CRISPR-cas9 genome editing delivery systems for targeted cancer therapy.


:The prokaryotic CRISPR-Cas systems could be applied as revolutionized genome editing tool in live cells of various species to modify, visualize and identify definite sequences of DNA and RNA. CRISPR-Cas could edit the genome by homology-directed repair and non-homologous end joining mechanisms. Furthermore, DNA-targeting modification by CRISPR-Cas methodology provides opportunity for diagnosis, therapy and the genetic disorders investigation. Here, we summarized delivery systems employed for CRISPR-Cas9 for genome editing. Then preclinical studies of the CRISPR-Cas9-based therapeutics will be discussed considering the associated challenges and developments in its translation to clinic for cancer therapy.


Life Sci


Life sciences


Ghaemi A,Bagheri E,Abnous K,Taghdisi SM,Ramezani M,Alibolandi M




Has Abstract


2021-02-15 00:00:00












  • Purification and characterization of enkephalinase, angiotensin converting enzyme, and a third peptidyldipeptidase from rat brain.

    abstract::Three distinct peptidyldipeptidases (exopeptidases releasing carboxyl terminal dipeptide residues) can be solubilized from nerve terminal membrane fractions from whole rat brain or striatum, and separated by ion exchange chromatography. Brain angiotensin-converting enzyme (PDP-1) cleaves Hip-His-Leu, but not 80 nM [3H...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Cushman DW,Gordon EM,Wang FL,Cheung HS,Tung R,Delaney NG

    更新日期:1983-01-01 00:00:00

  • Alpha2-adrenergic agonist modulation of [35S]GTPgammaS binding to guanine-nucleotide-binding-proteins in human platelet membranes.

    abstract::Alpha2-adrenoceptor (alpha2-AR)-regulated binding of the labelled GTP analog, guanosine 5'-O-(3-[35S]thiotriphosphate) ([35S]GTPgammaS), to guanine-nucleotide-binding proteins (G proteins) was studied in human platelet membranes. Under optimal conditions, the potent alpha2-AR agonist, 5-bromo-6-(2-imidazolin-2-ylamino...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Gessi S,Campi F,Varani K,Borea PA

    更新日期:1999-01-01 00:00:00

  • The level of effort, rather than muscle exercise intensity determines strength gain following a six-week training.

    abstract:AIM:This study investigated the effect of voluntary motor effort during a low-intensity (30% maximal voluntary contraction [MVC]) muscle exercise training program on increasing muscle strength. MATERIALS AND METHODS:Eighteen young and healthy individuals were randomly assigned to one of three groups: high mental effor...

    journal_title:Life sciences

    pub_type: 杂志文章,随机对照试验


    authors: Jiang CH,Ranganathan VK,Siemionow V,Yue GH

    更新日期:2017-06-01 00:00:00

  • The effect of diabetes mellitus on aortic prostanoid synthesis and serum cholesterol levels in the rat fed a high cholesterol diet.

    abstract::The effect of diabetes mellitus on serum cholesterol and aortic microsomal prostanoid synthesis was studied in cholesterol fed male Lewis rats. Normal, diabetic and diabetic rats treated with pancreatic islets were divided into three diet subgroups, control diet, control +2% cholesterol for 8 weeks and control +2% cho...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Myers SI,Bartula L,Linzel M,Phan T,Jung PJ,Merrell RC

    更新日期:1990-01-01 00:00:00

  • Effect of lithium on norepinephrine metabolic pathways.

    abstract::We investigated lithium-induced changes in norepinephrine (NE) catabolism. NE and its major metabolites 3-methoxy-4-hydroxyphenylglycol (MHPG) and 3,4-dihydroxyphenyl glycol (DHPG), ions such as lithium (Li(+)), magnesium (Mg(2+)), and potassium (K(+)) were measured in rat plasma and cerebral cortex using an HPLC meth...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sastre E,Nicolay A,Bruguerolle B,Portugal H

    更新日期:2005-07-01 00:00:00

  • Molecular pharmacology of muscarinic receptor heterogeneity.

    abstract::Muscarinic receptors can be pharmacologically classified into 3 types at the present time, however, five genes for the receptor have been identified. The muscarinic receptor types have unique antagonist selectivity, distribution and are linked to specific second messenger systems. The interaction between the muscarini...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Mei L,Roeske WR,Yamamura HI

    更新日期:1989-01-01 00:00:00

  • Vasopressin neuropeptides and acquisition of heroin and cocaine self-administration in rats.

    abstract::The effect of the vasopressin neuropeptide des-glycinamide (Arg8)-vasopressin (DGAVP) on reducing the acquisition of intravenous heroin self-administration in rats was analyzed. When rats reduced in body weight were allowed to self-administer heroin for 1 h per day in the presence of a fixed time, non contingent food ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: van Ree JM,Burbach-Bloemarts EM,Wallace M

    更新日期:1988-01-01 00:00:00

  • Dual endothelin receptor antagonism with bosentan reverses established vascular remodeling and dysfunctional angiogenesis in diabetic rats: relevance to glycemic control.

    abstract:AIMS:We have shown that diabetes causes cerebrovascular remodeling in part by the activation of the endothelin (ET-1) system in a glucose-dependent manner. We also reported increased yet dysfunctional cerebral angiogenesis in diabetes. Here, we tested the hypothesis that dual ET-1 receptor antagonism or glycemic contro...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Abdelsaid M,Kaczmarek J,Coucha M,Ergul A

    更新日期:2014-11-24 00:00:00

  • Two daily melatonin injections differentially induce nonshivering thermogenesis and gonadal regression in the mouse (Peromyscus leucopus).

    abstract::Female white-footed mice (Peromyscus leucopus) were injected twice daily with 5, 10, 50, 100 micrograms melatonin (MEL) or saline. Injections were given for 7 weeks at 2 and 12 hours after lights-on under a long day (LD 16:8) photoperiod. Afternoon administration of MEL induced gonadal regression, although a dose of 5...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Hall ES,Lynch GR

    更新日期:1985-08-26 00:00:00

  • Effects of cocaine hydrochloride on the male reproductive system.

    abstract::The reproductive system effects of cocaine were studied in male rats. The analysis included measurements of circulating levels of luteinizing hormone (LH) and testosterone (T) by radioimmunoassay (RIA). The weights of the testes and sex accessory organs were also assessed and compared with control animals. Dosage leve...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Berul CI,Harclerode JE

    更新日期:1989-01-01 00:00:00

  • Reactive oxygen and nitrogen species modulate the ex-vivo effects of LPS on platelet adhesion to fibrinogen.

    abstract:AIMS:Excessive production of nitric oxide (NO) and reactive oxygen species (ROS) in sepsis modulates different cell functions. Since the sepsis severity is associated with the degree of platelet activation, we decided to investigate the role of systemic generation of NO and ROS in modulating the platelet adhesion of li...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Casarin AL,Lopes-Pires ME,Morganti RP,Antunes E,Marcondes S

    更新日期:2011-11-21 00:00:00

  • Enhanced function of inhibitory presynaptic cannabinoid CB1 receptors on sympathetic nerves of DOCA-salt hypertensive rats.

    abstract:AIMS:This study was performed to examine whether hypertension affects the sympathetic transmission to resistance vessels of pithed rats via inhibitory presynaptic cannabinoid CB1 receptors and whether endocannabinoids are involved in this response. MATERIALS AND METHODS:We compared uninephrectomised rats rendered hype...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Toczek M,Schlicker E,Grzęda E,Malinowska B

    更新日期:2015-10-01 00:00:00

  • Crystal structure of an extended-conformation leucine-enkephalin dimer monohydrate.

    abstract::The structure of a new crystal form of leucine-enkephalin has been determined by X-ray diffraction. There are two independent molecules in the asymmetric unit and both have extended peptide backbone conformations with side-chains arranged alternately above and below the backbone planes. The two pentapeptides are hydro...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mastropaolo D,Camerman A,Ma LY,Camerman N

    更新日期:1987-05-18 00:00:00

  • Thyroid hormone stimulates phosphoglycerate mutase activity and isozyme transition in rat muscle tissues.

    abstract::Triiodothyronine (T3) increases phosphoglycerate mutase (PGAM) specific activity in rat skeletal and cardiac muscles. This increase is concomitant with an increase in the proportion of phosphoglycerate mutase isozymes which contain type-M subunit. Propylthiouracil (PTU), an anti-hormone, not only decreases phosphoglyc...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Esteller M,Ureña J,Carreras J,Martelly I,Climent F

    更新日期:1994-01-01 00:00:00

  • Inhibition of mTORC1/P70S6K pathway by Metformin synergistically sensitizes Acute Myeloid Leukemia to Ara-C.

    abstract:AIMS:Chemo-resistance still was the main obstacle for AML patients, more effective and less toxic forms of therapies were desperately needed. Metformin, a classic hypoglycemic drug for diabetes recently delivered us a new identity that it exerted anti-tumor activity through suppressing mTOR in various tumors. But the a...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yuan F,Cheng C,Xiao F,Liu H,Cao S,Zhou G

    更新日期:2020-02-15 00:00:00

  • Diurnal variations of S-adenosyl-L-methionine and adenosine content in the rat pineal gland.

    abstract::S-adenosylmethionine and adenosine levels in the rat pineal gland were determined by high-performance liquid chromatography after fractionation of the pineal extracts. The concentration of S-adenosylmethionine follows a circadian rhythm and is about three times higher during the day (2.5 nmol/gland) than the night (1....

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chabannes B,Sarda N,Cronenberger L,Pacheco H

    更新日期:1984-08-06 00:00:00

  • Impairment in protein expression profile of testicular steroid receptor coregulators in male rat offspring perinatally exposed to Bisphenol A.

    abstract:AIMS:Steroid hormones and steroid receptors (SRs) play a crucial role in spermatogenesis. Steroid receptor coregulators are the major determinants of SR functioning, and any alteration in their expression is known to be associated with impaired spermatogenesis. Since Bisphenol A (BPA) exposure leads to an impairment of...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Salian S,Doshi T,Vanage G

    更新日期:2009-07-03 00:00:00

  • Effect of fluphenazine on the stimulation of calcium-sensitive phospholipid-dependent protein kinase by 12-O-tetradecanoyl phorbol-13-acetate.

    abstract::The addition of the tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate resulted in activation of calcium-sensitive phospholipid-dependent protein kinase which was dependent on the presence of phospholipid but was essentially independent of calcium. Fluphenazine, which is an effective inhibitor of the ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Donnelly TE Jr,Jensen R

    更新日期:1983-11-28 00:00:00

  • Gene expression of somatostatin receptor subtypes, SSTR1 and SSTR2, in human lung cancer cell lines.

    abstract::Somatostatin (SS) acts as a universal endocrine off-swich, and also inhibits the growth of neuroendocrine tumors through its specific receptors. Small cell lung cancer (SCLC) demonstrates some neuroendocrine characteristics and has been proposed as a candidate for treatment with SS and its analogues. In the present st...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Fujita T,Yamaji Y,Sato M,Murao K,Takahara J

    更新日期:1994-01-01 00:00:00

  • Fenofibrate reduces cardiac remodeling and improves cardiac function in a rat model of severe left ventricle volume overload.

    abstract:AIMS:Fenofibrate is a peroxisome proliferator-associated receptor alpha agonist (PPARα) used clinically for the management of dyslipidemia and is a myocardial fatty acid oxidation stimulator. It has also been shown to have cardiac anti-hypertrophic properties but the effects of fenofibrate on the development of eccentr...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Dhahri W,Couet J,Roussel É,Drolet MC,Arsenault M

    更新日期:2013-01-17 00:00:00

  • Cholinergic drugs ameliorate endothelial dysfunction by decreasing O-GlcNAcylation via M3 AChR-AMPK-ER stress signaling.

    abstract:AIMS:Obesity is associated with increased cardiovascular morbidity and mortality. It is accompanied by augmented O-linked β-N-acetylglucosamine (O-GlcNAc) modification of proteins via increasing hexosamine biosynthetic pathway (HBP) flux. However, the changes and regulation of the O-GlcNAc levels induced by obesity are...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Cui YL,Xue RQ,Xi He,Ming Zhao,Yu XJ,Liu LZ,Wu Q,Si Yang,Li DL,Zang WJ

    更新日期:2019-04-01 00:00:00

  • Effects of bilateral striatal 6-OHDA lesions on circadian rhythms in the rat: a radiotelemetric study.

    abstract::The present work aims to document, in a previously described animal model of Parkinson (double bilateral striatal injection of 6-OHDA), the possible modifications of circadian markers rhythms i.e. temperature (T), heart rate (H) and locomotor activity (A) registered continuously by telemetry, in order to evaluate a po...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Ben V,Bruguerolle B

    更新日期:2000-08-18 00:00:00

  • Effects of smoking marijuana, tobacco or cocaine alone or in combination on DNA damage in human alveolar macrophages.

    abstract::This study examined the role of marijuana smoking in the pathogenesis of human lung cancer by measuring DNA damage in alveolar macrophages (AM). The alkaline unwinding method was used to determine DNA single-strand breaks in AM lavaged from non-smokers [NS] and smokers of marijuana [MS], tobacco [TS] or cocaine [CS], ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sherman MP,Aeberhard EE,Wong VZ,Simmons MS,Roth MD,Tashkin DP

    更新日期:1995-01-01 00:00:00

  • Defining the molecular signatures of human right heart failure.

    abstract:AIMS:Right ventricular failure (RVF) varies significantly from the more common left ventricular failure (LVF). This study was undertaken to determine potential molecular pathways that are important in human right ventricular (RV) function and may mediate RVF. MATERIALS AND METHODS:We analyzed mRNA of human non-failing...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Williams JL,Cavus O,Loccoh EC,Adelman S,Daugherty JC,Smith SA,Canan B,Janssen PML,Koenig S,Kline CF,Mohler PJ,Bradley EA

    更新日期:2018-03-01 00:00:00

  • Contractile responses of the human umbilical artery to KCl and serotonin in Ca-free medium and the effects of levcromakalim.

    abstract::It is known that K(ATP) channel openers inhibit the release and refilling of Ca(2+) from intracellular stores. The present study was designed to test the effects of levcromakalim in human umbilical artery (HUA) rings stimulated by serotonin (5-HT) and KCl in Ca-free medium. Umbilical cords were obtained at vaginal or ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Tufan H,Ayan-Polat B,Tecder-Unal M,Polat G,Kayhan Z,Oğüş E

    更新日期:2003-02-07 00:00:00

  • Nitric oxide production by endothelial cells: comparison of three methods of quantification.

    abstract::Vascular endothelial cells have been found to produce a relaxant mediator, identified as nitric oxide (NO) and implicated in numerous physiological functions. Subsequently, there has been an intensive search for accurate and specific detection methods to measure biological NO production. In the present study, we compa...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Privat C,Lantoine F,Bedioui F,Millanvoye van Brussel E,Devynck J,Devynck MA

    更新日期:1997-01-01 00:00:00

  • Synthetic human pancreatic growth hormone releasing factor (GRF) stimulates growth hormone secretion in the domestic fowl (Gallus domesticus).

    abstract::Synthetic human pancreatic Growth Hormone-Releasing Factor (hpGRF) elevated the plasma concentration of growth hormone (GH) in young and adult domestic fowl. This in vivo effect of hpGRF appeared to be largely similar for both the 32 amino-acid (hpGRF 1-32) or 40 amino-acid (hpGRF 1-40) polypeptide, although the effec...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Scanes CG,Carsia RV,Lauterio TJ,Huybrechts L,Rivier J,Vale W

    更新日期:1984-03-19 00:00:00

  • Dexamethasone and Tofacitinib suppress NADPH oxidase expression and alleviate very-early-onset ileocolitis in mice deficient in GSH peroxidase 1 and 2.

    abstract::C57BL6/J (B6) mice lacking Se-dependent GSH peroxidase 1 and 2 (GPx1/2-DKO) develop mild to moderate ileocolitis around weaning. These DKO mice have a disease resembling human very-early-onset inflammatory bowel disease (VEOIBD), which is associated with mutations in NADPH oxidase genes. Drugs including dexamethasone ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chu FF,Esworthy RS,Shen B,Gao Q,Doroshow JH

    更新日期:2019-12-15 00:00:00

  • Dynamic of plasma IL-22 level is an indicator of thymic output after allogeneic hematopoietic cell transplantation.

    abstract:AIMS:Interleukin-22 (IL-22) promotes thymus recovery and improves T-cell recovery in preclinical allogeneic hematopoietic cell transplant models. However, the correlation between IL-22 and thymus recovery is unknown in human transplant. MATERIALS AND METHODS:In this study, plasma IL-22 levels of transplanted humans we...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Shang L,Duah M,Xu Y,Liang Y,Wang D,Xia F,Li L,Sun Z,Yan Z,Xu K,Pan B

    更新日期:2021-01-15 00:00:00

  • Respiratory responses to opiates applied to the medullary ventral surface.

    abstract::The participation of the medullary structures in the total respiratory effect of opiates was studied by restricting the access of the delta-agonist D-ala2-D-leu5-enkephalin and the mu-agonist D-ala2-Me-Phe4-Met (O) ol5-enkephalin, to the ventral medullary surface, and by comparing their responses with those induced af...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Flórez J,Hurlé MA,Mediavilla A

    更新日期:1982-11-15 00:00:00