The long-term stability in gene expression of human endothelial cells permits the production of large numbers of cells suitable for use in regenerative medicine.

Abstract:

:Tissue engineering has been conducted in the study of cardiovascular grafts for many years. Many obstacles have been overcome in this rapidly changing field, but one difficulty has remained until now: the large number of endothelial cells (ECs) needed for seeding the inner layer of bypass graft. Recent advances in endothelial progenitor cell (EPC) isolation and culture techniques have increased the interest in genetic studies. Despite these advances in EPC studies, the "gold standard" for the seeding of tissue engineering constructs or hybrid grafts remains mature human umbilical vein endothelial cells (HUVECs). This study investigates the ability of HUVECs to be expanded in culture to provide sufficient cells for graft seeding. The levels of gene expression of key genes are then examined to ensure that these cells retain the EC phenotype. This study demonstrates that HUVECs may be cultured for up to 12 passages without alteration in phenotype. Subsequent passage numbers are sufficiently similar to those preceding them to allow cells of different passages to be mixed without gene expression anomalies.

authors

Punshon G,Vara DS,Sales KM,Seifalian AM

doi

10.1002/bab.48

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

371-5

issue

5

eissn

0885-4513

issn

1470-8744

journal_volume

58

pub_type

杂志文章
  • Rapid purification of truncated Taq DNA polymerase Stoffel fragment by boiling lysis of bacterial expression cultures.

    abstract::A simplified and low-cost method, boiling lysis, was developed and used to purify the truncated DNA polymerase Stoffel fragment after overproduction of the enzyme in Escherichia coli. The method is based on the thermostable properties of the Stoffel fragment. Conditions such as the boiling time and number of cycles em...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1042/BA20070114

    authors: Yang Z,Ding Y,Zhang Y,Liu F

    更新日期:2008-06-01 00:00:00

  • Antisense peptides to the 43-57 region of acylphosphatase and to the 46-60 region of two isoenzymes of a low-M(r) phosphotyrosine protein phosphatase do not interact with the corresponding proteins.

    abstract::Three peptides complementary to exposed regions of two low-M(r) phosphotyrosine protein phosphatase isoenzymes and of the acylphosphatase muscle isoenzyme have been synthesized. Each peptide was synthesized on two different types of resins; the peptides were anchored to the resins by amide linkages. The peptide resins...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: Stefani M,Bucciantini M,Taddei N,Mincione G,Manao G,Ramponi G

    更新日期:1994-10-01 00:00:00

  • A quantitative human monoclonal antibody immunoassay using anti-idiotypic antibody as a membrane antigen surrogate with surface-plasmon-resonance detection.

    abstract::Biologically active membrane proteins are difficult to isolate. Very often the isolated membrane proteins have low binding affinity or no biological integrity at all. Despite some success in isolation, one has to overcome the hurdles of obtaining sufficient quantity of the proteins and maintaining biological activity ...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1042/BA20080072

    authors: Wong RB,Lee AH,Rangan VS,Cheng KC

    更新日期:2009-05-01 00:00:00

  • Carbamate kinase of Lactobacillus buchneri NCDO110. I. Purification and properties.

    abstract::Carbamate kinase has been prepared from Lactobacillus buchneri NCDO110. An approximately 91-fold increase in the specific activity of the enzyme was achieved. The purified extract exhibited a single band following polyacrylamide gel electrophoresis. The apparent molecular weight as determined by gel electrophoresis wa...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: Manca de Nadra MC,Nadra Chaud CA,Pesce de Ruiz Holgado A,Oliver G

    更新日期:1986-02-01 00:00:00

  • Sensitive determination of NT-proBNP for diagnosing abdominal aortic aneurysms incidence on interdigitated electrode sensor.

    abstract::Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.2006

    authors: Qiu C,Wang X,Zhang X,Li Z,Zhou Y,Kang J

    更新日期:2020-08-15 00:00:00

  • Melamine binding with arachidonic acid binding sites of albumin is a potential mechanism for melamine-induced inflammation.

    abstract::Melamine adulteration of food is a public health concern. It has been seen that melamine causes disease in many organs. Melamine-induced kidney disease is a well-recognized clinicopathological entity. Inflammation is thought to be important in melamine-induced pathology. Melamine is expected to bind with albumin becau...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1512

    authors: Rajpoot M,Bhattacharyya R,Banerjee D,Sharma A

    更新日期:2017-07-01 00:00:00

  • Argatroban-coupled Affi-Gel matrix for the purification of thrombin from plasma.

    abstract::Sometimes it is necessary to obtain thrombin from limited amounts of human plasma for laboratory assay. None of the available purification methods easily deals with this subject. The procedure described in the present paper uses a readily available pharmaceutical agent, argatroban, to construct an affinity matrix. Arg...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1042/BA20050057

    authors: Lefkowitz JB

    更新日期:2005-10-01 00:00:00

  • Status and transcriptional activity of a bovine-papillomavirus-I-based expression vector in a recombinant production cell line.

    abstract::We have analysed the status and transcriptional activity of the bovine papillomavirus-I (complete BPV-I genome)-based expression vector pCES in CI27i-cell-line-derived 3TI cells used for the industrial production of recombinant human erythropoietin (rhuEpo). Complete tandem head-to-tail integration of about 600 vector...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: Fibi MR,Kunz J,Weimer T,Schulz R,Teifke P,Grzeschik KH,Johannsen R,Zettlmeissl G

    更新日期:1996-02-01 00:00:00

  • Cloning, expression, and characterization of a novel sialidase from Brevibacterium casei.

    abstract::The sialidase gene from Brevibacterium casei was cloned in pET28a and overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3). The histidine-tagged sialidase protein was purified and characterized from the crude cell extracts of isopropyl-β-d-thiogalactopyranoside-induced cells using Ni-NTA agarose c...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1475

    authors: Wang X,Long H,Shen D,Liu L

    更新日期:2017-03-01 00:00:00

  • Rapid purification and analysis of alpha-synuclein proteins: C-terminal truncation promotes the conversion of alpha-synuclein into a protease-sensitive form in Escherichia coli.

    abstract::Parkinson's disease (PD) is the most common neurodegenerative movement disorder and is characterized by the loss of dopaminergic neurons and the formation of eosinophilic intracytoplasmic inclusion bodies known as Lewy bodies. Although alpha-synuclein is known to be a pivotal factor implicated in the pathogenesis of P...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1042/ba20020004

    authors: Choi JY,Sung YM,Park HJ,Hur EH,Lee SJ,Hahn C,Min BR,Kim IK,Kang S,Rhim H

    更新日期:2002-08-01 00:00:00

  • Immunodetection of urinary C-terminal telopeptide fragment of type II collagen: An osteoarthritis biomarker analysis.

    abstract::The urinary C-terminal telopeptide fragment of type II collagen (uCTX-II) has been reported as the efficient blood-based biomarker for osteoarthritis, which affects knees, hands, spine, and hips. This study reports a sensing strategy with antibody-conjugated gold nanoparticles (GNP) on an interdigitated electrode (IDE...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1981

    authors: Wang S,Su S,Yu C,Gopinath SCB,Yang Z

    更新日期:2020-07-04 00:00:00

  • Distinguishing transmembrane helices from peripheral helices by circular dichrosim.

    abstract::The interpretation of the circular dichroism (c.d.) spectra of proteins to date requires additional secondary structural information of the proteins to be analysed (e.g., X-ray or n.m.r. data). Therefore these methods are inappropriate for a c.d. database whose secondary structures are unknown, as in the case of the m...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Fasman GD

    更新日期:1993-10-01 00:00:00

  • Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples.

    abstract::High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1042/BA20100041

    authors: Eriksson C,Schwenk JM,Sjöberg A,Hober S

    更新日期:2010-06-09 00:00:00

  • Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica.

    abstract::Mirabilis himalaica is an endangered medicinal plant species in the Tibetan Plateau. The two genes respectively encoding chalcone synthase (MhCHS) and chalcone isomerase (MhCHI) were isolated and characterized from M. himalaica. The sequence analysis revealed that the two genes were similar with their corresponding ho...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1376

    authors: Lan X,Quan H,Xia X,Yin W,Zheng W

    更新日期:2016-05-01 00:00:00

  • Purification of glucoamylase by acarbose (BAY g-5421) affinity chromatography.

    abstract::Aspergillus niger and Rhizopus sp. glucoamylases were purified on an affinity chromatography column from commercially available, impure enzyme preparations. Up to 2 mg of glucoamylase protein was bound without leakage to a 1-ml affinity gel column (0.7 X 2.5 cm) possessing a covalently linked acarbose ligand (1 mg aca...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: Ono K,Smith EE

    更新日期:1986-04-01 00:00:00

  • A strategic study using mutant-strain entrapment in calcium alginate for the production of Saccharomyces cerevisiae cells with high invertase activity.

    abstract::Entrapped cells and entrapped cells grown inside of a calcium alginate matrix as well as free cells have been investigated using Saccharomyces cerevisiae mutant strains with regard to their pattern of growth and invertase activity. The repression of invertase by glucose and glucose-consumption ability were considered ...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1042/BA20020101

    authors: Rossi-Alva JC,Rocha-Leão MH

    更新日期:2003-08-01 00:00:00

  • One-step electrodeposition preparation of polyaniline/f-MWCNTs as electrochemical sensors for detection of 10-hydroxycamptothecine.

    abstract::In our work, one-step electro-deposition method was adopted to produce polyaniline (PANI) and functional multiwalled carbon nanotubes (f-MWCNTs) films on glass carbon electrodes, and the modified electrodes were applied as an electrochemical sensor for determination of 10-hydroxycamptothecine (10-HCPT). The f-MWCNTs w...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.2041

    authors: Meng L,Dai J,Rong S,Gao H,Zhang Z,Zhang Y,Chang D,Pan H

    更新日期:2020-09-24 00:00:00

  • Rabies virus production in non-woven polyester fabric(NWPF) packed-bed reactors.

    abstract::The production of rabies virus from baby hamster kidney-Ankara66 (BHK-An(66), HUKUK 99050302) monolayer cells was examinedin a packed-bed reactor containing non-woven polyester fabric (NWPF)discs. At first, growth characteristics of the cells were determined instatic culture. The suspension culture studies were realiz...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1042/ba20000052

    authors: Gümüşderelioğlu M,Aslankaraoğlu E,Gürhan SI

    更新日期:2001-06-01 00:00:00

  • Biobutanol production from sugarcane bagasse by Clostridium beijerinckii strains.

    abstract::Acetone-butanol-ethanol (ABE) fermentation was performed with sugarcane bagasse (SCB) hydrolysate using Clostridium beijerinckii strains. A cost-effective SCB medium was developed with no enzymatic hydrolysis and no supplementation of extra carbon source or expensive nitrogen source. One of the C. beijerinckii strains...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1865

    authors: Narayanasamy S,Chan KL,Cai H,Abdul Razak AHB,Tay BK,Miao H

    更新日期:2020-09-01 00:00:00

  • Increasing cytostatic effects of ricin A chain and Staphylococcus aureus enterotoxin A through in vitro hydrophobization with fatty acid residues.

    abstract::In order to impart an ability for receptor-independent transmembrane transfer to water-soluble proteins, it has been suggested that they be hydrophobized by lipid groups (fatty acids, etc.). To this end, systems of reversed micelles of surfactants in organic solvents were used as reaction media for protein modificatio...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: Alakhov VYu,Kabanov AV,Batrakova EV,Koromyslova IA,Levashov AV,Severin ES

    更新日期:1990-02-01 00:00:00

  • Isolation and characterization of antiproliferative peptides from Chinese three-striped box turtle (Cuora trifasciata).

    abstract::In this study, the whole proteins from a Chinese three-striped box turtle (Cuora trifasciata) were extracted and hydrolyzed using three proteases (alcalase, papain, and protamex). By orthogonal experiments, the optimal hydrolysis conditions for producing peptides with the highest cancer cells growth inhibition activit...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1548

    authors: Mao X,He S,Zhang T,Guo X,Ge Y,Ma C,Zhang X

    更新日期:2017-11-01 00:00:00

  • Endocytosis during the preparation of mouse and human carrier erythrocytes.

    abstract::Mouse and human erythrocytes are inherently different with respect to slow dialysis encapsulation used in preparing carrier erythrocytes. Fluorescein isothiocyanate (FITC)-dextran was added to five different stages of the encapsulation process to discern when endocytic vesiculation occurred. Mouse erythrocytes were mu...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: DeLoach JR,Droleskey RE

    更新日期:1993-08-01 00:00:00

  • Functional properties of proteins immobilized on albumin microspheres.

    abstract::Bovine serum albumin (BSA) microspheres with an average diameter of 12.5 micron were prepared by crosslinking of BSA molecules with glutaraldehyde in the presence of polymethylmethacrylate dissolved in chloroform-toluene. Trypsin and anti-human IgG antibody were immobilized onto their surfaces by the glutaraldehyde-ac...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: Yan CH,Kitano H,Ise N

    更新日期:1988-02-01 00:00:00

  • Application of statistical techniques for elucidating flow cytometric data of batch and fed-batch cultures.

    abstract::The objective of this work is to develop structured, segregated stochastic models for bioprocesses using time-series flow cytometric (FC) data. To this end, mammalian CHO cells were grown in both batch and fed-batch cultures, and their viable cell numbers (VCDs), monoclonal antibody (MAb), cell cycle phases, mitochond...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1138

    authors: Shirsat N,Avesh M,English NJ,Glennon B,Al-Rubeai M

    更新日期:2013-09-01 00:00:00

  • Cloning, overexpression, purification, and characterization of a new iron superoxide dismutase from Jatropha curcas.

    abstract::A novel iron superoxide dismutase (Fe-SOD) gene from Jatropha curcas was cloned and expressed in Escherichia coli BL21 (DE3). This recombinant enzyme was isolated by a combined procedure involving immobilized metal-ion affinity chromatography and ion-exchange chromatography. The apparent molecular mass of this purifie...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1030

    authors: Ou-Yang C,Cai F,Gao S,Niu B,Wang S,Chen F

    更新日期:2012-09-01 00:00:00

  • Molecular cloning and expression of a new α-neoagarobiose hydrolase from Agarivorans gilvus WH0801 and enzymatic production of 3,6-anhydro-l-galactose.

    abstract::A new α-neoagarobiose hydrolase (NABH) called AgaWH117 was cloned from Agarivorans gilvus WH0801. The gene encoding this hydrolase consists of 1,086 bp and encodes a protein containing 361 amino acids. This new NABH showed 74% amino acid sequence identity with other known NABHs. The molecular mass of the recombinant A...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1363

    authors: Liu N,Yang M,Mao X,Mu B,Wei D

    更新日期:2016-03-01 00:00:00

  • Composite of medium-chain-length polyhydroxyalkanoates-co-methyl acrylate and carbon nanotubes as innovative electrodes modifier in microbial fuel cell.

    abstract::A microbial fuel cell is a sustainable and environmental-friendly device that combines electricity generation and wastewater treatment through metabolic activities of microorganisms. However, low power output from inadequate electron transfer to the anode electrode hampers its practical implementation. Nanocomposites ...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1928

    authors: Sirajudeen AAO,Annuar MSM,Subramaniam R

    更新日期:2020-04-21 00:00:00

  • Biochemical characterization of a UDP-sugar pyrophosphorylase from Thermus caldophilus GK24.

    abstract::An extremely thermostable UDP-GlcNAc pyrophosphorylase has been purified from Thermus caldophilus GK24 by chromatographic methods including ion-exchange, hydrophobic interaction, and affinity chromatographies. The specific activity of the enzyme was enriched 41.8-fold, with a recovery of 2%. The molecular mass of the ...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:

    authors: Kim JS,Koh S,Shin HJ,Lee DS,Lee SY

    更新日期:1999-02-01 00:00:00

  • Isolation and characterization of a novel nicotinophilic bacterium, Arthrobacter sp. aRF-1 and its metabolic pathway.

    abstract::Nicotine is a potent parasympathomimetic stimulant and an important natural alkaloid mainly found in the Nicotiana genus of plants. It can directly threaten ecological security and human health in tobacco waste, wastewater, and other forms of tobacco production. Therefore, it is the basis of nicotine pollution prevent...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章

    doi:10.1002/bab.1682

    authors: Ruan A,Gao Y,Fang C,Xu Y

    更新日期:2018-11-01 00:00:00

  • Plant-made pharmaceuticals: leading products and production platforms.

    abstract::The number of approaches to recombinant protein production in plants is greater than ever before. Development of these new and improved technologies as production platforms for plant-made pharmaceuticals has and will continue to create new commercial opportunities in the pharmaceutical sector. However, it is inevitabl...

    journal_title:Biotechnology and applied biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/bab.6

    authors: Paul M,Ma JK

    更新日期:2011-01-01 00:00:00