Development of an automated flow-based spectrophotometric immunoassay for continuous detection of zearalenone.

abstract：:The phosphatase and tensin homolog (PTEN) gene plays a crucial role in signal transduction by negatively regulating the PI3K signaling pathway. It is the most frequent mutated gene in many human-related cancers. Considering its critical role, a functional analysis of missense mutations of PTEN gene was undertaken in t...

journal_title：Biotechnology and applied biochemistry

authors： Khan I,Ansari IA,Singh P,Dass J FP

更新日期：2017-09-01 00:00:00

abstract：:The urinary C-terminal telopeptide fragment of type II collagen (uCTX-II) has been reported as the efficient blood-based biomarker for osteoarthritis, which affects knees, hands, spine, and hips. This study reports a sensing strategy with antibody-conjugated gold nanoparticles (GNP) on an interdigitated electrode (IDE...

journal_title：Biotechnology and applied biochemistry

authors： Wang S,Su S,Yu C,Gopinath SCB,Yang Z

更新日期：2020-07-04 00:00:00

abstract：:Catalytic properties of Escherichia coli polyphosphate kinase (EC 2.7.4.1), a promising enzyme for use in ATP regeneration (Hoffman, et al., 1988, Biotechnol. Appl. Biochem. 10, 107-117), are reported here. E. coli polyphosphate kinase (PPK) is broadly active in the pH range 5.5 to 8.5, having an optimal Vmax at pH 7....

journal_title：Biotechnology and applied biochemistry

authors： Haeusler PA,Dieter L,Rittle KJ,Shepler LS,Paszkowski AL,Moe OA

更新日期：1992-04-01 00:00:00

abstract：:Amyloglucosidase from Rhizopus mould and beta-glucosidase from sweet almond were employed for the preparation of phenolic and vitamin glycosides of vanillin, N-vanillylnonanamide, DL-dopa, dopamine, curcumin, alpha-tocopherol (vitamin E), pyridoxine (vitamin B(6)), ergocalciferol (vitamin D(2)), thiamin (vitamin B(1))...

journal_title：Biotechnology and applied biochemistry

authors： Charles RE,Ponrasu T,Sivakumar R,Divakar S

更新日期：2009-03-01 00:00:00

abstract：:The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatog...

journal_title：Biotechnology and applied biochemistry

authors： Rigi G,Ghaedmohammadi S,Ahmadian G

更新日期：2019-05-01 00:00:00

abstract：:High yields of extracellular alpha-galactosidase from fungal cultures were obtained by inducing enzyme production with guar gum (a galactomannan obtained from the seeds of Cyamopsis tetragonobola) as the sole carbon source. An alpha-galactosidase was isolated from the culture medium of Penicillium ochrochloron culture...

journal_title：Biotechnology and applied biochemistry

authors： Dey PM,Patel S,Brownleader MD

更新日期：1993-06-01 00:00:00

abstract：:A simple and cost-effective method is described to sequence double-stranded DNA (< 100 ng) using a very small amount of fluorescent sequencing mix (one-tenth of manufacturer-recommended amount). The modification has significant advantages over conventional protocols and can be used to sequence, using a single sequenci...

journal_title：Biotechnology and applied biochemistry

authors： Beg OU,Holt RG

更新日期：1997-08-01 00:00:00

abstract：:Interleukin-17F (IL-17F) is an important member of IL-17 cytokine family, which plays important roles in host defense against microbial infections. Streptococcus pneumoniae is a common pathogen associated with several invasive and noninvasive pneumococcal diseases, and mucosal immune response plays crucial roles in de...

journal_title：Biotechnology and applied biochemistry

authors： Chen L,Guo S,Wu L,Hao C,Xu W,Zhang J

更新日期：2015-05-01 00:00:00

abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...

journal_title：Biotechnology and applied biochemistry

authors： Ramström M,Zuberovic A,Grönwall C,Hanrieder J,Bergquist J,Hober S

更新日期：2009-02-01 00:00:00

abstract：:A new system equipped with a computer-controlled multiple activity analyzer has been developed for the efficient purification of multiple enzymes. The system consists of the following units: conventional enzyme fractionation system with a peristaltic pump, liquid chromatographic column, fraction collector, and uv moni...

journal_title：Biotechnology and applied biochemistry

authors： Tokushige M,Sasaki S

更新日期：1986-12-01 00:00:00

abstract：:Human thymosin alpha1 is an effective immune system enhancer for the treatment of cancer and viral diseases. Therefore the development of new methods for its synthesis is an urgent problem. In the present work, we propose an efficient scalable scheme for the production of recombinant thymosin alpha1. We used an expres...

journal_title：Biotechnology and applied biochemistry

authors： Esipov RS,Stepanenko VN,Beyrakhova KA,Muravjeva TI,Miroshnikov AI

更新日期：2010-05-19 00:00:00

abstract：:A protein with a molecular mass of 66 kDa was isolated by a simple, rapid, and inexpensive method, using 3-N-morpholinopropanesulfonic acid, potassium thiocyanate, and dithiothreitol, from a mixture of spores, parasporal crystals, and cell debris of Bacillus thuringiensis subsp. kurstaki. The protein was active agains...

journal_title：Biotechnology and applied biochemistry

authors： Venkateswerlu G,Stotzky G

更新日期：1990-06-01 00:00:00

abstract：:Penicillium ochrochloron Biourge, which was isolated from rotten sweet potato, can produce plant cell wall-degrading enzymes (PCWDEs) with high viscosity reducing capability for ethanol production using fresh sweet potato tubers as feedstock. The enzyme preparation was characterized by a broad enzyme spectrum includin...

journal_title：Biotechnology and applied biochemistry

authors： Huang Y,Jin Y,Shen W,Fang Y,Zhang G,Zhao H

更新日期：2014-07-01 00:00:00

abstract：:The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a plant species known for its production of TIAs (terpenoid indole alkaloids), many of which are pharmaceutically important. Ajmalicine and serpentine are prescribed for the treatment of hypertension, whereas the bisindoles vinblastine, vincristine and 3',...

journal_title：Biotechnology and applied biochemistry

authors： Zhou ML,Shao JR,Tang YX

更新日期：2009-04-01 00:00:00

abstract：:c-Jun is a highly oncogenic transcription factor involved in the development of different types of cancer. In the present study we have generated c-Jun-binding-affinity proteins from a phage-displayed library of so-called 'Affibody ligands', developed by combinatorial engineering of a non-immunoglobulin-based scaffold...

journal_title：Biotechnology and applied biochemistry

authors： Lundberg E,Brismar H,Gräslund T

更新日期：2009-01-01 00:00:00

abstract：:Bioreactor process changes can have a profound effect on the yield and quality of biotechnology products. Mannose-6-phosphate (M6P) glycan content and the enzymatic catalytic kinetic parameters are critical quality attributes (CQAs) of many therapeutic enzymes used to treat lysosomal storage diseases (LSDs). Here, we ...

journal_title：Biotechnology and applied biochemistry

authors： Madhavarao CN,Agarabi CD,Wong L,Müller-Loennies S,Braulke T,Khan M,Anderson H,Johnson GR

更新日期：2014-03-01 00:00:00

abstract：:We have created a mouse/human chimaeric antibody by taking antigen-binding fragment (Fab) genes of a mouse antibody-producing hybridoma with specificity for bacterial endotoxin and joining them to human Ig crystallizing-fragment (Fc) genes using recombinant DNA techniques. This chimaeric antibody has been expressed in...

journal_title：Biotechnology and applied biochemistry

authors： Tan W,Lam PH

更新日期：1999-08-01 00:00:00

abstract：:A chitin-degrading Bacillus strain, designated as NCTU2, was screened from soil and identified. An extracellular chitinase was purified to >90% homogeneity from the culture filtrate. The purification involved hydrophobic-interaction and gel-filtration chromatographic separations with a yield of 58%. The purified enzym...

journal_title：Biotechnology and applied biochemistry

authors： Wen CM,Tseng CS,Cheng CY,Li YK

更新日期：2002-06-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is widely used for the production of recombinant proteins for a variety of applications; however, a number of challenges are typically encountered by researchers depending on the properties of the specific proteins in question. Here, we describe technical issues we have...

journal_title：Biotechnology and applied biochemistry

authors： Belak ZR,Nair M,Ovsenek N

更新日期：2011-05-01 00:00:00

abstract：:Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, the molecular cause of the disease is poorly elucidated. In the present study, we primarily found that translationally controlled tumor protein (TCTP) was overexpressed in human OS tissues and cell lines. To investigate t...

journal_title：Biotechnology and applied biochemistry

authors： Shen JH,Qu CB,Chu HK,Cui MY,Wang YL,Sun YX,Song YD,Li G,Shi FJ

更新日期：2016-01-01 00:00:00

abstract：:Production of His-BbST [hexahistidine-tagged BbST (bubaline somatotropin)] by auto-induction in high-density shake-flask cultures coupled with a single-step, on-column purification and refolding strategy is described here. To optimize expression of BbST, different media and expression conditions were tested. The highe...

journal_title：Biotechnology and applied biochemistry

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2007-05-01 00:00:00

abstract：:A pathway for the synthesis of the repeating tetrasaccharide units in gellan gum from Pseudomonas elodea is proposed. The enzymes presumed to be involved in the synthesis of the activated precursors UDP-glucose, TDP-rhamnose, and UDP-glucuronic acid were detected and assayed in crude cell extracts of the gellan-produc...

journal_title：Biotechnology and applied biochemistry

authors： Martins LO,Sá-Correia I

更新日期：1991-12-01 00:00:00

abstract：:The production of rabies virus from baby hamster kidney-Ankara66 (BHK-An(66), HUKUK 99050302) monolayer cells was examinedin a packed-bed reactor containing non-woven polyester fabric (NWPF)discs. At first, growth characteristics of the cells were determined instatic culture. The suspension culture studies were realiz...

journal_title：Biotechnology and applied biochemistry

authors： Gümüşderelioğlu M,Aslankaraoğlu E,Gürhan SI

更新日期：2001-06-01 00:00:00

abstract：:Marine biomass, especially the algal biomass, is currently considered to be one of the most potential candidates for biofuels conversion during the development of biomass utilization. In this study, a diluted sulfuric acid pretreatment method was established for biobutanol from red algal biomass Gelidium amansii using...

journal_title：Biotechnology and applied biochemistry

authors： Hong Y,Chen C,Wu YR

更新日期：2020-09-01 00:00:00

abstract：:Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrog...

journal_title：Biotechnology and applied biochemistry

authors： Chirila TV,Suzuki S,Papolla C

更新日期：2017-11-01 00:00:00

abstract：:The arrival of novel health crisis by a novel member of coronavirus group named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by World Health Organization took the whole world in global emergency by affecting 206 countries. The virus infects 206 countries with 86,839,226 confirmed cases, 61,565,949 r...

journal_title：Biotechnology and applied biochemistry

authors： Shivangi S,Meena LS

更新日期：2021-01-12 00:00:00

abstract：:Parkinson's disease (PD) is the most common neurodegenerative movement disorder and is characterized by the loss of dopaminergic neurons and the formation of eosinophilic intracytoplasmic inclusion bodies known as Lewy bodies. Although alpha-synuclein is known to be a pivotal factor implicated in the pathogenesis of P...

journal_title：Biotechnology and applied biochemistry

authors： Choi JY,Sung YM,Park HJ,Hur EH,Lee SJ,Hahn C,Min BR,Kim IK,Kang S,Rhim H

更新日期：2002-08-01 00:00:00

abstract：:Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin-streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplification is overamplific...

journal_title：Biotechnology and applied biochemistry

authors： Shao K,Shi X,Zhu X,Cui L,Shao Q,Ma D

更新日期：2017-03-01 00:00:00

abstract：:It is now a well-known fact that alpha-CD (alpha-cyclodextrin) and Ca(2+) are two important folding agents in the re-activation and refolding processes of many denatured enzyme solutions. Bacillus sp. alpha-amylase was extensively denatured in a 6 M guanidinium chloride solution overnight. Under suitable renaturation ...

journal_title：Biotechnology and applied biochemistry

authors： Yazdanparast R,Khodarahmi R

更新日期：2005-04-01 00:00:00

abstract：:In the erythrocyte membrane, the mobility of band 3 protein, the receptor for concanavalin A (Con A), is drastically reduced by the membrane skeleton. Yet, the vesicles free of membrane skeletal proteins, isolated from the highly agglutinable proteinase-treated cells, are found to be devoid of Con A agglutinability. T...

journal_title：Biotechnology and applied biochemistry

authors： Pestonjamasp KN,Gokhale SM,Mehta NG

更新日期：1990-10-01 00:00:00