Abstract:
:The effects of chitin [(1-->4)-2-acetamido-2-deoxy-beta-D-glucan] and its partially deacetylated derivatives, chitosans, on the human dermal fibroblast-mediated contraction of collagen lattices were examined in vitro as a model for the contraction of cutaneous wounds in vivo. Chitosan CL313A, a short-chain-length 89% deacetylated chitosan chloride, inhibited fibroblast-populated collagen lattice (FPCL) contraction at higher initial concentrations (500 and 1,000 microg/ml) in FPCLs fabricated with responsive dermal fibroblasts, while in FPCLs containing non-responsive fibroblasts inhibition of contraction was reduced. The responsive and non-responsive phenotype of human dermal fibroblasts to treatment with chitosan CL313A has been reported previously by us. The inhibition of fibroblast-mediated collagen lattice contraction by chitosan appeared to be strongly correlated with whether the cells were responsive or non-responsive. The effect of chitin-50A on fibroblast-mediated collagen lattice contraction was also examined to investigate whether the level of deacetylation was important for its inhibitory effect on contraction. However, this had no effect on contraction at the concentrations tested, supporting previous work that only chitosan samples with higher levels of deacetylation showed any biological activity. This work indicates that highly deacetylated chitosan inhibits fibroblast-mediated contraction of collagen lattices and may therefore be useful as a therapeutic agent to reduce contraction and therefore scarring in wound healing in vivo.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Howling GI,Dettmar PW,Goddard PA,Hampson FC,Dornish M,Wood EJdoi
10.1042/ba20020040subject
Has Abstractpub_date
2002-12-01 00:00:00pages
247-53issue
3eissn
0885-4513issn
1470-8744journal_volume
36pub_type
杂志文章abstract::The protective antigen (PA) component of anthrax toxin mediates delivery of either lethal factor (LF) or oedema factor into the cytosol of mammalian cells. The N-terminal domain of LF(1-254) (amino acids 1-254 of LF) binds to PA and, when fused to heterologous proteins, delivers such proteins into the cytosol. In the ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/ba20000037
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abstract::Sometimes it is necessary to obtain thrombin from limited amounts of human plasma for laboratory assay. None of the available purification methods easily deals with this subject. The procedure described in the present paper uses a readily available pharmaceutical agent, argatroban, to construct an affinity matrix. Arg...
journal_title:Biotechnology and applied biochemistry
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abstract::HIV stimulates strong immune CD8(+) cytotoxic T lymphocytes (CTL) response in infected people, despite causing an immunodeficiency. It has been demonstrated that this response could be very important for the control of the virus. We have shown previously that a recombinant fowlpox virus (rFWPV), expressing the multi-e...
journal_title:Biotechnology and applied biochemistry
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doi:10.1042/ba20020027
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abstract::Enzymatic synthesis of biodiesel showed advantageous characteristics in relation to other technologies once it works under bland conditions, no generation of wastewater, no occurrence of saponifications reactions and production of a biodiesel with high quality. Although many researches still apply immobilized lipases,...
journal_title:Biotechnology and applied biochemistry
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abstract::The plant polymer lignin is the greatest source of aromatic chemical structures on earth. Hence, the chemically diverse lignin monomers are valuable raw materials for fine chemicals, materials synthesis, and food and flavor industries. However, extensive use of this natural resource is hampered by the large number of ...
journal_title:Biotechnology and applied biochemistry
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abstract::Three peptides complementary to exposed regions of two low-M(r) phosphotyrosine protein phosphatase isoenzymes and of the acylphosphatase muscle isoenzyme have been synthesized. Each peptide was synthesized on two different types of resins; the peptides were anchored to the resins by amide linkages. The peptide resins...
journal_title:Biotechnology and applied biochemistry
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abstract::Second-generation bioethanol production process was developed using pretreated empty fruit bunches (EFB). Consecutive acid/alkali EFB pretreatment was performed, first with HCl and then with NaOH with final washing steps for phenolic compounds elimination. Scanning electron microscopy images showed that EFB chemical t...
journal_title:Biotechnology and applied biochemistry
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abstract::The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...
journal_title:Biotechnology and applied biochemistry
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abstract::Ethylene glycol bis (beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA)-calcium buffer is widely used in various calcium-dependent reactions where free calcium concentrations of 1 microM or less are desirable. The free calcium concentration is calculated from the association constant of EGTA . Ca2- and serves as the ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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doi:10.1042/BA20020101
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journal_title:Biotechnology and applied biochemistry
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更新日期:1988-02-01 00:00:00
abstract::Naproxen and bezafibrate fall into the category of pharmaceuticals that have been widely detected in the aquatic environment, and one of the major sources is the effluent discharge from wastewater treatment plants. This study investigated the sorption and degradation kinetics of naproxen and bezafibrate in the presenc...
journal_title:Biotechnology and applied biochemistry
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更新日期:2014-05-01 00:00:00
abstract::Succinylcholine apnea happens in cases of null butyrylcholinesterase activity after administration of preintubation succinylcholine. So far, there is no such popular test that can rapidly screen null butyrylcholinesterase activity from plasma. Development of a novel method for rapid screening of null butyrylcholineste...
journal_title:Biotechnology and applied biochemistry
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doi:10.1002/bab.1252
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journal_title:Biotechnology and applied biochemistry
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更新日期:2019-07-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
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abstract::An inducible esterase has been isolated from a liquid culture of Aspergillus niger grown on sugar-beet pulp. The enzyme was active on methyl esters of cinnamic acids, caffeic > p-coumaric > ferulic, and is therefore termed a cinnamoyl esterase. The enzyme was not active on methyl sinapinate, a good substrate for ferul...
journal_title:Biotechnology and applied biochemistry
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doi:
更新日期:1996-06-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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更新日期:1999-06-01 00:00:00
abstract::Colloidal carriers based on silica (Si) matrices are an innovative approach within the context of therapeutic drug delivery systems. These carriers are emerging as a great promise for diagnosis and treatment of a wide range of injuries, particularly in cancer and infectious diseases. In addition, bioencapsulation for ...
journal_title:Biotechnology and applied biochemistry
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doi:10.1002/bab.1322
更新日期:2015-11-01 00:00:00
abstract::The α-amylase from soya bean seeds was purified by affinity precipitation, resulting in approx. 20-fold purification with approx. 84% recovery. The purified α-amylase had an optimum pH of 5.5, optimum temperature of 75 °C, Arrhenius energy of activation of 6.03 kcal/mol (1 kcal≈4.184 kJ) and a Km of 2.427 mg/ml (starc...
journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:
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abstract::Fermentation of herb Polygonum hydropiper L. (PHL) and cassava pulp (CP) for feed additive production with simultaneous flavonoid dissolution was investigated, and a two-stage response surface methodology (RSM) based on Plackett-Burman factorial design (PB design) was used to optimize the flavonoid dissolution and pro...
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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doi:
更新日期:1998-04-01 00:00:00
abstract::A rapid, simple and sensitive fluorometric assay method for the determination of nitrilase activity is described. 3-Cyanopyridine was hydrolysed to nicotinic acid by Rhodococcus rhodochrous and the liberated NH(3) was allowed to react with buffered o -phthaldialdehyde-2-mercaptoethanol solution (pH 7.4) to form a fluo...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20020106
更新日期:2003-06-01 00:00:00
abstract::Auricularia polytricha belonging to Basidiomycota has the ability to degrade lignocellulose. However, there has been no resource in public databases examining the transcriptome of A. polytricha. In this study, high-throughput sequencing platform BGISEQ-500 was used to generate large amount of transcript sequences from...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.2005
更新日期:2020-08-12 00:00:00
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journal_title:Biotechnology and applied biochemistry
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更新日期:2003-10-01 00:00:00
abstract::Catalytic properties of Escherichia coli polyphosphate kinase (EC 2.7.4.1), a promising enzyme for use in ATP regeneration (Hoffman, et al., 1988, Biotechnol. Appl. Biochem. 10, 107-117), are reported here. E. coli polyphosphate kinase (PPK) is broadly active in the pH range 5.5 to 8.5, having an optimal Vmax at pH 7....
journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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journal_title:Biotechnology and applied biochemistry
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更新日期:1993-12-01 00:00:00
abstract::The dynamic gassing-out method was used to assess the effect of stirrer speed, air flow rate and alginate concentration on the overall volumetric oxygen mass transfer coefficient (K(L)a) in a bench-top stirred fermenter equipped with two Rushton-type turbines. As the alginate concentration in the medium was increased ...
journal_title:Biotechnology and applied biochemistry
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