An efficient cost-effective protocol for automated fluorescent-DNA sequencing.

abstract：:The immobilization of F(0)F(1)-ATPase in uniform orientation is reported. The biotinylated and histidine-tagged subunits of the bacterial F(0)F(1)-ATPase complex were used for immobilization of the complex on artificial semi-permeable membranes resulting in 88+/-7.8 and 72+/-5.2% coupling of the enzymes. The immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2004-06-01 00:00:00

abstract：:Limit of detection (LOD), limit of quantification, and the dynamic range of detection of hepatitis B surface antigen antibody (anti-HBs) using a surface plasmon resonance (SPR) chip-based approach with Pichia pastoris-derived recombinant hepatitis B surface antigen (HBsAg) as recognition element were established throu...

journal_title：Biotechnology and applied biochemistry

authors： Tam YJ,Zeenathul NA,Rezaei MA,Mustafa NH,Azmi MLM,Bahaman AR,Lo SC,Tan JS,Hani H,Rasedee A

更新日期：2017-09-01 00:00:00

abstract：:Human urokinase was immobilized on an ethylene vinyl acetate copolymer surface. Soluble urokinase showed its maximum activity at pH 8.5, while the immobilized enzyme was most active at pH 9.0. Apparently, the shift in optimal pH was due to the polyanionic nature of the carrier surface on which the enzyme was immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Yabushita Y

更新日期：1988-06-01 00:00:00

abstract：:2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a ...

journal_title：Biotechnology and applied biochemistry

authors： Lautié E,Fliniaux MA,Villarreal ML

更新日期：2010-03-12 00:00:00

abstract：:A protein with a molecular mass of 66 kDa was isolated by a simple, rapid, and inexpensive method, using 3-N-morpholinopropanesulfonic acid, potassium thiocyanate, and dithiothreitol, from a mixture of spores, parasporal crystals, and cell debris of Bacillus thuringiensis subsp. kurstaki. The protein was active agains...

journal_title：Biotechnology and applied biochemistry

authors： Venkateswerlu G,Stotzky G

更新日期：1990-06-01 00:00:00

abstract：:A microbial fuel cell is a sustainable and environmental-friendly device that combines electricity generation and wastewater treatment through metabolic activities of microorganisms. However, low power output from inadequate electron transfer to the anode electrode hampers its practical implementation. Nanocomposites ...

journal_title：Biotechnology and applied biochemistry

authors： Sirajudeen AAO,Annuar MSM,Subramaniam R

更新日期：2020-04-21 00:00:00

abstract：:The extensive literature on natural antimicrobial systems in animals, plants, and microorganisms is surveyed and particular systems are discussed, viz., the peroxidase systems in saliva and milk, singlet oxygen in the phagosome, cecropins and attacins in insects, complement, lysozyme and, to a limited extent, phytoale...

journal_title：Biotechnology and applied biochemistry

authors： Banks JG,Board RG,Sparks NH

更新日期：1986-04-01 00:00:00

abstract：:Auricularia polytricha belonging to Basidiomycota has the ability to degrade lignocellulose. However, there has been no resource in public databases examining the transcriptome of A. polytricha. In this study, high-throughput sequencing platform BGISEQ-500 was used to generate large amount of transcript sequences from...

journal_title：Biotechnology and applied biochemistry

authors： Jiang S,Zheng X,Li L

更新日期：2020-08-12 00:00:00

abstract：:Green fluorescent protein (GFP) is a globular protein used as biosensor and biomarker in medical and industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-d-1-thiogalactopyranoside (IPTG), the number of GFP applications are still scarce. This...

journal_title：Biotechnology and applied biochemistry

authors： Lopes C,Dos Santos NV,Dupont J,Pedrolli DB,Valentini SR,de Carvalho Santos-Ebinuma V,Pereira JFB

更新日期：2019-07-01 00:00:00

abstract：:Neuroblastoma (NB) is an embryonic solid tumor derived from precursor cells of the sympathetic nervous system, and accounts for 11% of childhood cancers and around 15% of cancer deaths in children. SUMOylation and deSUMOylation are dynamic mechanisms regulating a spectrum of protein activities. The SUMO proteases (SEN...

journal_title：Biotechnology and applied biochemistry

authors： Xiang-Ming Y,Zhi-Qiang X,Ting Z,Jian W,Jian P,Li-Qun Y,Ming-Cui F,Hong-Liang X,Xu C,Yun Z

更新日期：2016-05-01 00:00:00

abstract：:Sometimes it is necessary to obtain thrombin from limited amounts of human plasma for laboratory assay. None of the available purification methods easily deals with this subject. The procedure described in the present paper uses a readily available pharmaceutical agent, argatroban, to construct an affinity matrix. Arg...

journal_title：Biotechnology and applied biochemistry

authors： Lefkowitz JB

更新日期：2005-10-01 00:00:00

abstract：:Bioreactor process changes can have a profound effect on the yield and quality of biotechnology products. Mannose-6-phosphate (M6P) glycan content and the enzymatic catalytic kinetic parameters are critical quality attributes (CQAs) of many therapeutic enzymes used to treat lysosomal storage diseases (LSDs). Here, we ...

journal_title：Biotechnology and applied biochemistry

authors： Madhavarao CN,Agarabi CD,Wong L,Müller-Loennies S,Braulke T,Khan M,Anderson H,Johnson GR

更新日期：2014-03-01 00:00:00

abstract：:The phosphatase and tensin homolog (PTEN) gene plays a crucial role in signal transduction by negatively regulating the PI3K signaling pathway. It is the most frequent mutated gene in many human-related cancers. Considering its critical role, a functional analysis of missense mutations of PTEN gene was undertaken in t...

journal_title：Biotechnology and applied biochemistry

authors： Khan I,Ansari IA,Singh P,Dass J FP

更新日期：2017-09-01 00:00:00

abstract：:In the present study, the growth and key metabolic features of a gene-transfected Drosophila melanogaster (fruitfly) S2 (Schneider 2) cell population (S2AcRVGP cells), cultured in Sf900-II medium, have been evaluated to provide substantial support for the development of a bioprocess to produce RVGP (rabies-virus glyco...

journal_title：Biotechnology and applied biochemistry

authors： Swiech K,da Silva CS,Arantes MK,dos Santos AS,Astray RM,Pereira CA,Suazo CA

更新日期：2008-01-01 00:00:00

abstract：:A deletion htpR mutant of Escherichia coli has been constructed on the basis of site-directed mutagenesis. To this end, the chromosomal allele of htpR gene was substituted by a mutant allele introduced into the cell with a recombinant plasmid. The htpR mutant is characterized by a reduced level of proteolysis and ther...

journal_title：Biotechnology and applied biochemistry

authors： Kiselev VI

更新日期：1988-10-01 00:00:00

abstract：:Two different enzymes that metabolize lactate in the presence of oxygen, either to acetate plus CO2 (lactate 2-mono-oxygenase; Lmox) or to pyruvate plus H2O2 (lactate oxidase; Lox) were encapsulated in human and murine red blood cells (RBCs). Lmox shows a low affinity for lactate (Km 22 mM) and thus works at a low rat...

journal_title：Biotechnology and applied biochemistry

authors： Garin M,Rossi L,Luque J,Magnani M

更新日期：1995-12-01 00:00:00

abstract：:Mechano-growth factor (MGF), an alternative splicing variant of insulin-like growth factor-1 (IGF-1) gene, promotes cell proliferation and inhibits cell differentiation. It also plays an important role in tumor development. It is important to optimize the production process and achieve MGF protein because there is no ...

journal_title：Biotechnology and applied biochemistry

authors： Feng J,Wan R,Yi Q,He L,Yang L,Tang L

更新日期：2015-09-01 00:00:00

abstract：:St. John's wort ( Hypericum perforatum L.) is a herbaceous perennial distributed throughout the World that has been widely used in traditional medicine. H. perforatum produces several types of biologically active compound, including the hypericins--a family of light-activated anthraquinones, localized within specializ...

journal_title：Biotechnology and applied biochemistry

authors： Kirakosyan A,Sirvent TM,Gibson DM,Kaufman PB

更新日期：2004-02-01 00:00:00

abstract：:Aspergillus niger and Rhizopus sp. glucoamylases were purified on an affinity chromatography column from commercially available, impure enzyme preparations. Up to 2 mg of glucoamylase protein was bound without leakage to a 1-ml affinity gel column (0.7 X 2.5 cm) possessing a covalently linked acarbose ligand (1 mg aca...

journal_title：Biotechnology and applied biochemistry

authors： Ono K,Smith EE

更新日期：1986-04-01 00:00:00

abstract：:The antifungal activity of iturin A and its interaction with erythrocyte membranes were enhanced in the presence of surfactin. The modification of the properties of iturin A was explained by the formation of mixed iturin A-surfactin micelles. Such mixed micelles were easily generated when both lipopeptides were in aqu...

journal_title：Biotechnology and applied biochemistry

authors： Thimon L,Peypoux F,Maget-Dana R,Roux B,Michel G

更新日期：1992-10-01 00:00:00

abstract：:Glutamate dehydrogenase (GDH) and alcohol dehydrogenase (ADH) have been encapsulated in sheep and human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At a fixed enzyme level in the dialysis bag (100 units/ml of RBCs), a significant encapsulation yield was observed for ADH, both in human ...

journal_title：Biotechnology and applied biochemistry

authors： Sanz S,Pinilla M,Garín M,Tipton KF,Luque J

更新日期：1995-10-01 00:00:00

abstract：:A sensitive sandwich enzyme immunoassay (e.i.a.) for serrapeptase (TSP), an orally available anti-inflammatory proteinase, was established using affinity-purified anti-TSP rabbit IgG and its Fab' fragment conjugated with horseradish peroxidase as the first and the second antibodies respectively. TSP in the plasma was ...

journal_title：Biotechnology and applied biochemistry

authors： Moriya N,Nakata M,Nakamura M,Takaoka M,Iwasa S,Kato K,Kakinuma A

更新日期：1994-08-01 00:00:00

abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...

journal_title：Biotechnology and applied biochemistry

authors： Ramström M,Zuberovic A,Grönwall C,Hanrieder J,Bergquist J,Hober S

更新日期：2009-02-01 00:00:00

abstract：:The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With ...

journal_title：Biotechnology and applied biochemistry

authors： Hou KC,Zaniewski R

更新日期：1990-06-01 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is a hematologic malignancy characterized by excessive growth of myeloid cells and their progenitors. The proportion of spectrin dimers compared to tetramers extracted from membranes at 4 degrees C, under low ionic strength conditions, increased in CML erythrocytes. These also displa...

journal_title：Biotechnology and applied biochemistry

authors： Basu J,Kundu M,Rakshit MM,Chakrabarti P

更新日期：1990-10-01 00:00:00

abstract：:A kinetic model was developed to describe recombinant human protein disulphide isomerase (rhPDI)-assisted folding of a substrate protein, C125A recombinant human interleukin-2 (C125A rhIL-2). A series of progress curves showing native C125A rhIL-2 formation under different reaction conditions were generated. Non-linea...

journal_title：Biotechnology and applied biochemistry

authors： Du C,Wolfe JL

更新日期：2003-04-01 00:00:00

abstract：:The protective antigen (PA) component of anthrax toxin mediates delivery of either lethal factor (LF) or oedema factor into the cytosol of mammalian cells. The N-terminal domain of LF(1-254) (amino acids 1-254 of LF) binds to PA and, when fused to heterologous proteins, delivers such proteins into the cytosol. In the ...

journal_title：Biotechnology and applied biochemistry

authors： Sharma M,Khanna H,Arora N,Singh Y

更新日期：2000-08-01 00:00:00

abstract：:The use of the recombinant BoNT/A-Hc (carboxylic domain of the Clostridium botulinum neurotoxin heavy chain) has been proposed as a vaccine candidate for botulism. This fragment contains the principle protective antigenic determinant. In the present study, in order to maximize recombinant protein expression, after ver...

journal_title：Biotechnology and applied biochemistry

authors： Yari K,Fatemi SS,Tavallaei M

更新日期：2010-05-24 00:00:00

abstract：:Arylacetonitrile-hydrolyzing nitrilase (E.C. 3.5.5.5) of Alcaligenes sp. MTCC 10675 has been purified by up to 46-fold to homogeneity and 32% yield using ammonium sulfate fractionation, Sephacryl S-300 gel permeation, and anion exchange chromatography. The molecular weight of the native enzyme was estimated to be 520 ...

journal_title：Biotechnology and applied biochemistry

authors： Bhatia SK,Mehta PK,Bhatia RK,Bhalla TC

更新日期：2014-07-01 00:00:00

abstract：:c-Jun is a highly oncogenic transcription factor involved in the development of different types of cancer. In the present study we have generated c-Jun-binding-affinity proteins from a phage-displayed library of so-called 'Affibody ligands', developed by combinatorial engineering of a non-immunoglobulin-based scaffold...

journal_title：Biotechnology and applied biochemistry

authors： Lundberg E,Brismar H,Gräslund T

更新日期：2009-01-01 00:00:00