Effects of recombinant IL-17F intranasal inoculation against Streptococcus pneumoniae infection in a murine model.

abstract：:Neuroblastoma (NB) is an embryonic solid tumor derived from precursor cells of the sympathetic nervous system, and accounts for 11% of childhood cancers and around 15% of cancer deaths in children. SUMOylation and deSUMOylation are dynamic mechanisms regulating a spectrum of protein activities. The SUMO proteases (SEN...

journal_title：Biotechnology and applied biochemistry

authors： Xiang-Ming Y,Zhi-Qiang X,Ting Z,Jian W,Jian P,Li-Qun Y,Ming-Cui F,Hong-Liang X,Xu C,Yun Z

更新日期：2016-05-01 00:00:00

abstract：:As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, there is currently a lack of simple and effective method for quantitative detection...

journal_title：Biotechnology and applied biochemistry

authors： Chen C,Liang H,Hu B,Ning B,Lai H,He Y,Guo G,Zhong S,Li L

更新日期：2020-06-13 00:00:00

abstract：:Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for bo...

journal_title：Biotechnology and applied biochemistry

authors： Parascandola P,de Alteriis E

更新日期：1996-02-01 00:00:00

abstract：:So far, many studies have focused on biodegradable scaffolds for tissue engineering purposes. Herein, a starch-based biodegradable scaffold was fabricated by the freeze-drying method and cross-linked using a different concentration of 3-glycidoxypropyl-trimethoxysilane (GPTMS). Field emission scanning electron microsc...

journal_title：Biotechnology and applied biochemistry

authors： Aidun A,Zamanian A,Ghorbani F

更新日期：2019-01-01 00:00:00

abstract：:Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin inj...

journal_title：Biotechnology and applied biochemistry

authors： Calcabrini C,Mancini U,De Bellis R,Diaz AR,Martinelli M,Cucchiarini L,Sestili P,Stocchi V,Potenza L

更新日期：2017-05-01 00:00:00

abstract：:Among the heat-shock proteins (HSPs) induced in response to heat-shock (HS) in all organisms, the low-molecular-mass (LMM) HSPs are the most abundant and unique in plants. The HSP genes at the transcriptional and the translational levels have been well-characterized; however, relatively little is known about the physi...

journal_title：Biotechnology and applied biochemistry

authors： Yeh KW,Jinn TL,Yeh CH,Chen YM,Lin CY

更新日期：1994-02-01 00:00:00

abstract：:Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrog...

journal_title：Biotechnology and applied biochemistry

authors： Chirila TV,Suzuki S,Papolla C

更新日期：2017-11-01 00:00:00

abstract：:Second-generation bioethanol production process was developed using pretreated empty fruit bunches (EFB). Consecutive acid/alkali EFB pretreatment was performed, first with HCl and then with NaOH with final washing steps for phenolic compounds elimination. Scanning electron microscopy images showed that EFB chemical t...

journal_title：Biotechnology and applied biochemistry

authors： Campioni TS,Soccol CR,Libardi Junior N,Rodrigues C,Woiciechowski AL,Letti LAJ,Vandenberghe LPS

更新日期：2020-09-01 00:00:00

abstract：:The therapeutic potential of microRNA-21 (miR-21) small-molecule inhibitors has been of particular interest to medicinal chemists. Moreover, the development of more facile screening methods is lacking. In the present study, two potential screening strategies for miR-21 small-molecule inhibitor including the stem-loop ...

journal_title：Biotechnology and applied biochemistry

authors： Hei YY,Guo YX,Jiang CS,Wang S,Lu SM,Zhang SQ

更新日期：2019-09-01 00:00:00

abstract：:An amylase-producing psychrotroph bacterium was isolated from soil and identified as belonging to the genus Exiguobacterium. A novel cold-adapted α-amylase, Amy SH3, was purified from culture medium of this bacterium using acetone precipitation and DEAE-Sepharose anion-exchange chromatography. The molecular mass of th...

journal_title：Biotechnology and applied biochemistry

authors： Mojallali L,Shahbani Zahiri H,Rajaei S,Akbari Noghabi K,Haghbeen K

更新日期：2014-03-01 00:00:00

abstract：:The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...

journal_title：Biotechnology and applied biochemistry

authors： Son HJ,Heo MS,Kim YG,Lee SJ

更新日期：2001-02-01 00:00:00

abstract：:High yields of extracellular alpha-galactosidase from fungal cultures were obtained by inducing enzyme production with guar gum (a galactomannan obtained from the seeds of Cyamopsis tetragonobola) as the sole carbon source. An alpha-galactosidase was isolated from the culture medium of Penicillium ochrochloron culture...

journal_title：Biotechnology and applied biochemistry

authors： Dey PM,Patel S,Brownleader MD

更新日期：1993-06-01 00:00:00

abstract：:The aim of the present study was to find disruption methods that allow fast and reproducible measurement of intracellular recombinant proteins with potential for on-line application. Production of rhSOD (recombinant human superoxide dismutase) by Escherichia coli was used as a model. Three methods of cell disruption, ...

journal_title：Biotechnology and applied biochemistry

authors： Tkac J,Vostiar I,Mandenius CF

更新日期：2004-08-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:Succinylcholine apnea happens in cases of null butyrylcholinesterase activity after administration of preintubation succinylcholine. So far, there is no such popular test that can rapidly screen null butyrylcholinesterase activity from plasma. Development of a novel method for rapid screening of null butyrylcholineste...

journal_title：Biotechnology and applied biochemistry

authors： Nagpal N,Chowdhary S,Bhattacharyya R,Banerjee D

更新日期：2015-03-01 00:00:00

abstract：:Marine biomass, especially the algal biomass, is currently considered to be one of the most potential candidates for biofuels conversion during the development of biomass utilization. In this study, a diluted sulfuric acid pretreatment method was established for biobutanol from red algal biomass Gelidium amansii using...

journal_title：Biotechnology and applied biochemistry

authors： Hong Y,Chen C,Wu YR

更新日期：2020-09-01 00:00:00

abstract：:A simplified and low-cost method, boiling lysis, was developed and used to purify the truncated DNA polymerase Stoffel fragment after overproduction of the enzyme in Escherichia coli. The method is based on the thermostable properties of the Stoffel fragment. Conditions such as the boiling time and number of cycles em...

journal_title：Biotechnology and applied biochemistry

authors： Yang Z,Ding Y,Zhang Y,Liu F

更新日期：2008-06-01 00:00:00

abstract：:Expression of the VP2 gene of autonomous parvoviruses in insect cells with the use of the baculovirus system has led to the production of virus-like particles (VLPs) formed by the self-assembly of VP2. These VLPs are expressed at high levels and can easily be purified by salt fractionation. They are highly immunogenic...

journal_title：Biotechnology and applied biochemistry

authors： Casal JI

更新日期：1999-04-01 00:00:00

abstract：:Optison(R) is an ultrasound contrast agent, consisting of gas-filled microspheres surrounded by a solid shell of heat-denatured human albumin. Size-distribution measurements of these microspheres are a critical stability indicating factor, because loss of encapsulated gas eliminates ultrasound contrast activity. Compo...

journal_title：Biotechnology and applied biochemistry

authors： Podell S,Burrascano C,Gaal M,Golec B,Maniquis J,Mehlhaff P

更新日期：1999-12-01 00:00:00

abstract：:The ability of Acidithiobacillus ferrooxidans to oxidize ferrous iron has been extensively studied in bioleaching to recover metal resources. Although immobilization of A. ferrooxidans is of great importance to achieve high bioleaching performance in practical application, the reported approaches of immobilization of ...

journal_title：Biotechnology and applied biochemistry

authors： Zhu N,Shi C,Shang R,Yang C,Xu Z,Wu P

更新日期：2017-09-01 00:00:00

abstract：:Biologically active membrane proteins are difficult to isolate. Very often the isolated membrane proteins have low binding affinity or no biological integrity at all. Despite some success in isolation, one has to overcome the hurdles of obtaining sufficient quantity of the proteins and maintaining biological activity ...

journal_title：Biotechnology and applied biochemistry

authors： Wong RB,Lee AH,Rangan VS,Cheng KC

更新日期：2009-05-01 00:00:00

abstract：:Dysosma pleiantha (Hance) Woodson is one of the endangered traditional Chinese medicinal herbs, highly valued for its medicinal properties by Taiwan's mountain tribes. The present study aims to develop an efficient protocol for callus biomass by optimizing suitable culture medium, carbon source culture condition, and ...

journal_title：Biotechnology and applied biochemistry

authors： Karuppaiya P,Tsay HS

更新日期：2020-01-01 00:00:00

abstract：:Tuberculosis has long been the scourge of humanity, claiming millions of lives. The family of PE_PGRS gene has been attributed to the Mycobacterium tuberculosis pathogenesis over the past few decades. The gene of PE_PGRS family proteins are most often clustered in a region of the genome often as overlapping genes and ...

journal_title：Biotechnology and applied biochemistry

authors： Meena LS

更新日期：2015-03-01 00:00:00

abstract：:A rapid, simple and sensitive fluorometric assay method for the determination of nitrilase activity is described. 3-Cyanopyridine was hydrolysed to nicotinic acid by Rhodococcus rhodochrous and the liberated NH(3) was allowed to react with buffered o -phthaldialdehyde-2-mercaptoethanol solution (pH 7.4) to form a fluo...

journal_title：Biotechnology and applied biochemistry

authors： Banerjee A,Sharma R,Banerjee UC

更新日期：2003-06-01 00:00:00

abstract：:Pancreatic duodenum homeobox protein-1 (PDX1) is a master regulatory gene in pancreatic development. Reprogramming of mesenchymal stem cells (MSCs) is a promising tool for producing insulin-producing cells. In this study, lentivirus harboring PDX1 (LV-PDX1) has been used for persistence gene expression in MSCs. The ob...

journal_title：Biotechnology and applied biochemistry

authors： Talebi S,Aleyasin A,Soleimani M,Massumi M

更新日期：2012-05-01 00:00:00

abstract：:The application of the sulfhydryl protease ficin as biocatalyst is proposed as a novel method for enzyme-catalyzed synthesis of dipeptides. The negligible peptidase but considerable esterase activity at alkaline pH facilitated the kinetically controlled formation of peptide bonds by coupling the ester substrates Z-Ala...

journal_title：Biotechnology and applied biochemistry

authors： Monter B,Herzog B,Stehle P,Fürst P

更新日期：1991-10-01 00:00:00

abstract：:Recently, the development of "green" methods for fabrication of silver nanoparticles (Ag-NPs) has been emphasized, in view of their environmental safety, feasibility, and low cost. In this study, a serine protease, EuP-82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl-NPs) i...

journal_title：Biotechnology and applied biochemistry

authors： Siritapetawee J,Limphirat W,Nantapong N,Songthamwat D

更新日期：2018-07-01 00:00:00

abstract：:Rapid progress has been made in the design and synthesis of oligomers and polymers that emulate the properties of natural proteins. Molecular bioengineering offers the chance to design and produce artificial polymeric proteins with tailored polymeric properties. The elastin-like polypeptides are a well-defined family ...

journal_title：Biotechnology and applied biochemistry

authors： Bandiera A,Taglienti A,Micali F,Pani B,Tamaro M,Crescenzi V,Manzini G

更新日期：2005-12-01 00:00:00

abstract：:Flat panels are the most spread type of photobioreactors for studying light effects on a microalgae culture. Their low thickness, usually between 1 and 3 cm, aims at ensuring light homogeneity across the culture. Yet because optical density has to remain very low, studies are still limited to low cell density cultures...

journal_title：Biotechnology and applied biochemistry

authors： Jiang W,Levasseur W,Casalinho J,Martin T,Puel F,Perré P,Pozzobon V

更新日期：2020-02-03 00:00:00

abstract：:The L-arginine uptake of resting platelets from type-2 diabetic patients and control subjects was measured and the kinetic parameters defined. The effect of platelet stimulation with agonists was also investigated. Kinetic studies showed that the K(m) value for L-arginine transport was not different in patients compar...

journal_title：Biotechnology and applied biochemistry

authors： Signorello MG,Giovine M,Pascale R,Cataldi L,Bordone C,Benatti U,Leoncini G

更新日期：2001-08-01 00:00:00