Molecular cloning and transgenic characterization of the genes encoding chalcone synthase and chalcone isomerase from the Tibetan herbal plant Mirabilis himalaica.

abstract：:A kinetic model was developed to describe recombinant human protein disulphide isomerase (rhPDI)-assisted folding of a substrate protein, C125A recombinant human interleukin-2 (C125A rhIL-2). A series of progress curves showing native C125A rhIL-2 formation under different reaction conditions were generated. Non-linea...

journal_title：Biotechnology and applied biochemistry

authors： Du C,Wolfe JL

更新日期：2003-04-01 00:00:00

abstract：:High yields of extracellular alpha-galactosidase from fungal cultures were obtained by inducing enzyme production with guar gum (a galactomannan obtained from the seeds of Cyamopsis tetragonobola) as the sole carbon source. An alpha-galactosidase was isolated from the culture medium of Penicillium ochrochloron culture...

journal_title：Biotechnology and applied biochemistry

authors： Dey PM,Patel S,Brownleader MD

更新日期：1993-06-01 00:00:00

abstract：:The dynamic gassing-out method was used to assess the effect of stirrer speed, air flow rate and alginate concentration on the overall volumetric oxygen mass transfer coefficient (K(L)a) in a bench-top stirred fermenter equipped with two Rushton-type turbines. As the alginate concentration in the medium was increased ...

journal_title：Biotechnology and applied biochemistry

authors： Parente E,Piraino P,Fidaleo M,Moresi M

更新日期：2004-10-01 00:00:00

abstract：:Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for bo...

journal_title：Biotechnology and applied biochemistry

authors： Parascandola P,de Alteriis E

更新日期：1996-02-01 00:00:00

abstract：:Single base mismatched oligonucleotides related to the rpoB gene of Mycobacterium tuberculosis, the mutations of which cause drug resistance of the infectious agent, were detected and discriminated using a surface plasmon resonance biosensor system. Thiol-modified oligonucleotides of the selected sequence (the probe) ...

journal_title：Biotechnology and applied biochemistry

authors： Rachkov A,Patskovsky S,Soldatkin A,Meunier M

更新日期：2013-07-01 00:00:00

abstract：:Medulloblastoma is the most common malignant pediatric brain tumor in children. GPR137 is a ubiquitously expressed gene in the central nervous system. It has been reported that GPR137 modulates malignant proliferation of glioma cells. However, the relationship between GPR137 and medulloblastoma is still unknown. In th...

journal_title：Biotechnology and applied biochemistry

authors： Wang C,Liang Q,Chen G,Jing J,Wang S

更新日期：2015-11-01 00:00:00

abstract：:The main metabolic properties of human red blood cells (RBC) overloaded with glucose catabolizing enzymes such as hexokinase and glucose oxidase were evaluated. Human erythrocytes loaded with human hexokinase metabolized 3.1 +/- 0.2 mumol/h/ml RBC of glucose, an amount double that consumed by normal and unloaded cells...

journal_title：Biotechnology and applied biochemistry

authors： Rossi L,Bianchi M,Magnani M

更新日期：1992-04-01 00:00:00

abstract：:Recently, hPNAS-4 [human PNAS-4; also known as C1orf121 (Genbank accession number NP-057160)] was reported to be a novel pro-apoptotic protein in mammalian cells. However, at present there is little information about hPNAS-4 and its molecular mechanisms. In our present studies, the hPNAS-4 gene was first cloned into t...

journal_title：Biotechnology and applied biochemistry

authors： Deng H,Jiang Q,Liang S,Yan F,Hou S,Qian Z,Li J,Wen Y,Yang J,Wei Y

更新日期：2010-02-11 00:00:00

abstract：:A microbial fuel cell is a sustainable and environmental-friendly device that combines electricity generation and wastewater treatment through metabolic activities of microorganisms. However, low power output from inadequate electron transfer to the anode electrode hampers its practical implementation. Nanocomposites ...

journal_title：Biotechnology and applied biochemistry

authors： Sirajudeen AAO,Annuar MSM,Subramaniam R

更新日期：2020-04-21 00:00:00

abstract：:Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to...

journal_title：Biotechnology and applied biochemistry

authors： Guzmán-Rodríguez F,Alatorre-Santamaría S,Gómez-Ruiz L,Rodríguez-Serrano G,García-Garibay M,Cruz-Guerrero A

更新日期：2019-03-01 00:00:00

abstract：:The human placenta is a rich raw material for production of many biopharmaceutical products. Here we describe a co-purification process for the production of four different proteins from haemolysed human placenta blood: IgG, catalase (Cat), superoxide dismutase (Sod) and albumin (Alb). The process can be divided in tw...

journal_title：Biotechnology and applied biochemistry

authors： Grellet S,Martins EA,Maimoni Gonçalves V,Yague Lopes AP,Raw I,Cabrera-Crespo J

更新日期：2001-12-01 00:00:00

abstract：:Nicotine is a potent parasympathomimetic stimulant and an important natural alkaloid mainly found in the Nicotiana genus of plants. It can directly threaten ecological security and human health in tobacco waste, wastewater, and other forms of tobacco production. Therefore, it is the basis of nicotine pollution prevent...

journal_title：Biotechnology and applied biochemistry

authors： Ruan A,Gao Y,Fang C,Xu Y

更新日期：2018-11-01 00:00:00

abstract：:As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...

journal_title：Biotechnology and applied biochemistry

authors： Hasegawa M,Kaku T,Kuroda M,Ise N,Kitano H

更新日期：1992-02-01 00:00:00

abstract：:2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a ...

journal_title：Biotechnology and applied biochemistry

authors： Lautié E,Fliniaux MA,Villarreal ML

更新日期：2010-03-12 00:00:00

abstract：:The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as...

journal_title：Biotechnology and applied biochemistry

authors： Cannio R,de Pascale D,Rossi M,Bartolucci S

更新日期：1994-04-01 00:00:00

abstract：:The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently, we reported that a de novo carbene transferase, a tetra-α-helical c-type heme-containing protein, C45, is proficient at N-H insertion reactions, proceeding via the intermolecular transfer of a metallocarben...

journal_title：Biotechnology and applied biochemistry

authors： Stenner R,Anderson JLR

更新日期：2020-07-01 00:00:00

abstract：:Future developments in gene therapy and DNA vaccination depend on cost-effective large-scale production of pharmaceutical-grade pDNA (plasmid DNA). Given the large amount of impurities present in the feedstock, purification processes that have high specificity and capacity at a moderate cost are required. In the prese...

journal_title：Biotechnology and applied biochemistry

authors： Frerix A,Müller M,Kula MR,Hubbuch J

更新日期：2005-08-01 00:00:00

abstract：:hES (human embryonic stem) cells hold tremendous potential in the newly emerging field of regenerative medicine, in addition to being a useful tool in basic scientific research and for pharmacological and cytotoxicity screening. However, an essential prerequisite for the future widespread application of hES cells are ...

journal_title：Biotechnology and applied biochemistry

authors： Heng BC,Kuleshova LL,Bested SM,Liu H,Cao T

更新日期：2005-04-01 00:00:00

abstract：:An extremely thermostable UDP-GlcNAc pyrophosphorylase has been purified from Thermus caldophilus GK24 by chromatographic methods including ion-exchange, hydrophobic interaction, and affinity chromatographies. The specific activity of the enzyme was enriched 41.8-fold, with a recovery of 2%. The molecular mass of the ...

journal_title：Biotechnology and applied biochemistry

authors： Kim JS,Koh S,Shin HJ,Lee DS,Lee SY

更新日期：1999-02-01 00:00:00

abstract：:The structural underpinnings of O₂ activation in P450s have been limited by the inability to trap unstable oxy complexes in the crystalline state for structure determination. To date there are only two known oxy P450 structures. Even so, much is known about O₂ activation in P450cam and the role that redox partner bind...

journal_title：Biotechnology and applied biochemistry

authors： Poulos TL,Madrona Y

更新日期：2013-01-01 00:00:00

abstract：:A deletion htpR mutant of Escherichia coli has been constructed on the basis of site-directed mutagenesis. To this end, the chromosomal allele of htpR gene was substituted by a mutant allele introduced into the cell with a recombinant plasmid. The htpR mutant is characterized by a reduced level of proteolysis and ther...

journal_title：Biotechnology and applied biochemistry

authors： Kiselev VI

更新日期：1988-10-01 00:00:00

abstract：:Sometimes it is necessary to obtain thrombin from limited amounts of human plasma for laboratory assay. None of the available purification methods easily deals with this subject. The procedure described in the present paper uses a readily available pharmaceutical agent, argatroban, to construct an affinity matrix. Arg...

journal_title：Biotechnology and applied biochemistry

authors： Lefkowitz JB

更新日期：2005-10-01 00:00:00

abstract：:A simple and cost-effective method is described to sequence double-stranded DNA (< 100 ng) using a very small amount of fluorescent sequencing mix (one-tenth of manufacturer-recommended amount). The modification has significant advantages over conventional protocols and can be used to sequence, using a single sequenci...

journal_title：Biotechnology and applied biochemistry

authors： Beg OU,Holt RG

更新日期：1997-08-01 00:00:00

abstract：:Acetone-butanol-ethanol (ABE) fermentation was performed with sugarcane bagasse (SCB) hydrolysate using Clostridium beijerinckii strains. A cost-effective SCB medium was developed with no enzymatic hydrolysis and no supplementation of extra carbon source or expensive nitrogen source. One of the C. beijerinckii strains...

journal_title：Biotechnology and applied biochemistry

authors： Narayanasamy S,Chan KL,Cai H,Abdul Razak AHB,Tay BK,Miao H

更新日期：2020-09-01 00:00:00

abstract：:Higher thermostability or acid resistance for fungal α-amylase will help to improve the sugar-making process and cut down the production costs. Here, the thermostability or acid resistance of Rhizopus oryzae α-amylase (ROAmy) was significantly enhanced by site-directed evolution based on multiple sequence alignment (M...

journal_title：Biotechnology and applied biochemistry

authors： Li S,Yang Q,Tang B

更新日期：2020-07-01 00:00:00

abstract：:We report a novel surface plasmon resonance (SPR) biosensor that uses the full-length Det7 phage tail protein (Det7T) to rapidly and selectively detect Salmonella enterica serovar Typhimurium (S. Typhimurium). Det7T, which was obtained using recombinant protein expression and purification in Escherichia coli, demonstr...

journal_title：Biotechnology and applied biochemistry

authors： Hyeon SH,Lim WK,Shin HJ

更新日期：2020-01-08 00:00:00

abstract：:Yeast cytochrome c peroxidase (CcP) was purified from baker's yeast and immobilised onto a nylon membrane. The kinetics of the soluble and immobilised forms of the enzyme were investigated for the catalysed oxidation of potassium ferrocyanide in the presence of H2O2 and m-chloroperoxybenzoic acid. The pH dependence of...

journal_title：Biotechnology and applied biochemistry

authors： Cooper JM,McNeil CJ,Bannister JV

更新日期：1991-08-01 00:00:00

abstract：:Mouse and human erythrocytes are inherently different with respect to slow dialysis encapsulation used in preparing carrier erythrocytes. Fluorescein isothiocyanate (FITC)-dextran was added to five different stages of the encapsulation process to discern when endocytic vesiculation occurred. Mouse erythrocytes were mu...

journal_title：Biotechnology and applied biochemistry

authors： DeLoach JR,Droleskey RE

更新日期：1993-08-01 00:00:00

abstract：:Cardiolipin, the primary lipid hapten in the antigen suspension used for the detection of antitreponemal antibodies in the sera of syphilitic patients, was successfully coupled to glucose oxidase, peroxidase, and some other enzymes using different crosslinking agents. These complexes were used to replace the pure unco...

journal_title：Biotechnology and applied biochemistry

authors： Ngeh-Ngwainbi J,Kuan SS,Steinman F,Guilbault GG

更新日期：1986-12-01 00:00:00

abstract：:Bioreactor process changes can have a profound effect on the yield and quality of biotechnology products. Mannose-6-phosphate (M6P) glycan content and the enzymatic catalytic kinetic parameters are critical quality attributes (CQAs) of many therapeutic enzymes used to treat lysosomal storage diseases (LSDs). Here, we ...

journal_title：Biotechnology and applied biochemistry

authors： Madhavarao CN,Agarabi CD,Wong L,Müller-Loennies S,Braulke T,Khan M,Anderson H,Johnson GR

更新日期：2014-03-01 00:00:00