Abstract：
AIMS:Inducible nitric oxide synthase (iNOS) pathway has been in the limelight since its discovery as a key mediator in the process of liver fibrogenesis. Therefore, the objective of the current study was to elucidate the in vivo molecular mechanism underlying the hepatic preventive relevance of eugenol (EUG) and telmisartan (TEL) through iNOS pathway modulation against carbon tetrachloride (CCl4)-induced hepatic injury. METHODS:Sixty healthy male albino rats were used in this study. Serum aminotransferases activities and NO levels were assessed. Hepatic malondialdehyde (MDA), total nitrite/nitrate content and reduced glutathione (GSH) concentration were estimated. Liver NF-kB, TNF-α, IL-6 and iNOS proteins expressions were investigated by western blot assay. Histopathological examination was done. KEY FINDINGS:CCl4 resulted in damage to centrilobular regions of the liver, elevation of serum aminotransferases, rise in oxidative parameters level, and up-regulation of NF-kB, TNF-α, IL-6 as well as iNOS proteins expressions. Treatment of fibrotic rats with either EUG or TEL significantly alleviated CCl4-induced biochemical, inflammatory and histopathological changes. Moreover, the combined administration of EUG with TEL has an ameliorative effect which is greater than either of them alone. SIGNIFICANCE:In conclusion, the combination therapy between EUG and TEL is more effective than either drug alone which is attributed to suppression of NO production and iNOS protein expression. The results support that use of EUG and TEL exerts beneficial effects in the attenuation of CCl4-induced liver fibrosis in rats.
journal_name
Life Scijournal_title
Life sciencesauthors
Fathy M,Khalifa EMMA,Fawzy MAdoi
10.1016/j.lfs.2018.11.031subject
Has Abstractpub_date
2019-01-01 00:00:00pages
207-214eissn
0024-3205issn
1879-0631pii
S0024-3205(18)30744-6journal_volume
216pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Smokers have lower plasma concentrations of high-density lipoprotein (HDL) cholesterol and apolipoprotein A-I (apo A-I) compared with nonsmokers. To determine the molecular basis of this observation, the effect of activation of the aryl hydrocarbon receptor (AhR) on apo A-I gene expression was examined. MAIN METH...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.06.002
更新日期：2012-07-26 00:00:00
abstract：:The effects of cloricromene, a coumarine derivative, were studied in an anaesthetized rat model of coronary artery ligation (60 min) followed by reperfusion (60 min; MI/R). Sham operated rats were used as controls (Sham MI/R). Myocardial ischaemia-reperfusion injury produced a marked myocardial injury (necrotic area/a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90753-p
更新日期：1993-01-01 00:00:00
abstract：AIM:The purpose of this study was to investigate whether the flavonoid quercetin can prevent alterations in the behavioral tests and of cholinergic neurotransmission in rats submitted to the ethidium bromide (EB) experimental demyelination model during events of demyelination and remyelination. MAIN METHODS:Wistar rat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.03.033
更新日期：2014-05-17 00:00:00
abstract：:This study, conducted on isolated guinea pig ileum, was designed to establish the mechanism of presynaptic alpha 2-adrenoreceptor blockade by the tetramine disulfides, benextramine and pyrextramine. At 1 microM these drugs irreversibly blocked norepinephrine (NE)-induced inhibition of the twitch response to electrical...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90407-8
更新日期：1986-05-05 00:00:00
abstract：:Though chemokines of the CXC family are thought to play key roles in neoplastic transformation and tumor invasion, information about CXC chemokines in prostate cancer is sparse. To evaluate the involvement of CXC chemokines in prostate cancer, we analyzed the CXC coding mRNA of both chemokine ligands (CXCL) and chemok...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.08.019
更新日期：2006-03-13 00:00:00
abstract：:Voltage-dependent Ca2+ channels of fura-2-loaded ganglionic cells from the myenteric plexus of newborn rats were pharmacologically characterised. In contrast to completely dissociated myenteric cells, intact ganglia showed a stronger loading with the Ca2+-sensitive dye and a reproducible stimulation of the fura-2 sign...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.12.053
更新日期：2005-09-30 00:00:00
abstract：:Chronic administration of narcotic mu opioid agonists results in tolerance and dependence. We propose that agonist stimulation causes a gradual conversion of mu receptors to a constitutively active state (mu*) as a key step in tolerance and physical dependence. We provide evidence in support of the existence of mu* in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90022-1
更新日期：1994-01-01 00:00:00
abstract：:In the present study the possibility of provoking an irreversible blockade of P2-receptors in vivo by photolyzing ANAPP3 was investigated. ANAPP3 administered as an intraarterial infusion produced a short-lived blockade of P2-receptor mediated contractions of cat urinary bladders in situ. However, irradiation of the b...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90498-4
更新日期：1986-04-28 00:00:00
abstract：AIM:To investigate the cytotoxic effect of Peruvoside and mechanism of action in human cancers. MAIN METHODS:Cell viability was measured by MTT assay and the cell cycle arrest was identified by FACS. Real-time qPCR and western blotting studies were performed to identify important gene and protein expressions in the di...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117147
更新日期：2020-01-15 00:00:00
abstract：:Immunoreactive (ir) beta-endorphin (b-END) and dynorphin (DYN) in rat brain and pituitary were measured after food and water deprivation and from brains taken during either day or night. In other rats, eating and drinking were measured following lesions in the arcuate n. Ir-DYN levels are higher in hypothalamus and lo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90221-1
更新日期：1982-10-18 00:00:00
abstract：:A simple and sensitive method using high performance liquid chromatography with fluorometric detection has been developed for the identification and quantitation of the endogeneous tryptamine metabolites, indoleacetic acid (IAA) and tryptophol (TOL) in the normal mouse brain. The limits of sensitivity are 5pg for both...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90744-0
更新日期：1983-11-14 00:00:00
abstract：:Our previous research with long term cannabis users established an impaired ability to focus attention and filter out irrelevant information, which was progressive with the cumulative duration of exposure to cannabis. The current study examined these processes in a group of ex-cannabis users. The results suggested a p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00197-e
更新日期：1995-01-01 00:00:00
abstract：:An attenuation of adrenergic activity during the inhibition of endogenous angiotensin II formation was evaluated by determining plasma norepinephrine concentration after a single oral administration of captopril compared to that after nifedipine in essential hypertension. Captopril produced a fall in mean arterial pre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90415-8
更新日期：1991-01-01 00:00:00
abstract：AIM:Myocardial infarction (MI), an important cause of morbidity and mortality, can be followed by left ventricular dysfunction and cardiomyocyte loss. Cardiac repair mechanisms may subsequently improve left ventricular function. Exercise training has been suggested to have cardioprotective effects against MI damage, bu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.02.045
更新日期：2019-03-15 00:00:00
abstract：:Microvascular changes in the brain are significant causes of cerebral edema and ischemia injury. A number of studies suggest that angiotensin (Ang) II may be involved in the initiation and regulation of processes occurring in brain ischemia. We recently reported that Ang II injures brain microvascular endothelial cell...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.06.049
更新日期：2006-02-16 00:00:00
abstract：:Previous studies from this laboratory have shown that rats with experimental cirrhosis of the liver induced by the combined administration of oral phenobarbital and inhaled carbon tetrachloride show an hyperdynamic status with enhanced cardiac output (CO), and decreased mean arterial pressure (MAP) and peripheral vasc...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90531-x
更新日期：1986-07-21 00:00:00
abstract：:The antiarrhythmic and cardioprotective drug stobadine, possessing antioxidant and neuroprotective properties, was studied as to its in vitro effect on aggregation of human blood platelets. Pretreatment of platelets with stobadine for 30 s inhibited stimulated platelet aggregation in a dose-dependent way. Depending on...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00460-9
更新日期：1999-01-01 00:00:00
abstract：:The regions of muscarinic receptors that specify G-protein-coupling and ligand-binding have been defined in several recent studies. Overall, these studies have shown that amino acids within the third cytoplasmic loop of the receptors define their selectivity for different G-proteins, and that multiple, discontinuous e...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(93)90295-e
更新日期：1993-01-01 00:00:00
abstract：:Nowadays, endocrine disrupting chemical pollution has become one of the major concerns due to the potential role of these chemicals in provoking endocrine disorders especially type 2 diabetes. As a widespread endocrine disrupting chemical, Bisphenol A, with modest estrogenic activity can exert its detrimental effects ...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2018.10.044
更新日期：2018-12-01 00:00:00
abstract：:The discovery of CB1 and CB2 receptors and of endogenous agonists for these receptors has sparked renewed interest in the therapeutic potential of cannabinoids. This has led to a need for strategies that will provide a better separation of wanted from unwanted effects, particularly for CB1 receptor agonists. Possible ...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(99)00282-9
更新日期：1999-01-01 00:00:00
abstract：:A mechanism of antidepressant activity of beta-amyrin palmitate was studied using the forced swimming method in mice. Beta-amyrin palmitate (10 mg/kg) reduced the increase in the duration of immobility induced by tetrabenazine (100 and 200 mg/kg), but showed no effect on that in mice treated with alpha-methyl-para-tyr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90220-w
更新日期：1993-01-01 00:00:00
abstract：:Herba Epimedii is an important medicinal plant which has been used in various traditional Chinese formulations for thousands of years as well as in modern proprietary traditional Chinese medicine products. It has extensive clinical indications, especially for the treatment of sexual dysfunction and osteoporosis. There...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2015.01.006
更新日期：2015-04-01 00:00:00
abstract：:The prokaryotic CRISPR-Cas systems could be applied as revolutionized genome editing tool in live cells of various species to modify, visualize and identify definite sequences of DNA and RNA. CRISPR-Cas could edit the genome by homology-directed repair and non-homologous end joining mechanisms. Furthermore, DNA-target...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118969
更新日期：2021-02-15 00:00:00
abstract：AIM:The present study aimed to understand the relationship between pharmacological activation of mGlu4 receptors and regulation of its gene in the hippocampus. MAIN METHODS:The expression level of the GRM4 gene, encoding mGluR4 receptors, was studied in the hippocampus and frontal cortex of rats after pharmacological ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.03.033
更新日期：2016-05-15 00:00:00
abstract：:Cancer cachexia characterized by a chronic wasting syndrome, involves skeletal muscle loss and adipose tissue loss and resistance to conventional nutritional support. Cachexia is responsible for the reduction in quality and length of life of cancer patients. It also decreases the muscle strength of the patients. The p...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2016.11.033
更新日期：2017-02-01 00:00:00
abstract：:Flavonoids from the leaves of Diospyros kaki L (FLDK-P70) are main therapeutic components of NaoXingQing, which is a novel and patented Traditional Chinese Medicine drug used for the treatment of syndrome of apoplexy for years in China. The present study investigated the effects of FLDK-P70 on hydrogen peroxide (H2O2)...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.09.031
更新日期：2005-03-11 00:00:00
abstract：:The acute dose-dependent effects of epinephrine and cocaine on heart rate and coronary flow rate (CFR) were examined in isolated, perfused (Langendorff) rat hearts from animals: i) pretreated with daily cocaine injections (20 mg/kg/day) for 8 weeks; ii) after 2-day withdrawal from 8-week cocaine pretreatment; iii) veh...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90390-d
更新日期：1990-01-01 00:00:00
abstract：:The colchicine-induced accumulation of vasopressin (AVP) and oxytocin (OXT) has recently been applied to estimate the synthesis and turnover rates for these neuropeptides in whole rat hypothalamus. In the present studies, this pharmacologic procedure has been examined as a potential method for estimating hypothalamic ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90441-d
更新日期：1991-01-01 00:00:00
abstract：AIMS:To investigate the roles of neutrophil alkaline phosphatase (NAP) in the migration, reactive oxygen species (ROS) generation and apoptosis of neutrophils. MAIN METHODS:NAP was overexpressed in neutrophil-like differentiated HL-60 cells via transfecting coding sequence of NAP by lentivirus. NAP overexpression in H...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.06.005
更新日期：2016-07-15 00:00:00
abstract：:Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00389-6
更新日期：1999-01-01 00:00:00
