Abstract:
:Currently it is not feasible to isolate sufficient numbers of islets from a single pancreas for clinical transplantation. We examined whether small numbers of islets obtained from multiple donors could be used for transplantation. Islets were isolated from four inbred strains of mice (DBA/2, DBA/1, C3H, and A.SW) by a stationary collagenase digestion and Ficoll separation and transplanted into the renal subcapsular space of streptozotocin-induced diabetic B6AF1 mice. At least 200 handpicked islets were required to produce normoglycemia in syngeneic and allogeneic diabetic recipient mice. None of the mice given 50 islets became normoglycemic within 2 weeks postgrafting. When various numbers of purified islets from a single donor were transplanted, the survival was significantly better for 200-islet allografts than for 800-islet and 400-islet allografts. When a 200-islet composite graft was prepared from four donors (50 fresh handpicked islets from each donor), the survival of the composite graft as measured by sustained normoglycemia in nonimmunosuppressed recipients was dramatic, with 17 of 18 recipients maintaining normoglycemia indefinitely (greater than 200 days). Similarly, when islets isolated from four donors and cultured for various periods were mixed and transplanted (200 islets/recipient) all recipient mice (n = 8) enjoyed indefinite graft survival. Use of higher numbers of purified islets or crude islets in a composite multiple-donor islet allograft was less effective in achieving indefinite graft survival. Thus, transplantation of a composite graft made up with subtherapeutic numbers of islets from multiple histoincompatible donors to provide adequate therapeutic numbers is a practical solution to the lack of islet availability. In addition, composite islet grafts appear to possess immunological advantages, with significantly prolonged survival over that produced by single-donor islet grafts.
journal_name
Transplantationjournal_title
Transplantationauthors
Gotoh M,Porter J,Kanai T,Monaco AP,Maki Tsubject
Has Abstractpub_date
1988-06-01 00:00:00pages
1008-12issue
6eissn
0041-1337issn
1534-6080journal_volume
45pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Humanized Fc receptor (FcR)-nonbinding antibodies against CD3 are promising immunosuppressive agents that may overcome both the neutralizing response to and the cytokine release syndrome seen with conventional monoclonal antibodies against CD3. In addition, evidence from several murine models suggests that t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200104150-00020
更新日期:2001-04-15 00:00:00
abstract:BACKGROUND:Combined lung and liver transplantation (Lu-LTx) is a therapeutic option for selected patients with coexisting lung and liver disease. For several reasons, Lu-LTx is performed in few centers and information about the technical issues, posttransplant management and long-term outcomes associated with this proc...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181636f3f
更新日期:2008-02-27 00:00:00
abstract::Low-affinity, cold-reactive antibodies easily removed by washing were not detected by the antiglobulin technique but killed T lymphocytes when washing was omitted, incubation was prolonged, and cytotoxic tests were incubated at room temperature or at 4 degrees C. These antibodies were present in approximately 25% of s...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198710000-00014
更新日期:1987-10-01 00:00:00
abstract:BACKGROUND:Connective tissue growth factor (CTGF) is a key mediator of tissue fibrogenesis in kidney disease. Its involvement in renal allograft fibrosis was recently demonstrated in a mouse model. METHODS:We prospectively studied the association between urinary CTGF (CTGFu) levels and renal allograft fibrosis during ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31829b07e1
更新日期:2013-09-15 00:00:00
abstract::In 2007, the islet community was notified that the collagenase product most commonly used for human islet isolations contained bovine neural tissue contaminants. To minimize this potential hazard, we adapted our human islet processing procedure to use a GMP-manufactured, bovine neural tissue-free collagenase blend. He...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0b013e3181b443ae
更新日期:2009-09-27 00:00:00
abstract::In light of the transplant community's efforts to increase living donation, preserving donor autonomy is essential. Understanding how often and the reasons why potential living kidney donors opt out of the donor evaluation process for nonmedical reasons is important for assessing donor voluntariness. The experiences o...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0000000000001408
更新日期:2016-12-01 00:00:00
abstract::We have studied organ allograft survival in rhesus monkeys conditioned with myeloablative total-body irradiation and T cell-depleted autologous bone marrow transplantation then given a heterotopic MHC-mismatched cardiac allograft in the immediate postmyeloablative period. This model has enabled us to investigate the r...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198911000-00009
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND:Hepatic artery thrombosis (HAT) is a significant cause of morbidity after liver transplantation. The aims of this study are to identify and compare risk factors that might contribute to HAT. METHODS:A total of 424 liver transplants performed at the University of Virginia were reviewed. HAT was defined as co...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200103270-00014
更新日期:2001-03-27 00:00:00
abstract::Increased QT dispersion (QTd), predicting patients with risk of malignant arrhythmia, have recently been reported in hemodialysis patients (HDp). In this prospective study, we aimed to investigate changes in QTd and signal averaged-ECG (SAECG) in HDp after transplantation. Twenty-seven HDp (M/F:18/9, mean age 30+/-8 y...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/00007890-200111150-00009
更新日期:2001-11-15 00:00:00
abstract::Although bisphosponates are proposed as first-line treatment for posttransplant bone disease they are not optimal in all situations. A kidney transplant recipient developed hypercalcemia from mobilization of extraskeletal calcium. He had low serum parathyroid hormone and vitamin D; high calcium excretion; and normal c...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200106270-00025
更新日期:2001-06-27 00:00:00
abstract:BACKGROUND:Remote ischemic preconditioning (RIPC) protects against liver ischemia reperfusion (IR) injury. An essential circulating mediator of this protection is nitric oxide (NO) induced by lower limb RIPC. One of the mechanisms through which NO generally acts is the soluble guanylyl cyclase-cyclic GMP (sGC-cGMP) pat...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31824cd59d
更新日期:2012-05-15 00:00:00
abstract:BACKGROUND:The effective use of new steroid-sparing immunosuppressive regimens may lower cumulative glucocorticoid use among renal transplant recipients. However, it is unknown what effect this therapeutic trend has had on bone disease. METHODS:Unselected newly transplanted inpatients (n=45) were identified and compre...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200301150-00009
更新日期:2003-01-15 00:00:00
abstract:BACKGROUND:Posttransplant lymphoproliferative disease (PTLD) is a major complication after bone marrow and solid organ transplantation. The disease encompasses a spectrum of abnormal, Epstein-Barr virus (EBV)-associated B-cell proliferations. We have previously shown that EBV-infected, spontaneous lymphoblastoid cell l...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200208150-00017
更新日期:2002-08-15 00:00:00
abstract:BACKGROUND:The timing of an acute rejection may have a variable impact on renal allograft survival. To determine whether the time of first acute transplant rejection (ATR) is an independent predictor of long-term allograft survival, we studied 31,600 first cadaveric renal transplants that were functional on the first t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199705150-00013
更新日期:1997-05-15 00:00:00
abstract:BACKGROUND:New insights into mechanisms of injury in orthotopic liver transplantation (OLT) often come from experiments in small animal models. Mice are particularly suitable because of the growing availability of gene-altered animals and specific antibodies. A validated model of OLT in mice is not available and, in pa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200211150-00008
更新日期:2002-11-15 00:00:00
abstract:BACKGROUND:It remains unknown whether low-grade hyperparathyroidism persisting beyond the first year postrenal transplantation has any impact on bone density. METHODS:Parathyroid hormone (PTH), glomerular filtration rate (GFR), and bone density (by dual-energy x-ray absorptiometry [DXA]) were monitored in the follow-u...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000230130.50451.78
更新日期:2006-09-27 00:00:00
abstract:BACKGROUND:The long-term outcome of simultaneous kidney pancreas transplant recipients is not well established. METHODS:We retrospectively reviewed all patients who underwent simultaneous kidney-pancreas transplantation with bladder drainage at our center between January 1989 and December 1991. A total of 57 patients ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200002270-00015
更新日期:2000-02-27 00:00:00
abstract::Graft rejection remains an important cause of renal allograft failure, despite improvements in immunosuppression and HLA typing. Although HLA matching is beneficial, ensuring an exact match it is often impractical. Thus, a reliable in vitro method for quantitating and qualitating alloreactivity is an important goal. I...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199510150-00015
更新日期:1995-10-15 00:00:00
abstract:BACKGROUND:There is an increasing appreciation of the deleterious effects of antibody and B cells on acute and chronic transplant outcomes. Many effector functions of antibody are mediated by a family of receptors (FcγRs) that are expressed on most immune cells, including neutrophils, natural killer cells, and B cells....
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000287
更新日期:2014-08-15 00:00:00
abstract::We have previously demonstrated that streptozotocin-induced diabetic rats have decreased guanylate cyclase (EC 4.6.1.2) activity in liver and other tissues which was returned to normal by the administration of exogenous insulin. Since successful pancreatic islet transplants have been shown to lower basal hepatic gluco...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract:BACKGROUND:In nontransplant patients with chronic hepatitis C virus (HCV), HCV genotype has been linked with a differential response to antiviral therapy, risk of steatosis and fibrosis, as well as all-cause mortality, but the role of HCV genotypes in posttransplant disease progression is less clear. METHODS:Using the...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/TP.0000000000000413
更新日期:2015-04-01 00:00:00
abstract::One major limitation in pancreatic islet transplantation is availability of donor tissue. Donor shortage is exacerbated by islet apoptosis from the stresses of islet isolation and transplantation. Furthermore, the side effects of immunosuppressive drugs preclude transplants into patients whose diabetes is controlled b...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000132283.95107.9c
更新日期:2004-05-27 00:00:00
abstract::The class II region of major histocompatibility complex of the rat. Rattus norvegicus (RT1) consists of RT1.B, RT1.D, and RT1.H subregions. The gene order around the H subregion was determined as RT1.A--H beta-H alpha--B by RFLP analysis of naturally occurring intra-RT1 recombinant rats with HLA DP probes. A unique re...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199108000-00035
更新日期:1991-08-01 00:00:00
abstract::The mammalian target of rapamycin inhibitors (mTOR-I), drugs widely used in transplant medicine and oncology, exert their function by inhibiting a serine/threonine kinase with a pivotal role in cellular metabolism and in a wide range of eukaryotic biological/cellular functions and signaling networks. Additionally, as ...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0000000000001806
更新日期:2018-02-01 00:00:00
abstract::It has been postulated that the establishment of acquired, neonatal immunologic tolerance is due to a "deficit" in interleukin 2 (IL-2). To test this hypothesis, chickens were made immunologically tolerant to both major and minor histocompatibility antigens by transplantation of skin grafts onto newly hatched recipien...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198802000-00041
更新日期:1988-02-01 00:00:00
abstract:BACKGROUND:Pneumocystis jirovecii pneumonia (PJP) is an important infection-related complication, whose mode of transmission remains uncertain. METHODS:We investigated a nosocomial cluster of 14 PJP cases (11 confirmed and 3 probable) in kidney transplant recipients using epidemiological and genotyping methods. RESUL...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182384b57
更新日期:2011-12-27 00:00:00
abstract:INTRODUCTION:This is a follow-up of a withdrawal study that we previously performed on 104 liver transplant patients in which immunosuppression was gradually withdrawn over a period of 3 years. Eighty-one patients were not able to be withdrawn (rejectors), and 23 patients were successfully weaned off immunosuppression ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182003db7
更新日期:2010-12-27 00:00:00
abstract:BACKGROUND:Graft-versus-host disease (GVHD) is a major complication after bone marrow transplantation, which may be refractory to immunosuppressive drugs. As preliminary case reports suggested that extracorporeal photochemotherapy (ECP) using a Therakos device might be beneficial, we conducted a pilot study to assess t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199707150-00010
更新日期:1997-07-15 00:00:00
abstract:BACKGROUND:Identification of risk factors for BK virus (BKV) replication may improve transplant outcome. We investigated the impact of immunosuppressive drugs on the prevalence of BKV replication in recipients of human renal allografts. METHODS:One hundred twenty renal allograft recipients were studied prospectively a...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31817c6447
更新日期:2008-08-27 00:00:00
abstract:BACKGROUND:It is unclear to what extent cancer history affects posttransplantation mortality in solid organ transplant recipients. METHODS:We identified a Swedish population-based cohort of solid organ transplant recipients in the National Patient Register 1970 to 2008 and linked it to the Cancer and Cause-of-Death Re...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31829854b7
更新日期:2013-08-15 00:00:00