The use of plant cell wall-degrading enzymes from newly isolated Penicillium ochrochloron Biourge for viscosity reduction in ethanol production with fresh sweet potato tubers as feedstock.

abstract：:Future developments in gene therapy and DNA vaccination depend on cost-effective large-scale production of pharmaceutical-grade pDNA (plasmid DNA). Given the large amount of impurities present in the feedstock, purification processes that have high specificity and capacity at a moderate cost are required. In the prese...

journal_title：Biotechnology and applied biochemistry

authors： Frerix A,Müller M,Kula MR,Hubbuch J

更新日期：2005-08-01 00:00:00

abstract：:In the present study, the effect of the neuroleptics chlorpromazine (2-chloro-N,N-dimethyl-10H-phenothiazine-10-propanamine) and trifluoperazine {10-[3-(4-methylpiperazin-l-yl)-propyl]-2-trifluoromethyl)-10-H- [phenothiazine}-10H-phenothiazine¿ on the EPR-spectroscopic properties of ferrous human adult nitrosylated ha...

journal_title：Biotechnology and applied biochemistry

authors： Ascenzi P,Bertollini A,Coletta M,Lucacchini A

更新日期：1999-10-01 00:00:00

abstract：:Cordyceps sinensis (Chinese caterpillar fungus) is a fungus parasitic on the larvae of Lepidoptera and has been considered to be a precious tonic food and herbal medicine since ancient times in China. Recently, some fungal strains have been isolated from the fruiting bodies of wild C. sinensis, and some of them have b...

journal_title：Biotechnology and applied biochemistry

authors： Zhang W,Yang J,Chen J,Hou Y,Han X

更新日期：2005-08-01 00:00:00

abstract：:We have analysed the status and transcriptional activity of the bovine papillomavirus-I (complete BPV-I genome)-based expression vector pCES in CI27i-cell-line-derived 3TI cells used for the industrial production of recombinant human erythropoietin (rhuEpo). Complete tandem head-to-tail integration of about 600 vector...

journal_title：Biotechnology and applied biochemistry

authors： Fibi MR,Kunz J,Weimer T,Schulz R,Teifke P,Grzeschik KH,Johannsen R,Zettlmeissl G

更新日期：1996-02-01 00:00:00

abstract：:Naproxen and bezafibrate fall into the category of pharmaceuticals that have been widely detected in the aquatic environment, and one of the major sources is the effluent discharge from wastewater treatment plants. This study investigated the sorption and degradation kinetics of naproxen and bezafibrate in the presenc...

journal_title：Biotechnology and applied biochemistry

authors： Tang Y,Li XM,Xu ZC,Guo QW,Hong CY,Bing YX

更新日期：2014-05-01 00:00:00

abstract：:Glycoprotein D (gD2) is the most important candidate antigen for herpes simplex virus type 2 (HSV-2) vaccine development. Establishment of a stable eukaryotic cell line to overexpress gD2 and an efficient purification process to purify is essential for the development of subunit vaccine against HSV-2. The DNA sequence...

journal_title：Biotechnology and applied biochemistry

authors： Qi Y,Xu Y,Pan Y,Li S,Li B,Pan M,Zhang S,Li Y

更新日期：2016-05-01 00:00:00

abstract：:Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to...

journal_title：Biotechnology and applied biochemistry

authors： Guzmán-Rodríguez F,Alatorre-Santamaría S,Gómez-Ruiz L,Rodríguez-Serrano G,García-Garibay M,Cruz-Guerrero A

更新日期：2019-03-01 00:00:00

abstract：:Succinylcholine apnea happens in cases of null butyrylcholinesterase activity after administration of preintubation succinylcholine. So far, there is no such popular test that can rapidly screen null butyrylcholinesterase activity from plasma. Development of a novel method for rapid screening of null butyrylcholineste...

journal_title：Biotechnology and applied biochemistry

authors： Nagpal N,Chowdhary S,Bhattacharyya R,Banerjee D

更新日期：2015-03-01 00:00:00

abstract：:The L-arginine uptake of resting platelets from type-2 diabetic patients and control subjects was measured and the kinetic parameters defined. The effect of platelet stimulation with agonists was also investigated. Kinetic studies showed that the K(m) value for L-arginine transport was not different in patients compar...

journal_title：Biotechnology and applied biochemistry

authors： Signorello MG,Giovine M,Pascale R,Cataldi L,Bordone C,Benatti U,Leoncini G

更新日期：2001-08-01 00:00:00

abstract：:The structural gene of elastase strain K (elastase from Pseudomonas aeruginosa strain K), namely HindIII1500PstI, was successfully sequenced to contain 1497 bp. The amino acid sequence, deduced from the nucleotide sequence, revealed that the mature elastase consists of 301 amino acids, with a molecular mass of 33.1 kD...

journal_title：Biotechnology and applied biochemistry

authors： Wong CF,Salleh AB,Basri M,Abd Rahman RN

更新日期：2010-09-01 00:00:00

abstract：:Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcinoembryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in th...

journal_title：Biotechnology and applied biochemistry

authors： Mao Q,Liu X,Chen C,Ye J,Liang H,Li B,Sun X,Sun L,Sun Y,Mu X,Li L

更新日期：2018-11-01 00:00:00

abstract：:Recently, hPNAS-4 [human PNAS-4; also known as C1orf121 (Genbank accession number NP-057160)] was reported to be a novel pro-apoptotic protein in mammalian cells. However, at present there is little information about hPNAS-4 and its molecular mechanisms. In our present studies, the hPNAS-4 gene was first cloned into t...

journal_title：Biotechnology and applied biochemistry

authors： Deng H,Jiang Q,Liang S,Yan F,Hou S,Qian Z,Li J,Wen Y,Yang J,Wei Y

更新日期：2010-02-11 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) still represents a major problem to the swine industry worldwide, causing high mortality rates in infected animals. Virus-like particles (VLPs) have gained attention for vaccine development, serving both as scaffolds for epitope expression and immune response enhancers. The commercial ...

journal_title：Biotechnology and applied biochemistry

authors： Aguilera BE,Chávez-Calvillo G,Elizondo-Quiroga D,Jimenez-García MN,Carrillo-Tripp M,Silva-Rosales L,Hernández-Gutiérrez R,Gutiérrez-Ortega A

更新日期：2017-05-01 00:00:00

abstract：:The immobilization of F(0)F(1)-ATPase in uniform orientation is reported. The biotinylated and histidine-tagged subunits of the bacterial F(0)F(1)-ATPase complex were used for immobilization of the complex on artificial semi-permeable membranes resulting in 88+/-7.8 and 72+/-5.2% coupling of the enzymes. The immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2004-06-01 00:00:00

abstract：:This paper is concerned with the optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Auricularia polytricha by one-factor-at-a-time and uniform design (UD) methods. First, the one-factor-at-a-time method was adopted to investigate the effects of environmental factors (i.e....

journal_title：Biotechnology and applied biochemistry

authors： Xu CP,Yun JW

更新日期：2003-10-01 00:00:00

abstract：:The conformation of a protein refers to the three-dimensional arrangement of its constituent atoms. Since expression of the biological activity of a protein depends on its conformation, it is clear that full characterization of a protein involves an understanding of its three-dimensional structure. This review outline...

journal_title：Biotechnology and applied biochemistry

authors： Price NC

更新日期：2000-02-01 00:00:00

abstract：:alpha-Cyclodextrin (CD) was converted by Bacillus macerans cyclodextrin glucanotransferase into highly insoluble high-amylose starch with a low average degree of polymerization (56-73), in yields as high as 78% over a wide range of temperatures (25-70 degreesC). Ability to undergo this conversion was highly concentrat...

journal_title：Biotechnology and applied biochemistry

authors： Rendleman JA Jr,Knutson CA Jr

更新日期：1998-12-01 00:00:00

abstract：:As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, there is currently a lack of simple and effective method for quantitative detection...

journal_title：Biotechnology and applied biochemistry

authors： Chen C,Liang H,Hu B,Ning B,Lai H,He Y,Guo G,Zhong S,Li L

更新日期：2020-06-13 00:00:00

abstract：:A three-layered simulation methodology is described that rapidly evaluates biomanufacturing process options. In each layer, inferior options are screened out, while more promising candidates are evaluated further in the subsequent, more refined layer, which uses more rigorous models that require more data from time-co...

journal_title：Biotechnology and applied biochemistry

authors： Chhatre S,Francis R,O'Donovan K,Titchener-Hooker NJ,Newcombe AR,Keshavarz-Moore E

更新日期：2007-10-01 00:00:00

abstract：:Most large-scale microbial production of recombinant proteins are based on Escherichia coli, yeasts, or filamentous fungi systems. Using eukaryotic hosts, antibody fragments are generally expressed by targeting to the secretory pathway. This enables not only efficient disulfide bond formation but also secretion of sol...

journal_title：Biotechnology and applied biochemistry

authors： Ezzine A,M'hirsi El Adab S,Bouhaouala-Zahar B,Hmila I,Baciou L,Marzouki MN

更新日期：2012-01-01 00:00:00

abstract：:The development of lytic bacteriophages as therapeutic products is an attractive alternative to antibiotics. In this study, we evaluated the potential of phage tails for lysing Gram-positive bacteria. Phage P954, a well-characterized temperate staphylococcal phage, was found to adsorb to a large number of Staphylococc...

journal_title：Biotechnology and applied biochemistry

authors： Biddappa AC,Sundarrajan S,Ramalinga AB,Sriram B,Padmanabhan S

更新日期：2012-11-01 00:00:00

abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...

journal_title：Biotechnology and applied biochemistry

authors： Hiragami F,Akiyama J,Koike Y,Kano Y

更新日期：2007-05-01 00:00:00

abstract：:Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (E...

journal_title：Biotechnology and applied biochemistry

authors： Lau MF,Chua KH,Sabaratnam V,Kuppusamy UR

更新日期：2020-08-28 00:00:00

abstract：:In order to impart an ability for receptor-independent transmembrane transfer to water-soluble proteins, it has been suggested that they be hydrophobized by lipid groups (fatty acids, etc.). To this end, systems of reversed micelles of surfactants in organic solvents were used as reaction media for protein modificatio...

journal_title：Biotechnology and applied biochemistry

authors： Alakhov VYu,Kabanov AV,Batrakova EV,Koromyslova IA,Levashov AV,Severin ES

更新日期：1990-02-01 00:00:00

abstract：:Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an au...

journal_title：Biotechnology and applied biochemistry

authors： Jantra J,Zór K,Sanders M,De Saeger S,Hedström M,Mattiasson B

更新日期：2020-05-01 00:00:00

abstract：:Medulloblastoma is the most common malignant pediatric brain tumor in children. GPR137 is a ubiquitously expressed gene in the central nervous system. It has been reported that GPR137 modulates malignant proliferation of glioma cells. However, the relationship between GPR137 and medulloblastoma is still unknown. In th...

journal_title：Biotechnology and applied biochemistry

authors： Wang C,Liang Q,Chen G,Jing J,Wang S

更新日期：2015-11-01 00:00:00

abstract：:Two monoclonal antibodies against human myeloperoxidase, designated 3-2H3 and 4-2C11, were produced and characterized. Both bound to the native enzyme, but neither bound to the denatured enzyme or to its two denatured subunits. 4-2C11 bound to the three types of leukocyte myeloperoxidase, I, II, and III, as well as to...

journal_title：Biotechnology and applied biochemistry

authors： Hur SJ,Yamada M

更新日期：1986-12-01 00:00:00

abstract：:This review article focuses on the use of the methylotrophic yeast Pichia pastoris as a recombinant protein-expression system. P. pastoris is a useful system for the expression of milligram-to-gram quantities of a protein, which can be scaled up to fermentation to meet greater demands. Compared with mammalian cells, P...

journal_title：Biotechnology and applied biochemistry

authors： Fischer R,Drossard J,Emans N,Commandeur U,Hellwig S

更新日期：1999-10-01 00:00:00

abstract：:The variation with pH of the kinetic parameters of penicillin acylase from Kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. The pH-dependence of log (V/Km) for penicillin G showed that a group with a pK value over 4.7 must be deprotonated and t...

journal_title：Biotechnology and applied biochemistry

authors： Martín J,Prieto I,Mancheño JM,Barbero JL,Arche R

更新日期：1993-06-01 00:00:00

abstract：:Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for bo...

journal_title：Biotechnology and applied biochemistry

authors： Parascandola P,de Alteriis E

更新日期：1996-02-01 00:00:00