CRISPR-cas9 genome editing delivery systems for targeted cancer therapy.

Abstract:

:The prokaryotic CRISPR-Cas systems could be applied as revolutionized genome editing tool in live cells of various species to modify, visualize and identify definite sequences of DNA and RNA. CRISPR-Cas could edit the genome by homology-directed repair and non-homologous end joining mechanisms. Furthermore, DNA-targeting modification by CRISPR-Cas methodology provides opportunity for diagnosis, therapy and the genetic disorders investigation. Here, we summarized delivery systems employed for CRISPR-Cas9 for genome editing. Then preclinical studies of the CRISPR-Cas9-based therapeutics will be discussed considering the associated challenges and developments in its translation to clinic for cancer therapy.

journal_name

Life Sci

journal_title

Life sciences

authors

Ghaemi A,Bagheri E,Abnous K,Taghdisi SM,Ramezani M,Alibolandi M

doi

10.1016/j.lfs.2020.118969

subject

Has Abstract

pub_date

2021-02-15 00:00:00

pages

118969

eissn

0024-3205

issn

1879-0631

pii

S0024-3205(20)31729-X

journal_volume

267

pub_type

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