Effect of silica nanoparticles on microbial biomass and silica availability in maize rhizosphere.

abstract：:Pancreatic duodenum homeobox protein-1 (PDX1) is a master regulatory gene in pancreatic development. Reprogramming of mesenchymal stem cells (MSCs) is a promising tool for producing insulin-producing cells. In this study, lentivirus harboring PDX1 (LV-PDX1) has been used for persistence gene expression in MSCs. The ob...

journal_title：Biotechnology and applied biochemistry

authors： Talebi S,Aleyasin A,Soleimani M,Massumi M

更新日期：2012-05-01 00:00:00

abstract：:A simple, specific, sensitive and reproducible ELISA has been developed to quantify the level of CPS (capsular polysaccharide) production in supernatants of Streptococcus pneumoniae cell cultures. CPSs from Strep. pneumoniae have been widely used as vaccine antigens. The quantification method is based on two type-23F ...

journal_title：Biotechnology and applied biochemistry

authors： Cruz-Leal Y,Menéndez T,Coizeau E,Espinosa RR,Canaan L,Blanco F,Carmenate T,Chang J,Quiñones D,Tamargo I,Cremata J,Verez-Bencomo V,Guillén G

更新日期：2006-05-01 00:00:00

abstract：:Green fluorescent protein (GFP) is a globular protein used as biosensor and biomarker in medical and industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-d-1-thiogalactopyranoside (IPTG), the number of GFP applications are still scarce. This...

journal_title：Biotechnology and applied biochemistry

authors： Lopes C,Dos Santos NV,Dupont J,Pedrolli DB,Valentini SR,de Carvalho Santos-Ebinuma V,Pereira JFB

更新日期：2019-07-01 00:00:00

abstract：:In the present study, recombinant HCV (hepatitis C virus) core proteins enhanced the immune response elicited by a co-delivered DNA vaccine encoding HCV core and envelope proteins. A mixture of the plasmid pIDKE2 and Co.173, a protein comprising the first 173 amino acids of HCV core, in particular induces a strong hum...

journal_title：Biotechnology and applied biochemistry

authors： Alvarez-Lajonchere L,González M,Alvarez-Obregón JC,Guerra I,Viña A,Acosta-Rivero N,Musacchio A,Morales J,Dueñas-Carrera S

更新日期：2006-04-01 00:00:00

abstract：:Lipases are surface-active enzymes, acting on their substrates at the polar/nonpolar interface in emulsions. This study was aimed to test whether their activity, specificity, and the rates of formation/degradation of the various hydrolysis intermediates (i.e., mono- and diglycerides of interest as surface-active agent...

journal_title：Biotechnology and applied biochemistry

authors： Carpen A,Bonomi F,Iametti S,Marengo M

更新日期：2019-07-01 00:00:00

abstract：:hES (human embryonic stem) cells hold tremendous potential in the newly emerging field of regenerative medicine, in addition to being a useful tool in basic scientific research and for pharmacological and cytotoxicity screening. However, an essential prerequisite for the future widespread application of hES cells are ...

journal_title：Biotechnology and applied biochemistry

authors： Heng BC,Kuleshova LL,Bested SM,Liu H,Cao T

更新日期：2005-04-01 00:00:00

abstract：:In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carra...

journal_title：Biotechnology and applied biochemistry

authors： Koklukaya SZ,Sezer S,Aksoy S,Hasirci N

更新日期：2016-09-01 00:00:00

abstract：:Diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) phosphorylase has been isolated previously using classical protein isolation techniques [A. Guranowski and S. Blanquet (1985) J. Biol. Chem. 260, 3542-3547]. A protein A-Sepharose immunoaffinity column was prepared to simplify the purification procedure. The immunoaffini...

journal_title：Biotechnology and applied biochemistry

authors： Avila DM,Kaushal V,Barnes LD

更新日期：1990-06-01 00:00:00

abstract：:The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a plant species known for its production of TIAs (terpenoid indole alkaloids), many of which are pharmaceutically important. Ajmalicine and serpentine are prescribed for the treatment of hypertension, whereas the bisindoles vinblastine, vincristine and 3',...

journal_title：Biotechnology and applied biochemistry

authors： Zhou ML,Shao JR,Tang YX

更新日期：2009-04-01 00:00:00

abstract：:The immobilization of F(0)F(1)-ATPase in uniform orientation is reported. The biotinylated and histidine-tagged subunits of the bacterial F(0)F(1)-ATPase complex were used for immobilization of the complex on artificial semi-permeable membranes resulting in 88+/-7.8 and 72+/-5.2% coupling of the enzymes. The immobiliz...

journal_title：Biotechnology and applied biochemistry

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2004-06-01 00:00:00

abstract：:c-Jun is a highly oncogenic transcription factor involved in the development of different types of cancer. In the present study we have generated c-Jun-binding-affinity proteins from a phage-displayed library of so-called 'Affibody ligands', developed by combinatorial engineering of a non-immunoglobulin-based scaffold...

journal_title：Biotechnology and applied biochemistry

authors： Lundberg E,Brismar H,Gräslund T

更新日期：2009-01-01 00:00:00

abstract：:Mouse and human erythrocytes are inherently different with respect to slow dialysis encapsulation used in preparing carrier erythrocytes. Fluorescein isothiocyanate (FITC)-dextran was added to five different stages of the encapsulation process to discern when endocytic vesiculation occurred. Mouse erythrocytes were mu...

journal_title：Biotechnology and applied biochemistry

authors： DeLoach JR,Droleskey RE

更新日期：1993-08-01 00:00:00

abstract：:Lys and Leu were generally considered as the key amino acids for brewer's yeast during beer brewing. In the present study, peptide Lys-Leu and a free amino acid (FAA) mixture of Lys and Leu (Lys + Leu) were supplemented in 24 °P wort to examine their effects on physiological activity and fermentation performance of br...

journal_title：Biotechnology and applied biochemistry

authors： Yang H,Zong X,Cui C,Mu L,Zhao H

更新日期：2018-07-01 00:00:00

abstract：:Although most PCRs would produce proper PCR products when first tried, a general optimization is required to yield the best results. This optimization is often achieved by changing one factor at a time. However, this may lead to suboptimal results, since interactions between conditions are difficult to detect with thi...

journal_title：Biotechnology and applied biochemistry

authors： Niens M,Spijker GT,Diepstra A,te Meerman GJ

更新日期：2005-10-01 00:00:00

abstract：:The use of glutaraldehyde (GTA) for cross-linking biological tissue implants has a number of undesirable side effects, including cytotoxicity and induction of calcification. In an attempt to find an improved cross-linking agent for tissue implants, we have evaluated a number of cross-linking procedures shown previousl...

journal_title：Biotechnology and applied biochemistry

authors： Simmons DM,Kearney JN

更新日期：1993-02-01 00:00:00

abstract：:Flat panels are the most spread type of photobioreactors for studying light effects on a microalgae culture. Their low thickness, usually between 1 and 3 cm, aims at ensuring light homogeneity across the culture. Yet because optical density has to remain very low, studies are still limited to low cell density cultures...

journal_title：Biotechnology and applied biochemistry

authors： Jiang W,Levasseur W,Casalinho J,Martin T,Puel F,Perré P,Pozzobon V

更新日期：2020-02-03 00:00:00

abstract：:Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immedia...

journal_title：Biotechnology and applied biochemistry

authors： Qiu C,Wang X,Zhang X,Li Z,Zhou Y,Kang J

更新日期：2020-08-15 00:00:00

abstract：:Tuberculosis has long been the scourge of humanity, claiming millions of lives. The family of PE_PGRS gene has been attributed to the Mycobacterium tuberculosis pathogenesis over the past few decades. The gene of PE_PGRS family proteins are most often clustered in a region of the genome often as overlapping genes and ...

journal_title：Biotechnology and applied biochemistry

authors： Meena LS

更新日期：2015-03-01 00:00:00

abstract：:Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an au...

journal_title：Biotechnology and applied biochemistry

authors： Jantra J,Zór K,Sanders M,De Saeger S,Hedström M,Mattiasson B

更新日期：2020-05-01 00:00:00

abstract：:We evaluated a new protocol for measurement of cyclosporine A (CsA) 2 H after dose (C2) on the V-Twin® analyzer. Imprecision, recovery, and linearity were determined using CsA-spiked blood pools. Accuracy was evaluated using specimens from renal, cardiac, and liver transplant patients, and results were compared with t...

journal_title：Biotechnology and applied biochemistry

authors： Morjana N,Yau H,Rea D,Ruttle D,Jones H,Siefring G Jr,Christenson R

更新日期：2011-11-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) still represents a major problem to the swine industry worldwide, causing high mortality rates in infected animals. Virus-like particles (VLPs) have gained attention for vaccine development, serving both as scaffolds for epitope expression and immune response enhancers. The commercial ...

journal_title：Biotechnology and applied biochemistry

authors： Aguilera BE,Chávez-Calvillo G,Elizondo-Quiroga D,Jimenez-García MN,Carrillo-Tripp M,Silva-Rosales L,Hernández-Gutiérrez R,Gutiérrez-Ortega A

更新日期：2017-05-01 00:00:00

abstract：:[Glu34]-thymopoietin II fragment 32-45 and its three analogs were prepared by substitution of the amino acid residue at position 37. These peptides were synthesized by a conventional solution method, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid in the presence of ...

journal_title：Biotechnology and applied biochemistry

authors： Abiko T,Shishido H,Sekino H

更新日期：1986-04-01 00:00:00

abstract：:In the present study, the growth and key metabolic features of a gene-transfected Drosophila melanogaster (fruitfly) S2 (Schneider 2) cell population (S2AcRVGP cells), cultured in Sf900-II medium, have been evaluated to provide substantial support for the development of a bioprocess to produce RVGP (rabies-virus glyco...

journal_title：Biotechnology and applied biochemistry

authors： Swiech K,da Silva CS,Arantes MK,dos Santos AS,Astray RM,Pereira CA,Suazo CA

更新日期：2008-01-01 00:00:00

abstract：:Future developments in gene therapy and DNA vaccination depend on cost-effective large-scale production of pharmaceutical-grade pDNA (plasmid DNA). Given the large amount of impurities present in the feedstock, purification processes that have high specificity and capacity at a moderate cost are required. In the prese...

journal_title：Biotechnology and applied biochemistry

authors： Frerix A,Müller M,Kula MR,Hubbuch J

更新日期：2005-08-01 00:00:00

abstract：:Human thymosin alpha1 is an effective immune system enhancer for the treatment of cancer and viral diseases. Therefore the development of new methods for its synthesis is an urgent problem. In the present work, we propose an efficient scalable scheme for the production of recombinant thymosin alpha1. We used an expres...

journal_title：Biotechnology and applied biochemistry

authors： Esipov RS,Stepanenko VN,Beyrakhova KA,Muravjeva TI,Miroshnikov AI

更新日期：2010-05-19 00:00:00

abstract：:The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25-30 degrees C with a maximum at 30 degrees C. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved m...

journal_title：Biotechnology and applied biochemistry

authors： Son HJ,Heo MS,Kim YG,Lee SJ

更新日期：2001-02-01 00:00:00

abstract：:Microparticles have been used for delivery of chemo- and radio-therapeutic agents to tumours in patients for more than a decade, whereas cationic liposomes have been used for introducing nucleic acids into mammalian cells for the same period of time. This minireview discusses the potential of delivering therapeutic ON...

journal_title：Biotechnology and applied biochemistry

authors： Dass CR

更新日期：2004-10-01 00:00:00

abstract：:A rapid, simple and sensitive fluorometric assay method for the determination of nitrilase activity is described. 3-Cyanopyridine was hydrolysed to nicotinic acid by Rhodococcus rhodochrous and the liberated NH(3) was allowed to react with buffered o -phthaldialdehyde-2-mercaptoethanol solution (pH 7.4) to form a fluo...

journal_title：Biotechnology and applied biochemistry

authors： Banerjee A,Sharma R,Banerjee UC

更新日期：2003-06-01 00:00:00

abstract：:Human erythrocytes from ten patients with chronic obstructive pulmonary disease (COPD) were loaded with increasing amounts of dexamethasone 21-phosphate and were re-infused into the original donors. Drug-loaded erythrocytes acted as circulating bioreactors, converting the non-diffusible dexamethasone 21-phosphate into...

journal_title：Biotechnology and applied biochemistry

authors： Rossi L,Serafini S,Cenerini L,Picardi F,Bigi L,Panzani I,Magnani M

更新日期：2001-04-01 00:00:00

abstract：:The survival of inoculated microbes is critical for successful bioaugmentation in wastewater treatment. The influence of readily available nutrients (RANs) on the colonization of two functional bacteria, Pseudomonas putida M9, a strong biofilm-forming strain, and Comamonas testosteroni A3, a 3,5-dinitrobenzoic acid (3...

journal_title：Biotechnology and applied biochemistry

authors： Cheng Z,Meng X,Xie L,Xu H,Li M

更新日期：2015-09-01 00:00:00