Abstract:
:Ideally, metabolomics should deal with all the metabolites that are found within cells and biological systems. The most common technologies for metabolomics include mass spectrometry, and in most cases, hyphenated to chromatographic separations (liquid chromatography- or gas chromatography-mass spectrometry) and nuclear magnetic resonance spectroscopy. However, limitations such as low sensitivity and highly congested spectra in nuclear magnetic resonance spectroscopy and relatively low signal reproducibility in mass spectrometry impede the progression of these techniques from being universal metabolomics tools. These disadvantages are more notorious in studies of certain plant secondary metabolites, such as saponins, which are difficult to analyse, but have a great biological importance in organisms. In this study, high-performance thin-layer chromatography was used as a supplementary tool for metabolomics. A method consisting of coupling 1H nuclear magnetic resonance spectroscopy and high-performance thin-layer chromatography was applied to distinguish between Ophiopogon japonicus roots that were collected from two growth locations and were of different ages. The results allowed the root samples from the two growth locations to be clearly distinguished. The difficulties encountered in the identification of the marker compounds by 1H nuclear magnetic resonance spectroscopy was overcome using high-performance thin-layer chromatography to separate and isolate the compounds. The saponins, ophiojaponin C or ophiopogonin D, were found to be marker metabolites in the root samples and proved to be greatly influenced by plant growth location, but barely by age variation. The procedure used in this study is fully described with the purpose of making a valuable contribution to the quality control of saponin-rich herbal drugs using high-performance thin-layer chromatography as a supplementary analytical tool for metabolomics research.
journal_name
Planta Medjournal_title
Planta medicaauthors
Ge Y,Chen X,Gođevac D,Bueno PCP,Salomé Abarca LF,Jang YP,Wang M,Choi YHdoi
10.1055/a-0947-5797subject
Has Abstractpub_date
2019-08-01 00:00:00pages
917-924issue
11-12eissn
0032-0943issn
1439-0221journal_volume
85pub_type
杂志文章相关文献
PLANTA MEDICA文献大全abstract::Epilobium sp. are commonly used in traditional medicine in the treatment of early stages of benign prostatic hyperplasia and inflammation. It is suggested that a dominating constituent, oenothein B, is responsible for the extracts therapeutic effects. Several bioactivities were established for extracts and oenothein B...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0043-109372
更新日期:2017-10-01 00:00:00
abstract::Aerial parts of Pterocaulon polystachyum from Chaco province, Argentina, afforded the known coumarins ayapin, isoscopoletin, prenyletin, prenyletin methyl ether, virgatenol, obtusinin, 5-methoxy-6,7-methylenedioxycoumarin, 5-(3,3-dimethylallyloxy)-6,7-methylenedioxycoumarin, 5-(2',3'-dihydroxy-3-methylbutanoxy)-6,7-me...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-2001-17365
更新日期:2001-10-01 00:00:00
abstract::The antiviral activities of two saponins, soyasaponin I and II, isolated from soybean (Glycine max Merrill) were studied in vitro against herpes simplex virus type 1 (HSV-1). Soyasaponin II was more potent than soyasaponin I as shown by reduction of HSV-1 production. Soyasaponin II was also found to inhibit the replic...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957622
更新日期:1997-04-01 00:00:00
abstract::The essential oils of Salvia ringens (samples A and B), were analyzed by means of GC and GC/MS. From the seventy-five identified constituents representing 99.82 and 99.86% of the oils, 1,8-cineole and alpha-pinene were the major components. Furthermore, sample B exhibited a very interesting antimicrobial profile after...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-2001-10627
更新日期:2001-02-01 00:00:00
abstract::Many herbs, and recently their biomass from in vitro cultures, are essential for the treatment of diseases. The aim of this study was to determine the optimal growth of Bacopa monnieri (water hyssop) in an in vitro culture and to examine if extracts of the B. monnieri biomass from the in vitro culture would affect hyd...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0035-1558166
更新日期:2016-02-01 00:00:00
abstract::In Brazil, Plectranthus species are known as "boldo" and have been used in popular medicine for analgesic and dyspeptic purposes. Plectranthus need to be well identified in order to be used as commercially genuine medicinal plants. Here we describe AFLP DNA patterns able to distinguish among different Pectranthus spec...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-946673
更新日期:2006-08-01 00:00:00
abstract::Two new lactones, 1 and 2, together with five known compounds, 3-7, were isolated from the marine mangrove fungus Xylaria sp. BL321. Their structures were determined by comprehensive analysis of their MS and NMR spectroscopic data. The absolute configurations of 1 and 2 were established on the basis of electronic circ...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-0031-1280347
更新日期:2012-01-01 00:00:00
abstract::In natural product chemistry, it is often crucial to determine sugar composition as well as the absolute configuration of each monosaccharide in glycosides. An ultra-performance liquid chromatography method using both photodiode array (PDA) and mass spectrometry detectors (UPLC-UV/MS) was developed for qualitative ana...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0031-1298432
更新日期:2012-05-01 00:00:00
abstract::This perspective describes some commonly used animal models for the evaluation of potential chemopreventive agents in colon carcinogenesis. Special emphasis is given to the azoxymethane (AOM)/1,2-dimethylhydrazine (DMH) rat model and APCMIN mice, carrying a mutation in APC, a key gene in human carcinogenesis. In the A...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2008-1074577
更新日期:2008-10-01 00:00:00
abstract::The volatile constituents of ZIZIPHORA TAURICA subsp. CLEONIOIDES (Boiss.) P. H. Davis (Lamiaceae) were studied for the first time by analysis of the essential oil. 33 compounds, from the 44 consisting the total of the fraction, are identified by means of capillary GC and GC-MS. The identified components represent abo...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-962379
更新日期:1988-04-01 00:00:00
abstract::We describe in this paper that the alkaloid 4-methylaaptamine, isolated from the marine sponge Aaptos aaptos, inhibited HSV-1 infection. We initially observed that 4-methylaaptamine inhibited HSV-1 replication in Vero cells in a dose-dependent manner with an EC50 value of 2.4 microM. Moreover, the concentration requir...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-967109
更新日期:2007-03-01 00:00:00
abstract::Esters of 13-hydroxylupanine and 4-hydroxylupanine with acetic, propionic, butyric, isobutyric, valeric, isovaleric, tiglic, benzoic, and TRANS-cinnamic acid have been synthesized and characterized by capillary gas-liquid chromatography and mass spectrometry (EI-MS, CI-MS). In LUPINUS POLYPHYLLUS, L. ALBUS, L. ANGUSTI...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-962415
更新日期:1988-06-01 00:00:00
abstract::Three new secoiridoid glucosides, jasamplexosides A, B and C, were isolated from the crude drug "Niu du teng", the leaves and stems of JASMINUM AMPLEXICAULE, together with the known secoiridoid glucosides, 10-hydroxyligstroside and jasminoside. Their structural elucidation by spectroscopic studies is described. ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-961548
更新日期:1992-12-01 00:00:00
abstract::Galphimine-B (G-B) is a bioactive compound isolated from the plant Galphimia glauca Cav. (Malpighiaceae) with central nervous system depressant properties previously described. In the present study, extracellular spiking activity records in either somatosensorial cortex or ventral tegmental area (VTA) neurons, were pe...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957440
更新日期:1998-05-01 00:00:00
abstract::Volatile oils of the leaves, fruits and stem-bark of CLEISTOPHOLIS PATENTS Engl. et Diels. (Annonacae) were analysed by means of gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of sixty-one components were identified; qualitative and quantitative differences in the oil composition wer...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-962450
更新日期:1988-08-01 00:00:00
abstract::Casearin X (CAS X) is the major clerodane diterpene isolated from the leaves of Casearia sylvestris and has been extensively studied due to its powerful cytotoxic activity at low concentrations. Promising results for in vivo antitumor action have also been described when CAS X was administered intraperitoneally in mic...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/a-0765-9523
更新日期:2019-03-01 00:00:00
abstract::The chloroform extract of Valeriana officinalis led to the isolation of clionasterol-3-O-beta-D-glucopyranoside and a mixture of 6'-O-acyl-beta-D-glucosyl-clionasterols. The acyl moieties were identified as hexadecanoyl, 8 E,11 E-octadecadienoyl and 14-methylpentadecanoyl by alkaline hydrolysis followed by GC-MS analy...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2005-864179
更新日期:2005-10-01 00:00:00
abstract::The phenylpropanoid dibenzylbutyrolactone lignan arctigenin, a key component found in Arctium lappa, or burdock, has been reported with a variety of therapeutic effects including anticancer, anti-inflammation, and antivirus effects. Using LC/MS/MS, three novel metabolites of arctigenin, namely, arctigenic acid, arctig...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0032-1328332
更新日期:2013-04-01 00:00:00
abstract::The cytotoxicity of goniothalamin was found to be strong towards both cancerous (HGC-27, MCF-7, PANC-1, HeLa), and non-cancerous (3T3) cell lines, especially in cases of dividing cells. Drug exposure studies indicated that the cytotoxic action of goniothalamin was time- and dose-dependent. At the ultrastructural level...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957611
更新日期:1997-02-01 00:00:00
abstract::Callus induction starting from the rhizome tissue of plants of three CURCUMA species: CURCUMA ZEDOARIA Roscoe, C. DOMESTICA Valeton, and C. AROMATICA Salisbury (Zingiberaceae) was performed. Successful callus formation was obtained in all three species using Murashige-Skoog's (MS) medium supplemented with 1-naphthalen...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-962344
更新日期:1988-02-01 00:00:00
abstract::Cell Suspension cultures of THAMNOSMA MONTANA were grown in Gamborg B5 medium which was best suited for acridone alkaloid formation. From the lyophilized cell material nine acridones and three acridone-monoglucosides have been isolated which were found for the first time in the genus THAMNOSMA. Moreover, N-methylacrid...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-959437
更新日期:1994-04-01 00:00:00
abstract::The distribution and level of yew constituents vary with species and tissues. In this study, a rapid and valid method incorporating ultra-fast liquid chromatography (UFLC) with MS and UV detection was developed for simultaneous determination of paclitaxel and its six semisynthesis precursors in needles and hair roots ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0030-1249959
更新日期:2010-10-01 00:00:00
abstract::Comparative study of the seeds of two Cucurbitaceae Momordica charantia L. and Herpetospermum caudigerum Wall. shows and important difference for their metabolism concerning particularly aspartic acid. Momordica can produce vicin, while for Herpetospermum, this amino acid would give asparagin. In the other hand, betwe...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-971211
更新日期:1982-11-01 00:00:00
abstract::The ethanolic extract of Ilex aquifolium L. (Aquifoliaceae) concentration-dependently inhibited leukotriene B4 biosynthesis in isolated bovine PMNL with an IC50 value of about 60 micrograms/ml, whereas the effect on epidermal 12(S)-HETE biosynthesis was much less pronounced. The extract also inhibited the non-enzymati...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957509
更新日期:1998-08-01 00:00:00
abstract::Reinvestigating the constituents of the roots of Vitis thunbergii Sieb. & Zucc. led to the isolation of three new resveratrol derivatives, vitisinols E-G (1-3), together with 14 known compounds. The structures of compounds 1-3 were established by application of spectroscopic analyses (NMR, MS, UV, and IR). ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0029-1185404
更新日期:2009-06-01 00:00:00
abstract::Based on rDNA ITS sequences of D. officinale and the other 37 species of Dendrobium, a pair of allele-specific diagnostic primers, TP-JB01S and TP-JB01X, were designed to authenticate D. officinale from the other species. Before the diagnostic PCR, the primer pair, P1 and P2, for amplifying the whole ITS region was us...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2003-40651
更新日期:2003-06-01 00:00:00
abstract::Twelve phenolic compounds, including three stilbenes, two flavonoids, two coumarins, one neolignan, and four lignans, isolated from Euphorbia and Pycnanthus species or obtained by derivatization, were assayed for their potential antineoplastic efficacy in three human cancer cell lines: gastric (EPG85-257), pancreatic ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0029-1240892
更新日期:2010-07-01 00:00:00
abstract::Two new protopine-type alkaloids, argemexicaine A (1) and argemexicaine B (2), along with thirteen known alkaloids, were isolated from MeOH extracts of Formosan Argemone mexicana L. (Papaveraceae). Physical and spectral analyses, particularly IR and thermo-modulated 1D and 2D NMR, were used to determine the transannul...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2003-37710
更新日期:2003-02-01 00:00:00
abstract::During the screening of antimalarial substances, the 80% EtOH extract from the outer bark of Ochna integerrima Merr. (Ochnaceae) was shown to have a good anti-malarial activity (IC50 value: 6.5 microg/mL) whereas extracts from the inner barks of O.integerrima showed no antimalarial activity. Biflavanone (1), which had...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-931569
更新日期:2006-06-01 00:00:00
abstract::During the seasons 1989-1993, Valeriana officinalis plants were investigated for their contents of essential oil, valerenic acid and derivatives, and valepotriates. Harvesting of the subterranean parts was started in August of the year in which the seeds were sown, and continued until the last week of April of the sub...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957392
更新日期:1998-03-01 00:00:00