Abstract：
:Entrapped cells and entrapped cells grown inside of a calcium alginate matrix as well as free cells have been investigated using Saccharomyces cerevisiae mutant strains with regard to their pattern of growth and invertase activity. The repression of invertase by glucose and glucose-consumption ability were considered in the selection process of the mutants. Efficient sucrose bioconversion due to high invertase activity was obtained when entrapped mutant strain Q6R2 cells were grown within calcium alginate gel beads using sucrose plus glucose as the carbon source. Under these conditions, 1 mg (dry weight) of entrapped cells is able to produce 20 micromol of inverted sugar in 3 min (the maximum activity obtained was 20 units x mg(-1)). The experiments were carried out for 6 months without appreciable loss of either bead integrity or invertase activity. The biocatalyst was also stored at 4 degrees C for 6 months without appreciable loss of the invertase activity. This work shows that entrapped yeast cells with a weak ability to consume sugar may be used to produce inverted sugar.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Rossi-Alva JC,Rocha-Leão MHdoi
10.1042/BA20020101subject
Has Abstractpub_date
2003-08-01 00:00:00pages
43-51issue
Pt 1eissn
0885-4513issn
1470-8744pii
BA20020101journal_volume
38pub_type
杂志文章abstract：:As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, there is currently a lack of simple and effective method for quantitative detection...
journal_title：Biotechnology and applied biochemistry
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journal_title：Biotechnology and applied biochemistry
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journal_title：Biotechnology and applied biochemistry
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doi：
更新日期：1986-10-01 00:00:00
abstract：:The sialidase gene from Brevibacterium casei was cloned in pET28a and overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3). The histidine-tagged sialidase protein was purified and characterized from the crude cell extracts of isopropyl-β-d-thiogalactopyranoside-induced cells using Ni-NTA agarose c...
journal_title：Biotechnology and applied biochemistry
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doi：10.1002/bab.1475
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
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journal_title：Biotechnology and applied biochemistry
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更新日期：2003-10-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
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更新日期：2004-06-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.43
更新日期：2011-11-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1042/ba20010022
更新日期：2001-08-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：10.1002/bab.1981
更新日期：2020-07-04 00:00:00
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journal_title：Biotechnology and applied biochemistry
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更新日期：2010-12-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章,评审
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更新日期：2000-02-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
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更新日期：2003-06-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
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doi：
更新日期：1986-02-01 00:00:00
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更新日期：2010-05-24 00:00:00
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journal_title：Biotechnology and applied biochemistry
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doi：
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journal_title：Biotechnology and applied biochemistry
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更新日期：2014-07-01 00:00:00
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更新日期：2009-03-01 00:00:00
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journal_title：Biotechnology and applied biochemistry
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更新日期：2015-07-01 00:00:00
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更新日期：2009-11-13 00:00:00
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journal_title：Biotechnology and applied biochemistry
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
