Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photoactivation and investigation of their biological activities.

abstract：:A deletion htpR mutant of Escherichia coli has been constructed on the basis of site-directed mutagenesis. To this end, the chromosomal allele of htpR gene was substituted by a mutant allele introduced into the cell with a recombinant plasmid. The htpR mutant is characterized by a reduced level of proteolysis and ther...

journal_title：Biotechnology and applied biochemistry

authors： Kiselev VI

更新日期：1988-10-01 00:00:00

abstract：:Among the heat-shock proteins (HSPs) induced in response to heat-shock (HS) in all organisms, the low-molecular-mass (LMM) HSPs are the most abundant and unique in plants. The HSP genes at the transcriptional and the translational levels have been well-characterized; however, relatively little is known about the physi...

journal_title：Biotechnology and applied biochemistry

authors： Yeh KW,Jinn TL,Yeh CH,Chen YM,Lin CY

更新日期：1994-02-01 00:00:00

abstract：:Entrapped cells and entrapped cells grown inside of a calcium alginate matrix as well as free cells have been investigated using Saccharomyces cerevisiae mutant strains with regard to their pattern of growth and invertase activity. The repression of invertase by glucose and glucose-consumption ability were considered ...

journal_title：Biotechnology and applied biochemistry

authors： Rossi-Alva JC,Rocha-Leão MH

更新日期：2003-08-01 00:00:00

abstract：:The aspartic proteinase gene of Mucor pusillus rennin expressed in Pichia pastoris was characterized in terms of structural and conformational stability induced by temperature. This enzyme is 12% glycosylated, with a similar specific activity to the native fungal enzyme. The secondary structure determined by CD is mai...

journal_title：Biotechnology and applied biochemistry

authors： Beldarraín A,Acosta N,Montesinos R,Mata M,Cremata J

更新日期：2000-04-01 00:00:00

abstract：:A truncated precursor form of prostate-specific antigen (PSA), [-2]proPSA, is a well-known biomarker for prostate cancer. To develop a biomarker assay, highly purified [-2]proPSA is required as a standard reference and for generation of a specific antibody. In this study, we generated an efficient mammalian expression...

journal_title：Biotechnology and applied biochemistry

authors： Hwang D,Yoon A,Kim S,Kim H,Chung J

更新日期：2017-05-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of garlic (Allium sativum L.) on the diabetic nephropathy and oxidative stress induced by STZ (streptozotocin) in rats. Diabetes was induced in Male Sprague-Dawley rats by administering a single intraperitoneal injection of STZ (60 mg/kg of body weight). ...

journal_title：Biotechnology and applied biochemistry

authors： Mariee AD,Abd-Allah GM,El-Yamany MF

更新日期：2009-03-01 00:00:00

abstract：:In our work, one-step electro-deposition method was adopted to produce polyaniline (PANI) and functional multiwalled carbon nanotubes (f-MWCNTs) films on glass carbon electrodes, and the modified electrodes were applied as an electrochemical sensor for determination of 10-hydroxycamptothecine (10-HCPT). The f-MWCNTs w...

journal_title：Biotechnology and applied biochemistry

authors： Meng L,Dai J,Rong S,Gao H,Zhang Z,Zhang Y,Chang D,Pan H

更新日期：2020-09-24 00:00:00

abstract：:2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a ...

journal_title：Biotechnology and applied biochemistry

authors： Lautié E,Fliniaux MA,Villarreal ML

更新日期：2010-03-12 00:00:00

abstract：:Most large-scale microbial production of recombinant proteins are based on Escherichia coli, yeasts, or filamentous fungi systems. Using eukaryotic hosts, antibody fragments are generally expressed by targeting to the secretory pathway. This enables not only efficient disulfide bond formation but also secretion of sol...

journal_title：Biotechnology and applied biochemistry

authors： Ezzine A,M'hirsi El Adab S,Bouhaouala-Zahar B,Hmila I,Baciou L,Marzouki MN

更新日期：2012-01-01 00:00:00

abstract：:In the present study, recombinant HCV (hepatitis C virus) core proteins enhanced the immune response elicited by a co-delivered DNA vaccine encoding HCV core and envelope proteins. A mixture of the plasmid pIDKE2 and Co.173, a protein comprising the first 173 amino acids of HCV core, in particular induces a strong hum...

journal_title：Biotechnology and applied biochemistry

authors： Alvarez-Lajonchere L,González M,Alvarez-Obregón JC,Guerra I,Viña A,Acosta-Rivero N,Musacchio A,Morales J,Dueñas-Carrera S

更新日期：2006-04-01 00:00:00

abstract：:The use of the recombinant BoNT/A-Hc (carboxylic domain of the Clostridium botulinum neurotoxin heavy chain) has been proposed as a vaccine candidate for botulism. This fragment contains the principle protective antigenic determinant. In the present study, in order to maximize recombinant protein expression, after ver...

journal_title：Biotechnology and applied biochemistry

authors： Yari K,Fatemi SS,Tavallaei M

更新日期：2010-05-24 00:00:00

abstract：:Empty fruit bunch (EFB) of oil palm trees was converted to fermentable sugars by the combined use of dilute acids and whole fungal cell culture-catalyzed hydrolyses. EFB (5%, w/v) was hydrolyzed in the presence of 0.5% H2 SO4 and 0.2% H3 PO4 at 160 °C for 10 Min. The solid fraction was separated from the acid hydrolys...

journal_title：Biotechnology and applied biochemistry

authors： Li Q,Ng WT,Puah SM,Bhaskar RV,Soh LS,MacBeath C,Parakattil P,Green P,Wu JC

更新日期：2014-07-01 00:00:00

abstract：:Green fluorescent protein (GFP) is a globular protein used as biosensor and biomarker in medical and industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-d-1-thiogalactopyranoside (IPTG), the number of GFP applications are still scarce. This...

journal_title：Biotechnology and applied biochemistry

authors： Lopes C,Dos Santos NV,Dupont J,Pedrolli DB,Valentini SR,de Carvalho Santos-Ebinuma V,Pereira JFB

更新日期：2019-07-01 00:00:00

abstract：:Glutamate dehydrogenase (GDH) and alcohol dehydrogenase (ADH) have been encapsulated in sheep and human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At a fixed enzyme level in the dialysis bag (100 units/ml of RBCs), a significant encapsulation yield was observed for ADH, both in human ...

journal_title：Biotechnology and applied biochemistry

authors： Sanz S,Pinilla M,Garín M,Tipton KF,Luque J

更新日期：1995-10-01 00:00:00

abstract：:Synthetic membranes containing 10% acrylamide units were subjected to activation with formaldehyde at pH 7.5 and 45 degrees C. Trypsin, invertase, and urease were bound to this activated membrane and the kinetic properties of immobilized enzymes were studied. The permeability of the membrane for distilled water manife...

journal_title：Biotechnology and applied biochemistry

authors： Krysteva MA,Shopova BI,Yotova LY,Karasavova MI

更新日期：1991-02-01 00:00:00

abstract：:We have explored the adaptation of the cytochromes P450 (P450) of deep-sea bacteria to high hydrostatic pressures. Strict conservation of the protein fold and functional importance of protein-bound water make P450 a unique subject for the studies of high-pressure adaptation. Earlier, we expressed and purified a fatty-...

journal_title：Biotechnology and applied biochemistry

authors： Davydov DR,Sineva EV,Davydova NY,Bartlett DH,Halpert JR

更新日期：2013-01-01 00:00:00

abstract：:A microbial fuel cell is a sustainable and environmental-friendly device that combines electricity generation and wastewater treatment through metabolic activities of microorganisms. However, low power output from inadequate electron transfer to the anode electrode hampers its practical implementation. Nanocomposites ...

journal_title：Biotechnology and applied biochemistry

authors： Sirajudeen AAO,Annuar MSM,Subramaniam R

更新日期：2020-04-21 00:00:00

abstract：:Monoclonal antibodies specific to human prolactin were generated by an improved hybridoma technique. Following immunization with hormone conjugated with hemocyanin and cell fusion, hybrid cells were first cultured in a semisolid medium containing methylcellulose and later transferred to liquid medium for subculture. O...

journal_title：Biotechnology and applied biochemistry

authors： Lee CY,Chow SN,Coleman P,DeTuri MP

更新日期：1987-02-01 00:00:00

abstract：:The interpretation of the circular dichroism (c.d.) spectra of proteins to date requires additional secondary structural information of the proteins to be analysed (e.g., X-ray or n.m.r. data). Therefore these methods are inappropriate for a c.d. database whose secondary structures are unknown, as in the case of the m...

journal_title：Biotechnology and applied biochemistry

authors： Fasman GD

更新日期：1993-10-01 00:00:00

abstract：:The protective antigen (PA) component of anthrax toxin mediates delivery of either lethal factor (LF) or oedema factor into the cytosol of mammalian cells. The N-terminal domain of LF(1-254) (amino acids 1-254 of LF) binds to PA and, when fused to heterologous proteins, delivers such proteins into the cytosol. In the ...

journal_title：Biotechnology and applied biochemistry

authors： Sharma M,Khanna H,Arora N,Singh Y

更新日期：2000-08-01 00:00:00

abstract：:Mirabilis himalaica is an endangered medicinal plant species in the Tibetan Plateau. The two genes respectively encoding chalcone synthase (MhCHS) and chalcone isomerase (MhCHI) were isolated and characterized from M. himalaica. The sequence analysis revealed that the two genes were similar with their corresponding ho...

journal_title：Biotechnology and applied biochemistry

authors： Lan X,Quan H,Xia X,Yin W,Zheng W

更新日期：2016-05-01 00:00:00

abstract：:Single base mismatched oligonucleotides related to the rpoB gene of Mycobacterium tuberculosis, the mutations of which cause drug resistance of the infectious agent, were detected and discriminated using a surface plasmon resonance biosensor system. Thiol-modified oligonucleotides of the selected sequence (the probe) ...

journal_title：Biotechnology and applied biochemistry

authors： Rachkov A,Patskovsky S,Soldatkin A,Meunier M

更新日期：2013-07-01 00:00:00

abstract：:Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcinoembryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in th...

journal_title：Biotechnology and applied biochemistry

authors： Mao Q,Liu X,Chen C,Ye J,Liang H,Li B,Sun X,Sun L,Sun Y,Mu X,Li L

更新日期：2018-11-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is widely used for the production of recombinant proteins for a variety of applications; however, a number of challenges are typically encountered by researchers depending on the properties of the specific proteins in question. Here, we describe technical issues we have...

journal_title：Biotechnology and applied biochemistry

authors： Belak ZR,Nair M,Ovsenek N

更新日期：2011-05-01 00:00:00

abstract：:The pH optimum, temperature-dependence, thermal stability, substrate specificity and subsite affinities of the 66 kDa, pI 4.0 glucoamylase of the filamentous fungus Trichoderma reesei were determined. It had a pH optimum of 5.5 and a temperature optimum (5 min reaction time) of 70 degrees C with soluble starch as subs...

journal_title：Biotechnology and applied biochemistry

authors： Fagerström R,Kalkkinen N

更新日期：1995-04-01 00:00:00

abstract：:The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With ...

journal_title：Biotechnology and applied biochemistry

authors： Hou KC,Zaniewski R

更新日期：1990-06-01 00:00:00

abstract：:The present study elaborates a cost-effective and transfectant-free method for generating recombinant Bombyx mori (silkworm) nucleopolyhedrovirus in silkworm larvae and pupae by injecting invasive Escherichia coli carrying BmBacmid [BmNPV (B. mori nucleopolyhedrovirus)-Bacmid] into larval haemocoel. Up to 109 PFU (pla...

journal_title：Biotechnology and applied biochemistry

authors： Sun J,Yao L,Yao N,Xu H,Jin P,Kan Y

更新日期：2010-12-01 00:00:00

abstract：:Mustard tuber wastewater is characterized by high salinity and high organic content that is potentially detrimental to the biological treatment system and affects the treatment efficiency accordingly. The experiment used the integrated bioreactor to reduce much of the organics in mustard tuber wastewater, and found th...

journal_title：Biotechnology and applied biochemistry

authors： Kang W,Chai H,Yang S,Du G,Zhou J,He Q

更新日期：2016-07-01 00:00:00

abstract：:We studied, for the first time, characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of enzyme conformational stability and structural behaviour induced by temperature as a function of pH using enzymic assays, differential scanning calorimetry, fluo...

journal_title：Biotechnology and applied biochemistry

authors： Acosta N,Beldarraín A,Rodríguez L,Alonso Y

更新日期：2000-12-01 00:00:00

abstract：:Glutaraldehyde crosslinked, immunoprecipitated gulonolactone oxidase, injected intraperitoneally, has significant catalytic activity and is capable of providing long-term therapeutic benefit for the enzyme deficiency disease scurvy. The enzyme is tolerated even in repetitive doses. In the present study, however, we ha...

journal_title：Biotechnology and applied biochemistry

authors： Sato P,Lindemann D

更新日期：1986-04-01 00:00:00