Abstract:
:A three-layered simulation methodology is described that rapidly evaluates biomanufacturing process options. In each layer, inferior options are screened out, while more promising candidates are evaluated further in the subsequent, more refined layer, which uses more rigorous models that require more data from time-consuming experimentation. Screening ensures laboratory studies are focused only on options showing the greatest potential. To simplify the screening, outputs of production level, cost and time are combined into a single value using multi-attribute-decision-making techniques. The methodology was illustrated by evaluating alternatives to an FDA (U.S. Food and Drug Administration)-approved process manufacturing rattlesnake antivenom. Currently, antivenom antibodies are recovered from ovine serum by precipitation/centrifugation and proteolyzed before chromatographic purification. Alternatives included increasing the feed volume, replacing centrifugation with microfiltration and replacing precipitation/centrifugation with a Protein G column. The best alternative used a higher feed volume and a Protein G step. By rapidly evaluating the attractiveness of options, the methodology facilitates efficient and cost-effective process development.
journal_name
Biotechnol Appl Biochemjournal_title
Biotechnology and applied biochemistryauthors
Chhatre S,Francis R,O'Donovan K,Titchener-Hooker NJ,Newcombe AR,Keshavarz-Moore Edoi
10.1042/ba20070018subject
Has Abstractpub_date
2007-10-01 00:00:00pages
65-78issue
Pt 2eissn
0885-4513issn
1470-8744journal_volume
48pub_type
杂志文章abstract::The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin inj...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1495
更新日期:2017-05-01 00:00:00
abstract::Green fluorescent protein (GFP) is a globular protein used as biosensor and biomarker in medical and industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-d-1-thiogalactopyranoside (IPTG), the number of GFP applications are still scarce. This...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1749
更新日期:2019-07-01 00:00:00
abstract::A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN-DI) that happened during the long-term storage process. It was found that FMN-DI did not simpl...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1607
更新日期:2018-05-01 00:00:00
abstract::Diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase has been isolated previously using classical protein isolation techniques [A. Guranowski and S. Blanquet (1985) J. Biol. Chem. 260, 3542-3547]. A protein A-Sepharose immunoaffinity column was prepared to simplify the purification procedure. The immunoaffini...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::Melamine adulteration of food is a public health concern. It has been seen that melamine causes disease in many organs. Melamine-induced kidney disease is a well-recognized clinicopathological entity. Inflammation is thought to be important in melamine-induced pathology. Melamine is expected to bind with albumin becau...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1512
更新日期:2017-07-01 00:00:00
abstract::Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an au...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1876
更新日期:2020-05-01 00:00:00
abstract::Mustard tuber wastewater is characterized by high salinity and high organic content that is potentially detrimental to the biological treatment system and affects the treatment efficiency accordingly. The experiment used the integrated bioreactor to reduce much of the organics in mustard tuber wastewater, and found th...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1396
更新日期:2016-07-01 00:00:00
abstract::The interpretation of the circular dichroism (c.d.) spectra of proteins to date requires additional secondary structural information of the proteins to be analysed (e.g., X-ray or n.m.r. data). Therefore these methods are inappropriate for a c.d. database whose secondary structures are unknown, as in the case of the m...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:
更新日期:1993-10-01 00:00:00
abstract::This study was designed to investigate the protective effects of Acanthus ilicifolius alkaloid A [4-hydroxy-2-benzoxazolone (HBOA)] and its acetylated derivatives including 4-acetoxy-2-benzoxazolone (TC-2) and 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride (CCl4 )-induced liver fibrosis in rats. Spr...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1292
更新日期:2015-07-01 00:00:00
abstract::Restoration of insulin secretion by transplantation of isolated islets is a treatment for type Ι diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate e...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1295
更新日期:2015-07-01 00:00:00
abstract::In this study, the whole proteins from a Chinese three-striped box turtle (Cuora trifasciata) were extracted and hydrolyzed using three proteases (alcalase, papain, and protamex). By orthogonal experiments, the optimal hydrolysis conditions for producing peptides with the highest cancer cells growth inhibition activit...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1548
更新日期:2017-11-01 00:00:00
abstract::Lipases are surface-active enzymes, acting on their substrates at the polar/nonpolar interface in emulsions. This study was aimed to test whether their activity, specificity, and the rates of formation/degradation of the various hydrolysis intermediates (i.e., mono- and diglycerides of interest as surface-active agent...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1761
更新日期:2019-07-01 00:00:00
abstract::Gene doping can be easily concealed since its product is similar to endogenous protein, making its effective detection very challenging. In this study, we selected insulin-like growth factor I (IGF-I) exogenous gene for gene doping detection. First, the synthetic IGF-I gene was subcloned to recombinant adeno-associate...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1518
更新日期:2017-07-01 00:00:00
abstract::A simplified and low-cost method, boiling lysis, was developed and used to purify the truncated DNA polymerase Stoffel fragment after overproduction of the enzyme in Escherichia coli. The method is based on the thermostable properties of the Stoffel fragment. Conditions such as the boiling time and number of cycles em...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20070114
更新日期:2008-06-01 00:00:00
abstract::Cinnamyl acetate was synthesized using immobilized lipase through transesterification between ethyl acetate and cinnamyl alcohol. In the solvent-free system, ethyl acetate acted as not only the acyl donor but also as the solvent of cinnamyl alcohol. Conversion (90.06%) was achieved after 3 H when transesterification w...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1023
更新日期:2012-07-01 00:00:00
abstract::Rapid progress has been made in the design and synthesis of oligomers and polymers that emulate the properties of natural proteins. Molecular bioengineering offers the chance to design and produce artificial polymeric proteins with tailored polymeric properties. The elastin-like polypeptides are a well-defined family ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20050114
更新日期:2005-12-01 00:00:00
abstract::The purpose of the present study was to investigate the effects of garlic (Allium sativum L.) on the diabetic nephropathy and oxidative stress induced by STZ (streptozotocin) in rats. Diabetes was induced in Male Sprague-Dawley rats by administering a single intraperitoneal injection of STZ (60 mg/kg of body weight). ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20080086
更新日期:2009-03-01 00:00:00
abstract::Ethylene glycol bis (beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA)-calcium buffer is widely used in various calcium-dependent reactions where free calcium concentrations of 1 microM or less are desirable. The free calcium concentration is calculated from the association constant of EGTA . Ca2- and serves as the ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::Glutamate dehydrogenase (GDH) and alcohol dehydrogenase (ADH) have been encapsulated in sheep and human red blood cells (RBCs) by a hypotonic dialysis/isotonic resealing procedure. At a fixed enzyme level in the dialysis bag (100 units/ml of RBCs), a significant encapsulation yield was observed for ADH, both in human ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::Recently, hPNAS-4 [human PNAS-4; also known as C1orf121 (Genbank accession number NP-057160)] was reported to be a novel pro-apoptotic protein in mammalian cells. However, at present there is little information about hPNAS-4 and its molecular mechanisms. In our present studies, the hPNAS-4 gene was first cloned into t...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20090111
更新日期:2010-02-11 00:00:00
abstract::A truncated precursor form of prostate-specific antigen (PSA), [-2]proPSA, is a well-known biomarker for prostate cancer. To develop a biomarker assay, highly purified [-2]proPSA is required as a standard reference and for generation of a specific antibody. In this study, we generated an efficient mammalian expression...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1481
更新日期:2017-05-01 00:00:00
abstract::Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new ti...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章,评审
doi:
更新日期:1990-10-01 00:00:00
abstract::Mechano-growth factor (MGF), an alternative splicing variant of insulin-like growth factor-1 (IGF-1) gene, promotes cell proliferation and inhibits cell differentiation. It also plays an important role in tumor development. It is important to optimize the production process and achieve MGF protein because there is no ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1312
更新日期:2015-09-01 00:00:00
abstract::Biological mineralization is a highly programmed process in which inorganic minerals reassociate under the strict control of the extracellular matrix to form minerals with special functions and patterns. Shells are biominerals, and their synthesis is finely regulated by organic matrix including matrix proteins, polysa...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1002/bab.1752
更新日期:2019-07-01 00:00:00
abstract::In order to impart an ability for receptor-independent transmembrane transfer to water-soluble proteins, it has been suggested that they be hydrophobized by lipid groups (fatty acids, etc.). To this end, systems of reversed micelles of surfactants in organic solvents were used as reaction media for protein modificatio...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::As a model of cell-cell recognition processes, the association processes of a polysaccharide (mannan)-carrying liposome with a lectin (Concanavalin A, Con A)-carrying polymerized liposome were followed by turbidimetry. The association process was strongly inhibited by the addition of a low molecular weight sugar, meth...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1111/j.1470-8744.1992.tb00195.x
更新日期:1992-02-01 00:00:00
abstract::An extremely thermostable UDP-GlcNAc pyrophosphorylase has been purified from Thermus caldophilus GK24 by chromatographic methods including ion-exchange, hydrophobic interaction, and affinity chromatographies. The specific activity of the enzyme was enriched 41.8-fold, with a recovery of 2%. The molecular mass of the ...
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::A novel, improved dual bacterial-expression system, designed for large-scale generation of high-quality polyclonal antibody preparations intended for proteomics research, is presented. The concept involves parallel expression of cDNA-encoded proteins, as a fusion with two different tags in two separate vector systems....
journal_title:Biotechnology and applied biochemistry
pub_type: 杂志文章
doi:10.1042/BA20030091
更新日期:2003-12-01 00:00:00