Abstract:
:Solubilization of 2',3',4',5'-tetrabenzoyl-5-acetyl-1,5-dihydroriboflavin (benzaflavin) by conjugates of BSA with poly(alkylene oxide)s [poly(ethylene glycol) and block co-polymers of ethylene oxide and propylene oxide (pluronics)] was investigated. Conjugates of BSA with pluronics were shown to have more solubilizing efficiency towards benzaflavin than BSA itself. Solubilized forms of benzaflavin are able to inhibit NADPH-dependent peroxidation of lipids in rat liver microsomes. A study of solubilized riboflavin ester transfer to mitochondria demonstrated that conjugates of BSA with pluronics may be advantageously employed for this purpose. Inhibitory properties of solubilized forms of benzaflavin were demonstrated by the study of their influence on the enzyme activity of D-amino-acid oxidase from pig kidney. The results show that solubilized forms of biologically active compounds based on conjugates of proteins with poly(alkylene oxide)s may be used for testing of chemical substances in biochemical systems.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Topchieva IN,Kurganov BI,Teplova MV,Gorskaya IA,Rudakova IPsubject
Has Abstractpub_date
1993-06-01 00:00:00pages
337-48issue
3eissn
0885-4513issn
1470-8744journal_volume
17pub_type
杂志文章abstract::The aim of this paper is to make the point on the fortieth years study on the β-glycosidase from Sulfolobus solfataricus. This enzyme represents one of the thermophilic biocatalysts, which is more extensively studied as witnessed by the numerous literature reports available since 1980. Comprehensive biochemical studie...
journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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journal_title:Biotechnology and applied biochemistry
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doi:
更新日期:1999-08-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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更新日期:1992-10-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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doi:
更新日期:1992-04-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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更新日期:1990-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-03-12 00:00:00
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journal_title:Biotechnology and applied biochemistry
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更新日期:1996-02-01 00:00:00
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更新日期:1999-06-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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更新日期:2004-10-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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journal_title:Biotechnology and applied biochemistry
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更新日期:1990-10-01 00:00:00
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journal_title:Biotechnology and applied biochemistry
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更新日期:1999-12-01 00:00:00
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