Abstract:
:A protein with a molecular mass of 66 kDa was isolated by a simple, rapid, and inexpensive method, using 3-N-morpholinopropanesulfonic acid, potassium thiocyanate, and dithiothreitol, from a mixture of spores, parasporal crystals, and cell debris of Bacillus thuringiensis subsp. kurstaki. The protein was active against the third instar larvae of Trichoplusia ni, was soluble in 19 mM Na2CO3, and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and confirmed as the insecticidal component of the 132-kDa protoxin of B. thuringiensis subsp. kurstaki by an enzyme-linked immunosorbent assay using antibodies prepared against the protoxin.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Venkateswerlu G,Stotzky Gsubject
Has Abstractpub_date
1990-06-01 00:00:00pages
245-51issue
3eissn
0885-4513issn
1470-8744journal_volume
12pub_type
杂志文章abstract::The hybridization of the native and chemically inactivated aspartase from Escherichia coli was studied. Preparations of the tetrameric enzyme obtained by mixing the native and N-ethylmaleimide (NEM)-inactivated aspartase in 4 M guanidine-HCl followed by 51-fold dilution at room temperature retained catalytic activity....
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Two monoclonal antibodies against human myeloperoxidase, designated 3-2H3 and 4-2C11, were produced and characterized. Both bound to the native enzyme, but neither bound to the denatured enzyme or to its two denatured subunits. 4-2C11 bound to the three types of leukocyte myeloperoxidase, I, II, and III, as well as to...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1042
更新日期:2012-11-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1749
更新日期:2019-07-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:10.1002/bab.2101
更新日期:2021-01-12 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1761
更新日期:2019-07-01 00:00:00
abstract::Future developments in gene therapy and DNA vaccination depend on cost-effective large-scale production of pharmaceutical-grade pDNA (plasmid DNA). Given the large amount of impurities present in the feedstock, purification processes that have high specificity and capacity at a moderate cost are required. In the prese...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20040107
更新日期:2005-08-01 00:00:00
abstract::A pathway for the synthesis of the repeating tetrasaccharide units in gellan gum from Pseudomonas elodea is proposed. The enzymes presumed to be involved in the synthesis of the activated precursors UDP-glucose, TDP-rhamnose, and UDP-glucuronic acid were detected and assayed in crude cell extracts of the gellan-produc...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Most large-scale microbial production of recombinant proteins are based on Escherichia coli, yeasts, or filamentous fungi systems. Using eukaryotic hosts, antibody fragments are generally expressed by targeting to the secretory pathway. This enables not only efficient disulfide bond formation but also secretion of sol...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.67
更新日期:2012-01-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1013
更新日期:2012-05-01 00:00:00
abstract::Production of His-BbST [hexahistidine-tagged BbST (bubaline somatotropin)] by auto-induction in high-density shake-flask cultures coupled with a single-step, on-column purification and refolding strategy is described here. To optimize expression of BbST, different media and expression conditions were tested. The highe...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20060154
更新日期:2007-05-01 00:00:00
abstract::The L-arginine uptake of resting platelets from type-2 diabetic patients and control subjects was measured and the kinetic parameters defined. The effect of platelet stimulation with agonists was also investigated. Kinetic studies showed that the K(m) value for L-arginine transport was not different in patients compar...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/ba20010022
更新日期:2001-08-01 00:00:00
abstract::The use of glutaraldehyde (GTA) for cross-linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross-linking agent for tissue implants, we have evaluated a number of cross-linking procedures shown previousl...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1188
更新日期:2014-07-01 00:00:00
abstract::The participation of Mg2+ or Mn2+ nucleoside diphosphates in the reverse reaction catalyzed by purified carbamate kinase (ATP:carbamate phosphotransferase, EC 2.7.2.2) of Lactobacillus buchneri NCDO110 was studied. The results of initial velocity studies have indicated that Mn2+ ADP is as effective as a substrate as M...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract::So far, many studies have focused on biodegradable scaffolds for tissue engineering purposes. Herein, a starch-based biodegradable scaffold was fabricated by the freeze-drying method and cross-linked using a different concentration of 3-glycidoxypropyl-trimethoxysilane (GPTMS). Field emission scanning electron microsc...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1694
更新日期:2019-01-01 00:00:00
abstract::Metal prostheses of artificial joints undergo wear, producing numerous metal particles and ions, such as Cr3+ . Cr3+ is considered a key factor leading to aseptic loosening. Many studies focus on the effect of Cr3+ on osteoblasts; however, little is known about the effect of Cr3+ on the B-cell maturation antigen (BCMA...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1401
更新日期:2017-09-01 00:00:00
abstract::[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20100041
更新日期:2010-06-09 00:00:00
abstract::The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Sephadex was derivatized consecutively with Staphylococcus Protein A (SpA) and cell-specific antibodies, and the binding of cells to the resulting material was examined. For comparison, cell binding to commercially obtained SpA-Sepharose was determined. Sephadex G-10, carboxylated by reaction with glycine and activate...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/ba20010062
更新日期:2002-02-01 00:00:00
abstract::Marine biomass, especially the algal biomass, is currently considered to be one of the most potential candidates for biofuels conversion during the development of biomass utilization. In this study, a diluted sulfuric acid pretreatment method was established for biobutanol from red algal biomass Gelidium amansii using...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1820
更新日期:2020-09-01 00:00:00
abstract::G-protein-coupled receptors (GPCRs), the largest family of cell-surface molecules involved in a number of biological and pathological processes, have recently emerged as key players in carcinogenesis and cancer progression. Orphan G protein-coupled receptors (oGPCRs) are a group of proteins lacking endogenous ligands....
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1326
更新日期:2015-11-01 00:00:00
abstract::A truncated precursor form of prostate-specific antigen (PSA), [-2]proPSA, is a well-known biomarker for prostate cancer. To develop a biomarker assay, highly purified [-2]proPSA is required as a standard reference and for generation of a specific antibody. In this study, we generated an efficient mammalian expression...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1481
更新日期:2017-05-01 00:00:00
abstract::The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:
更新日期:1986-04-01 00:00:00
abstract::Succinylcholine apnea happens in cases of null butyrylcholinesterase activity after administration of preintubation succinylcholine. So far, there is no such popular test that can rapidly screen null butyrylcholinesterase activity from plasma. Development of a novel method for rapid screening of null butyrylcholineste...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1252
更新日期:2015-03-01 00:00:00
abstract::Diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase has been isolated previously using classical protein isolation techniques [A. Guranowski and S. Blanquet (1985) J. Biol. Chem. 260, 3542-3547]. A protein A-Sepharose immunoaffinity column was prepared to simplify the purification procedure. The immunoaffini...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::The aspartic proteinase gene of Mucor pusillus rennin expressed in Pichia pastoris was characterized in terms of structural and conformational stability induced by temperature. This enzyme is 12% glycosylated, with a similar specific activity to the native fungal enzyme. The secondary structure determined by CD is mai...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/ba19990094
更新日期:2000-04-01 00:00:00
abstract::Agrobacterium rhizogenes-mediated hairy-root cultures are a very promising alternative to the biotechnological exploitation of plant cell cultures. Their characteristic capacity for secondary-metabolite production, inherent genetic stability reflected in stable productivity and the possibility of genetic manipulation ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:10.1042/BA20070103
更新日期:2008-01-01 00:00:00
abstract::Solubilization of 2',3',4',5'-tetrabenzoyl-5-acetyl-1,5-dihydroriboflavin (benzaflavin) by conjugates of BSA with poly(alkylene oxide)s [poly(ethylene glycol) and block co-polymers of ethylene oxide and propylene oxide (pluronics)] was investigated. Conjugates of BSA with pluronics were shown to have more solubilizing...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00