当前位置： SCI文献检索 > Biomed Research International期刊下所有文献 > LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity.

LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity.

Abstract：

:Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage. The gene encoding LraI endonuclease was cloned and overexpressed in E. coli. Purified enzyme showed the highest specific activity at lower temperatures (between 13°C and 37°C) and was stable after storage at -20°C in 50% glycerol. The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher. The recognition and cleavage sequence for LraI restriction enzyme was determined as 5'-G/AATTC-3', indicating that LraI restriction enzyme is an isoschizomer of EcoRI. In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5'-A/AATTC-3', leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector. Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.

journal_name

Biomed Res Int

journal_title

BioMed research international

authors

Miljkovic M,Malesevic M,Filipic B,Vukotic G,Kojic M

doi

10.1155/2018/5657085

subject

Has Abstract

pub_date

2018-01-29 00:00:00

pages

5657085

eissn

2314-6133

issn

2314-6141

journal_volume

2018

pub_type

杂志文章

相关文献

Biomed Research International文献大全
  • Age-Related Changes of Elastic Fibers in Shoulder Capsule of Patients with Glenohumeral Instability: A Pilot Study.

    abstract：Background:Recurrent shoulder dislocations occur much more frequently in adolescents than in the older population but a clear explanation of this incidence does not exist. The aim of the present study was to define the age-related distribution of the elastic fibers (EFs) in the shoulder capsule's extracellular matrix a...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2018/8961805

    authors： Castagna A,Cesari E,Gigante A,Di Matteo B,Garofalo R,Porcellini G

    更新日期：2018-07-18 00:00:00

  • A Fatty Acids Mixture Reduces Anxiety-Like Behaviors in Infant Rats Mediated by GABAA Receptors.

    abstract：:Fatty acids (C6-C18) found in human amniotic fluid, colostrum, and maternal milk reduce behavioral indicators of experimental anxiety in adult Wistar rats. Unknown, however, is whether the anxiolytic-like effects of fatty acids provide a natural mechanism against anxiety in young offspring. The present study assessed ...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2017/8798546

    authors： Bernal-Morales B,Cueto-Escobedo J,Guillén-Ruiz G,Rodríguez-Landa JF,Contreras CM

    更新日期：2017-01-01 00:00:00

  • The Effect of Immigration on Adverse Perinatal Outcomes: Analysis of Experiences at a Turkish Tertiary Hospital.

    abstract：Introduction:In literature, it is well documented that migration is associated with adverse perinatal outcomes in many countries over the world. But in Turkey, health care providers and obstetricians had to face the effects of migration for the first time after civil war in Syria. Hence, this situation motivated us to ...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2019/2326797

    authors： Col Madendag I,Eraslan Sahin M,Madendag Y,Sahin E,Demir MB,Ozdemir F,Acmaz G,Muderris II

    更新日期：2019-11-05 00:00:00

  • LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p.

    abstract：:Cervical cancer is one of the malignant tumors that seriously threaten women's health. The mechanism of development needs to be deeply studied. In recent years, lncRNA has been identified as one of the important factors affecting the malignant progression of tumors. In this study, we illustrated the important mechanis...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/4351671

    authors： Hu T,Zhang Q,Gao L

    更新日期：2020-01-03 00:00:00

  • Effects of ACEIs and ARBs on the Residual Renal Function in Peritoneal Dialysis Patients: A Meta-Analysis of Randomized Controlled Trials.

    abstract：Background:In peritoneal dialysis (PD) patients, whether angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin receptor blockers (ARBs) could protect residual renal function is still controversial. To assess the effects of ACEIs and ARBs on the residual renal function and cardiovascular (CV) events in perito...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2020/6762029

    authors： Ding L,Yang J,Li L,Yang Y

    更新日期：2020-09-23 00:00:00

  • Vitamin D signaling in myogenesis: potential for treatment of sarcopenia.

    abstract：:Muscle mass and strength progressively decrease with age, which results in a condition known as sarcopenia. Sarcopenia would lead to physical disability, poor quality of life, and death. Therefore, much is expected of an effective intervention for sarcopenia. Epidemiologic, clinical, and laboratory evidence suggest an...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2014/121254

    authors： Wagatsuma A,Sakuma K

    更新日期：2014-01-01 00:00:00

  • Identification of Key Genes and Long Noncoding RNA-Associated Competing Endogenous RNA (ceRNA) Networks in Early-Onset Preeclampsia.

    abstract：Background:Preeclampsia (PE) is a pregnancy-specific hypertension syndrome and is one of the leading causes of maternal and perinatal morbidity and mortality. Long noncoding RNAs (lncRNAs) have been reported to be abnormally expressed in many diseases, including preeclampsia. The present study is aimed at identifying t...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/1673486

    authors： Zhang Z,Wang P,Zhang L,Huang C,Gao J,Li Y,Yang B

    更新日期：2020-06-05 00:00:00

  • Lactulose Hydrogen Breath Test Result Is Associated with Age and Gender.

    abstract：:Small intestinal bacterial overgrowth (SIBO) is associated with chronic gastrointestinal diseases and structural/functional abnormalities of the gastrointestinal tract. SIBO's association with clinical characteristics is unclear. This study investigates the association between clinical factors and SIBO according to la...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2016/1064029

    authors： Newberry C,Tierney A,Pickett-Blakely O

    更新日期：2016-01-01 00:00:00

  • A Prospective Randomized Study of Brain Tissue Oxygen Pressure-Guided Management in Moderate and Severe Traumatic Brain Injury Patients.

    abstract：:The purpose of this study was to compare the effect of PbtO2-guided therapy with traditional intracranial pressure- (ICP-) guided treatment on the management of cerebral variables, therapeutic interventions, survival rates, and neurological outcomes of moderate and severe traumatic brain injury (TBI) patients. From 20...

    journal_title：BioMed research international

    pub_type： 杂志文章,随机对照试验

    doi：10.1155/2015/529580

    authors： Lin CM,Lin MC,Huang SJ,Chang CK,Chao DP,Lui TN,Ma HI,Liu MY,Chung WY,Shih YH,Tsai SH,Chiou HY,Lin MR,Jen SL,Wei L,Wu CC,Lin EY,Liao KH,Chiang YH,Chiu WT,Lin JW

    更新日期：2015-01-01 00:00:00

  • Does loop electrosurgical excision procedure of the uterine cervix affect anti-Müllerian hormone levels?

    abstract：BACKGROUND:A delayed time to pregnancy was recently reported for women who had a loop electrosurgical excision procedure (LEEP) to remove cervical intraepithelial neoplasia (CIN) grade 2 or 3. The objective of the current study was to determine if treatment of CIN with LEEP is associated with decreased levels of anti-M...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2014/875438

    authors： Sklavos MM,Spracklen CN,Saftlas AF,Pinto LA

    更新日期：2014-01-01 00:00:00

  • Effects of Physical Activity Programs on the Improvement of Dementia Symptom: A Meta-Analysis.

    abstract：:Objective. To confirm that physical activity program improves the symptoms of dementia and the most effective physical activity was selected to help establish exercise programs. Methods. Three databases, PubMed, Science Direct, and Willey online, were used to collect articles. The databases were published between Janu...

    journal_title：BioMed research international

    pub_type： 杂志文章,meta分析

    doi：10.1155/2016/2920146

    authors： Lee HS,Park SW,Park YJ

    更新日期：2016-01-01 00:00:00

  • MiR-451 Promotes Cell Proliferation and Metastasis in Pancreatic Cancer through Targeting CAB39.

    abstract：:Emerging evidence shows that microRNAs (miRNAs) play important roles in the regulation of various biological and pathologic processes in human cancers and the aberrant expression of miRNAs contributes to the tumor development. In this study, our findings indicate that miR-451 is significantly overexpressed in pancreat...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2017/2381482

    authors： Guo R,Gu J,Zhang Z,Wang Y,Gu C

    更新日期：2017-01-01 00:00:00

  • A Computer-Aided Analysis Method of SPECT Brain Images for Quantitative Treatment Monitoring: Performance Evaluations and Clinical Applications.

    abstract：:The objective and quantitative analysis of longitudinal single photon emission computed tomography (SPECT) images are significant for the treatment monitoring of brain disorders. Therefore, a computer aided analysis (CAA) method is introduced to extract a change-rate map (CRM) as a parametric image for quantifying the...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2017/1962181

    authors： Zheng X,Wei W,Huang Q,Song S,Wan J,Huang G

    更新日期：2017-01-01 00:00:00

  • In Vivo Release of Vancomycin from Calcium Phosphate Cement.

    abstract：:Calcium phosphate cement (CPC) has good release efficiency and has therefore been used as a drug delivery system for postoperative infection. The release profile of CPC has mainly been evaluated by in vitro studies, which are carried out by immersing test specimens in a relatively large amount of solvent. However, it ...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2018/4560647

    authors： Uchida K,Sugo K,Nakajima T,Nakawaki M,Takano S,Nagura N,Takaso M,Urabe K

    更新日期：2018-05-15 00:00:00

  • Extremely low frequency magnetic fields induce spermatogenic germ cell apoptosis: possible mechanism.

    abstract：:The energy generated by an extremely low frequency electromagnetic field (ELF-EMF) is too weak to directly induce genotoxicity. However, it is reported that an extremely low frequency magnetic field (ELF-MF) is related to DNA strand breakage and apoptosis. The testes that conduct spermatogenesis through a dynamic cell...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2014/567183

    authors： Lee SK,Park S,Gimm YM,Kim YW

    更新日期：2014-01-01 00:00:00

  • Influence of Polishing Methods on the Bonding Effectiveness and Durability of Different Resin Cements to Dentin.

    abstract：:The purpose of this study was to investigate the impact of polishing methods on the bonding effectiveness and durability of different resin cements to dentin. The dentin surfaces were either treated with a fine-grit diamond bur (polishing A) or further polished by polishing disks (polishing B), and then they were bond...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2018/9189354

    authors： Ren L,Li M,Pan Y,Meng X

    更新日期：2018-02-28 00:00:00

  • Effectiveness of Foot Biomechanical Orthoses to Relieve Patients Suffering from Plantar Fasciitis: Is the Reduction of Pain Related to Change in Neural Strategy?

    abstract：:Plantar fasciitis is a cause of chronic pain under the heel and bottom of the foot. One of the treatments to reduce pain consists of using plantar orthoses to address specific imbalances during foot placement or gait. The aim of the present study was to determine if reduction of pain with a treatment based on plantar ...

    journal_title：BioMed research international

    pub_type： 临床试验,杂志文章

    doi：10.1155/2018/3594150

    authors： Moyne-Bressand S,Dhieux C,Dousset E,Decherchi P

    更新日期：2018-12-12 00:00:00

  • Stereotactic radiosurgery with neoadjuvant embolization of larger arteriovenous malformations: an institutional experience.

    abstract：OBJECTIVE:This study investigates the safety and efficacy of a multimodality approach combining staged endovascular embolizations with subsequent SRS for the management of larger AVMs. METHODS:Ninety-five patients with larger AVMs were treated with staged endovascular embolization followed by SRS between 1996 and 2011...

    journal_title：BioMed research international

    pub_type： 临床试验,杂志文章

    doi：10.1155/2014/306518

    authors： Dalyai R,Theofanis T,Starke RM,Chalouhi N,Ghobrial G,Jabbour P,Dumont AS,Fernando Gonzalez L,Gordon DS,Rosenwasser RH,Tjoumakaris SI

    更新日期：2014-01-01 00:00:00

  • Netrins and their roles in placental angiogenesis.

    abstract：:Netrins, a family of laminin-related proteins, were originally identified as axonal guidance molecules. Subsequently, netrins were found to modulate various biological processes including morphogenesis, tumorogenesis, adhesion, and, recently, angiogenesis. In human placenta, the most vascularized organ, the presence o...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2014/901941

    authors： Dakouane-Giudicelli M,Alfaidy N,de Mazancourt P

    更新日期：2014-01-01 00:00:00

  • Danggui Buxue Tang Attenuates Tubulointerstitial Fibrosis via Suppressing NLRP3 Inflammasome in a Rat Model of Unilateral Ureteral Obstruction.

    abstract：:Inflammation significantly contributes to the progression of chronic kidney disease (CKD). This study aimed to characterize Danggui Buxue Tang (DBT) renoprotection and relationship with NOD-like receptors family pyrin domain-containing 3 (NLRP3) inflammasome expression in rats with unilateral ureteral obstruction (UUO...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2016/9368483

    authors： Wang L,Ma J,Guo C,Chen C,Yin Z,Zhang X,Chen X

    更新日期：2016-01-01 00:00:00

  • Quercetin Inhibits Inflammatory Response Induced by LPS from Porphyromonas gingivalis in Human Gingival Fibroblasts via Suppressing NF-κB Signaling Pathway.

    abstract：:Quercetin, a natural flavonol existing in many food resources, has been reported to be an effective antimicrobial and anti-inflammatory agent for restricting the inflammation in periodontitis. In this study, we aimed to investigate the anti-inflammatory effects of quercetin on Porphyromonas gingivalis (P. gingivalis) ...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2019/6282635

    authors： Xiong G,Ji W,Wang F,Zhang F,Xue P,Cheng M,Sun Y,Wang X,Zhang T

    更新日期：2019-08-20 00:00:00

  • The Emerging Role of lncRNAs in Spinal Cord Injury.

    abstract：:Spinal cord injury (SCI) is a highly debilitating disease and is increasingly being recognized as an important global health priority. However, the mechanisms underlying SCI have not yet been fully elucidated, and effective therapies for SCI are lacking. Long noncoding RNAs (lncRNAs), which form a major class of nonco...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2019/3467121

    authors： Wang F,Liu J,Wang X,Chen J,Kong Q,Ye B,Li Z

    更新日期：2019-10-15 00:00:00

  • A Review of NEPA, a Novel Fixed Antiemetic Combination with the Potential for Enhancing Guideline Adherence and Improving Control of Chemotherapy-Induced Nausea and Vomiting.

    abstract：:Combination antiemetic regimens targeting multiple molecular pathways associated with emesis have become the standard of care for prevention of chemotherapy-induced nausea and vomiting (CINV) related to highly and moderately emetogenic chemotherapies. Antiemetic consensus guidelines from several professional societies...

    journal_title：BioMed research international

    pub_type： 杂志文章,评审

    doi：10.1155/2015/651879

    authors： Hesketh PJ,Aapro M,Jordan K,Schwartzberg L,Bosnjak S,Rugo H

    更新日期：2015-01-01 00:00:00

  • Intestinal parasites of owned dogs and cats from metropolitan and micropolitan areas: prevalence, zoonotic risks, and pet owner awareness in northern Italy.

    abstract：:Intestinal parasites of dogs and cats are cosmopolitan pathogens with zoonotic potential for humans. Our investigation considered their diffusion in dogs and cats from northern Italy areas, specifically the metropolitan area of Milan and two micropolitan areas of neighboring provinces. It included the study of the lev...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2014/696508

    authors： Zanzani SA,Gazzonis AL,Scarpa P,Berrilli F,Manfredi MT

    更新日期：2014-01-01 00:00:00

  • Rural residents in China are at increased risk of exposure to tick-borne pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis.

    abstract：:As emerging tick born rickettsial diseases caused by A. phagocytophilum and E. chaffeensis, anaplasmosis and ehrlichiosis have become a serious threat to human and animal health throughout the world. In particular, in China, an unusual transmission of nosocomial cases of human granulocytic anaplasmosis occurred in Anh...

    journal_title：BioMed research international

    pub_type： 杂志文章,多中心研究

    doi：10.1155/2014/313867

    authors： Zhang L,Liu H,Xu B,Zhang Z,Jin Y,Li W,Lu Q,Li L,Chang L,Zhang X,Fan D,Cao M,Bao M,Zhang Y,Guan Z,Cheng X,Tian L,Wang S,Yu H,Yu Q,Wang Y,Zhang Y,Tang X,Yin J,Lao S,Wu B,Li J,Li W,Xu Q,Shi Y,Huan

    更新日期：2014-01-01 00:00:00

  • Coronal Imbalance after Selective Posterior Thoracic Fusion in Patients with Lenke 1 and 2 Adolescent Idiopathic Scoliosis.

    abstract：:Coronal decompensation is a common complication in Lenke 1 or 2 AIS patients after selective thoracic fusion (STF). However, the majority who developed immediately postoperative coronal decompensation experienced improvement and the related factors are not fully understood. The aim of this retrospective study was to i...

    journal_title：BioMed research international

    pub_type： 临床试验,杂志文章

    doi：10.1155/2018/3476425

    authors： Jiang H,Shao W,Xu E,Ji Z,Lin T,Meng Y,Ma J,Wang C,Gao R,Zhou X

    更新日期：2018-12-09 00:00:00

  • Nanoscale bonding between human bone and titanium surfaces: osseohybridization.

    abstract：:Until now, the chemical bonding between titanium and bone has been examined only through a few mechanical detachment tests. Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism ...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2015/960410

    authors： Kim JS,Kang SM,Seo KW,Nahm KY,Chung KR,Kim SH,Ahn JP

    更新日期：2015-01-01 00:00:00

  • Classification of Hallucal Sesamoid Bone Correlated with Hallux Valgus Severity.

    abstract：:The hallucal sesamoid bones (HSBs), having an important role in reducing load per unit area on the first metatarsal head, can be injured commonly which also affected the first metatarsophalangeal joint and the surrounding structure. Meanwhile, differences among each HSB type may be a major factor affecting the occurre...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/9658916

    authors： Zhang L,Wang J,Liu J,Luo J

    更新日期：2020-06-26 00:00:00

  • EGFR-AS1 Promotes Bladder Cancer Progression by Upregulating EGFR.

    abstract：:Long noncoding RNAs play an essential role in bladder cancer progression. The role of long noncoding RNA EGFR-AS1 in bladder cancer needs further study. We used clinical specimens to analyze the relationship between EGFR-AS1 and bladder cancer patients' characteristics. The functional experiments and mechanism studies...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2020/6665974

    authors： Wang A,Jiang A,Gan X,Wang Z,Huang J,Dong K,Liu B,Wang L,Chen M

    更新日期：2020-12-22 00:00:00

  • Organic anion transporter 5 renal expression and urinary excretion in rats with vascular calcification.

    abstract：:It has been described renal damage in rats with vascular calcification. The organic anion transporter 5 (Oat5) is only expressed in kidney, and its urinary excretion was proposed as potential early biomarker of renal injury. The aim of this study was to evaluate the Oat5 renal expression and its urinary excretion in a...

    journal_title：BioMed research international

    pub_type： 杂志文章

    doi：10.1155/2013/283429

    authors： Hazelhoff MH,Bulacio RP,Torres AM

    更新日期：2013-01-01 00:00:00