Abstract：
:Genetically encoded biosensors are useful tools for detecting the presence and levels of diverse biomolecules in living cells. However, low-abundance targets are difficult to detect because they are often unable to bind and activate enough biosensors to detect using standard microscopic imaging approaches. Here we describe a type of RNA-based biosensor, an RNA integrator, which enables detection of low-abundance targets in vitro and in living cells. The RNA integrator is an RNA sequence comprising a ribozyme and an unfolded form of the fluorogenic aptamer Broccoli. Upon binding its target, the ribozyme undergoes cleavage and releases Broccoli, which subsequently folds and becomes fluorescent. Importantly, each target molecule can bind and induce cleavage of multiple copies of the integrator sensor, resulting in an amplified signal. We show that this approach can be generalized to numerous different ribozyme types for the detection of various small molecules.
journal_name
Cell Chem Bioljournal_title
Cell chemical biologyauthors
You M,Litke JL,Wu R,Jaffrey SRdoi
10.1016/j.chembiol.2019.01.005subject
Has Abstractpub_date
2019-04-18 00:00:00pages
471-481.e3issue
4eissn
2451-9456issn
2451-9448pii
S2451-9456(19)30027-3journal_volume
26pub_type
杂志文章abstract：:Alternative polyadenylation (APA) plays a critical role in regulating gene expression. However, the balance between genome-encoded APA processing and autoregulation by APA modulating RNA binding protein (RBP) factors is not well understood. We discovered two potent small-molecule modulators of APA (T4 and T5) that pro...
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pub_type： 杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章,评审
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journal_title：Cell chemical biology
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pub_type： 杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Cell chemical biology
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pub_type： 评论,杂志文章
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
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更新日期：2017-06-22 00:00:00
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journal_title：Cell chemical biology
pub_type： 杂志文章
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abstract：:Epidermal growth factor receptor (EGFR) is a target of signal-derived H2O2, and oxidation of active-site cysteine 797 to sulfenic acid enhances kinase activity. Although a major class of covalent drugs targets C797, nothing is known about its catalytic importance or how S-sulfenylation leads to activation. Here, we re...
journal_title：Cell chemical biology
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