Abstract：
:Enzymatic synthesis of biodiesel showed advantageous characteristics in relation to other technologies once it works under bland conditions, no generation of wastewater, no occurrence of saponifications reactions and production of a biodiesel with high quality. Although many researches still apply immobilized lipases, the high costs associated with this biocatalyst hamper the economic viability of the process. Lipases in free/soluble/liquid formulation employed to biodiesel production via hydroesterification reaction have attracted interest from researchers because they are more cost effective than the immobilized form, making the enzymatic route more competitive. In addition, soluble lipases present higher reaction rates, reducing the time required to obtain a satisfactory biodiesel yield. Despite the fact that already exist industrial plants producing biodiesel with the assistance of lipases in liquid formulation, results of researches show that the process still needs to overcome some drawbacks. This paper is a comprehensive and critical discussion on the publications where soluble lipases were applied on biodiesel synthesis, as well as the challenges that the technology faces and its current status in pilot and industrial applications.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
C Wancura JH,Tres MV,Jahn SL,de Oliveira JVdoi
10.1002/bab.1835subject
Has Abstractpub_date
2020-07-01 00:00:00pages
648-667issue
4eissn
0885-4513issn
1470-8744journal_volume
67pub_type
杂志文章,评审abstract：:An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferul...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:Mechano-growth factor (MGF), an alternative splicing variant of insulin-like growth factor-1 (IGF-1) gene, promotes cell proliferation and inhibits cell differentiation. It also plays an important role in tumor development. It is important to optimize the production process and achieve MGF protein because there is no ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1312
更新日期：2015-09-01 00:00:00
abstract：:The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:The structural underpinnings of O₂ activation in P450s have been limited by the inability to trap unstable oxy complexes in the crystalline state for structure determination. To date there are only two known oxy P450 structures. Even so, much is known about O₂ activation in P450cam and the role that redox partner bind...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1002/bab.1056
更新日期：2013-01-01 00:00:00
abstract：:Expression of the VP2 gene of autonomous parvoviruses in insect cells with the use of the baculovirus system has led to the production of virus-like particles (VLPs) formed by the self-assembly of VP2. These VLPs are expressed at high levels and can easily be purified by salt fractionation. They are highly immunogenic...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:The urinary C-terminal telopeptide fragment of type II collagen (uCTX-II) has been reported as the efficient blood-based biomarker for osteoarthritis, which affects knees, hands, spine, and hips. This study reports a sensing strategy with antibody-conjugated gold nanoparticles (GNP) on an interdigitated electrode (IDE...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1981
更新日期：2020-07-04 00:00:00
abstract：:Bovine serum albumin (BSA) microspheres with an average diameter of 12.5 micron were prepared by crosslinking of BSA molecules with glutaraldehyde in the presence of polymethylmethacrylate dissolved in chloroform-toluene. Trypsin and anti-human IgG antibody were immobilized onto their surfaces by the glutaraldehyde-ac...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:A kinetic model was developed to describe recombinant human protein disulphide isomerase (rhPDI)-assisted folding of a substrate protein, C125A recombinant human interleukin-2 (C125A rhIL-2). A series of progress curves showing native C125A rhIL-2 formation under different reaction conditions were generated. Non-linea...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20020057
更新日期：2003-04-01 00:00:00
abstract：:A new system equipped with a computer-controlled multiple activity analyzer has been developed for the efficient purification of multiple enzymes. The system consists of the following units: conventional enzyme fractionation system with a peristaltic pump, liquid chromatographic column, fraction collector, and uv moni...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1188
更新日期：2014-07-01 00:00:00
abstract：:A three-layered simulation methodology is described that rapidly evaluates biomanufacturing process options. In each layer, inferior options are screened out, while more promising candidates are evaluated further in the subsequent, more refined layer, which uses more rigorous models that require more data from time-co...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20070018
更新日期：2007-10-01 00:00:00
abstract：:The effects of chitin [(1-->4)-2-acetamido-2-deoxy-beta-D-glucan] and its partially deacetylated derivatives, chitosans, on the human dermal fibroblast-mediated contraction of collagen lattices were examined in vitro as a model for the contraction of cutaneous wounds in vivo. Chitosan CL313A, a short-chain-length 89% ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20020040
更新日期：2002-12-01 00:00:00
abstract：:The structural gene of elastase strain K (elastase from Pseudomonas aeruginosa strain K), namely HindIII1500PstI, was successfully sequenced to contain 1497 bp. The amino acid sequence, deduced from the nucleotide sequence, revealed that the mature elastase consists of 301 amino acids, with a molecular mass of 33.1 kD...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20100224
更新日期：2010-09-01 00:00:00
abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1986-04-01 00:00:00
abstract：:The application of the sulfhydryl protease ficin as biocatalyst is proposed as a novel method for enzyme-catalyzed synthesis of dipeptides. The negligible peptidase but considerable esterase activity at alkaline pH facilitated the kinetically controlled formation of peptide bonds by coupling the ester substrates Z-Ala...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1111/j.1470-8744.1992.tb00195.x
更新日期：1992-02-01 00:00:00
abstract：:The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20000065
更新日期：2001-02-01 00:00:00
abstract：:The human placenta is a rich raw material for production of many biopharmaceutical products. Here we describe a co-purification process for the production of four different proteins from haemolysed human placenta blood: IgG, catalase (Cat), superoxide dismutase (Sod) and albumin (Alb). The process can be divided in tw...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20010023
更新日期：2001-12-01 00:00:00
abstract：:Flat panels are the most spread type of photobioreactors for studying light effects on a microalgae culture. Their low thickness, usually between 1 and 3 cm, aims at ensuring light homogeneity across the culture. Yet because optical density has to remain very low, studies are still limited to low cell density cultures...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1894
更新日期：2020-02-03 00:00:00
abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20060200
更新日期：2007-05-01 00:00:00
abstract：:Mouse band-3 crosslinked carrier erythrocytes have been prepared. [125I]Carbonic anhydrase (CA) has been encapsulated into mouse erythrocytes. Then, loaded erythrocytes were labelled with 51Cr. Eventually, these doubly labelled cells were crosslinked with band-3 crosslinking reagents. [125I]CA was shown to have cytoso...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:An extremely thermostable UDP-GlcNAc pyrophosphorylase has been purified from Thermus caldophilus GK24 by chromatographic methods including ion-exchange, hydrophobic interaction, and affinity chromatographies. The specific activity of the enzyme was enriched 41.8-fold, with a recovery of 2%. The molecular mass of the ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1999-02-01 00:00:00
abstract：:Most insulin-dependent diabetes mellitus patients gen-erate conformational autoantibodies to the islet-cell 65-kDa variant of human glutamate decarboxylase (GAD65), and several immunochemical tests for the early detection of type-1 diabetes rely on GAD65 antibody (GADA) assessment using properly folded recombinant GAD...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba19990103
更新日期：2000-06-01 00:00:00
abstract：:Although most PCRs would produce proper PCR products when first tried, a general optimization is required to yield the best results. This optimization is often achieved by changing one factor at a time. However, this may lead to suboptimal results, since interactions between conditions are difficult to detect with thi...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/BA20040192
更新日期：2005-10-01 00:00:00
abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20020001
更新日期：2002-06-01 00:00:00
abstract：:Synthetic membranes containing 10% acrylamide units were subjected to activation with formaldehyde at pH 7.5 and 45 degrees C. Trypsin, invertase, and urease were bound to this activated membrane and the kinetic properties of immobilized enzymes were studied. The permeability of the membrane for distilled water manife...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:Agrobacterium rhizogenes-mediated hairy-root cultures are a very promising alternative to the biotechnological exploitation of plant cell cultures. Their characteristic capacity for secondary-metabolite production, inherent genetic stability reflected in stable productivity and the possibility of genetic manipulation ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
doi：10.1042/BA20070103
更新日期：2008-01-01 00:00:00
abstract：:The protective antigen (PA) component of anthrax toxin mediates delivery of either lethal factor (LF) or oedema factor into the cytosol of mammalian cells. The N-terminal domain of LF(1-254) (amino acids 1-254 of LF) binds to PA and, when fused to heterologous proteins, delivers such proteins into the cytosol. In the ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20000037
更新日期：2000-08-01 00:00:00
abstract：:Melamine adulteration of food is a public health concern. It has been seen that melamine causes disease in many organs. Melamine-induced kidney disease is a well-recognized clinicopathological entity. Inflammation is thought to be important in melamine-induced pathology. Melamine is expected to bind with albumin becau...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1512
更新日期：2017-07-01 00:00:00
abstract：:Ethylene glycol bis (beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA)-calcium buffer is widely used in various calcium-dependent reactions where free calcium concentrations of 1 microM or less are desirable. The free calcium concentration is calculated from the association constant of EGTA . Ca2- and serves as the ...
journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
