Purification and characterization of a novel esterase induced by growth of Aspergillus niger on sugar-beet pulp.

abstract：:Marine biomass, especially the algal biomass, is currently considered to be one of the most potential candidates for biofuels conversion during the development of biomass utilization. In this study, a diluted sulfuric acid pretreatment method was established for biobutanol from red algal biomass Gelidium amansii using...

journal_title：Biotechnology and applied biochemistry

authors： Hong Y,Chen C,Wu YR

更新日期：2020-09-01 00:00:00

abstract：:The introduction of different methodologies for the construction and screening of complex protein libraries has provided powerful means in protein engineering for the development of molecules with desired traits. A challenge faced in many situations is to adapt a given methodology for efficient and rapid identificatio...

journal_title：Biotechnology and applied biochemistry

authors： Löfdahl PA,Nygren PA

更新日期：2010-03-05 00:00:00

abstract：:Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new ti...

journal_title：Biotechnology and applied biochemistry

authors： Tien HT,Salamon Z

更新日期：1990-10-01 00:00:00

abstract：:Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in de...

journal_title：Biotechnology and applied biochemistry

authors： Chen L,Guo S,Wu L,Hao C,Xu W,Zhang J

更新日期：2015-05-01 00:00:00

abstract：:The membranotropic properties of block co-polymers and their protein conjugates were studied by their effect on the rate of oxygen consumption by isolated liver mitochondria and on thymus-derived lymphocytes. The block co-polymers consisted of poly(ethylene oxide) (PoE) [poly(ethylene glycol)] and poly(propylene oxide...

journal_title：Biotechnology and applied biochemistry

authors： Kirillova GP,Mokhova EN,Dedukhova VI,Tarakanova AN,Ivanova VP,Efremova NV,Topchieva IN

更新日期：1993-12-01 00:00:00

abstract：:The present study elaborates a cost-effective and transfectant-free method for generating recombinant Bombyx mori (silkworm) nucleopolyhedrovirus in silkworm larvae and pupae by injecting invasive Escherichia coli carrying BmBacmid [BmNPV (B. mori nucleopolyhedrovirus)-Bacmid] into larval haemocoel. Up to 109 PFU (pla...

journal_title：Biotechnology and applied biochemistry

authors： Sun J,Yao L,Yao N,Xu H,Jin P,Kan Y

更新日期：2010-12-01 00:00:00

abstract：:Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrog...

journal_title：Biotechnology and applied biochemistry

authors： Chirila TV,Suzuki S,Papolla C

更新日期：2017-11-01 00:00:00

abstract：:Cell proliferation is a complex and important event in atherosclerosis, aging and cancer, and is under the control of signalling pathways. These signalling pathways in turn are effected by the presence of a number of chemicals. For this purpose, we have checked the effect of two chemicals on the proliferation of skin ...

journal_title：Biotechnology and applied biochemistry

authors： Onat D,Boscoboinik D,Azzi A,Basaga H

更新日期：1999-06-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:A microbial fuel cell is a sustainable and environmental-friendly device that combines electricity generation and wastewater treatment through metabolic activities of microorganisms. However, low power output from inadequate electron transfer to the anode electrode hampers its practical implementation. Nanocomposites ...

journal_title：Biotechnology and applied biochemistry

authors： Sirajudeen AAO,Annuar MSM,Subramaniam R

更新日期：2020-04-21 00:00:00

abstract：:The purpose of the present study was to investigate the effects of garlic (Allium sativum L.) on the diabetic nephropathy and oxidative stress induced by STZ (streptozotocin) in rats. Diabetes was induced in Male Sprague-Dawley rats by administering a single intraperitoneal injection of STZ (60 mg/kg of body weight). ...

journal_title：Biotechnology and applied biochemistry

authors： Mariee AD,Abd-Allah GM,El-Yamany MF

更新日期：2009-03-01 00:00:00

abstract：:Mechano-growth factor (MGF), an alternative splicing variant of insulin-like growth factor-1 (IGF-1) gene, promotes cell proliferation and inhibits cell differentiation. It also plays an important role in tumor development. It is important to optimize the production process and achieve MGF protein because there is no ...

journal_title：Biotechnology and applied biochemistry

authors： Feng J,Wan R,Yi Q,He L,Yang L,Tang L

更新日期：2015-09-01 00:00:00

abstract：:The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently, we reported that a de novo carbene transferase, a tetra-α-helical c-type heme-containing protein, C45, is proficient at N-H insertion reactions, proceeding via the intermolecular transfer of a metallocarben...

journal_title：Biotechnology and applied biochemistry

authors： Stenner R,Anderson JLR

更新日期：2020-07-01 00:00:00

abstract：:Yeast cytochrome c peroxidase (CcP) was purified from baker's yeast and immobilised onto a nylon membrane. The kinetics of the soluble and immobilised forms of the enzyme were investigated for the catalysed oxidation of potassium ferrocyanide in the presence of H2O2 and m-chloroperoxybenzoic acid. The pH dependence of...

journal_title：Biotechnology and applied biochemistry

authors： Cooper JM,McNeil CJ,Bannister JV

更新日期：1991-08-01 00:00:00

abstract：:The hybridization of the native and chemically inactivated aspartase from Escherichia coli was studied. Preparations of the tetrameric enzyme obtained by mixing the native and N-ethylmaleimide (NEM)-inactivated aspartase in 4 M guanidine-HCl followed by 51-fold dilution at room temperature retained catalytic activity....

journal_title：Biotechnology and applied biochemistry

authors： Imaishi H,Yumoto N,Tokushige M

更新日期：1990-04-01 00:00:00

abstract：:The combination of IL-2 (interleukin-2) and GM-CSF (granulocyte/macrophage colony-stimulating factor) has been broadly studied in antitumour immune therapy, but its efficacy is uncertain. To better exert the activities of the two cytokines and study them in a mouse model, we have constructed a bifunctional protein, hI...

journal_title：Biotechnology and applied biochemistry

authors： Wen Q,Ma L,Luo W,Zhou MQ,He D,Lin Y,Wu ZQ,He XW,Wang JF,Wang XN

更新日期：2008-05-01 00:00:00

abstract：:The variation with pH of the kinetic parameters of penicillin acylase from Kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. The pH-dependence of log (V/Km) for penicillin G showed that a group with a pK value over 4.7 must be deprotonated and t...

journal_title：Biotechnology and applied biochemistry

authors： Martín J,Prieto I,Mancheño JM,Barbero JL,Arche R

更新日期：1993-06-01 00:00:00

abstract：:Aspergillus niger and Rhizopus sp. glucoamylases were purified on an affinity chromatography column from commercially available, impure enzyme preparations. Up to 2 mg of glucoamylase protein was bound without leakage to a 1-ml affinity gel column (0.7 X 2.5 cm) possessing a covalently linked acarbose ligand (1 mg aca...

journal_title：Biotechnology and applied biochemistry

authors： Ono K,Smith EE

更新日期：1986-04-01 00:00:00

abstract：:The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was ...

journal_title：Biotechnology and applied biochemistry

authors： Somkuti GA,Solaiman DK,Johnson TL,Steinberg DH

更新日期：1991-04-01 00:00:00

abstract：:Amylin has been implicated in type II diabetes because of its inherent property to misfold into toxic aggregates. Although it has been shown that amylin interacts with cell membranes, no study to date has monitored the association process using a direct approach. The present study uses confocal microscopy to identify ...

journal_title：Biotechnology and applied biochemistry

authors： Pillay K,Govender P

更新日期：2013-07-01 00:00:00

abstract：:As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, there is currently a lack of simple and effective method for quantitative detection...

journal_title：Biotechnology and applied biochemistry

authors： Chen C,Liang H,Hu B,Ning B,Lai H,He Y,Guo G,Zhong S,Li L

更新日期：2020-06-13 00:00:00

abstract：:This review article focuses on the use of the methylotrophic yeast Pichia pastoris as a recombinant protein-expression system. P. pastoris is a useful system for the expression of milligram-to-gram quantities of a protein, which can be scaled up to fermentation to meet greater demands. Compared with mammalian cells, P...

journal_title：Biotechnology and applied biochemistry

authors： Fischer R,Drossard J,Emans N,Commandeur U,Hellwig S

更新日期：1999-10-01 00:00:00

abstract：:In the erythrocyte membrane, the mobility of band 3 protein, the receptor for concanavalin A (Con A), is drastically reduced by the membrane skeleton. Yet, the vesicles free of membrane skeletal proteins, isolated from the highly agglutinable proteinase-treated cells, are found to be devoid of Con A agglutinability. T...

journal_title：Biotechnology and applied biochemistry

authors： Pestonjamasp KN,Gokhale SM,Mehta NG

更新日期：1990-10-01 00:00:00

abstract：:St. John's wort ( Hypericum perforatum L.) is a herbaceous perennial distributed throughout the World that has been widely used in traditional medicine. H. perforatum produces several types of biologically active compound, including the hypericins--a family of light-activated anthraquinones, localized within specializ...

journal_title：Biotechnology and applied biochemistry

authors： Kirakosyan A,Sirvent TM,Gibson DM,Kaufman PB

更新日期：2004-02-01 00:00:00

abstract：:In this study, various constructs and hosts were used to produce high levels of cecropin B2 (cecB2). To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin-binding domain and an intein self-cleavage motif in front of cecropin B2, without...

journal_title：Biotechnology and applied biochemistry

authors： Fang YT,Lai WS,Liu JH,Liu YC

更新日期：2019-03-01 00:00:00

abstract：:As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...

journal_title：Biotechnology and applied biochemistry

authors： Hasegawa M,Kaku T,Kuroda M,Ise N,Kitano H

更新日期：1992-02-01 00:00:00

abstract：:Nicotine is a potent parasympathomimetic stimulant and an important natural alkaloid mainly found in the Nicotiana genus of plants. It can directly threaten ecological security and human health in tobacco waste, wastewater, and other forms of tobacco production. Therefore, it is the basis of nicotine pollution prevent...

journal_title：Biotechnology and applied biochemistry

authors： Ruan A,Gao Y,Fang C,Xu Y

更新日期：2018-11-01 00:00:00

abstract：:Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H2 , organic acids, ethanol, and cellulase has been explo...

journal_title：Biotechnology and applied biochemistry

authors： Kumar P,Maharjan A,Jun HB,Kim BS

更新日期：2019-03-01 00:00:00

abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...

journal_title：Biotechnology and applied biochemistry

authors： Qiu C,Wang X,Zhang X,Li Z,Zhou Y,Kang J

更新日期：2020-08-15 00:00:00

abstract：:In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carra...

journal_title：Biotechnology and applied biochemistry

authors： Koklukaya SZ,Sezer S,Aksoy S,Hasirci N

更新日期：2016-09-01 00:00:00