leal Y,Menéndez T,Coizeau E,Espinosa RR,Canaan L,Blanco F,Carmenate T,Chang J,Quiñones D,Tamargo I,Cremata J,Verez-Bencomo V,Guillén G

更新日期：2006-05-01 00:00:00

abstract：:Cinnamyl acetate was synthesized using immobilized lipase through transesterification between ethyl acetate and cinnamyl alcohol. In the solvent-free system, ethyl acetate acted as not only the acyl donor but also as the solvent of cinnamyl alcohol. Conversion (90.06%) was achieved after 3 H when transesterification w...

journal_title：Biotechnology and applied biochemistry

authors： Geng B,Wang M,Qi W,Su R,He Z

更新日期：2012-07-01 00:00:00

abstract：:Glutaraldehyde crosslinked, immunoprecipitated gulonolactone oxidase, injected intraperitoneally, has significant catalytic activity and is capable of providing long-term therapeutic benefit for the enzyme deficiency disease scurvy. The enzyme is tolerated even in repetitive doses. In the present study, however, we ha...

journal_title：Biotechnology and applied biochemistry

authors： Sato P,Lindemann D

更新日期：1986-04-01 00:00:00

abstract：:The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc....

journal_title：Biotechnology and applied biochemistry

authors： Takegawa K,Tohda H,Sasaki M,Idiris A,Ohashi T,Mukaiyama H,Giga-Hama Y,Kumagai H

更新日期：2009-06-22 00:00:00

abstract：:Among the heat-shock proteins (HSPs) induced in response to heat-shock (HS) in all organisms, the low-molecular-mass (LMM) HSPs are the most abundant and unique in plants. The HSP genes at the transcriptional and the translational levels have been well-characterized; however, relatively little is known about the physi...

journal_title：Biotechnology and applied biochemistry

authors： Yeh KW,Jinn TL,Yeh CH,Chen YM,Lin CY

更新日期：1994-02-01 00:00:00

abstract：:Higher thermostability or acid resistance for fungal α-amylase will help to improve the sugar-making process and cut down the production costs. Here, the thermostability or acid resistance of Rhizopus oryzae α-amylase (ROAmy) was significantly enhanced by site-directed evolution based on multiple sequence alignment (M...

journal_title：Biotechnology and applied biochemistry

authors： Li S,Yang Q,Tang B

更新日期：2020-07-01 00:00:00

abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...

journal_title：Biotechnology and applied biochemistry

authors： Hiragami F,Akiyama J,Koike Y,Kano Y

更新日期：2007-05-01 00:00:00

abstract：:The development of lytic bacteriophages as therapeutic products is an attractive alternative to antibiotics. In this study, we evaluated the potential of phage tails for lysing Gram-positive bacteria. Phage P954, a well-characterized temperate staphylococcal phage, was found to adsorb to a large number of Staphylococc...

journal_title：Biotechnology and applied biochemistry

authors： Biddappa AC,Sundarrajan S,Ramalinga AB,Sriram B,Padmanabhan S

更新日期：2012-11-01 00:00:00

abstract：:This review article focuses on the use of the methylotrophic yeast Pichia pastoris as a recombinant protein-expression system. P. pastoris is a useful system for the expression of milligram-to-gram quantities of a protein, which can be scaled up to fermentation to meet greater demands. Compared with mammalian cells, P...

journal_title：Biotechnology and applied biochemistry

authors： Fischer R,Drossard J,Emans N,Commandeur U,Hellwig S

更新日期：1999-10-01 00:00:00

abstract：:Tissue engineering has been conducted in the study of cardiovascular grafts for many years. Many obstacles have been overcome in this rapidly changing field, but one difficulty has remained until now: the large number of endothelial cells (ECs) needed for seeding the inner layer of bypass graft. Recent advances in end...

journal_title：Biotechnology and applied biochemistry

authors： Punshon G,Vara DS,Sales KM,Seifalian AM

更新日期：2011-09-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of garlic (Allium sativum L.) on the diabetic nephropathy and oxidative stress induced by STZ (streptozotocin) in rats. Diabetes was induced in Male Sprague-Dawley rats by administering a single intraperitoneal injection of STZ (60 mg/kg of body weight). ...

journal_title：Biotechnology and applied biochemistry

authors： Mariee AD,Abd-Allah GM,El-Yamany MF

更新日期：2009-03-01 00:00:00

abstract：:The α-amylase from soya bean seeds was purified by affinity precipitation, resulting in approx. 20-fold purification with approx. 84% recovery. The purified α-amylase had an optimum pH of 5.5, optimum temperature of 75 °C, Arrhenius energy of activation of 6.03 kcal/mol (1 kcal≈4.184 kJ) and a Km of 2.427 mg/ml (starc...

journal_title：Biotechnology and applied biochemistry

authors： Prakash O,Jaiswal N

更新日期：2010-12-01 00:00:00

abstract：:Mouse band-3 crosslinked carrier erythrocytes have been prepared. [125I]Carbonic anhydrase (CA) has been encapsulated into mouse erythrocytes. Then, loaded erythrocytes were labelled with 51Cr. Eventually, these doubly labelled cells were crosslinked with band-3 crosslinking reagents. [125I]CA was shown to have cytoso...

journal_title：Biotechnology and applied biochemistry

authors： Jordán JA,Alvarez FJ,Tejedor MC,Díez JC

更新日期：1999-02-01 00:00:00

abstract：:The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was ...

journal_title：Biotechnology and applied biochemistry

authors： Somkuti GA,Solaiman DK,Johnson TL,Steinberg DH

更新日期：1991-04-01 00:00:00

abstract：:The variation with pH of the kinetic parameters of penicillin acylase from Kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. The pH-dependence of log (V/Km) for penicillin G showed that a group with a pK value over 4.7 must be deprotonated and t...

journal_title：Biotechnology and applied biochemistry

authors： Martín J,Prieto I,Mancheño JM,Barbero JL,Arche R

更新日期：1993-06-01 00:00:00

abstract：:The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a plant species known for its production of TIAs (terpenoid indole alkaloids), many of which are pharmaceutically important. Ajmalicine and serpentine are prescribed for the treatment of hypertension, whereas the bisindoles vinblastine, vincristine and 3',...

journal_title：Biotechnology and applied biochemistry

authors： Zhou ML,Shao JR,Tang YX

更新日期：2009-04-01 00:00:00